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Ali S. Alqahtani
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

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Journal article
Published: 09 August 2021 in Saudi Pharmaceutical Journal
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Elevation in hyperglycemia-associated methylglyoxal level can trigger vascular endothelial cells oxidative stress and apoptosis. The present work assesses the cell proliferative, anti-oxidative and anti-apoptotic potential of Suaeda monoica derived four new terpenes: a norsesquaterpenol (normonisesquaterpenol), a monocyclic triterpenoid (suaedanortriterpene dione), an aromatic monoterpenic ester and a labdane-type norditerpenic xyloside as well as two new phenols: an alkylated β-naphthol and a β-methoxy naphthalene in cultured human umbilical vein endothelial cells (HUVEC). Of these, suaedanortriterpenedione (53.7%), normonisesquaterpenol (51.4%) and norditerpenic xyloside (48%) showed the most promising cell proliferative activities compared to others. Moreover, normonisesquaterpenol, norditerpenic xyloside and suaedanortriterpenedione efficiently reversed the oxidative and apoptotic cell damage via downregulation of capase-3/7 by 44.3%, 42.2% and 39.4%, respectively against dichlorofluorescin, whereas by 46.2%, 43.5% and 42.5%, respectively against methylglyoxal. Aminoguanidine, the reference drug inhibited caspase-3/7 activity by 56.2% and 54.7% through attenuation of dichlorofluorescin and methylglyoxal, respectively. Further in silico molecular docking analysis revealed formation of stable complexes between the tested compounds and caspase-3/7. Conclusively, we for the first time demonstrate the growth stimulatory, anti-oxidative and anti-apoptotic salutations of S. monoica derived novel compounds in human endothelial cells. This warrants their further assessment as vascular cell protective and rejuvenating therapeutics, especially in hyperglycemic conditions.

ACS Style

Mohammad K. Parvez; Mohammed S. Al-Dosari; Tabish Rehman; Mohammed F. Alajmi; Ali S. Alqahtani; Mansour S. AlSaid. New terpenic and phenolic compounds from Suaeda monoica reverse oxidative and apoptotic damages in human endothelial cells. Saudi Pharmaceutical Journal 2021, 1 .

AMA Style

Mohammad K. Parvez, Mohammed S. Al-Dosari, Tabish Rehman, Mohammed F. Alajmi, Ali S. Alqahtani, Mansour S. AlSaid. New terpenic and phenolic compounds from Suaeda monoica reverse oxidative and apoptotic damages in human endothelial cells. Saudi Pharmaceutical Journal. 2021; ():1.

Chicago/Turabian Style

Mohammad K. Parvez; Mohammed S. Al-Dosari; Tabish Rehman; Mohammed F. Alajmi; Ali S. Alqahtani; Mansour S. AlSaid. 2021. "New terpenic and phenolic compounds from Suaeda monoica reverse oxidative and apoptotic damages in human endothelial cells." Saudi Pharmaceutical Journal , no. : 1.

Journal article
Published: 29 July 2021 in Molecules
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The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.

ACS Style

Soumaya Touzani; Hamada Imtara; Shankar Katekhaye; Hamza Mechchate; Hayat Ouassou; Ali Alqahtani; Omar Noman; Fahd Nasr; Hugo Fearnley; James Fearnley; Anant Paradkar; Ilham ElArabi; Badiaa Lyoussi. Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities. Molecules 2021, 26, 4589 .

AMA Style

Soumaya Touzani, Hamada Imtara, Shankar Katekhaye, Hamza Mechchate, Hayat Ouassou, Ali Alqahtani, Omar Noman, Fahd Nasr, Hugo Fearnley, James Fearnley, Anant Paradkar, Ilham ElArabi, Badiaa Lyoussi. Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities. Molecules. 2021; 26 (15):4589.

Chicago/Turabian Style

Soumaya Touzani; Hamada Imtara; Shankar Katekhaye; Hamza Mechchate; Hayat Ouassou; Ali Alqahtani; Omar Noman; Fahd Nasr; Hugo Fearnley; James Fearnley; Anant Paradkar; Ilham ElArabi; Badiaa Lyoussi. 2021. "Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities." Molecules 26, no. 15: 4589.

Journal article
Published: 13 July 2021 in Combinatorial Chemistry & High Throughput Screening
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Background: Traditionally, Portulaca oleracea Linn for treating abscesses, dysentery, and also for the treatment of liver diseases. Recent studies have reported its efficacy as neuroprotective, analgesic and anti-inflammatory, a bronchodilator, anticancer, antioxidant, wound healing, besides other important pharmacological actions. Aim and Objective: For curing liver diseases, Chinese use juice from the fresh leaves of Portulaca oleracea Linn, hence to prove this claim an in-vivo hepatoprotective study of extracts from Portulaca oleracea Linn. against carbon tetrachloride hepatotoxic rats. Methods: The in-vitro antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and hepatoprotective activity of extracts of Portulaca oleracea Linn. was assessed against the carbon tetrachloride-induced toxicity rat model. Results: The extracts of Portulaca oleracea Linn produced concentration-dependent percentage inhibition of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical and most significantly by methanol extract. Among the extracts, methanol extract showed a significant decrease in Serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) levels, and a significant increase in total protein (TP) levels against carbon tetrachloride-induced liver toxicity. Conclusion: The results obtained showed that methanol extract of Portulaca oleracea Linn. possesses significant DPPH free radical scavenging and hepatoprotective potential.

ACS Style

Mohd Akbar Dar; Prince Ahad Mir; Mubashir Hussain Masoodi; Ali S. Alqahtani; Nasir A. Siddiqui; Bahar Ahmad. Amelioration of Experimental Hepatotoxicity in Rats by Portulaca oleracea Linn. from Kashmir Himalaya. Combinatorial Chemistry & High Throughput Screening 2021, 24, 1 -1.

AMA Style

Mohd Akbar Dar, Prince Ahad Mir, Mubashir Hussain Masoodi, Ali S. Alqahtani, Nasir A. Siddiqui, Bahar Ahmad. Amelioration of Experimental Hepatotoxicity in Rats by Portulaca oleracea Linn. from Kashmir Himalaya. Combinatorial Chemistry & High Throughput Screening. 2021; 24 ():1-1.

Chicago/Turabian Style

Mohd Akbar Dar; Prince Ahad Mir; Mubashir Hussain Masoodi; Ali S. Alqahtani; Nasir A. Siddiqui; Bahar Ahmad. 2021. "Amelioration of Experimental Hepatotoxicity in Rats by Portulaca oleracea Linn. from Kashmir Himalaya." Combinatorial Chemistry & High Throughput Screening 24, no. : 1-1.

