×Trititrigia cziczinii Tsvel. is a synthetic species obtained as a result of hybridization of different wheat and wheat- grass species. ×T. cziczinii has unique characteristics, as it is a perennial species, with the ability to grow after mowing, high adaptability, resistance to diseases and pests, high protein and gluten content in the grain. All this makes it a promising new crop for agriculture. The new species is a good object for fundamental research in the field of genetics, phylogeny and evolution of cereals (Poaceae). However, there were practically no genetic studies of ×T. cziczinii. The aim of this work was to study the genetic diversity of 24 representatives of two ×T. cziczinii subspecies (ssp. Submitans and ssp. Perenne). To estimate interspecific differences, 17 samples of other tribe Triticeae species (Triticum aestivum, Triticum durum, Agropyron glaucum and Agropyron elon gatum, as well as samples of Triticum-Agropyron and TriticumElymus hybrids) were included in the analysis. For the study, AFLP method (Amplified Fragment Length Polymorphism) was chosen, which allowed us to reveal a sufficiently high polymorphism level of the studied samples. The two primer/enzyme combinations (EcoRI-ACT/MseI-CCC, EcoRI-ACT/MseI-CTA) allow ed the iden tification of 227 fragments, 224 of them were polymorphic (98.68 %), and the level of intraspecific polymorphism of 24 ×T. cziczinii samples was 68.15 %. The iden tified fragments of AFLP spectra, specific for the ×T. cziczinii representatives and the studied wheatgrass species, can be the basis for creating markers that will detect introgressions of genetic material of the genus Agropyron in the T. cziczinii ge nome. Our results indicate a greater genetic relatedness of ×T. cziczinii to T. aestivum than to representatives of the genus Agropyron. According to the cluster analysis, representatives of ×T. cziczinii and varieties of bread wheat were combined into a single subcluster, within which the samples of two species form separate groups. At the same time, the evaluation of the intraspecific genetic diversity of ×T. cziczinii showed no reliable differentiation of representatives of the subspecies Submitans and Perenne, which is probably due to uncertain genetic nature of perenniality, the main feature that divides these subspecies. The study of the unique ×T. cziczinii collection allowed us to obtain the first data on the genetics of the species, while previous studies were focused mainly on phenotypic and economically valuable traits. AFLP analysis used in this study showed high efficiency when working with less studied species, and its results are promising and useful for understanding the genetic structure of the new species (×T. cziczinii Tsvel.).

Relevance. Trititrigia (×Trititrigia cziczinii Tzvel.) this is a new synthetic grain feed crop. The modern collection of the Department of Remote hybridization of the GBS RAS has more than 250 promising samples with a number of economically valuable features. The unique ability of trititrigia in one year to produce a stable yield of high-quality grain (3.0-3.2 t/ha) and nutritious green fodder (27.7-35.1 t/ha) allows us to recommend it for cultivation in regions with unfavorable cultivation conditions for grain crops. Promising samples should be used as donors of valuable traits in breeding for quality and adaptability in order to obtain modern high-quality and adaptive wheat varietiesMaterial and methods. The research was carried out in the Department of Remote Hybridization of the N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences (Moscow region, village of Rozhdestveno) in 2008-2016. The objects of research were: promising long-term and grain-feed lines of trititrigia of the collection of MBG RAS. All samples of trititrigia were obtained in the department of distant hybridization in different years.Results. As a result of the research, it was found that the studied samples are stable lines with a characteristic feature for trititrigia to ripen from top to bottom, the ability to grow in one place for 2- 3 years under favorable conditions, as well as the ability to regenerate after harvesting for grain or mowing for green mass, unique for cultivated cereals. The studied samples have a productive bushi- ness of 8-20 stems per plant. The length of the main ear consistently exceeds the Moscow winter wheat standard 39 and averages 12-13 cm, in which 41 to 97 grains are formed. The grain yield in the competitive variety testing during the research was 3.2-4.1 t/ha, the mass of 1000 grains was 30.1-39.8 g. The Pamyati Lubimovoy variety, selected for a number of economically valuable characteris- tics, passed State registration and became the first trititrigia variety registered in the territory of the Russian Federation.

Многолетняя пшеница — трититригия, несущая гены своих родительских видов, в качестве которых использовали виды пырея Agropyron elongatum (Host) P. Beauv. [syn. Thinopyrum ponticum (Podp.) Zhi W. Liu et R. R.-C. Wang] и A. glaucum (Desf. ex DC) Roem. and Schult. [syn. Thinopyrum intermedium (Host) Barkworth et D. R. Dewey] и сорта мягкой и твердой пшеницы (Triticum aestivum L. и T. durum Desf.) в различных комбинациях, унаследовала много положительных свойств своих родителей (многолетность, способность к отрастанию после уборки, более высокий показатели качества зерна — содержание белка, клейковины и т.д., устойчивость к неблагоприятным почвенно-климатическим условиям, ряду болезней). Для установления объективной оценки качественных показателей зерна трититригии в условиях центрального региона России в зоне рискованного земледелия изучены технологические и хлебопекарные показатели некоторых образцов данной культуры. При обычных условиях для роста и развития растений многолетняя пшеница накапливает в зерне значительное количество белка — 18 – 19 %. Анализ хлебопекарных достоинств трититригии показал, что основные показатели такие как внешний вид, пористость, объем хлеба, превышают показатели сорта озимой пшеницы Московская 39, используемого в качестве стандарта. Исследуемые образцы многолетней пшеницы (М 3202, ЗП 26,548, Памяти Любимовой) имели высокую хлебопекарную оценку — от 3,8 до 4,0 баллов соответственно, тогда как у стандарта Московская 39 общая оценка — 3,7 балла.

The leaf surface micromorphology and the size of the stomatal complex of hybrids in the eighth seed generation from the crossing of ×Trititrigia cziczinii × Elymus farctus (f11814) on the wheat-like wheat-wheatgrass hybrid w107 were investigated by performing scanning electron microscopy of frozen samples (cryoSEM). The micromorphological characteristics of the paternal plants (w107) were dominant in the hybrid leaves. Costal long cells with silicified wavy walls, characteristic of w107 but absent in the mother plants f11814 and E. farctus, were observed in all hybrid samples examined. Conversely, shield-shaped prickles, a characteristic feature of E. farctus, were retained only in some hybrids. In addition, the maternal feature of Ω-shaped junctions of long epidermal cells in the intercostal zone was completely absent in hybrids. Quantitative parameters of the stomatal apparatus showed a weak correlation with micromorphological markers. Stomatal density on the adaxial side was inversely correlated with stomatal size, while variation in these parameters on the abaxial side occurred independently. The prevalence of paternal micromorphological traits in the hybrids seems to be a consequence of the elimination of genetic material from E. farctus, analogous to the loss of chromosomes from wild species observed in other distant crosses.