Considering the effects of rapid population growth, urbanisation and climate change in recent years, the protection of freshwater resources, the prevention of water pollution and the proper sharing of freshwater resources among different sectors have become important issues. Water footprint (WF) is a sign of freshwater use and is not only an indicator that can be used in the climate crisis, but also to protect water against nitrate pollution. In this study, the Küçük Menderes Basin was chosen as the study area due to different crop varieties. Agricultural crop patterns were classified using Rapideye and Sentinel-2 satellite images of the study area obtained in 2017. Thus, the cultivated areas were obtained for cotton and maize (grain and silage) in the basin. In particular, agricultural crop patterns were considered in which agricultural production was intensive and blue water was used predominantly. As a result, the first-crop corn production, which has a high blue WF of 3840 m³/ton in the basin, has the highest greywater footprint due to the use of intensive chemical fertilisers. This was followed by cotton with 2331 m³/ton, and the second-crop silage corn production had the lowest greywater footprint. Agriculture’s water footprint assessment provides a solid foundation for planning climate change adaptive crop production, managing nitrate-sensitive areas and anticipating future regional changes.

Edible halophytes are attracting attention due to their potential for agriculture in saline and marginal areas. The salt tolerance mechanism was analyzed in Crithmum maritimum L., based on ionic, osmotic, and redox homeostasis strategies under salt stress. The methodology involved growing C. maritimum seeds in pots under controlled greenhouse conditions and exposing them to different NaCl concentrations (0, 100, 200, and 300 mM) for five months. High salinity levels decreased plant length and biomass, but the shoot-to-root length and biomass ratio increased significantly. Photosynthetic pigments (chlorophyll and carotenoids) were quantified using spectrophotometric analysis, while macro- and micro-nutrient contents were determined via the Kjeldahl method, flame photometry, and atomic absorption spectrophotometry. Osmolyte accumulation, including proline and glycine betaine, was analyzed using specific biochemical assays, and antioxidant enzyme activities (SOD, CAT, and POX) were measured to assess redox homeostasis. Photosynthetic pigments in C. maritimum leaves slightly increased at 100 mM NaCl, but significantly declined at 200 and 300 mM NaCl. A high Na content in the shoots indicated no restriction in mineral uptake in the roots. Nitrogen and phosphorus slightly decreased under high salinity. The cation content in the shoots varied: potassium decreased, while calcium and magnesium increased with salinity, although the Mg⁺²/Na⁺ and K⁺/Na⁺ ratios showed similar declining patterns. The micro-nutrients iron and manganese increased in the shoots, while copper remained unchanged. The content of osmolytes proline and glycine betaine significantly increased under the 200 and 300 mM NaCl treatments. Antioxidant enzyme activities (SOD, CAT, and POX) decreased at 100 and 200 mM NaCl, but were strongly induced at 300 mM NaCl. The total antiradical activity of the leaves increased with higher salinity levels. Our results indicated that the facultative halophyte characteristics of C. maritimum emerged after exposure to 200 mM NaCl. Increased calcium content may be a key factor in salinity tolerance. We concluded that C. maritimum employs strong osmotic adjustment and redox homeostasis mechanisms, making it a promising candidate for cultivation in saline environments.