Research article
Published: 10 July 2021 in Journal of Analytical Methods in Chemistry
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The oleo-gum-resin of Commiphora myrrha is one of the most known natural antimicrobial agents, mainly due to its furanosesquiterpenes. A validated method based on sample extraction by matrix solid-phase dispersion (MSPD) followed by high-performance column chromatography (HPLC) determination is applied to analyze two furanosesquiterpenoids, namely, 2-methoxyfuranodiene (CM-1) and 2-acetoxyfuranodiene (CM-2), existing in C. myrrha. The trial parameters that controlled the extraction prospective were studied and optimized. These include the nature of dispersant, mass ratio of sample to the dispersant, and the volume of elution solvent. A comparative antimicrobial study that used the Minimum Inhibitory Concentration Assay (MIC) method between MSPD, ultrasonic, and Soxhlet of myrrh extracts was also conducted. The optimal MSPD parameters used were (i) 15 mL of methanol applied as elution solvent; (ii) silica gel/sample mass at a 2 : 1 ratio; and (iii) a dispersing sorbent selected as silica gel. Technique retrievals were regulated from 96.87% to 100.54%, with relative standard deviations (RSDs) from 1.24% to 4.45%. Commiphora myrrha-MSPD (CM-MSPD) extract showed the highest antibacterial activity against gram-positive and gram-negative bacteria (156.25 μg/mL and 312.5 μg/mL, respectively) and antifungal activity (156.25 μg/mL). Yields acquired through the MSPD technique were larger than yields from other extraction techniques (sonication and traditional reflux extraction methods) with less consumption of time, sample, and solvent. The mode of antibacterial action of CM-1 and CM-2 was elucidated by performing molecular docking with bacterial DNA gyrase. Both the compounds interacted with key residues of DNA gyrase.

ACS Style

Ali S. Alqahtani; Rashed N. Herqash; Omar M. Noman; Tabish Rehman; Abdelaaty A. Shahat; Mohamed F. Alajmi; Fahd A. Nasr. Impact of Different Extraction Methods on Furanosesquiterpenoids Content and Antibacterial Activity of Commiphora myrrha Resin. Journal of Analytical Methods in Chemistry 2021, 2021, 1 -10.

AMA Style

Ali S. Alqahtani, Rashed N. Herqash, Omar M. Noman, Tabish Rehman, Abdelaaty A. Shahat, Mohamed F. Alajmi, Fahd A. Nasr. Impact of Different Extraction Methods on Furanosesquiterpenoids Content and Antibacterial Activity of Commiphora myrrha Resin. Journal of Analytical Methods in Chemistry. 2021; 2021 ():1-10.

Chicago/Turabian Style

Ali S. Alqahtani; Rashed N. Herqash; Omar M. Noman; Tabish Rehman; Abdelaaty A. Shahat; Mohamed F. Alajmi; Fahd A. Nasr. 2021. "Impact of Different Extraction Methods on Furanosesquiterpenoids Content and Antibacterial Activity of Commiphora myrrha Resin." Journal of Analytical Methods in Chemistry 2021, no. : 1-10.

Journal article
Published: 10 June 2021 in Marine Drugs
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The new asperorlactone (1), along with the known illudalane sesquiterpene echinolactone D (2), two known pyrones, 4-(hydroxymethyl)-5-hydroxy-2H-pyran-2-one (3) and its acetate 4, and 4-hydroxybenzaldehyde (5), were isolated from a culture of Aspergillus oryzae, collected from Red Sea marine sediments. The structure of asperorlactone (1) was elucidated by HR-ESIMS, 1D, and 2D NMR, and a comparison between experimental and DFT calculated electronic circular dichroism (ECD) spectra. This is the first report of illudalane sesquiterpenoids from Aspergillus fungi and, more in general, from ascomycetes. Asperorlactone (1) exhibited antiproliferative activity against human lung, liver, and breast carcinoma cell lines, with IC50 values < 100 µM. All the isolated compounds were also evaluated for their toxicity using the zebrafish embryo model.

ACS Style

Raha Orfali; Shagufta Perveen; Muhammad Khan; Atallah Ahmed; Mohammad Wadaan; Areej Al-Taweel; Ali Alqahtani; Fahd Nasr; Sobia Tabassum; Paolo Luciano; Giuseppina Chianese; Jyh-Horng Sheu; Orazio Taglialatela-Scafati. Antiproliferative Illudalane Sesquiterpenes from the Marine Sediment Ascomycete Aspergillus oryzae. Marine Drugs 2021, 19, 333 .

AMA Style

Raha Orfali, Shagufta Perveen, Muhammad Khan, Atallah Ahmed, Mohammad Wadaan, Areej Al-Taweel, Ali Alqahtani, Fahd Nasr, Sobia Tabassum, Paolo Luciano, Giuseppina Chianese, Jyh-Horng Sheu, Orazio Taglialatela-Scafati. Antiproliferative Illudalane Sesquiterpenes from the Marine Sediment Ascomycete Aspergillus oryzae. Marine Drugs. 2021; 19 (6):333.

Chicago/Turabian Style

Raha Orfali; Shagufta Perveen; Muhammad Khan; Atallah Ahmed; Mohammad Wadaan; Areej Al-Taweel; Ali Alqahtani; Fahd Nasr; Sobia Tabassum; Paolo Luciano; Giuseppina Chianese; Jyh-Horng Sheu; Orazio Taglialatela-Scafati. 2021. "Antiproliferative Illudalane Sesquiterpenes from the Marine Sediment Ascomycete Aspergillus oryzae." Marine Drugs 19, no. 6: 333.

Research article
Published: 04 June 2021 in ACS Omega
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Graphene nanocomposites have gained significant interest in a variety of biological applications due to their unique properties. Herein, we have studied the apoptosis-inducing ability and anticancer properties of functionalized highly reduced graphene oxide (HRG) and gold nanoparticles (Au NPs)-based nanocomposites (AP-HRG-Au). Samples were prepared under facile conditions via simple stirring and ultrasonication. All the samples were tested for their anticancer properties against different human cancer cell lines including lung (A549), liver (HepG2), and breast (MCF-7) cancer cells using doxorubicin as a positive control. In order to enhance the solubility and bioavailability of the sample, HRG was functionalized with 1-aminopyrene (1-AP) as a stabilizing ligand. The ligand also facilitated the homogeneous growth of Au NPs on the surface of HRG by offering chemically specific binding sites. The synthesis of nanocomposites and the surface functionalization of HRG were confirmed by UV–Vis, powder X-ray diffraction, and Fourier transform infrared spectroscopy. The structure and morphology of the as-prepared nanocomposites were established by high-resolution transmission electron microscopy. Because of the functionalization, the AP-HRG-Au nanocomposite exhibited enhanced physical stability and high dispersibility. A comparative anticancer study of pristine HRG, nonfunctionalized HRG-Au, and 1-AP-functionalized AP-HRG-Au nanocomposites revealed the enhanced apoptosis ability of functionalized nanocomposites compared to the nonfunctionalized sample, whereas the pristine HRG did not show any anticancer ability against all tested cell lines. Both HRG-Au and AP-HRG-Au have induced a concentration-dependent reduction in cell viability in all tested cell lines after 48 h of exposure, with a significantly higher response in MCF-7 cells compared to the remaining cells. Therefore, MCF-7 cells were selected to perform detailed investigations using apoptosis assay, cell cycle analysis, and reactive oxygen species measurements. These results suggest that AP-HRG-Au induces enhanced apoptosis in human breast cancer cells.

ACS Style

Syed Farooq Adil; Mohammed Rafi Shaik; Fahd A. Nasr; Ali S. Alqahtani; Mohammad Z. Ahmed; Wajhul Qamar; Mufsir Kuniyil; Adibah Almutairi; Abdulrahman Alwarthan; Mohammed Rafiq H. Siddiqui; Mohammad Rafe Hatshan; Mujeeb Khan. Enhanced Apoptosis by Functionalized Highly Reduced Graphene Oxide and Gold Nanocomposites in MCF-7 Breast Cancer Cells. ACS Omega 2021, 6, 15147 -15155.

