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Rabbani Syed
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh,Saudi Arabia

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Journal article
Published: 30 July 2021 in Saudi Journal of Biological Sciences
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Antioxidants are involved in the process of cellular damage prevention, which is considered as an avenue for cancer development. Free radicals are produced in the body upon exposure to stress, cigarette smoke, alcohol, toxins found in personal care products, pesticides in foods, radiation from the sun, viruses, germs or fungi etc. CCND1/CyclinD1 protein was found to be overexpressed in Oral squamous cell carcinoma. One hundred patients with oral squamous cell carcinoma were recruited along with hundred controls for this study from MNJ institute of Oncology with the approval of Ethics Committee, 5ml blood samples were collected from each patient and centrifuged to collect serum for various assays. The antioxidant enzymes like catalase, SOD, GPX and GST were estimated using enzymatic assays. Results were expressed as unit of activity for mg of protein. Insilco analysis is performed using STRING v 11 Protein interaction tool. The patients with oral cancer had significantly reduced activities of SOD, GST and GPX (1.49±0.49, 3.97±0.86 and 10.7±0.73 respectively) compared to healthy controls (4.37±1.43, 6.10±1.12 and 13.8±1.25 respectively) (p<0.005).However no significant difference was observed with regard to catalase activity (2.71± 6.51 and 4.03±1.48) (p=0.28). The proteins interaction PPI enrichment p-value was found to be 3.22e-10 predicted significantly more interactions. Our research findings shown that there was a decline in activity of superoxide dismutase, glutathione peroxidase and glutathione s transferase in addition, personal habits like smoking play a major role in the development and progression of oral carcinogenesis and based on Insilco analysis results CCND1/Cyclin D1 could be the potential therapeutic target in oral squamous cell carcinoma.

ACS Style

P.S. Sushma; Kaiser Jamil; P. Udaykumar; Fahad M. Aldakheel; Shatha A. Alduraywish; Bader Homoud Alali; Ayesha Mateen; Rabbani Syed. Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma. Saudi Journal of Biological Sciences 2021, 1 .

AMA Style

P.S. Sushma, Kaiser Jamil, P. Udaykumar, Fahad M. Aldakheel, Shatha A. Alduraywish, Bader Homoud Alali, Ayesha Mateen, Rabbani Syed. Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma. Saudi Journal of Biological Sciences. 2021; ():1.

Chicago/Turabian Style

P.S. Sushma; Kaiser Jamil; P. Udaykumar; Fahad M. Aldakheel; Shatha A. Alduraywish; Bader Homoud Alali; Ayesha Mateen; Rabbani Syed. 2021. "Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma." Saudi Journal of Biological Sciences , no. : 1.

Journal article
Published: 29 June 2021 in Journal of King Saud University - Science
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Tobacco exposure is the primary leading cause for cancer of the oral cavity, the airways and lung. The carcinogens in cigarette smoke have been linked as an environmental risk factor for causing genetic mutations in tumor suppressor, including TP53 (p53) which has been linked as a causative for occurrence and progression in lung cancer. The aim of the current case-control study is to assess the presence and frequency of chromosomal aberrations between smokers and non-smokers in case and control group. Further, analysis was also extended to include the p53 codon 72 Arg/Pro mutation analysis between the groups of study cohort. In methods, the case-control epidemiological study included a total of 100 cases and controls who were further categorized as smokers and non-smokers for analysis. Chromosomal aberration assay was done on peripheral blood lymphocytes and studied using Geimsa stain, while analysis of the p53 codon 72 Arg/Pro mutation was done by allele-specific polymerase chain reaction assay. The chromosomal aberration assessed include structural abnormalities like breaks, fragment, dysenteric and gaps, as well as numerical aneuploidies. Results showing the number of chromosomal aberrations was higher among smoker cases as compared to controls, and numerical aneuploidy was also detected only among this cohort. The mean of chromosomal aberrations observed between cases and controls and smokers as well as non-smokers was significant at p<0.0001. In case of the p53 genotyping result, the frequency of the Arg/Arg allele was higher among controls as compared to cases and the difference significant at p<0.005. Among female participants, the difference between Arg/Arg and Pro/Arg was also found to be significant between cases and controls. Combined analysis of the p53 codon 72 alleles and mean of chromosomal aberrations between cases and controls was found to be significant at p<0.005.Our study found an association between risk of lung cancer as well as occurrence of chromosomal aberrations. In case of the p53 polymorphism, the significance was detected only among females. In Conclusion, the studied p53 variant and chromosomal aberration along with smoking has shown significant association with increased risk of lung cancer.

ACS Style

Fahad M. Aldakheel; Abdulwahab A. Abuderman; Bader Homoud Alali; Ayesha Mateen; Shatha A. Alduraywish; Kaiser Jamil; Mohammed S. Alqahtani; Rabbani Syed. Smoking and P53 polymorphism association with chromosomal aberration in lung cancer. Journal of King Saud University - Science 2021, 33, 101533 .

