(1) Background: Few studies to date have assessed the influences induced by climate change on the spatial distribution and population abundance of Aedes albopictus using the latest climate scenarios. In this study, we updated the current distribution of Ae. albopictus mosquitoes and evaluated the changes in their distribution under future climate conditions, as well as the risk of dengue virus emergence in Romania. (2) Methods: Under the two scenarios: High scenario (HS) when no drastic measures to reduce the effects of global warming will be taken, or they are not effective and low scenario (LS) when very stringent greenhouse control measures will be implemented. (3) Results: The results estimate an increase in temperatures in Romania of up to 2.6 °C in HS and up to 0.4 °C in LS, with an increase in the period of virus replication within the vector from June to October in HS and from May to September in LS. Moreover, in 2022, Ae. albopictus was reported in a new county, where it was not identified at the last monitoring in 2020. (4) Conclusions: The rapid spread of this invasive species and the need to implement monitoring and control programs for the Aedes population in Romania are emphasized.

ESKAPE pathogens are considered as global threats to human health. The discovery of new molecules for which these pathogens have not yet developed resistance is a high medical priority. Synthetic flavonoids are good candidates for developing new antimicrobials. Therefore, we report here the potent in vitro antibacterial activity of BrCl-flav, a representative of a new class of synthetic tricyclic flavonoids. Minimum inhibitory/bactericidal concentration, time kill and biofilm formation assays were employed to evaluate the antibacterial potential of BrCl-flav. The mechanism of action was investigated using fluorescence and scanning electron microscopy. A checkerboard assay was used to study the effect of the tested compound in combination with antibiotics. Our results showed that BrCl-flav displayed important inhibitory activity against all tested clinical isolates, with MICs ranging between 0.24 and 125 µg/mL. A total kill effect was recorded after only 1 h of exposing Enterococcus faecium cells to BrCl-flav. Additionally, BrCl-flav displayed important biofilm disruption potential against Acinetobacter baumannii. Those effects were induced by membrane integrity damage. BrCl-flav expressed synergistic activity in combination with penicillin against a MRSA strain. Based on the potent antibacterial activity, low cytotoxicity and pro-inflammatory effect, BrCl-flav has good potential for developing new effective drugs against ESKAPE pathogens.

This study describes granulocytic anaplasmosis in two dogs naturally infected with Anaplasma phagocytophilum. The 3-year-old dogs (male and female) came from the same household and were referred to the Faculty of Veterinary Medicine in Iasi for blood donation. They were subject to standard routine tests: haematology blood test, blood smear, and serological tests (VETSCAN^® FLEX4 and IDEXX SNAP 4Dx Plus). The female dog had no medical problems, while the male dog experienced joint pain. The blood smear was negative for tick-borne pathogens, and the haematology findings indicated thrombocytopenia in both dogs, with the male dog also displaying eosinophilia. The two dogs were mildly positive in the ELISA tests for the detection of Anaplasma spp. antibodies; therefore, the blood samples were tested using the qRT-PCR method for Anaplasma platys and Anaplasma phagocytophilum. The qRT-PCR result was negative for A. platys, but it was positive for A. phagocytophilum. The treatment consisted of the administration of doxycycline for 28 days. In conclusion, the high number of cases with non-specific clinical signs, the different sensitivity and specificity of the immunochromatographic serological tests, as well as the possibility of confusing the morula during the cytological examination, make the molecular test mandatory for precise diagnosis.

West Nile virus (WNV) is a re-emerging zoonotic pathogen that represents a threat to both animal and human health. It is difficult to estimate the impact of WNV in the future, although many of the climatic factors influencing its spread have been identified. In this study, we used bioclimatic indices to estimate those periods that favour the growth of vector mosquito populations and the incubation periods for the virus. To this end, we studied the climatic changes in the Romanian regions where cases of WN infection have been reported. Simulations were carried out for 2100 based on long-term scenarios. Identifying the bioclimatic conditions which can cause WNV outbreaks in Romania is necessary to anticipate and thereby prevent future epidemics. However, no extraordinary weather events were registered in the years with WNV outbreaks which could explain such a high number of cases. Thus, in the High Scenario (which will occur if actions to control (GHG) gas emissions are not taken or implemented effectively), the hatching period is extended until November, with the risk that adult mosquitoes are active throughout the year, ensuring a high survival rate of the virus within mosquitoes. In addition, in the High Scenario, the transmission period of the virus is extended from April to October, which underlines the need to establish monitoring and control programmes for both mosquito populations and the spread of the virus among the animal and human populations.

The results regarding the prevalence of toxoplasmosis in humans in the city of Iasi during one year, show a rate of 6,6% of cases detected with positive IgM, and 32.7% of cases detected with positive IgG, compared to the prevalence of toxoplasmosis in cats which shows a rate of 0.7% of positive cases detected with positive IgM; which denotes that toxoplasmosis is underdiagnosed in veterinary medicine. A very important role is played by the diagnostic method used. The https://www.uaiasi.ro/revmvis/index_htm_files/Article deals with a case study, a 1,8-year-old cat with cerebellar ataxia, dysmetria and hypermetria, with moderate opacification of the entire corneal surface, panuveitis, chorioretinitis and corneal edema. Following the paraclinical investigations, the diagnosis of toxoplasmosis was made, using the Welltest Toxoplasma IgG/IgM immunochromatographic test, confirming the acute phase of the disease with positive IgM and negative IgG. Using the molecular detection techniques through qRT PCR, the result was negative, emphasizing the fact that the protozoan Toxoplasma gondii uses the blood as a way of spreading in the body, the relatively short phase that can induce a negative result, despite the presence of severe symptoms. The conclusions emphasize the importance of using a correct diagnostic method, molecular techniques, despite their high sensitivity, are not always recommended. In toxoplasmosis, the recommended diagnostic method is the serological one to detect IgG/IgM antibodies.