The rise of Artificial Intelligence (AI) has shown promising performance as a support tool in clinical pathology workflows. In addition to the well-known interobserver variability between dermatopathologists, melanomas present a significant challenge in their histological interpretation. This study aims to analyze all previously published studies on whole-slide images of melanocytic tumors that rely on deep learning techniques for automatic image analysis. Embase, Pubmed, Web of Science, and Virtual Health Library were used to search for relevant studies for the systematic review, in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist. Articles from 2015 to July 2022 were included, with an emphasis placed on the used artificial intelligence methods. Twenty-eight studies that fulfilled the inclusion criteria were grouped into four groups based on their clinical objectives, including pathologists versus deep learning models (n = 10), diagnostic prediction (n = 7); prognosis (n = 5), and histological features (n = 6). These were then analyzed to draw conclusions on the general parameters and conditions of AI in pathology, as well as the necessary factors for better performance in real scenarios.

Spitzoid tumors (ST) are a group of melanocytic tumors of high diagnostic complexity. Since 1948, when Sophie Spitz first described them, the diagnostic uncertainty remains until now, especially in the intermediate category known as Spitz tumor of unknown malignant potential (STUMP) or atypical Spitz tumor. Studies developing deep learning (DL) models to diagnose melanocytic tumors using whole slide imaging (WSI) are scarce, and few used ST for analysis, excluding STUMP. To address this gap, we introduce SOPHIE: the first ST dataset with WSIs, including labels as benign, malignant, and atypical tumors, along with the clinical information of each patient. Additionally, we explain two DL models implemented as validation examples using this database.

Deep learning-based algorithms have led to tremendous progress over the last years, but they face a bottleneck as their optimal development highly relies on access to large datasets. To mitigate this limitation, cross-silo federated learning has emerged as a way to train collaborative models among multiple institutions without having to share the raw data used for model training. However, although artificial intelligence experts have the expertise to develop state-of-the-art models and actively share their code through notebook environments, implementing a federated learning system in real-world applications entails significant engineering and deployment efforts. To reduce the complexity of federation setups and bridge the gap between federated learning and notebook users, this paper introduces a solution that leverages the Jupyter environment as part of the federated learning pipeline and simplifies its automation, the Notebook Federator. The feasibility of this approach is then demonstrated with a collaborative model solving a digital pathology image analysis task in which the federated model reaches an accuracy of 0.8633 on the test set, as compared to the centralized configurations for each institution obtaining 0.7881, 0.6514, and 0.8096, respectively. As a fast and reproducible tool, the proposed solution enables the deployment of a cross-country federated environment in only a few minutes.

Whole-slide imaging (WSI) in cytopathology poses challenges related to segmentation accuracy, computational efficiency, and image acquisition artifacts. This study aims to evaluate the performance of deep-learning models for instance segmentation in cervical cytology, benchmarking them against state-of-the-art methods on both public and institutional datasets. We tested three architectures—U-Net, vision transformer (ViT), and Detectron2—and evaluated their performance on the ISBI 2014 and CNseg datasets using panoptic quality (PQ), dice similarity coefficient (DSC), and intersection over union (IoU). All models were trained on CNseg and tested on an independent institutional dataset. Data preprocessing involved manual annotation using QuPath, patch extraction guided by GeoJSON files, and exclusion of regions containing less than 60% cytologic material. Our models achieved superior segmentation performance on public datasets, reaching up to 98% PQ. Performance decreased on the institutional dataset, likely due to differences in image acquisition and the presence of blurred nuclei. Nevertheless, the models were able to detect blurred nuclei, highlighting their robustness in suboptimal imaging conditions. In conclusion, the proposed models offer an accurate and efficient solution for instance segmentation in cytology WSI. These results support the development of reliable AI-powered tools for digital cytology, with potential applications in automated screening and diagnostic workflows.

Traditionally, Helicobacter pylori (HP) gastritis has been diagnosed by pathologists through the examination of gastric biopsies using optical microscopy with standard hematoxylin and eosin (H&E) staining. However, with the adoption of digital pathology, the identification of HP faces certain limitations, particularly due to insufficient resolution in some scanned images. Moreover, interobserver variability has been well documented in the traditional diagnostic approach, which may further complicate consistent interpretation. In this context, deep convolutional neural network (DCNN) models are showing promising results in the automated detection of this infection in whole-slide images (WSIs). The aim of the present article is to detect the presence of HP infection from our own institutional dataset of histopathological gastric biopsy samples using different pretrained and recognized DCNN and AutoML approaches. The dataset comprises 100 H&E-stained WSIs of gastric biopsies. HP infection was confirmed previously using immunohistochemical confirmation. A total of 45,795 patches were selected for model development. InceptionV3, Resnet50, and VGG16 achieved AUC (area under the curve) values of 1. However, InceptionV3 showed superior metrics such as accuracy (97%), recall (100%), F1 score (97%), and MCC (93%). BoostedNet and AutoKeras achieved accuracy, precision, recall, specificity, and F1 scores less than 85%. The InceptionV3 model was used for external validation, and the predictions across all patches yielded a global accuracy of 78%. In conclusion, DCNN models showed stronger potential for diagnosing HP in gastric biopsies compared with the auto ML approach. However, due to variability across pathology applications, no single model is universally optimal. A problem-specific approach is essential. With growing WSI adoption, DL can improve diagnostic accuracy, reduce variability, and streamline pathology workflows using automation.

Gastrointestinal bleeding in HIV patients secondary to coinfection by HHV8 and development of Kaposi’s sarcoma (KS) is a rare complication even if no skin lesions are detected on physical examination. This article indicates which patients might develop this type of clinical sign and also tries to recall that absence of skin lesions never rules out the presence of KS, especially if gastrointestinal involvement is documented. Gastrointestinal bleeding in terms of hematemesis has rarely been reported in the literature. We review some important clinical findings, diagnosis, and treatment approach. We present the case of an HIV patient who presented to the emergency department with hematemesis and gastrointestinal signs of KS on upper gastrointestinal endoscopy without any dermatological involvement.