AMA Style

Fahad M. Aldakheel, Abdulwahab A. Abuderman, Bader Homoud Alali, Ayesha Mateen, Shatha A. Alduraywish, Kaiser Jamil, Mohammed S. Alqahtani, Rabbani Syed. Smoking and P53 polymorphism association with chromosomal aberration in lung cancer. Journal of King Saud University - Science. 2021; 33 (6):101533.

Chicago/Turabian Style

Fahad M. Aldakheel; Abdulwahab A. Abuderman; Bader Homoud Alali; Ayesha Mateen; Shatha A. Alduraywish; Kaiser Jamil; Mohammed S. Alqahtani; Rabbani Syed. 2021. "Smoking and P53 polymorphism association with chromosomal aberration in lung cancer." Journal of King Saud University - Science 33, no. 6: 101533.

Research article
Published: 09 April 2021 in Luminescence
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Four Ag(I) complexes; 1.PF6, 2.PF6, 1.ClO4 and 2.ClO4 of bis (methyl)thia salen (1) and bis (methyl)selena salen (2) with two different counter anions (PF6‐ and ClO4‐) have been investigated for DNA binding properties. In vitro interactional association between the Ag(I) complexes and ct‐DNA has been examined by performing the spectroscopic titrations on absorption spectrophotometer and fluorescence spectrophotometer. Competitive binding study has also been done by a fluorescence spectrophotometer using ethidium bromide as a classical intercalator. The spectroscopic methods revealed a major groove. Viscometry and Agarose gel electrophoresis experiments have also been performed as physicochemical methods to confirm the binding of complex molecules with DNA. Molecular docking analysis has been executed to get the theoretical insight into the mode of binding. The docking study demonstrated the major groove binding of all four complexes to the DNA with electrostatic metal‐phosphate interactions (between the metal and DNA’s backbone) and hydrophobic interactions. Cytotoxicity of the complexes has been studied on the Human Fibroblast foreskin (HFF) cell line. The cytotoxicity results showed positive gesture for moving ahead in the next level of screening; the values were above 10 μM appreciated for the normal cell lines.

ACS Style

Mamta Tripathi; Rabbani Syed; Antony Stalin; Abdul Malik; Rama Pande; Ashish Kumar Asatkar. In vitro investigation of biophysical interactions between Ag(I) complexes of bis(methyl)(thia/selena)salen and ct‐DNA via multi‐spectroscopic, physicochemical and molecular docking methods along with cytotoxicity study. Luminescence 2021, 1 .

AMA Style

Mamta Tripathi, Rabbani Syed, Antony Stalin, Abdul Malik, Rama Pande, Ashish Kumar Asatkar. In vitro investigation of biophysical interactions between Ag(I) complexes of bis(methyl)(thia/selena)salen and ct‐DNA via multi‐spectroscopic, physicochemical and molecular docking methods along with cytotoxicity study. Luminescence. 2021; ():1.

Chicago/Turabian Style

Mamta Tripathi; Rabbani Syed; Antony Stalin; Abdul Malik; Rama Pande; Ashish Kumar Asatkar. 2021. "In vitro investigation of biophysical interactions between Ag(I) complexes of bis(methyl)(thia/selena)salen and ct‐DNA via multi‐spectroscopic, physicochemical and molecular docking methods along with cytotoxicity study." Luminescence , no. : 1.

Journal article
Published: 28 January 2021 in Journal of Infection and Public Health
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G1896A switch is one of the hotspots in subjects affected with hepatitis B. This hotspot mutation is observed in all the different spectrum of hepatitis B, and it has a very dangerous and a long lasting effect. The major purpose of the study was to screen G1986A mutations at a large scale and also to establish ligase chain reaction as a mutation testing tool. Polymerase chain reaction (PCR) and Nucleotide Sequencing was done to identify the G1896A mutation in the precore region of the genome. All the 331 HBV positive patients were screened. Almost 29% (24/82) of the cases remarkably had the presence of G1896A mutation confirmed by LCR and direct sequencing. The precore G1896A mutation is responsible for one third of the patients suffering from precore stop codon mutation. It clearly exhibits that LCR is 100% in sync with direct sequencing and is extremely reliable and the results are highly reproducible.

ACS Style

Abdul Malik; Fahad Aldakheel; Syed Rabbani; Meshal Alshehri; Anis Ahmad Chaudhary; Musaed Alkholief; Aws Alshamsan. LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B. Journal of Infection and Public Health 2021, 14, 651 -654.

AMA Style

Abdul Malik, Fahad Aldakheel, Syed Rabbani, Meshal Alshehri, Anis Ahmad Chaudhary, Musaed Alkholief, Aws Alshamsan. LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B. Journal of Infection and Public Health. 2021; 14 (5):651-654.

Chicago/Turabian Style

Abdul Malik; Fahad Aldakheel; Syed Rabbani; Meshal Alshehri; Anis Ahmad Chaudhary; Musaed Alkholief; Aws Alshamsan. 2021. "LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B." Journal of Infection and Public Health 14, no. 5: 651-654.

