The study aimed to evaluate the potential protection against fractures of oral Q10 supplementation in the tibias of rats exposed to nicotine. Nicotine is known to negatively impact bone density and increase the risk of fractures, in addition to affecting other systems such as the gastrointestinal system, impairing its absorption capacity, negatively affecting bone health. To investigate this, eighty male rats were divided into four groups (n = 20) receiving either nicotine hemisulfate or saline solution (SS) for 28 days. Two daily subcutaneous applications were administered accordingly. Concurrently, vegetable glycerin and Q10 gavage began on day "0". SS: the animals in this group received two daily subcutaneous applications of sodium chloride solution during the entire trial period. 30 days after starting the SS applications subcutaneously, the animals received vegetable glycerin daily until the end of the experiment. SS-Q10: the animals received the SS protocol and daily supplementation with Q10 until the end of the experiment. NIC: The animals received the protocol for NIC and vegetable glycerin daily until the end of the experiment. NIC-Q10: The animals received the protocol for NIC and daily supplementation and Q10 until the end of the experiment. Euthanasia occurred at 7 and 28 days after the beginning the gavage. The tibiae collected were processed for morphometric, densitometric, mechanical, and microtomographic (micro-Ct) analysis. A complementary analysis of intestinal changes was performed. The groups that received Q10 showed slightly better results regarding the mechanical resistance and micro-Ct parameters and to intestinal histomorphometry, as compared with groups not supplemented with Q10. Thus, in rats, it can be concluded that coenzyme Q10 exhibited a protective property to the skeletal system and the gastrointestinal tract, even in the presence of nicotine.

Background/Objectives: Tamoxifen, a selective estrogen receptor modulator widely used as an adjunct in the treatment of breast cancer, has known effects on bone metabolism, although its impact on osseointegration and cellular responses during early bone healing remains unclear. Understanding these effects is essential given the increasing use of dental implants in cancer survivors. The study aimed to observe the influence of tamoxifen on human osteosarcoma (SAOS-2) cells lines, as well on the osseointegration of titanium implants in ovariectomized female rats. Methods: SAOS-2 cells were incubated with Dulbecco’s modified growth medium. Six titanium (Ti) disks were used at each time point. The samples were divided into groups with the presence (TAM, n = 36) or not (CTR, n = 36) of tamoxifen in a concentration of 2 μM. In vivo, 72 animals were divided in groups with bilateral ovariectomy or SHAM and tamoxifen administration or not (15 mg/kg). Cell viability, mineralization rate, and collagen synthesis were assessed, as well as bone/implant contact (BIC) and bone ingrowth (BIN). Results: Tamoxifen caused a decrease in SAOS-2 viability, although an increase in the mineralization rate was observed. In vivo, the TAM groups presented higher BIC and BIN when compared to their control, but a lower percentage of mature collagen cells. Conclusions: Based on our findings, in vitro, the therapy with TAM slightly reduced the viability of SAOS-2 cells while significantly increasing the mineralization rate. In vivo, the therapy positively influenced BIC and BIN during the osseointegration phase.

A recessão gengival é caracterizada como o deslocamento apical da margem gengival em relação à junção cemento-esmalte, com consequente exposição da superfície radicular podendo promover desconforto estético e funcional. O tratamento das recessões gengivais consiste em técnicas cirúrgicas de recobrimento radicular, geralmente associadas ao enxerto de tecido conjuntivo autógeno, contudo, para a sua obtenção existe a necessidade de um segundo sítio cirúrgico, aumentando a morbidade no pós-operatório do paciente. O propósito deste relato de caso é apresentar a matriz de colágeno suína associada ao retalho posicionado coronal como alternativa ao enxerto autógeno de tecido conjuntivo. Paciente do gênero feminino, 45 anos, compareceu ao atendimento na clínica de periodontia do Unifunec (Centro Universitário de Santa Fé do Sul), queixando-se da estética do sorriso devido a recessões gengivais dos dentes 22 a 24. Após a avaliação clínica, a paciente foi diagnosticada com recessão gengival Classe RT1 dos dentes 22 a 24. A paciente foi submetida a cirurgia de recobrimento radicular por meio da técnica do retalho posicionado coronal associado à matriz de colágeno suína. Foi realizado acompanhamento de 30 dias, 12 e 24 meses. Após acompanhamento dos referidos períodos, notou-se um recobrimento satisfatório da superfície radicular, ganho de tecido gengival em altura e espessura evidenciado clinicamente, ausência de recidiva e trazendo satisfação funcional e estética para o paciente. Conclui-se que a utilização da matriz de colágeno suína, reduziu a morbidade do paciente, promoveu ganho de espessura do tecido gengival na área receptora, favorecendo a estabilidade tecidual. PORCINE COLLAGEN MATRIX AND CORONALLY DISPLACED FLAP IN THE TREATMENT OF MULTIPLE RECESSIONS: A CASE REPORT ABSTRACT Gingival recession is characterized as the apical displacement of the gingival margin in relation to the cement enamel junction, with consequent exposure of the root surface, which may cause aesthetic and functional discomfort. The treatment of gingival recessions consists of surgical techniques for root coverage, which are usually associated with autogenous connective tissue grafting; however, in order to obtain it, a second surgical procedure is required, increasing the patient's postoperative morbidity. The aim of this case report is to present the porcine collagen matrix associated with the coronally positioned flap as an alternative to autogenous connective tissue grafting. A 45-year-old female patient came to the periodontics clinic of the Unifunec (University Center of Santa Fé do Sul) complaining about the aesthetics of her smile due to gingival recession on teeth 22 to 24. After clinical evaluation, the patient was diagnosed with Class RT1 gingival recession on teeth 22 to 24. The patient underwent root coverage surgery using the coronally positioned flap technique associated with porcine collagen matrix. Follow-up was performed for 30 days, 12 and 24 months. After follow-up of the mentioned periods, it was noted a satisfactory covering of the root surface, gain of gingival tissue in height and thickness clinically evidenced, absence of relapse and bringing functional and aesthetic satisfaction to the patient. It can be concluded that the use of porcine collagen matrix reduced the morbidity of the patient, promoted thickness gain of the gingival tissue in the recipient area, favoring tissue stability. Keywords: Gingival recession. Periodontics. Gingiva. Soft tissue management. Collagen matrix. Mucogingival surgery.