AMA Style

Syed Farooq Adil, Mohammed Rafi Shaik, Fahd A. Nasr, Ali S. Alqahtani, Mohammad Z. Ahmed, Wajhul Qamar, Mufsir Kuniyil, Adibah Almutairi, Abdulrahman Alwarthan, Mohammed Rafiq H. Siddiqui, Mohammad Rafe Hatshan, Mujeeb Khan. Enhanced Apoptosis by Functionalized Highly Reduced Graphene Oxide and Gold Nanocomposites in MCF-7 Breast Cancer Cells. ACS Omega. 2021; 6 (23):15147-15155.

Chicago/Turabian Style

Syed Farooq Adil; Mohammed Rafi Shaik; Fahd A. Nasr; Ali S. Alqahtani; Mohammad Z. Ahmed; Wajhul Qamar; Mufsir Kuniyil; Adibah Almutairi; Abdulrahman Alwarthan; Mohammed Rafiq H. Siddiqui; Mohammad Rafe Hatshan; Mujeeb Khan. 2021. "Enhanced Apoptosis by Functionalized Highly Reduced Graphene Oxide and Gold Nanocomposites in MCF-7 Breast Cancer Cells." ACS Omega 6, no. 23: 15147-15155.

Research article
Published: 28 May 2021 in Journal of Chemistry
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Phytochemical and pharmacological investigations of Elettaria cardamomum L. were mostly focused on its essential oil and organic crude extracts with little attention on its aqueous extracts. Therefore, the current study aimed to investigate the phytochemical profile, in vitro antiobesity, and antidiabetic activities of the aqueous extract of Elettaria cardamomum L. family Zingiberaceae. UPLC-ESI-MS/MS analysis is used for the aqueous extract characterization in both ionization modes. The analysis revealed the tentative identification of forty-seven compounds based on their MS/MS fragmentation pattern and comparison with the reported data. The identified compounds include eight flavones, thirteen phenolic and nonphenolic acids, one coumarin, and nineteen anthocyanins. Moreover, the in vitro antiobesity and antidiabetic activities were also studied. The results showed that E. cardamomum L. aqueous extract inhibits pancreatic lipase and α-amylase enzyme in a concentration-dependent manner, as 1 mg/mL extract was able to inhibit pancreatic lipase and α-amylase by 62.25% ± 0.58 (IC50 = 288.75 ± 1.3 µg/mL) and 70.42% ± 1.5 (IC50 = 220.5 ± 1.3 µg/mL), respectively. Conclusively, the current study indicated that the investigated biological activities of Elettaria cardamomum aqueous extract were attributed to the existence of biologically active metabolites such as flavones, phenolic and nonphenolic acids, coumarins, and anthocyanins. Moreover, it proposed that the aqueous cardamom extract can be used as a natural potential source in different pharmaceutical preparations to protect against variable chronic disorders, especially obesity and diabetes. Deeper in vivo investigations, isolation, purification, and structural elucidation of the major active metabolites from cardamom are recommended.

ACS Style

Hanan M. Al-Yousef; Ali S. Alqahtani; Wafaa H. B. Hassan; Afraa Alzoubi; Sahar Abdelaziz. Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits. Journal of Chemistry 2021, 2021, 1 -10.

AMA Style

Hanan M. Al-Yousef, Ali S. Alqahtani, Wafaa H. B. Hassan, Afraa Alzoubi, Sahar Abdelaziz. Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits. Journal of Chemistry. 2021; 2021 ():1-10.

Chicago/Turabian Style

Hanan M. Al-Yousef; Ali S. Alqahtani; Wafaa H. B. Hassan; Afraa Alzoubi; Sahar Abdelaziz. 2021. "Chemical Profile, In Vitro Antioxidant, Pancreatic Lipase, and Alpha-Amylase Inhibition Assays of the Aqueous Extract of Elettaria cardamomum L. Fruits." Journal of Chemistry 2021, no. : 1-10.

Journal article
Published: 01 May 2021 in Arabian Journal of Chemistry
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The aim of this study was to optimize the ultrasonication method for efficient extraction of parthenolide from Tarchonanthus camphoratus leaves using Box–Behnken design for response surface methodology (RSM) and then to validate the method by high performance thin layer chromatography (HPTLC). RSM was used to optimize the extraction parameters, i.e., temperature (25–45 °C), time (35–55 min), and liquid-to-solid ratio (16–24 mL/g), to maximize parthenolide yield. Quantitative estimation of parthenolide in ethanol extract (TCEE) of T. camphoratus leaves was conducted to check the effect of all the three variables and validated by HPTLC method using n-hexane and ethyl acetate (3:1, v/v) as mobile phase. A quadratic polynomial model was found to be most suitable with regard to R (parthenolide yield), with R2/%CV = 0.9973/0.4557. The adjusted R2, predicted R2, and signal-to-noise ratio for R were 0.9937, 0.9870, and 47.94, respectively, indicating a high level of tie-in with adequate signal. There was a strong linear correlation between the predicted and experimental R values (R2 = 0.9973). In TCEE, parthenolide was detected with a Rf value of 0.15 at λmax = 590 nm. The optimized ultrasonic extraction produced 1.010% ± 0.04 %w/w with the following extraction parameters: 38.8 °C (M1), 50-min extraction time (M2), and 20.4-mL/g liquid-to-solid ratio (M3). The obtained results of optimization also endorsed by cytotoxicity results (CC50125.47 μg/mL) on human hepatoma cells (HuH7).

ACS Style

Nasir A. Siddiqui; Perwez Alam; Adnan J. Alrehaily; Ali S. Alqahtani; Ali Akhtar; Tawfeq A. Alhowiriny; Omer M. Almarfadi; Ramzi A. Mothana. Optimization of ultrasound-assisted parthenolide extraction from Tarchonanthus camphoratus leaves using response surface methodology: HPTLC and cytotoxicity analysis. Arabian Journal of Chemistry 2021, 14, 103194 .

AMA Style

Nasir A. Siddiqui, Perwez Alam, Adnan J. Alrehaily, Ali S. Alqahtani, Ali Akhtar, Tawfeq A. Alhowiriny, Omer M. Almarfadi, Ramzi A. Mothana. Optimization of ultrasound-assisted parthenolide extraction from Tarchonanthus camphoratus leaves using response surface methodology: HPTLC and cytotoxicity analysis. Arabian Journal of Chemistry. 2021; 14 (6):103194.

Chicago/Turabian Style

Nasir A. Siddiqui; Perwez Alam; Adnan J. Alrehaily; Ali S. Alqahtani; Ali Akhtar; Tawfeq A. Alhowiriny; Omer M. Almarfadi; Ramzi A. Mothana. 2021. "Optimization of ultrasound-assisted parthenolide extraction from Tarchonanthus camphoratus leaves using response surface methodology: HPTLC and cytotoxicity analysis." Arabian Journal of Chemistry 14, no. 6: 103194.