Journal article
Published: 02 November 2020 in Polymers
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The main objective of the present study was to investigate the hemo and immune compatibility of gliadin nanoparticles as a function of particle size. Gliadin nanoparticles of different size were prepared using a modified antisolvent nanoprecipitation method. The hemolytic potential of gliadin nanoparticles was evaluated using in vitro hemolysis assay. Phagocytic uptake of gliadin nanoparticles was studied using rat polymorphonuclear (PMN) leukocytes and murine alveolar peritoneal macrophage (J774) cells. In vivo immunogenicity of gliadin nanoparticles was studied following subcutaneous administration in mice. Gliadin nanoparticles were non-hemolytic irrespective of particle size and hence compatible with blood components. In comparison to positive control zymosan, gliadin nanoparticles with a size greater than 406 ± 11 nm showed higher phagocytic uptake in PMN cells, while the uptake was minimal with smaller nanoparticles (127 ± 8 nm). Similar uptake of gliadin nanoparticles was observed in murine alveolar peritoneal macrophages. Anti-gliadin IgG antibody titers subsequent to primary and secondary immunization of gliadin nanoparticles in mice were in the increasing order of 406 ± 11 nm < 848 ± 20 nm < coarse suspension). On the other hand, gliadin nanoparticles of 127 ± 8 nm in size did not elicit immunogenic response. Phagocytosis and immunogenicity of gliadin nanoparticles are strongly influenced by particle size. The results of this study can provide useful information for rational design of protein-based nanomaterials in drug delivery applications.

ACS Style

Mohammed Alqahtani; Rabbani Syed; Meshal Alshehri. Size-Dependent Phagocytic Uptake and Immunogenicity of Gliadin Nanoparticles. Polymers 2020, 12, 2576 .

AMA Style

Mohammed Alqahtani, Rabbani Syed, Meshal Alshehri. Size-Dependent Phagocytic Uptake and Immunogenicity of Gliadin Nanoparticles. Polymers. 2020; 12 (11):2576.

Chicago/Turabian Style

Mohammed Alqahtani; Rabbani Syed; Meshal Alshehri. 2020. "Size-Dependent Phagocytic Uptake and Immunogenicity of Gliadin Nanoparticles." Polymers 12, no. 11: 2576.

Journal article
Published: 29 April 2020 in Processes
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Bee pollens are rich source of essential amino acids and are often considered as complete food for human beings. Herein, we exploited the potential reducing abilities of Bee pollens extract for the eco-friendly preparation of silver nanoparticles (AgNPs-G). The resulting NPs were characterized using a combination of microscopic and spectroscopic techniques. The analyses confirm the formation of spherical Ag NPs. AgNPs-G obtained from the aqueous extract of bee pollens was used to study their antibacterial properties against Gram-positive and Gram-negative microbes using the Minimum Inhibitory Concentration 50 (MIC50) method. The antibacterial properties of AgNPs-G were compared to the properties of chemically synthesized Ag NPs (AgNPs-C) using sodium borohydride as a reducing agent. The green synthesized nanoparticles (AgNPs-G) exhibited a better antibacterial activity against most of the studied strains when compared to the chemically synthesized Ag NPs (AgNPs-C). In addition, the anti-cancer activity of Ag NPs was also studied against human liver and breast carcinoma cell lines by applying MTT-assay. The Ag NPs demonstrated considerable anticancer activity against the studied cell lines and exhibited high IC50 values in both MCF-7 and HepG2 cell lines.

ACS Style

Hanan M. Al-Yousef; Musarat Amina; Ali S. Alqahtani; Mohammed S. Alqahtani; Abdul Malik; Mohammad Rafe Hatshan; Mohammed Rafiq H. Siddiqui; Mujeeb Khan; Mohammed Rafi Shaik; Mohammad Shamsul Ola; Rabbani Syed. Pollen Bee Aqueous Extract-Based Synthesis of Silver Nanoparticles and Evaluation of Their Anti-Cancer and Anti-Bacterial Activities. Processes 2020, 8, 524 .

AMA Style

Hanan M. Al-Yousef, Musarat Amina, Ali S. Alqahtani, Mohammed S. Alqahtani, Abdul Malik, Mohammad Rafe Hatshan, Mohammed Rafiq H. Siddiqui, Mujeeb Khan, Mohammed Rafi Shaik, Mohammad Shamsul Ola, Rabbani Syed. Pollen Bee Aqueous Extract-Based Synthesis of Silver Nanoparticles and Evaluation of Their Anti-Cancer and Anti-Bacterial Activities. Processes. 2020; 8 (5):524.

Chicago/Turabian Style

Hanan M. Al-Yousef; Musarat Amina; Ali S. Alqahtani; Mohammed S. Alqahtani; Abdul Malik; Mohammad Rafe Hatshan; Mohammed Rafiq H. Siddiqui; Mujeeb Khan; Mohammed Rafi Shaik; Mohammad Shamsul Ola; Rabbani Syed. 2020. "Pollen Bee Aqueous Extract-Based Synthesis of Silver Nanoparticles and Evaluation of Their Anti-Cancer and Anti-Bacterial Activities." Processes 8, no. 5: 524.

