This work presents refined, updated subregional and regional non-indigenous species (NIS) inventories for the Mediterranean Sea, validated by national and taxonomic experts, with species records observed until December 2020. These datasets will be used as the baselines for the implementation of the Integrated Monitoring and Assessment Programme for the Mediterranean (IMAP) and the Mediterranean Quality Status Report 2023. In total, 1006 non-indigenous species have been found in Mediterranean marine and brackish waters. The highest numbers of NIS were observed in Israel, Türkiye, Lebanon and Italy. Approximately 45 species were categorized as data deficient, either due to lack of consensus on their alien status or the validity of their identification. Polychaeta, Foraminifera and macroalgae were the groups with the highest numbers of controversial species. There was a general increase in the yearly rate of new NIS introductions after the late 1990s, which appears to be slowing down in the last decade, but this may be confounded by reporting lags and differential research efforts. Between 1970 and 2020 there has been a steep increase in the proportion of shared species present throughout all four Mediterranean subregions, which are predominantly transported via shipping and recreational boating. While Lessepsian species are gradually spreading westwards and northwards, there is still a considerable invasion debt accumulating in the eastern and central Mediterranean.

Invasive alien species are a major worldwide driver of biodiversity change. The current study lists verified records of non-indigenous species (NIS) in European marine waters until 2020, with the purpose of establishing a baseline, assessing trends, and discussing appropriate threshold values for good environmental status (GES) according to the relevant European legislation. All NIS records were verified by national experts and trends are presented in six-year assessment periods from 1970 to 2020 according to the European Union Marine Strategy Framework Directive. Altogether, 874 NIS have been introduced to European marine waters until 2020 with the Mediterranean Sea and North-East Atlantic Ocean hosting most of the introductions. Overall, the number of new introductions has steadily increased since 2000. The annual rate of new introductions reached 21 new NIS in European seas within the last six-year assessment period (2012–2017). This increase is likely due to increased human activities and research efforts that have intensified during the early 21st century within European Seas. As Europe seas are not environmentally, nor geographically homogenous, the setting of threshold values for assessing GES requires regional expertise. Further, once management measures are operational, pathway-specific threshold values would enable assessing the effectiveness of such measures.

The patterns of occurrence and bathymetric distribution of epibenthic crustaceans on the continental shelf and upper slope down to a depth of 800 m are analysed based on data gathered during six demersal trawl surveys performed annually in spring along the Mediterranean coasts of the Iberian Peninsula (from the Straits of Gibraltar to Cape Creus) between 1994 and 1999. A total of 598 valid hauls has been studied providing a total of 108 species of decapods, two stomatopods, one euphausiid, one mysid and one isopod. The study area has been subdivided into seven sectors according to their geomorphological characteristics, and the patterns of occurrence and abundance by depth have been analysed separately for each of the sectors. Detailed data on bathymetric distribution are presented for each species. Two main biogeographical areas can be discerned along the study area, which can approximately be separated at Palos Cape: the Alborán Sea to the southwest, and the northwestern Mediterranean (Levantine and Catalan Seas) northwest of Palos Cape. The continental shelf in the Alborán Sea (the most western area of the Mediterranean) is extremely narrow whereas it is much wider in the northwestern Mediterranean. The influence of Atlantic waters entering the Mediterranean is particularly strong in the Alborán Sea which shows a particularly high species richness of Atlantic affinity. Within the context of the western Mediterranean Sea, the Alborán Sea region shows important faunistic characteristics such that it might be considered as a possible separate natural management area for demersal fisheries.

To reduce the practice of discarding commercially fished organisms, several measures such as a discard ban and extra allowances on top of landings quotas (“catch quota”) have been proposed by the European Commission. However, for their development and successful implementation, an understanding of discard patterns on a European scale is needed. In this study, we present an inter-national synthesis of discard data collected on board commercial, towed-gear equipped vessels operating under six different national flags spanning from the Baltic to the Mediterranean Seas mainly between 2003 and 2008. We considered discarded species of commercial value such as Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), European hake (Merluccius merluccius), and European plaice (Pleuronectes platessa). Comparisons of discard per unit effort rates expressed as numbers per hour of fishing revealed that in the Mediterranean Sea minimum size-regulated species such as hake are generally discarded in much lower numbers than elsewhere. For most species examined, variability in discard rates across regions was greater than across fisheries, suggesting that a region-by-region approach to discard reduction would be more relevant. The high uncertainty in discard rate estimates suggests that current sampling regimes should be either expanded or complemented by other data sources, if they are to be used for setting catch quotas.