Journal article
Published: 23 March 2021 in Applied Sciences
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Datura innoxia is an important species of Solanaceae family with several purposes in folk medicine. This study intends to explore the cytotoxic effect of D. innoxia on various cancer cell proliferation. D. innoxia ethanolic extract’s effect on the progression of the cell cycle and the induction of apoptosis were investigated by flow cytometry. Further, real-time PCR was employed to confirm apoptosis initiation. In addition, active phytochemicals of D. innoxia was identified by gas chromatography–mass spectroscopy (GC-MS). The cell viability study revealed that the ethanolic extract of D. innoxia demonstrated potent cytotoxicity, with an IC50 value of 10 μg/mL against LoVo colon cancer cells. Cell cycle staining with propidium iodide revealed that D. innoxia treatment leads to cell accumulation in the sub-G1 phase. Using the Annexin V-FITC/PI assay, the ethanolic extract was found to cause a dose-dependent increase in early and late apoptosis when compared to control cells. Apoptosis as the mode of cell death was also confirmed by the increased expression of p53, bax and caspase-8, -9, and -3 along with downregulation of Bcl-2. GC-MS analysis displayed that 3,5-Dihydroxybenzoic acid (16.53%), heneicosyl formate (14.14%), 2,3-dimethyl-3-pentanol (12.89%), 2-hydroxy-4-methyl pentanoic acid (5.19%) were the main phytoconstituents. These findings conclude that D. innoxia causes cell death through apoptosis, suggesting more attention should be paid to further exploration of the active components from D. innoxia responsible for the observed activities.

ACS Style

Mohammed Al-Zharani; Fahd Nasr; Ali Alqahtani; Mary Cordero; Amal Alotaibi; Asmatanzeem Bepari; Saud Alarifi; Ali Daoud; Ibrahim Barnawi; Haytham Daradka. In Vitro Cytotoxic Evaluation and Apoptotic Effects of Datura innoxia Grown in Saudi Arabia and Phytochemical Analysis. Applied Sciences 2021, 11, 2864 .

AMA Style

Mohammed Al-Zharani, Fahd Nasr, Ali Alqahtani, Mary Cordero, Amal Alotaibi, Asmatanzeem Bepari, Saud Alarifi, Ali Daoud, Ibrahim Barnawi, Haytham Daradka. In Vitro Cytotoxic Evaluation and Apoptotic Effects of Datura innoxia Grown in Saudi Arabia and Phytochemical Analysis. Applied Sciences. 2021; 11 (6):2864.

Chicago/Turabian Style

Mohammed Al-Zharani; Fahd Nasr; Ali Alqahtani; Mary Cordero; Amal Alotaibi; Asmatanzeem Bepari; Saud Alarifi; Ali Daoud; Ibrahim Barnawi; Haytham Daradka. 2021. "In Vitro Cytotoxic Evaluation and Apoptotic Effects of Datura innoxia Grown in Saudi Arabia and Phytochemical Analysis." Applied Sciences 11, no. 6: 2864.

Journal article
Published: 29 January 2021 in Applied Sciences
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Resistance to chemotherapy and recurrence are major hurdles to treating hormone receptor-negative breast cancer. The crude extract and natural products obtained from medicinal plants are believed to be multitargeted and possess less toxicity as compared to synthetic compounds. The aerial parts and roots of Moricandia sinaica (Boiss.) Boiss were used to prepare the crude extracts in solvents of different polarities. Human breast cancer cell lines (MCF7 and MDA-MB-231), liver carcinoma (HepG2), and nontumorigenic cells of human origin (human umbilical vein endothelial cells (HUVEC)) were treated with a serial dilution of crude extracts obtained from the aerial and roots of Moricandia sinaica (Boiss.) Boiss. The methanol extract of the shoots exhibited a higher level of cytotoxicity against MDA-MB-231 cells than against any other cancer and nontumorigenic cells lines. Six new compounds were identified by gas chromatography–mass spectrophotometry analysis in the shoots extract of Moricandia sinaica (Boiss.) Boiss, and 2-Tridecen-1-ol was one of the major compounds that represent more than 35% of the extract. M-phase inducer phosphases 1 and 2 (CDC 25A and B) were identified as the specific protein target for 2-Tridecen-1-ol by the Swiss protein target prediction tool. In silico molecular docking showed the binding of 2-Tridecen-1-ol with CDC 25 B with a higher binding energy as compared to CDC 25A. The possible molecular mechanism of anticancer activity of Moricandia sinaica (Boiss.) Boiss in MDA-MB-231 breast cancer is through inhibition of M-phase inducer phosphatases 1 and 2 via 2-Tridecen-1-ol. Further investigations in breast cancer models are needed to explore the therapeutic potential of Moricandia sinaica (Boiss.) Boiss and 2-Tridecen-1-ol as an efficient remedy with a possibly less toxic approach to treat triple-negative breast cancer.

ACS Style

Muhammad Farooq Khan; Fahd Nasr; Almohannad Baabbad; Ali Alqahtani; Mohammad Wadaan. Investigating the Anticancer Activity and Characterization of Bioactive Constituents of Moricandia sinaica (Boiss.) Boiss through In Vitro and In Silico Approaches in Triple-Negative Breast Cancer Cell Line. Applied Sciences 2021, 11, 1244 .

AMA Style

Muhammad Farooq Khan, Fahd Nasr, Almohannad Baabbad, Ali Alqahtani, Mohammad Wadaan. Investigating the Anticancer Activity and Characterization of Bioactive Constituents of Moricandia sinaica (Boiss.) Boiss through In Vitro and In Silico Approaches in Triple-Negative Breast Cancer Cell Line. Applied Sciences. 2021; 11 (3):1244.

Chicago/Turabian Style

Muhammad Farooq Khan; Fahd Nasr; Almohannad Baabbad; Ali Alqahtani; Mohammad Wadaan. 2021. "Investigating the Anticancer Activity and Characterization of Bioactive Constituents of Moricandia sinaica (Boiss.) Boiss through In Vitro and In Silico Approaches in Triple-Negative Breast Cancer Cell Line." Applied Sciences 11, no. 3: 1244.

Journal article
Published: 08 January 2021 in Molecules
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Withania frutescens L. is a wild perennial woody plant used by the local population for diverse therapeutic purposes. This work aims to study for the first time the potential inhibitory effect of this plant hydroethanolic extract on α-amylase and α-glucosidase activities using in vitro methods and its antidiabetic and antihyperglycemic activities using alloxan-induced diabetic mice as a model for experimental diabetes. Two doses were selected for the in vivo study (200 and 400 mg/kg) and glibenclamide, a well-known antidiabetic drug (positive control) in a subacute study (28 days) where the antihyperglycemic activity was also assessed over a period of 12 h on diabetic mice. The continuous treatment of diabetic mice with the extract of Withania frutescens for 4 weeks succeeded to slowly manage their high fasting blood glucose levels (after two weeks), while the antihyperglycemic test result revealed that the extract of this plant did not control hyperglycemia in the short term. No toxicity signs or death were noted for the groups treated with the plant extract, and it shows a protective effect on the liver and kidney. The in vitro assays demonstrated that the inhibition of alpha-amylase and alpha-glucosidase might be one of the mechanisms of action exhibited by the extract of this plant to control and prevent postprandial hyperglycemia. This work indicates that W. frutescens have an important long term antidiabetic effect that can be well established to treat diabetes.