Chapter
Published: 29 March 2020 in Model Organisms for Microbial Pathogenesis, Biofilm Formation and Antimicrobial Drug Discovery
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Biofilms are bacterial cells, especially planktonic bacteria, structured as a community; they group, grow, and develop together by way of complex chemical and molecular communication. The biochemical communication in a biofilm is termed “quorum sensing,” and constitutes the most vital component of their structural and functional stability. In addition to this constant chemical communication, they also exchange nutrients and sometimes genetic components, giving rise to daughter cells with altered genetic makeup. Bacterial biofilms can occur in different contexts, including dental plaque on teeth and pond scum in the environment. Bacterial biofilms are usually infectious and pose considerable challenges to treat. After sufficient growth and expansion, biofilms start discharging their “virulence” in either of two forms: (1) planktonic shower or (2) fragment detachment. The huge mass of bacterial cells in a biofilm is immensely stronger than the corresponding singular planktonic pattern, and their collective virulence presents a heavy challenge to the host’s immune cells. Biofilm bacteria are usually resistant to most common antibiotics, and present a great threat of ruinous infection. Infectious biofilms can sometimes also demand surgical resection of the infected tissue or organ. In addition to the bacterial biofilms, fungi and viral particles integrate to form large communities, producing concomitantly challenging infections. Many species of fungi including Aspergillus, Cryptococcus, Trichosporon, and Pneumocystis are all capable of forming fungal biofilms and collectively exhibit resistance to antifungal therapies. However, biofilms are not always menacing to human life. Their beneficial effects and biotechnological applications are very active fields of research. Nonetheless, biofilms of bacterial, fungal, and viral origin all constitute major platforms for chronic infections and diseases. As a mechanistic approach, scientists believe that the destruction or inhibition of “quorum sensing” can enable disruption of bacterial biofilms. Research also supports that pyruvate is essential for the construction and maintenance of biofilms, and its depletion can degrade their strength. This chapter will explain in detail the molecular signaling, construction, and maintenance of biofilms, their potential threats and beneficial applications, and current research goals.

ACS Style

Rabbani Syed; Mohammed Alqahtani; Ayesha Mateen; Fawaz Alasmari; Youssef Sari. Understanding Biofilm Dynamics: In Vitro and In Vivo Models. Model Organisms for Microbial Pathogenesis, Biofilm Formation and Antimicrobial Drug Discovery 2020, 509 -526.

AMA Style

Rabbani Syed, Mohammed Alqahtani, Ayesha Mateen, Fawaz Alasmari, Youssef Sari. Understanding Biofilm Dynamics: In Vitro and In Vivo Models. Model Organisms for Microbial Pathogenesis, Biofilm Formation and Antimicrobial Drug Discovery. 2020; ():509-526.

Chicago/Turabian Style

Rabbani Syed; Mohammed Alqahtani; Ayesha Mateen; Fawaz Alasmari; Youssef Sari. 2020. "Understanding Biofilm Dynamics: In Vitro and In Vivo Models." Model Organisms for Microbial Pathogenesis, Biofilm Formation and Antimicrobial Drug Discovery , no. : 509-526.

Journal article
Published: 18 November 2019 in Processes
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In this current study, we demonstrated the green synthesis and characterization of silver nanoparticles using Myrtus communis L. plant extract (Ag-MC) and its evaluation of anticancer and antimicrobial activities. The green synthesis of (Ag-MC), was assessed by numerous characterization techniques such as ultraviolet-visible spectroscopy (UV-VIS), Fourier-transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD) transmission electron microscopy (TEM) and energy dispersive x-ray spectroscopy (EDX). The anti-cancer activity of the green synthesized silver nanoparticles was evaluated by the median inhibitory dose (IC50) on human liver carcinoma cell lines (HepG2). These results suggested that SN-NPs can be used as effective anticancer cell lines, as well as antibacterial and antiseptic agents in the medical field. This study showed that overexpression of aldose reductase (AR) in the human liver carcinoma cell line, HepG2, was down regulated by administration of SN-MC. The down regulation of AR was associated with abrogation of Pl3k/Akt, ERK and NF-kB pathways and the inhibition of cancer hallmarks, however, the target molecule for SN-MC was not practically established. Thus it is still unknown if the consequences were due to AR inhibition or direct Ag-MC interaction with AR.

ACS Style

Abdulwahab Ali Abuderman; Rabbani Syed; Abdullah A. Alyousef; Mohammed S. Alqahtani; Mohammad Shamsul Ola; Abdul Malik. Green Synthesized Silver Nanoparticles of Myrtus communis L (AgMC) Extract Inhibits Cancer Hallmarks via Targeting Aldose Reductase (AR) and Associated Signaling Network. Processes 2019, 7, 860 .

AMA Style

Abdulwahab Ali Abuderman, Rabbani Syed, Abdullah A. Alyousef, Mohammed S. Alqahtani, Mohammad Shamsul Ola, Abdul Malik. Green Synthesized Silver Nanoparticles of Myrtus communis L (AgMC) Extract Inhibits Cancer Hallmarks via Targeting Aldose Reductase (AR) and Associated Signaling Network. Processes. 2019; 7 (11):860.

