To explore the feasibility of dietary Chinese herbal residue (CHR) supplementation in swine production with the objective of valorization, we examined the effects of dietary supplementation with CHR or fermented CHR products on the colonic ecosystem (i.e., microbiota composition, luminal bacterial metabolites, and expression of genes related to the intestinal barrier function in weaned piglets). We randomly assigned 120 piglets to one of four dietary treatment groups: a blank control group, CHR group (dose of supplement 4 kg/t), fermented CHR group (dose of supplement 4 kg/t), and a positive control group (supplemented with 0.04 kg/t virginiamycin, 0.2 kg/t colistin, and 3000 mg/kg zinc 0.04 kg/t virginiamycin, 0.2 kg/t colistin, and 3000 mg/kg zinc oxide). Our results indicate that dietary supplementation with CHR increased (P < 0.05) the mRNA level corresponding to E-cadherin compared with that observed in the other three groups, increased (P < 0.05) the mRNA level corresponding to zonula occludens-1, and decreased (P < 0.05) the quantity of Bifidobacterium spp. When compared with the blank control group. Dietary supplementation with fermented CHR decreased (P < 0.05) the concentration of indole when compared to the positive control group; increased (P < 0.05) the concentrations of short-chain fatty acids compared with the values measured in the CHR group, as well as the mRNA levels corresponding to interleukin 1 alpha, interleukin 2, and tumor necrosis factor alpha. However, supplementation with fermented CHR decreased (P < 0.05) interleukin 12 levels when compared with the blank control group. Collectively, these findings suggest that dietary supplementation with CHR or fermented CHR modifies the gut environment of weaned piglets.

This study was conducted to determine the effects of diet supplementation of laying hens with antimicrobial peptides (AMP) on egg production, egg quality and caecal microbiota. A total of 360 Hy‐Line Brown laying hens (72 weeks old) were divided into three groups with four replicates of 30 birds each. The laying hens were fed with the basal diet (Control), the basal diet + 50 mg/kg AMP (group 1) and the basal diet + 100 mg/kg AMP (group 2). The experiment lasted for 45 d. Eggs were collected daily and caecal samples were collected at the end of the experiment. The results showed that AMP supplementation caused a significantly increased laying rate and decreased feed/egg ratio (p ＜ .05). Meanwhile, a distinctive difference in cecal microbiota was observed between AMP and control groups and the average values of microbial diversity and richness were lower in the AMP group than in the control group. At the phylum level, the relative abundance of Verrucomicrobia and Cyanobacteria were lower in the AMP group than in the control group. In conclusion, the results indicated that dietary supplementation with AMP can improve egg production and affect the cecal microbial community membership and structure of hens during late laying period.

The effects of Hermetia illucens larvae meal (HILM) as a feed supplement on production performance and cecal microflora were studied in 900 Hy-line Brown laying hens. Laying hens (60 weeks old) were randomly divided into four groups. Each group had five replicates, and each replicate had 45 hens. The control group was fed with a corn–soybean-based diet, and the experimental groups were fed with 1% HILM, 2% HILM, or 3% HILM. Results were as follows: (1) With the increase in HILM level, the laying rate increased linearly (p ≤ 0.05), and the feed/egg and cracked-egg rate decreased linearly (p ≤ 0.05). (2) Community composition analysis showed that the dominant bacteria in each group were Bacteroidetes and Firmicutes, followed by Actinobacteria and Proteobacteria, which accounted for more than 97% of 16S rRNA gene sequence of the total cecal bacteria. (3) Alpha diversity analysis at the operational taxonomic unit classification level showed that the HILM-addition groups had higher community richness and community diversity than the control group. (4) Principal co-ordinates analysis showed that the cecum samples in each group were significantly separated (p ≤ 0.05). At the phylum level, the relative abundance of Bacteroidetes in the HILM addition groups was significantly lower than that in the control group (p < 0.001), and the relative abundance of Firmicutes in the HILM addition groups was significantly higher than that in the control group (p < 0.001). In conclusion, dietary HILM supplementation had a significant effect on the production performance and cecal microflora of laying hens at the late laying period under the conditions of this experiment but had no adverse effect on the intestinal dominant flora.

This study was conducted to determine the effects of different proportions of Broussonetia papyrifera (BP)-fermented feed in the diet to replace part of soybean meal on the production performance, egg quality and caecal microbiota of laying hens in the late laying period. In the experiment, 360 Hy-line brown laying hens (67-week-old) were randomly divided into 4 groups with 6 replicates in each group and 15 chickens in each replicate. The control group was fed with basic diets, and EG1, EG2 and EG3 groups were used in 1.5%, 3% and 4.5% BP-fermented feed to replace corn and soybean meal in the basic diet, respectively. The pre-feeding period was 7 days, and the experiment period was 56 days. In terms of production performance, the average daily egg production in the EG3 decreased significantly compared with that in the control group (p < .05). In terms of caecal microorganisms, the abundance indices of Sobs, Chao, and ACE community distribution in EG2 significantly increased compared with those in the control group (p < .05). At the phylum level, the abundance of WPS-2 in all experimental groups significantly increased (p < .05). The abundance of Actinobacteria in EG1 increased significantly, and that of Campilobacterota and Elusimicrobiota in EG2 significantly increased (p < .05). At the genus level, Olsenella in EG1 increased significantly (p<.05) and UCG-008 in EG3 increased significantly (p < .05) with increasing replacement ratio of BP-fermented feed compared with the control group. The results suggest that based on the production performance, the recommended replacement amount is 3%.

This study was conducted to determine the effects of diet supplementation of laying hens with Ligustrum lucidum (LL) on egg production, egg quality and caecal microbiota. A total of 360 72-week-old Hy-Line Brown laying hens were divided into three groups with four replicates of 30 birds each. The laying hens were fed with basal diet (control group), basal diet + 1% LL (group I) and basal diet + 2% LL (group II). The experiment lasted for 45 d. Eggs were collected daily and caecal samples were collected at the end of the experiment. Results showed that dietary supplementation with LL did not affect the average daily egg weight, the average daily feed intake, the cracked egg rate, the mortality and the egg quality (p>.05). However, groups I and II showed significantly increased laying rate and decreased feed/egg ratio (p＜.05). The differences in caecal microbiota between group II and the control group were significant. The relative abundance of Bacteroidetes, Firmicutes, Saccharibacteria and Verrucomicrobia at the phylum level; Ruminococcaceae, Rikenellaceae, Acidaminococcaceae, unclassified_Bacteroidales, S24-7 group and ODP1230B8.23 at the family level; RC9 gut group, Phascolarctobacterium, unclassified_Bacteroidales, Butyricicoccus, norank_f__Ruminococcaceae, Ruminococcaceae_UCG-014, coprostanoligenes group and Ruminococcaceae_UCG-005 at the genus level in group II changed significantly compared with that in the control group (p＜.05). Dietary supplementation with 1% and 2% LL could improve the laying performance and affect the caecal microbial community structure of laying hens during the late laying period.