ACS Style

Hamza Mechchate; Imane Es-Safi; Abdelhadi Louba; Ali S. Alqahtani; Fahd A. Nasr; Omar M. Noman; Muhammad Farooq; Mohammed S. Alharbi; Abdulaziz Alqahtani; Amina Bari; Hicham Bekkari; Dalila Bousta. In Vitro Alpha-Amylase and Alpha-Glucosidase Inhibitory Activity and In Vivo Antidiabetic Activity of Withania frutescens L. Foliar Extract. Molecules 2021, 26, 293 .

AMA Style

Hamza Mechchate, Imane Es-Safi, Abdelhadi Louba, Ali S. Alqahtani, Fahd A. Nasr, Omar M. Noman, Muhammad Farooq, Mohammed S. Alharbi, Abdulaziz Alqahtani, Amina Bari, Hicham Bekkari, Dalila Bousta. In Vitro Alpha-Amylase and Alpha-Glucosidase Inhibitory Activity and In Vivo Antidiabetic Activity of Withania frutescens L. Foliar Extract. Molecules. 2021; 26 (2):293.

Chicago/Turabian Style

Hamza Mechchate; Imane Es-Safi; Abdelhadi Louba; Ali S. Alqahtani; Fahd A. Nasr; Omar M. Noman; Muhammad Farooq; Mohammed S. Alharbi; Abdulaziz Alqahtani; Amina Bari; Hicham Bekkari; Dalila Bousta. 2021. "In Vitro Alpha-Amylase and Alpha-Glucosidase Inhibitory Activity and In Vivo Antidiabetic Activity of Withania frutescens L. Foliar Extract." Molecules 26, no. 2: 293.

Journal article
Published: 30 November 2020 in Molecules
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Origanum majorana L. is a plant commonly used in folk medicine to treat depression and several neurological disorders. This study aims to evaluate the antidepressant-like effect of the Origanum majorana L. polyphenols (OMP) obtained from the aerial parts using two different depression model tests: the forced swimming test (FST) and the tail suspension test (TST) in Swiss albino mice. The experiments were performed on days 1, 7, 14, and 21 with daily administration of different treatments. Two different doses were chosen for this study (50 and 100 mg/kg), and paroxetine was used as a positive control. Immobility as a consequence of the depression state was significantly reduced following the treatment with OMP, indicating an antidepressant effect. A subacute toxicity study was also performed following the Organization for Economic Co-operation and Development (OECD) Guidelines (407), showing no sign of toxicity for the studied doses. The phytochemical screening revealed the presence of 12 components, all belonging to polyphenols: arbutin, rosmarinic acid, ursolic acid, quercetin-3-O-glucoside, quercetin-7-O-glucuronic acid, luteolin-7-O-glucoside, kaempferol-3-0-glucuronic acid, Kaempferol-3-0-pentose, caffeic acid, catechin, quercetin, and rutin. These findings suggest that O. majorana has interesting antidepressant-like properties, which deserve further investigation.

ACS Style

Amal Amaghnouje; Hamza Mechchate; Imane Es-Safi; Smahane Boukhira; Ali S. Aliqahtani; Omar M. Noman; Fahd A. Nasr; Raffaele Conte; Anna Calarco; Dalila Bousta. Subacute Assessment of the Toxicity and Antidepressant-Like Effects of Origanum Majorana L. Polyphenols in Swiss Albino Mice. Molecules 2020, 25, 5653 .

AMA Style

Amal Amaghnouje, Hamza Mechchate, Imane Es-Safi, Smahane Boukhira, Ali S. Aliqahtani, Omar M. Noman, Fahd A. Nasr, Raffaele Conte, Anna Calarco, Dalila Bousta. Subacute Assessment of the Toxicity and Antidepressant-Like Effects of Origanum Majorana L. Polyphenols in Swiss Albino Mice. Molecules. 2020; 25 (23):5653.

Chicago/Turabian Style

Amal Amaghnouje; Hamza Mechchate; Imane Es-Safi; Smahane Boukhira; Ali S. Aliqahtani; Omar M. Noman; Fahd A. Nasr; Raffaele Conte; Anna Calarco; Dalila Bousta. 2020. "Subacute Assessment of the Toxicity and Antidepressant-Like Effects of Origanum Majorana L. Polyphenols in Swiss Albino Mice." Molecules 25, no. 23: 5653.

Journal article
Published: 11 November 2020 in Saudi Journal of Biological Sciences
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Current pre-clinical evidences of Centella focus on its pharmacological effects on normal wound healing but there are limited studies on the bioactivity of Centella in cellular dysfunction associated with diabetic wounds. Hence we planned to examine the potential of Centella cordifolia in inhibiting methylglyoxal (MGO)-induced extracellular matrix (ECM) glycation and promoting the related cellular functions. A Cell-ECM adhesion assay examined the ECM glycation induced by MGO. Different cell types that contribute to the healing process (fibroblasts, keratinocytes and endothelial cells) were evaluated for their ability to adhere to the glycated ECM. Methanolic extract of Centella species was prepared and partitioned to yield different solvent fractions which were further analysed by high performance liquid chromatography equipped with photodiode array detector (HPLC-PDA) method. Based on the antioxidant [2,2-diphenyl-1-picrylhydrazyl (DPPH) assay] screening, anti-glycation activity and total phenolic content (TPC) of the different Centella species and fractions, the ethyl acetate fraction of C. cordifolia was selected for further investigating its ability to inhibit MGO-induced ECM glycation and promote cellular distribution and adhesion. Out of the three Centella species (C. asiatica, C. cordifolia and C. erecta), the methanolic extract of C. cordifolia showed maximum inhibition of Advanced glycation end products (AGE) fluorescence (20.20 ± 4.69 %, 25.00 ± 3.58 % and 16.18 ± 1.40 %, respectively). Its ethyl acetate fraction was enriched with phenolic compounds (3.91 ± 0.12 mg CAE/μg fraction) and showed strong antioxidant (59.95 ± 7.18 μM TE/μg fraction) and antiglycation activities. Improvement of cells spreading and adhesion of endothelial cells, fibroblasts and keratinocytes was observed for ethyl acetate treated MGO-glycated extracellular matrix. Significant reduction in attachment capacity of EA.hy926 cells seeded on MGO-glycated fibronectin (41.2%) and attachment reduction of NIH3t3 and HaCaT cells seeded on MGO-glycated collagen (33.7% and 24.1%, respectively) were observed. Our findings demonstrate that ethyl acetate fraction of C. cordifolia was effective in attenuating MGO-induced glycation and cellular dysfunction in the in-vitro wound healing models suggesting that C. cordifolia could be a potential candidate for diabetic wound healing. It could be subjected for further isolation of new phytoconstituents having potential diabetic wound healing properties.

ACS Style

Ali S. Alqahtani; Kong M. Li; Valentina Razmovski-Naumovski; Antony Kam; Perwez Alam; George Q. Li. Attenuation of methylglyoxal-induced glycation and cellular dysfunction in wound healing by Centella cordifolia. Saudi Journal of Biological Sciences 2020, 28, 813 -824.

AMA Style

Ali S. Alqahtani, Kong M. Li, Valentina Razmovski-Naumovski, Antony Kam, Perwez Alam, George Q. Li. Attenuation of methylglyoxal-induced glycation and cellular dysfunction in wound healing by Centella cordifolia. Saudi Journal of Biological Sciences. 2020; 28 (1):813-824.