Chicago/Turabian Style

Abdulwahab Ali Abuderman; Rabbani Syed; Abdullah A. Alyousef; Mohammed S. Alqahtani; Mohammad Shamsul Ola; Abdul Malik. 2019. "Green Synthesized Silver Nanoparticles of Myrtus communis L (AgMC) Extract Inhibits Cancer Hallmarks via Targeting Aldose Reductase (AR) and Associated Signaling Network." Processes 7, no. 11: 860.

Journal article
Published: 01 July 2019 in Journal of AOAC INTERNATIONAL
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Background: Alanine-rich proteins/peptides (ARP), with bioactivity of up to 20 amino acid residues, can be observed by the body easily during gastrointestinal digestion. Objective: Populus trichocarpa extract’s capability to attenuate quorum sensing-regulated virulence and biofilm formation in Staphylococcus aureus is described. Methods: PT13, an ARP obtained from P. trichocarpa, was tested for its activity against S. aureus using the broth microdilution test; a crystal-violet biofilm assay was performed under a scanning electron microscope. The production of various virulence factors was estimated with PT13 treatment. Microarray gene expression profiling of PT13-treated S. aureus was conducted and compared with an untreated control. Exopolysaccharides (EPS) was estimated to observe the PT13 inhibition activity. Results: PT13 was antimicrobial toward S. aureus at different concentrations and showed a similar growth rate in the presence and absence of PT13 at concentrations ≤8 μg/mL. Biofilm production was interrupted even at low concentrations, and biofilm-related genes were down-regulated when exposed to PT13. The genes encoding cell adhesion and bacterial attachment protein were the major genes suppressed by PT13. In addition, hemolysins, clumping activity, and EPS production of S. aureus decreased after treatment in a concentration-dependent manner. Conclusions: A long-chain PT13 with effective actions that, even at low concentration levels, not only regulated the gene expression in the producer organism but also blocked the virulence gene expression in this Gram-positive human pathogen is described. Highlights: We identified a PT13 as a potential antivirulence agent that regulated production of bacterial virulence determinants (e.g., toxins, enzymes and biofilm), downwards and it may be a promising anti-virulence agent to be further developed as an anti-infective agent.

ACS Style

Raid Al Akeel; Ayesha Mateen; Rabbani Syed. An Alanine-Rich Peptide Attenuates Quorum Sensing-Regulated Virulence and Biofilm Formation in Staphylococcus aureus. Journal of AOAC INTERNATIONAL 2019, 102, 1228 -1234.

AMA Style

Raid Al Akeel, Ayesha Mateen, Rabbani Syed. An Alanine-Rich Peptide Attenuates Quorum Sensing-Regulated Virulence and Biofilm Formation in Staphylococcus aureus. Journal of AOAC INTERNATIONAL. 2019; 102 (4):1228-1234.

Chicago/Turabian Style

Raid Al Akeel; Ayesha Mateen; Rabbani Syed. 2019. "An Alanine-Rich Peptide Attenuates Quorum Sensing-Regulated Virulence and Biofilm Formation in Staphylococcus aureus." Journal of AOAC INTERNATIONAL 102, no. 4: 1228-1234.

Original article
Published: 27 June 2019 in Molecular Biology Reports
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From the literature review, there seem to be no studies conducted on infection caused by Helicobacter pylori in patients with gastric MALT lymphoma in the KSA region. The present research is an attempt to understand the prevalence of patients infected with H. pylori in the selected region and the role of allelic imbalance of chromosome 3p regions to understand the clinical manifestations and features associated with MALT lymphomagenesis. The researcher analyzed the frequency of infection in patients from the region of Saudi Arabia by examining the data collected from hospitals and biopsy tissue samples as per the recommended protocol. The endoscopic diagnosis was performed to collect biopsy samples. Histology and AP-PCR DNA fingerprinting analyses were performed from the endoscopic gastric mucosal biopsies collected from patients with associated gastric MALT lymphoma. The existence of H. pylori was examined based on the results of gastric mucosal biopsies stained with hematoxylin-eosin (H&E) and Steiner's silver stains. MALT, MALT lymphoma tissue samples and H. pylori-positive chronic gastritis were examined for LOH at chromosome 3p24 using standard procedures and techniques. The findings of the paper revealed the H. pylori was found to be positive in 17% of the cases significantly high among the age group of 31-50 years. Patients with MALT, MALT lymphoma, and H. pylori-associated gastritis presented features such as lymphocyte accumulation, vacuolation, Peyer's patch appearance, and lymphatic follicles. H. pylori were found to appear as a dense colored accumulated mass in the gastric epithelial layer. The findings from AP-PCR generated DNA fingerprints revealed intense band including two prominent bands in MALT lymphoma. Among other loci, 3p24 was the only one locus that showed high percentages of LOH as reported earlier in all cancer-related cases. The findings of this research paper empower the fact that allelic imbalances play a vital role in the development of MALT lymphoma. However, future researches should be conducted to identify the chromosome regions of the AP-PCR generated DNA fingerprints of human gastric MALT lymphoma in order to confirm this proposition.

