The previously published structure of the fungal metabolite acremine P is revised by re-evaluation of chemical shift values and NOESY data, and by DFT calculations.

Osteoarthritis is a disease associated with articular cartilage degradation, intra-articular area inflammation, and subchondral bone replacement. Cytokine IL-1β has a prominent function in the inflammations process that passes in the joints. The 70% ethanol extracts of deer antler (250 and 500 mg/kg BW) and glucosamine sulfate (250 kg/BW) were evaluated for four weeks in reducing cytokine IL-1β to rat model OA-induced Monosodium iodoacetate. Measurements of joint diameter in rat’s knee and hyperalgesia were performed on weeks 0, 1, 2, 3, 4, 5, 6, and 7. The presence of a significant difference in the stimulation thermal latency (p = 0.00) and the resulting increase in swelling of joint diameter (p = 0.00) are evidence that MIA has successfully induced the rat modeling of OA. A significant decrease in cytokine IL-Iβ levels was shown on week 3 after MIA injection (p = 0.00). Both concentrations of deer extracts significantly reduced knee joint diameter (p = 0.00), latency thermal stimulation (p = 0.00), and cytokine IL-1β levels (p = 0.00). Based on the results, it can be concluded that the 70% ethanol extract of deer antler is a potential medicine for OA therapy.

This study aimed to obtain a microbial active compound as a novel antimalarial drug from Indonesian isolates. Target-based assays were used to screen for antimalarial activity against the parasite mitochondrial, Plasmodium falciparum malate:quinone oxidoreductase (PfMQO) enzyme. In total, 1600 crude extracts, composed from 800 fungi and 800 actinomycetes extracts, were screened against PfMQO, yielding six active extracts as primary hits. After several stages of stability tests, one extract produced by Aspergillus sp. BioMCC f.T.8501 demonstrated stable PfMQO inhibitory activity. Several purification stages, including OCC, TLC, and HPLC, were performed to obtain bioactive compounds from this active extract. All purification steps were followed by an assay against PfMQO. We identified the active compound as nornidulin based on its LC-MS and UV spectrum data. Nornidulin inhibited PfMQO activity at IC₅₀ of 51 µM and P. falciparum 3D7 proliferation in vitro at IC₅₀ of 44.6 µM, however, it had no effect on the growth of several mammalian cells. In conclusion, we isolated nornidulin from Indonesian Aspergillus sp. BioMCC f.T.8501 as a novel inhibitor of PfMQO, which showed inhibitory activity against the proliferation of P. falciparum 3D7 in vitro.

Pendahuluan: Beberapa spons dari genus Agelas telah dilaporkan memiliki aktivitas antimikroba, namun belum ada laporan tentang aktivitas antimikroba dari Agelas cavernosa. Tujuan: Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol dari spons Agelas cavernosa. Metode: Aktivitas antibakteri pada ekstrak ditentukan dengan menggunakan metode mikrodilusi terhadap Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 dan Pseudomonas aeruginosa ATCC 27853. Pereaksi warna p-iodonitrotetrazolium chloride ditambahkan dalam sumuran untuk memvisualisasikan adanya bakteri hidup. Hasil: ekstrak etanol dari spons A. cavernosa menghambat pertumbuhan ketiga bakteri uji dengan aktivitas antibakteri tertinggi pada Pseudomonas aeruginosa ATCC 27853 dengan MIC 150 ppm. Kesimpulan: Ekstrak ethanol A. cavernosa menunjukkan aktivitas antibakteri.