Chicago/Turabian Style

Ali S. Alqahtani; Kong M. Li; Valentina Razmovski-Naumovski; Antony Kam; Perwez Alam; George Q. Li. 2020. "Attenuation of methylglyoxal-induced glycation and cellular dysfunction in wound healing by Centella cordifolia." Saudi Journal of Biological Sciences 28, no. 1: 813-824.

Journal article
Published: 11 November 2020 in Saudi Journal of Biological Sciences
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To investigate the effect of the ethyl acetate fraction of the aerial parts of E. paralias L. F. Euphorbiaceae on nephroprotective, cytotoxic, and antioxidant. different spectroscopic and spectrophotometric methods were applied to identify phytoconstituents. The nephroprotective potential of E. paralias ethyl acetate fraction (Ep EtOAc) was evaluated in male rats with thioacetamide-induced kidney injury, as wll as cytotoxic activity was evaluated using a viability assay, and the antioxidant activity was evaluated using the DPPH method. Results: quantitative estimation of total phenolics and flavonoids of E. paralias was performed using unique spectrophotometric methods. The polyphenolic compounds gallic acid (1), ellagic acid (2), kaempferol-3-O-(6″-O-galloyl-β-D-glucopyranoside) (3), quercetin-3-O-β-D-glucopyranoside (4) and quercetin-3-O-β-D-arabinoside (5) were isolated from the ethyl acetate fraction of the aerial parts of E. paralias. The thioacetamide administration resulted in marked nephrotoxicity, but pretreatment with Ep EtOAc significantly attenuated the nephrotoxicity through alteration of kidney biomarkers, thereby improving the redox status of the tissue and restoring serum biochemical parameters nearly to normal levels. This study revealed a significant cytotoxic and strong antioxidant effect. Conclusion: we conclude that the Ep EtOAc may be used in the future as nephroprotective, cytotoxic, and antioxidant agent derived from a natural source.

ACS Style

Hanan M. Al-Yousef; Ali S. Alqahtani; Afaf S.A. Ghani; Sayed A. El-Toumy; Wagdi I.A. El-Dougdoug; Wafaa H.B. Hassan; Hanaa M. Hassan. Nephroprotective, cytotoxic and antioxidant activities of Euphorbia paralias. Saudi Journal of Biological Sciences 2020, 28, 785 -792.

AMA Style

Hanan M. Al-Yousef, Ali S. Alqahtani, Afaf S.A. Ghani, Sayed A. El-Toumy, Wagdi I.A. El-Dougdoug, Wafaa H.B. Hassan, Hanaa M. Hassan. Nephroprotective, cytotoxic and antioxidant activities of Euphorbia paralias. Saudi Journal of Biological Sciences. 2020; 28 (1):785-792.

Chicago/Turabian Style

Hanan M. Al-Yousef; Ali S. Alqahtani; Afaf S.A. Ghani; Sayed A. El-Toumy; Wagdi I.A. El-Dougdoug; Wafaa H.B. Hassan; Hanaa M. Hassan. 2020. "Nephroprotective, cytotoxic and antioxidant activities of Euphorbia paralias." Saudi Journal of Biological Sciences 28, no. 1: 785-792.

Journal article
Published: 30 October 2020 in Molecules
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In this study, the aerial parts of Moricandia sinaica were evaluated for their in vivo analgesic, anti-inflammatory and antipyretic activities. The analgesic activities were examined using acetic acid-induced writhing, the hot plate test and the tail flick method. The anti-inflammatory and the antipyretic activities were evaluated using carrageenan-induced paw edema in rats and brewer’s yeast-induced pyrexia in mice, respectively. The aqueous fraction of the methanol extract (MS-3) showed to be the most bioactive among the other investigated fractions. At the dose of 500 mg/kg, the fraction (MS-3) showed a significant percentage inhibition of the carrageenan-induced edema by 52.4% (p < 0.05). In addition, MS-3 exhibited a significant inhibition of acetic acid-induced writhes by 44.4% and 61.5% (p < 0.001) at 250-mg/kg and 500-mg/kg doses, respectively. At 120 min post-treatment, the rat groups treated with MS-3 displayed statistically significant reduction in rectal temperature (p < 0.001) by 1.7 °C and 2.2 °C at 250- and 500-mg/kg doses, respectively. The phytochemical composition of the fraction (MS-3) was characterized by high-performance liquid chromatography-mass spectrometry (HPLC-PDA-MS/MS). Molecular docking studies demonstrated that the polyphenols identified in MS-3 revealed good binding energy upon docking to some target proteins involved in pain response and inflammation, such as the cannabinoid receptors CB1 and CB2, the fatty acid amide hydrolase (FAAH) and the cyclooxygenase COX-1 and COX-2 enzymes. Based on the findings from the present work, it could be concluded that the aerial parts extract of M. sinaica exerts potential analgesic, anti-inflammatory and antipyretic effects in rats.

ACS Style

Sahar El-Mekkawy; Abdelaaty A. Shahat; Ali S. Alqahtani; Mansour S. Alsaid; Mohamed A.O. Abdelfattah; Riaz Ullah; Mahmoud Emam; Abdelaziz Yasri; Mansour Sobeh. A Polyphenols-Rich Extract from Moricandia sinaica Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo. Molecules 2020, 25, 5049 .

AMA Style

Sahar El-Mekkawy, Abdelaaty A. Shahat, Ali S. Alqahtani, Mansour S. Alsaid, Mohamed A.O. Abdelfattah, Riaz Ullah, Mahmoud Emam, Abdelaziz Yasri, Mansour Sobeh. A Polyphenols-Rich Extract from Moricandia sinaica Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo. Molecules. 2020; 25 (21):5049.

Chicago/Turabian Style

Sahar El-Mekkawy; Abdelaaty A. Shahat; Ali S. Alqahtani; Mansour S. Alsaid; Mohamed A.O. Abdelfattah; Riaz Ullah; Mahmoud Emam; Abdelaziz Yasri; Mansour Sobeh. 2020. "A Polyphenols-Rich Extract from Moricandia sinaica Boiss. Exhibits Analgesic, Anti-Inflammatory and Antipyretic Activities In Vivo." Molecules 25, no. 21: 5049.

Journal article
Published: 29 October 2020 in Molecules
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Ziziphus nummularia is an important source of valuable phytoconstituents, which are widely used in traditional medicine system of Indo-Pak sub-continent. In this study we investigated the distribution of phenolic compounds in the fruit pericarps of six different genotypes (ZNP01-06) of Z. nummularia growing in the unexplored hilly areas of Pakistan. The methanolic extracts of these genotypes were screened for total phenolic content (TPC), total flavonoid content (TFC), antioxidant, and cholinesterase inhibitory potentials. The observed biological potentials were explained in terms of the outcome of molecular docking and HPLC analyses. Among them, genotype ZNP02 displayed high TPC (88.50 ± 1.23 μg/mL) and showed potent scavenging activity against DPPH (67.03 ± 1.04 μg/mL) and ABTS (65.3 ± 1.74 μg/mL) in comparison to ascorbic acid (68.7 ± 0.47 μg/mL). Moreover, genotypes ZNP01, ZNP02, and ZNP04 displayed potent inhibition against acetyl and butyryl cholinesterases (AChE and BChE) with IC50 values of 21.2, 20.5, and 23.7 μg/mL (AChE) and 22.7, 24.4, and 33.1 μg/mL (BChE), respectively. Furthermore, the individual compounds in the most potent species ZNP01 responsible for potent enzyme inhibition (identified through HPLC-UV analysis), were computed via docking simulation software to the enzyme structures. Among these compounds rutin exhibited significant binding affinity with value of −9.20 kcal/mol. The differences amongst the phytochemical compositions of the selected genotypes highlighted the genotypic variations in them. Based on our results it was concluded that the selected plant can be used as remedy of oxidative stress and neurodegenerative diseases. However, further studies are needed to isolate responsible compounds and test the observed potential in vivo, along with toxicological evaluations in animal models.