ACS Style

Abdul Wahab Ali Abuderman; Rabbani Syed; Ayesha Mateen; Abdul Malik; Mohammad Ola. Epidemiological characterization, genetic alterations of Helicobacter pylori infection in chronic gastric disorder and prognostic values of heterozygosity loss in chromosome 3p. Molecular Biology Reports 2019, 46, 4323 -4332.

AMA Style

Abdul Wahab Ali Abuderman, Rabbani Syed, Ayesha Mateen, Abdul Malik, Mohammad Ola. Epidemiological characterization, genetic alterations of Helicobacter pylori infection in chronic gastric disorder and prognostic values of heterozygosity loss in chromosome 3p. Molecular Biology Reports. 2019; 46 (4):4323-4332.

Chicago/Turabian Style

Abdul Wahab Ali Abuderman; Rabbani Syed; Ayesha Mateen; Abdul Malik; Mohammad Ola. 2019. "Epidemiological characterization, genetic alterations of Helicobacter pylori infection in chronic gastric disorder and prognostic values of heterozygosity loss in chromosome 3p." Molecular Biology Reports 46, no. 4: 4323-4332.

Paper
Published: 17 June 2019 in Journal of Materials Chemistry B
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Lignin nanoparticles (LNPs) were prepared with the objective of evaluating their application as a novel oral drug delivery system.

ACS Style

Mohammed S. Alqahtani; Ali Alqahtani; Abdullah Al-Thabit; Monzurul Roni; Rabbani Syed. Novel lignin nanoparticles for oral drug delivery. Journal of Materials Chemistry B 2019, 7, 4461 -4473.

AMA Style

Mohammed S. Alqahtani, Ali Alqahtani, Abdullah Al-Thabit, Monzurul Roni, Rabbani Syed. Novel lignin nanoparticles for oral drug delivery. Journal of Materials Chemistry B. 2019; 7 (28):4461-4473.

Chicago/Turabian Style

Mohammed S. Alqahtani; Ali Alqahtani; Abdullah Al-Thabit; Monzurul Roni; Rabbani Syed. 2019. "Novel lignin nanoparticles for oral drug delivery." Journal of Materials Chemistry B 7, no. 28: 4461-4473.

Journal article
Published: 07 December 2018 in Saudi Journal of Biological Sciences
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Fennel (Foeniculum vulgare Mill.) member from the family Umbelliferae (Apiaceae) and has been used in Saudi Arabia as an medicine as of the from the tradition. Our previous work with seed extracts of this plant generated DEAE-ion exchange purified proteins that exhibited antibacterial properties. The current study moves this work forward by using 2-D gel separation and MALDI TOF/TOF to identify proteins in this active extract. Fourteen protein spots were excised, digested, and identified. Several putative functions were identified, including: a copper-trans locating ATPase PAA1 chloroplastic-like isoform X1; a cytosolic enolase; a putative pentatricopeptide repeat-containing protein; an NADP-requiring isocitrate dehydrogenase; two proteins annotated as being encoded downstream from Son-like proteins; three probable nuclear proteins 5-1; and four predicted/ unidentified proteins. Future efforts will further characterize their relevant antimicrobial properties with the aim of cloning and high throughput synthesis of the antimicrobial element(s).

ACS Style

Abdullah A. Alyousef; Ayesha Mateen; Raid Al –Akeel; Abdulaziz Alqasim; Yazeed Al –Sheikh; Mohammed Alqahtani; Rabbani Syed. Screening & analysis of anionic peptides from Foeniculum vulgare Mill by mass spectroscopy. Saudi Journal of Biological Sciences 2018, 26, 660 -664.

AMA Style

Abdullah A. Alyousef, Ayesha Mateen, Raid Al –Akeel, Abdulaziz Alqasim, Yazeed Al –Sheikh, Mohammed Alqahtani, Rabbani Syed. Screening & analysis of anionic peptides from Foeniculum vulgare Mill by mass spectroscopy. Saudi Journal of Biological Sciences. 2018; 26 (4):660-664.

Chicago/Turabian Style

Abdullah A. Alyousef; Ayesha Mateen; Raid Al –Akeel; Abdulaziz Alqasim; Yazeed Al –Sheikh; Mohammed Alqahtani; Rabbani Syed. 2018. "Screening & analysis of anionic peptides from Foeniculum vulgare Mill by mass spectroscopy." Saudi Journal of Biological Sciences 26, no. 4: 660-664.

