This article aims to prove dentin bridge formation in two cases after direct pulp capping in reversible pulpitis using the platelet concentrate A-PRF+ and preservation of the vitality of the dental pulp. The hemostasis process for the pulp wound and cavity disinfection with gaseous ozone was performed under anesthesia. A large A-PRF+ membrane was prepared from blood plasma and applied to the pulp wound. After placing an MTA, the cavity was closed using glass-ionomer cement. Clinical and cone beam computed tomography findings demonstrated the formation of a dentin bridge in both cases. After the definitive restoration was conducted during the sixth month, the teeth from both patients were asymptomatic and had normal electric pulp testing values. Conclusions: Via clinical and CBCT examinations, we observed the dentin bridge formation after placing the platelet concentrate A-PRF+ in both cases. The vitality of the dental pulp was preserved. Further research is needed to refine the clinical protocol, recommended period for control examination, clarification of the precise indications of platelet concentrates, etc.

The purpose of this clinical study was to investigate the types of microorganisms in deep proximal caries lesions and the efficacy of elimination of microorganisms after a 24 s ozone application to proximal cavity lesions prepared for restoration. Sixteen caries-active volunteers (female: 8, male: 8; age range: 35–55), with proximally situated deep caries lesions on premolars or molars, were included in the study. Each patient underwent two microbiological investigations. First, the caries decay before treatment was assessed. Second, the dentine tissue was assessed after the removal of necrotic tooth decay and 24 s ozone application. The prevalence of oral streptococci was determined. In addition, we isolated Candida albicans, Enterococcus faecalis and Peptostreptococcus spp. from deep proximal caries lesion in caries-active patients. All microorganisms found in the deep proximal caries lesions were destroyed following 24 s ozone application. Our findings suggest that S. anginosus group, C. albicans and E. faecalis should be considered as an index for caries activity in caries-active patients, but additional studies are necessary to confirm this suggestion. This study demonstrated that 24 s of gaseous ozone application to the deep proximal caries lesions effectively eliminated microbial species.

Background: The ability to self-assess is a critical skill that all health professionals must be able to do, in order to achieve competence. This is essential for the doctors of dental medicine. During their education and practice they apply different clinical and para-clinical procedures. \ud The aim is to evaluate the students' self-assessment skills during the education of clinic and pre-clinic of prosthetic dentistry.\ud Material and methods: After the completion of certain work - preparation for full veneer crown, a questionnaire was provided to each student in preclinical course (n=30) and clinical course (n=30) for self-evaluation. The questionnaire involved: axial reduction, occlusal reduction, facial and lingual reduction, smoothing and finishing. The answers were based on the standard for the university grading scale. Than, the same questionnaire was fulfilled by the assistant professor without seeing students' self-evaluation.\ud Results and Discussion: Results have been reported in percentages. 100% respond rate has been achieved. The students from the preclinical course tend to overestimate their performance (50%). The students from the clinical course tend to submit overall lower grades than the faculty evaluation (25%).\ud Conclusions: The students from clinics have better self-assessment skills. The discrepancy was most pronounced in the junior students. The different evaluations (self-assessment and assistant professor's) help students to improve their understanding of certain principles and improve the teaching effectiveness of education of prosthetic dentistry

The development of regenerative endodontic therapy offers new opportunities for faster tooth recovery in chronic apical periodontitis. This study aimed to use three different surgical protocols to compare the results (filling the apical lesion with bone tissue over 6 months) of treatment in patients with chronic apical periodontitis. This study included 23 patients and 30 teeth with chronic apical periodontitis. The patients were divided into three groups: (1) Endodontic treatment and apical osteotomy without filling; (2) Endodontic treatment and apical osteotomy with the placement of blood concentrate according to the advanced platelet-rich fibrin A-PRF + protocol; (3) Endodontic treatment and apical osteotomy with the placement of blood concentrate according to the ‘sticky bone’ and A-PRF + protocols with allograft. The results were reported after 6 months using cone-beam computed tomography. The results showed a statistically significant difference (Mann–Whitney U test) in the filling of the bone defect in the healing process between the first group (average rank 31.490) and the second group (average rank 96.015), as well as between the first group (average rank 31.490) and the third group (average rank 91.765). No significant difference was found in the filling of the bone defects between the second and third groups. We show that placement of A-PRF + blood concentrate alone or combined with alloplastic graft after classical apical osteotomy of teeth with chronic apical periodontitis leads to a size reduction of the bone defect with 95.25% for group 2 and 90.80% for group 3 after 6 months.

Contemporary indirect restorative materials vary in their physical and mechanical properties, necessitating additional research. This investigation aims to compare the mechanical properties (such as fatigue and compressive strength) of indirect dental restorative materials. In an in vitro study, the mechanical behaviour of monolithic onlay restorations made from a lithium disilicate glass–ceramic (Group A), a ceramic-infiltrated composite (Group B), a polymer-based composite resin (Group C), and zirconia (Group D), bonded to a prepared tooth model, was evaluated after ageing and mechanical cycling. The average value of compressive strength (stage of cracking) in each group was as follows: Group A, 871 N; Group B, 728 N; Group C, 2655 N; and Group D, 2005 N. Moreover, the results of the compressive strength test (stage of destruction) in each group were as follows: Group A, 2516.5 N; Group B, 2266 N; Group C, 5670 N; and Group D, 3543 N. An analysis of variance (ANOVA) followed by Tukey’s (HSD) post hoc test was conducted to assess pairwise comparisons among group means. Statistical analysis revealed significant differences between Groups C and D and the others, highlighting the potential of these materials in clinical applications. Based on the average values, it can be concluded that the 3D-printed ceramic-infiltrated composite onlays exhibited the highest compressive resistance values among the materials evaluated. The lithium disilicate glass–ceramic and the ceramic-infiltrated composite are brittle materials, which should be considered when covering teeth with high occlusal stress.

This study aimed to evaluate the micro-tensile bond strength (µTBS) of two types of composites for indirect restoration, luted to enamel and dentin with self-adhesive cement. Moreover, it aimed to evaluate the impact of thermocycling on bond strength. Sixteen flat enamel and dentin surfaces of human molars were cemented to equal flat specimens of the laboratory composite Signum ceramis and the CAD/CAM block Cerasmart. Half of the specimens of the group underwent thermocycling. After that, the samples were cut into 80 beams for µTBS analysis. The data were analyzed using Levene’s test and the independent sample t-test. The micro-tensile bond strength tests revealed that thermocycling significantly reduced the adhesive bond. Dentin bonds better to conventional laboratory composites. Enamel bonds are better than composite blocks for milling.