ACS Style

Nisar Uddin; Niaz Ali; Zia Uddin; Nausheen Nazir; Muhammad Zahoor; Umer Rashid; Riaz Ullah; Ali S. Alqahtani; Abdulaziz M. Alqahtani; Fahd A. Nasr; Mengjun Liu; Mohammad Nisar. Evaluation of Cholinesterase Inhibitory Potential of Different Genotypes of Ziziphus nummularia, Their HPLC-UV, and Molecular Docking Analysis. Molecules 2020, 25, 5011 .

AMA Style

Nisar Uddin, Niaz Ali, Zia Uddin, Nausheen Nazir, Muhammad Zahoor, Umer Rashid, Riaz Ullah, Ali S. Alqahtani, Abdulaziz M. Alqahtani, Fahd A. Nasr, Mengjun Liu, Mohammad Nisar. Evaluation of Cholinesterase Inhibitory Potential of Different Genotypes of Ziziphus nummularia, Their HPLC-UV, and Molecular Docking Analysis. Molecules. 2020; 25 (21):5011.

Chicago/Turabian Style

Nisar Uddin; Niaz Ali; Zia Uddin; Nausheen Nazir; Muhammad Zahoor; Umer Rashid; Riaz Ullah; Ali S. Alqahtani; Abdulaziz M. Alqahtani; Fahd A. Nasr; Mengjun Liu; Mohammad Nisar. 2020. "Evaluation of Cholinesterase Inhibitory Potential of Different Genotypes of Ziziphus nummularia, Their HPLC-UV, and Molecular Docking Analysis." Molecules 25, no. 21: 5011.

Journal article
Published: 13 October 2020 in Brain Sciences
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The production and up-regulation of inflammatory mediators are contributing factors for the development and maintenance of neuropathic pain. In the present study, the post-treatment of synthetic 1,3,4 oxadiazole derivative (B3) for its neuroprotective potential in chronic constriction injury-induced neuropathic pain was applied. In-silico studies were carried out through Auto Dock, PyRx, and DSV to obtain the possible binding and interactions of the ligands (B3) with COX-2, IL-6, and iNOS. The sciatic nerve of the anesthetized rat was constricted with sutures 3/0. Treatment with 1,3,4-oxadiazole derivative was started a day after surgery and continued until the 14th day. All behavioral studies were executed on day 0, 3rd, 7th, 10th, and 14th. The sciatic nerve and spinal cord were collected for further molecular analysis. The interactions in the form of hydrogen bonding stabilizes the ligand target complex. B3 showed three hydrogen bonds with IL-6. B3, in addition to correcting paw posture/deformation induced by CCI, attenuates hyperalgesia (p < 0.001) and allodynia (p < 0.001). B3 significantly raised the level of GST and GSH in both the sciatic nerve and spinal cord and reduced the LPO and iNOS (p < 0.001). B3 attenuates the pathological changes induced by nerve injury, which was confirmed by H&E staining and IHC examination. B3 down-regulates the over-expression of the inflammatory mediator IL-6 and hence provides neuroprotective effects in CCI-induced pain. The results demonstrate that B3 possess anti-nociceptive and anti-hyperalgesic effects and thus minimizes pain perception and inflammation. The possible underlying mechanism for the neuroprotective effect of B3 probably may be mediated through IL-6.

ACS Style

Muhammad Faheem; Syed Ali; Abdul Khan; Mahboob Alam; Umair Ilyas; Muhammad Zahoor; Muhammad Sahibzada; Sidra Khalid; Riaz Ullah; Ali Alqahtani; Abdulaziz Alqahtani. 1,3,4-Oxadiazole Derivative Attenuates Chronic Constriction Injury Induced Neuropathic Pain: A Computational, Behavioral, and Molecular Approach. Brain Sciences 2020, 10, 731 .

AMA Style

Muhammad Faheem, Syed Ali, Abdul Khan, Mahboob Alam, Umair Ilyas, Muhammad Zahoor, Muhammad Sahibzada, Sidra Khalid, Riaz Ullah, Ali Alqahtani, Abdulaziz Alqahtani. 1,3,4-Oxadiazole Derivative Attenuates Chronic Constriction Injury Induced Neuropathic Pain: A Computational, Behavioral, and Molecular Approach. Brain Sciences. 2020; 10 (10):731.

Chicago/Turabian Style

Muhammad Faheem; Syed Ali; Abdul Khan; Mahboob Alam; Umair Ilyas; Muhammad Zahoor; Muhammad Sahibzada; Sidra Khalid; Riaz Ullah; Ali Alqahtani; Abdulaziz Alqahtani. 2020. "1,3,4-Oxadiazole Derivative Attenuates Chronic Constriction Injury Induced Neuropathic Pain: A Computational, Behavioral, and Molecular Approach." Brain Sciences 10, no. 10: 731.

Journal article
Published: 12 September 2020 in Saudi Pharmaceutical Journal
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Increasing incidence of multi-drug resistant bacterial pathogens, especially in clinical settings, has been developed into a grave health situation. The drug resistance problem demands the necessity for alternative unique therapeutic policies. One such tactic is targeting the quorum sensing (QS) controlled virulence and biofilm production. In this study, we evaluated a marine steroid Siphonocholin (Syph-1) isolated from Siphonochalina siphonella against Chromobacterium violaceum (CV) 12472, Pseudomonas aeruginosa (PAO1), Methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii (BAA) for biofilm and pellicle formation inhibition, and anti-QS property. MIC of Syph-1 against MRSA, CV, PAO1 was found as 64 µg/mL and 256 µg/mL against BAA. At selected sub-MICs, Syph-1 significantly (P ≤ 0.05) decreased the production of QS regulated virulence functions of CV12472 (violacein) and PAO1 [elastase, total protease, pyocyanin, chitinase, exopolysaccharides, and swarming motility]. The Syph-1 significantly decreased (p = 0.005) biofilm formation ability of tested bacterial pathogens, at sub-MIC level (PAO1 > MRSA > CV > BAA) and pellicle formation in A. baumannii (at 128 µg/mL). Molecular docking and simulation results indicated that Siph-1 was bound at the active site of BfmR N-terminal domain with high affinity. This study highlights the anti-QS and anti-biofilm activity of Syph-1 against bacterial pathogens reflecting its broad spectrum anti-infective potential.