Journal article
Published: 01 August 2018 in Microbial Pathogenesis
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Due to growing concern towards microbial resistance, ongoing search for developing novel bioactive compounds such as peptides is on rise. The aim of this study was to evaluate antimicrobial effect of Populus trichocarpa extract, chemically identify the active peptide fraction and finds its target in Staphylococcus aureus. In this study the active fraction of P. trichocarpa crude extract was purified and characterized using MS/MS. This peptide PT13 antimicrobial activity was confirmed by in-vitro agar based disk diffusion and in-vivo infection model of G. mellonella. The proteomic expression analysis of S. aureus under influence of PT13 was studied using LTQ-Orbitrap-MS in-solution digestion and identity of target protein was acquired with their quantified expression using label-free approach of Progenesis QI software. Docking study was performed with peptide PT13 and its target YycG protein using CABS-dock. The active fraction PT13 sequence was identified as KVPVAAAAAAAAAVVASSMVVAAAK, with 25 amino acid including 13 alanine having M/Z 2194.2469. PT13 was uniformly inhibited growth S. aureus SA91 and MIC was determined 16 μg/mL for SA91 S. aureus strain. Sensor histidine kinase (YycG) was most significant target found differentially expressed under influence of PT13. G. mellonella larvae were killed rapidly due to S aureus infection, whereas death in protected group was insignificant in compare to control. The docking models showed ten docking models with RMSD value 1.89 for cluster 1 and RMSD value 3.95 for cluster 2 which is predicted to be high quality model. Alanine rich peptide could be useful in constructing as antimicrobial peptide for targeting extracellular Domain of Sensor Histidine Kinase YycG from S. aureus used in the study.

ACS Style

Raid Al Akeel; Ayesha Mateen; Rabbani Syed; Mohammed Alqahtani; Ali S. Alqahtani. Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein. Microbial Pathogenesis 2018, 121, 115 -122.

AMA Style

Raid Al Akeel, Ayesha Mateen, Rabbani Syed, Mohammed Alqahtani, Ali S. Alqahtani. Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein. Microbial Pathogenesis. 2018; 121 ():115-122.

Chicago/Turabian Style

Raid Al Akeel; Ayesha Mateen; Rabbani Syed; Mohammed Alqahtani; Ali S. Alqahtani. 2018. "Alanine rich peptide from Populus trichocarpa inhibit growth of Staphylococcus aureus via targetting its extracellular domain of Sensor Histidine Kinase YycGex protein." Microbial Pathogenesis 121, no. : 115-122.

Journal article
Published: 03 April 2018 in BMC Complementary and Alternative Medicine
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Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consider its potential antibacterial action. Extracts were purified by dialysis and ion exchange chromatography strategy and then assayed for antibacterial activity against four standard pathogenic bacterial strains known to cause foodborne infections and spoilage of food and herbal drugs. Antimicrobial peptides were extracted from seeds using a sodium phosphate citrate (pH 7.2) - CTAB cradle (pH 6.0). The highest protein concentration was seen with elute fractions 1 and 3 (370 mg/mL) compared with elute fractions 2 and 4 (340 mg/mL). Among the bacteria utilized, E. coli was clearly the most sensitive out of selected four strains. Our results suggest that Cucumis sativus L seeds extracts have significant potentials as new antimicrobial agents.

ACS Style

Raid Al Akeel; Ayesha Mateen; Khalid K. Alharbi; Abdullah A. Alyousef; Hazem M. Al-Mandeel; Rabbani Syed. Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds. BMC Complementary and Alternative Medicine 2018, 18, 121 .

AMA Style

Raid Al Akeel, Ayesha Mateen, Khalid K. Alharbi, Abdullah A. Alyousef, Hazem M. Al-Mandeel, Rabbani Syed. Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds. BMC Complementary and Alternative Medicine. 2018; 18 (1):121.

Chicago/Turabian Style

Raid Al Akeel; Ayesha Mateen; Khalid K. Alharbi; Abdullah A. Alyousef; Hazem M. Al-Mandeel; Rabbani Syed. 2018. "Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds." BMC Complementary and Alternative Medicine 18, no. 1: 121.

Journal article
Published: 01 January 2017 in Genetic Testing and Molecular Biomarkers
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Background: Obesity is known to be a complex disorder caused by both genetic and environmental factors. Patients with obesity tend to develop cardiovascular disease and type 2 diabetes. Previous studies have revealed that obesity is associated with genetic variations including those found in the apolipoprotein E (APOE) gene, which also affects lipid profiles. Hence, in this study, we aimed to perform a molecular characterization of APOE gene polymorphisms found in overweight subjects within a Saudi population. Methods: A case–control study was performed consisting of 198 cases and 198 controls, selected from participants at the King Saud University. TaqMan genotyping was performed to characterize the APOE gene polymorphisms. Results: The present study identified the E4 allele of the APOE gene as being significantly associated with obesity in the Saudi population (p = 0.0001). We found a statistically significant difference in the genotype distribution between cases and controls [for E3/E4: OR, 2.16 (95% CI: 1.19–3.91); p = 0.009]. Discussion: Significant differences were observed in the APOE allele profiles (p < 0.001) and lipid profile parameters, including triglycerides and low-density lipoprotein among the obese patients compared with the non-obese control population. Conclusion: Our results confirm that APOE variants are associated with obesity in the Saudi population.

ACS Style

Khalid Khalaf Alharbi; Rabbani Syed; Fawiziah Khalaf Alharbi; Imran Ali Khan. Association of Apolipoprotein E Polymorphism with Impact on Overweight University Pupils. Genetic Testing and Molecular Biomarkers 2017, 21, 53 -57.

AMA Style

Khalid Khalaf Alharbi, Rabbani Syed, Fawiziah Khalaf Alharbi, Imran Ali Khan. Association of Apolipoprotein E Polymorphism with Impact on Overweight University Pupils. Genetic Testing and Molecular Biomarkers. 2017; 21 (1):53-57.

Chicago/Turabian Style

Khalid Khalaf Alharbi; Rabbani Syed; Fawiziah Khalaf Alharbi; Imran Ali Khan. 2017. "Association of Apolipoprotein E Polymorphism with Impact on Overweight University Pupils." Genetic Testing and Molecular Biomarkers 21, no. 1: 53-57.

Review
Published: 18 March 2015 in Asian Pacific Journal of Cancer Prevention
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Clinical Efficacy and Possible Applications of Genomics in Lung Cancer Lung cancer;genomics;personalized treatment; The heterogeneous nature of lung cancer has become increasingly apparent since introduction of molecular classification. In general, advanced lung cancer is an aggressive malignancy with a poor prognosis. Activating alterations in several potential driver oncogenic genes have been identified, including EGFR, ROS1 and ALK and understanding of their molecular mechanisms underlying development, progression, and survival of lung cancer has led to the design of personalized treatments that have produced superior clinical outcomes in tumours harbouring these mutations. In light of the tsunami of new biomarkers and targeted agents, next generation sequencing testing strategies will be more appropriate in identifying the patients for each therapy and enabling personalized patients care. The challenge now is how best to interpret the results of these genomic tests, in the context of other clinical data, to optimize treatment choices. In genomic era of cancer treatment, the traditional one-size-fits-all paradigm is being replaced with more effective, personalized oncologic care. This review provides an overview of lung cancer genomics and personalized treatment.

ACS Style

Khalid Khalaf Alharbi. Clinical Efficacy and Possible Applications of Genomics in Lung Cancer. Asian Pacific Journal of Cancer Prevention 2015, 16, 1693 -1698.

AMA Style

Khalid Khalaf Alharbi. Clinical Efficacy and Possible Applications of Genomics in Lung Cancer. Asian Pacific Journal of Cancer Prevention. 2015; 16 (5):1693-1698.

Chicago/Turabian Style

Khalid Khalaf Alharbi. 2015. "Clinical Efficacy and Possible Applications of Genomics in Lung Cancer." Asian Pacific Journal of Cancer Prevention 16, no. 5: 1693-1698.

Journal article
Published: 01 January 2014 in Lipids in Health and Disease
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Fatty acid-binding protein 2 (FABP2) is an intracellular protein expressed exclusively in the enterocytes of proximal small intestine. FABP2 has a high affinity for saturated and unsaturated long-chain fatty acids and is believed to be involved in the absorption and transport of dietary fatty acids. This is a case–control study conceded in 438 T2DM cases and 460 subjects with normal glucose levels and non-obese considered as healthy controls. Allelic discrimination was performed using TaqMan single-nucleotide polymorphism was carried out by real time-polymerase chain reaction (RT-PCR) assays using purified DNA. Clinical data and anthropometric measurements except age, glucose levels and lipid profile of the patients were significantly different from those of the controls (p < 0.05). Statistical analyses failed to show any type of significant association of the polymorphism between cases and controls. However logistic regression analyses was suggests that the TT genotype is significantly associated with male patients (p = 0.001). None of the allele or genotypes of FABP2 A54T was associated with T2DM cases versus the controls (AT genotype, OR = 0.85 (0.64-1.12), p = 0.25; TT genotype, OR = 0.66 (0.39-1.11), p = 0.11; T allele, 0.82 (0.67-1.02), p = 0.08). In conclusion, this study suggests that the above named variant in FABP2 gene is not potential contributor to the risk of T2DM and related traits in a Saudi population. However TT genotype is a risk factor for the disease in males.

ACS Style

Khalid Khalaf Alharbi; Imran Ali Khan; Mohammad D Bazzi; Nasser M Al-Daghri; Tarique N Hasan; May Salem Alnbaheen; Fawiziah Khalaf Alharbi; Yazeed A Al-Sheikh; Rabbani Syed; Mourad Am Aboul-Soud. A54T polymorphism in the fatty acid binding protein 2 studies in a Saudi population with type 2 diabetes mellitus. Lipids in Health and Disease 2014, 13, 61 -61.

AMA Style

Khalid Khalaf Alharbi, Imran Ali Khan, Mohammad D Bazzi, Nasser M Al-Daghri, Tarique N Hasan, May Salem Alnbaheen, Fawiziah Khalaf Alharbi, Yazeed A Al-Sheikh, Rabbani Syed, Mourad Am Aboul-Soud. A54T polymorphism in the fatty acid binding protein 2 studies in a Saudi population with type 2 diabetes mellitus. Lipids in Health and Disease. 2014; 13 (1):61-61.

Chicago/Turabian Style

Khalid Khalaf Alharbi; Imran Ali Khan; Mohammad D Bazzi; Nasser M Al-Daghri; Tarique N Hasan; May Salem Alnbaheen; Fawiziah Khalaf Alharbi; Yazeed A Al-Sheikh; Rabbani Syed; Mourad Am Aboul-Soud. 2014. "A54T polymorphism in the fatty acid binding protein 2 studies in a Saudi population with type 2 diabetes mellitus." Lipids in Health and Disease 13, no. 1: 61-61.