ACS Style

Perwez Alam; Ali S. Alqahtani; Fohad Mabood Husain; Tabish Rehman; Mohamed F. Alajmi; Omar M. Noman; Ali A. El Gamal; Shaza M. Al-Massarani; Mohammad Shavez Khan. Siphonocholin isolated from red sea sponge Siphonochalina siphonella attenuates quorum sensing controlled virulence and biofilm formation. Saudi Pharmaceutical Journal 2020, 28, 1383 -1391.

AMA Style

Perwez Alam, Ali S. Alqahtani, Fohad Mabood Husain, Tabish Rehman, Mohamed F. Alajmi, Omar M. Noman, Ali A. El Gamal, Shaza M. Al-Massarani, Mohammad Shavez Khan. Siphonocholin isolated from red sea sponge Siphonochalina siphonella attenuates quorum sensing controlled virulence and biofilm formation. Saudi Pharmaceutical Journal. 2020; 28 (11):1383-1391.

Chicago/Turabian Style

Perwez Alam; Ali S. Alqahtani; Fohad Mabood Husain; Tabish Rehman; Mohamed F. Alajmi; Omar M. Noman; Ali A. El Gamal; Shaza M. Al-Massarani; Mohammad Shavez Khan. 2020. "Siphonocholin isolated from red sea sponge Siphonochalina siphonella attenuates quorum sensing controlled virulence and biofilm formation." Saudi Pharmaceutical Journal 28, no. 11: 1383-1391.

Journal article
Published: 03 August 2020 in Separations
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The cytotoxic effects of the crude extract of Loranthus acaciae Zucc. and its n-hexane, chloroform, and n-butanol fractions were assessed against three cancer cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Cell apoptosis was determined using an annexin V-phycoerythrin/7-aminoactinomycin kit. We observed that the L. acaciae n-hexane extract (LAHE) could inhibit cancer cell growth, particularly of MCF7 and A549 cells. Chromatographic purification of LAHE and nuclear magnetic resonance analysis led to the identification of two compounds from this plant species, namely, betulinic acid and β-sitosterol, for the first time. Flow cytometry study suggested that betulinic acid induced cell death via apoptosis, as a distinguished marked enhancement in the early and late apoptosis of human lung (A549) and breast (MCF-7) cancer cell lines. The isolated compounds were further estimated concurrently in LAHE using a validated high-performance thin-layer chromatographic (HPTLC) method on a 10 × 10 cm2 HPTLC plate with chloroform, methanol, and glacial acetic acid (97:2:1, v/v/v) as the mobile phase and a λmax of 540 nm. The amounts of betulinic acid and β-sitosterol in LAHE were 69.46 and 135.53 µg/mg of dried weight of extract, respectively. The excellent cytotoxic effect of LAHE could be attributed to the presence of ample amounts of betulinic acid.

ACS Style

Omar M. Noman; Fahd A. Nasr; Ramzi A. Mothana; Ali S. Alqahtani; Wajhul Qamar; Abdullah A. Al-Mishari; Adnan J. Al-Rehaily; Nasir A. Siddiqui; Perwez Alam; Omer M. Almarfadi. Isolation, Characterization, and HPTLC-Quantification of Compounds with Anticancer Potential from Loranthus Acaciae Zucc. Separations 2020, 7, 43 .

AMA Style

Omar M. Noman, Fahd A. Nasr, Ramzi A. Mothana, Ali S. Alqahtani, Wajhul Qamar, Abdullah A. Al-Mishari, Adnan J. Al-Rehaily, Nasir A. Siddiqui, Perwez Alam, Omer M. Almarfadi. Isolation, Characterization, and HPTLC-Quantification of Compounds with Anticancer Potential from Loranthus Acaciae Zucc. Separations. 2020; 7 (3):43.

Chicago/Turabian Style

Omar M. Noman; Fahd A. Nasr; Ramzi A. Mothana; Ali S. Alqahtani; Wajhul Qamar; Abdullah A. Al-Mishari; Adnan J. Al-Rehaily; Nasir A. Siddiqui; Perwez Alam; Omer M. Almarfadi. 2020. "Isolation, Characterization, and HPTLC-Quantification of Compounds with Anticancer Potential from Loranthus Acaciae Zucc." Separations 7, no. 3: 43.

Original research
Published: 01 May 2020 in International Journal of Nanomedicine
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Aim: With the rapid emergence of antibiotic resistance, efforts are being made to obtain new selective antimicrobial agents. Hybridization between quinazolinone and benzenesulfonamide can provide new antimicrobial candidates. Also, the use of nanoparticles can help boost drug efficacy and lower side effects. Materials and Methods: Novel quinazolinone-benzenesulfonamide derivatives 5– 18 were synthesized and screened for their antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, MRSA and yeast. The most potent compound 16 was conjugated with copper oxide nanoparticles 16-CuONPs by gamma irradiation (4.5 KGy). Characterization was performed using UV–Visible, TEM examination, XRD patterns and DLS. Moreover, compound 16 was used to synthesize two nanoformulations: 16-CNPs by loading 16 in chitosan nanoparticles and the nanocomposites 16-CuONPs-CNPs. Characterization of these nanoformulations was performed using TEM and zeta potential. Besides, the inhibitory profile against Staphylococcus aureus DNA gyrase was assayed. Cytotoxic evaluation of 16, 16-CNPs and 16-CuONPs-CNPs on normal VERO cell line was carried out to determine its relative safety. Molecular docking of 16 was performed inside the active site of S. aureus DNA gyrase. Results: Compound 16 was the most active in this series against all the tested strains and showed inhibition zones and MICs in the ranges of 25– 36 mm and 0.31– 5.0 μg/mL, respectively. The antimicrobial screening of the synthesized nanoformulations revealed that 16-CuONPs-CNPs displayed the most potent activity. The MBCs of 16 and the nanoformulations were measured and proved their bactericidal mode of action. The inhibitory profile against S. aureus DNA gyrase showed IC50 ranging from 10.57 to 27.32 μM. Cytotoxic evaluation of 16, 16-CNPs and 16-CuONPs-CNPs against normal VERO cell lines proved its relative safety (IC50= 927, 543 and 637 μg/mL, respectively). Molecular docking of 16 inside the active site of S. aureus DNA gyrase showed that it binds in the same manner as that of the co-crystallized ligand, ciprofloxacin. Conclusion: Compound 16 could be considered as a new antimicrobial lead candidate with enhanced activity upon nanoformulation.

ACS Style

Mostafa M Ghorab; Ali S Alqahtani; Aiten M Soliman; Ahmed A Askar. Novel N-(Substituted) Thioacetamide Quinazolinone Benzenesulfonamides as Antimicrobial Agents. International Journal of Nanomedicine 2020, ume 15, 3161 -3180.

AMA Style

Mostafa M Ghorab, Ali S Alqahtani, Aiten M Soliman, Ahmed A Askar. Novel N-(Substituted) Thioacetamide Quinazolinone Benzenesulfonamides as Antimicrobial Agents. International Journal of Nanomedicine. 2020; ume 15 ():3161-3180.

Chicago/Turabian Style

Mostafa M Ghorab; Ali S Alqahtani; Aiten M Soliman; Ahmed A Askar. 2020. "Novel N-(Substituted) Thioacetamide Quinazolinone Benzenesulfonamides as Antimicrobial Agents." International Journal of Nanomedicine ume 15, no. : 3161-3180.