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Euterpe oleracea Mart. (açai) is a native palm from the Amazon region. There are various chemical constituents of açai with bioactive properties. This study aimed to evaluate the chemical composition and cytotoxic effects of açai seed extract on breast cancer cell line (MCF-7). Global Natural Products Social Molecular Networking (GNPS) was applied to identify chemical compounds present in açai seed extract. LC-MS/MS and molecular networking were employed to detect the phenolic compounds of açai. The antioxidant activity of açai seed extract was measured by DPPH assay. MCF-7 breast cancer cell line viability was evaluated by MTT assay. Cell death was evaluated by flow cytometry and time-lapse microscopy. Autophagy was evaluated by orange acridin immunofluorescence assay. Reactive oxygen species (ROS) production was evaluated by DAF assay. From the molecular networking, fifteen compounds were identified, mainly phenolic compounds. The açai seed extract showed cytotoxic effects against MCF-7, induced morphologic changes in the cell line by autophagy and increased the ROS production pathway. The present study suggests that açai seed extract has a high cytotoxic capacity and may induce autophagy by increasing ROS production in breast cancer. Apart from its antioxidant activity, flavonoids with high radical scavenging activity present in açai also generated NO (nitric oxide), contributing to its cytotoxic effect and autophagy induction.
Marcos Silva; Jonas Costa; Taícia Pacheco-Fill; Ana Ruiz; Flávia Vidal; Kátia Borges; Sulayne Guimarães; Ana Azevedo-Santos; Kaio Buglio; Mary Foglio; Maria Barbosa; Maria Nascimento; João de Carvalho. Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line. Molecules 2021, 26, 3546 .
AMA StyleMarcos Silva, Jonas Costa, Taícia Pacheco-Fill, Ana Ruiz, Flávia Vidal, Kátia Borges, Sulayne Guimarães, Ana Azevedo-Santos, Kaio Buglio, Mary Foglio, Maria Barbosa, Maria Nascimento, João de Carvalho. Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line. Molecules. 2021; 26 (12):3546.
Chicago/Turabian StyleMarcos Silva; Jonas Costa; Taícia Pacheco-Fill; Ana Ruiz; Flávia Vidal; Kátia Borges; Sulayne Guimarães; Ana Azevedo-Santos; Kaio Buglio; Mary Foglio; Maria Barbosa; Maria Nascimento; João de Carvalho. 2021. "Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line." Molecules 26, no. 12: 3546.
Aspergillus fumigatus produces diverse secondary metabolites whose biological functions and regulation remain to be understood. Despite the importance of the conidia for this fungus, the role of the conidia-born metabolite fumiquinazoline C (FqC) is unclear. Here, we describe a dual function of the cell-wall integrity pathway in regulating FqC biosynthesis dictated by the MAPK kinase MpkA, which phosphorylates one of the nonribosomal peptide synthetases enzymes of the cluster (FmqC), and the transcription factor RlmA, which directly regulates the expression of fmq genes. Another level of crosstalk between the FqC regulation and the cell physiology is described since the deletion of the stress-responsive transcription factor sebA provokes derepression of the fmq cluster and overproduction of FqC. Thus, we describe a mechanism by which A. fumigatus controls FqC biosynthesis orchestrated by MpkA-RlmA and SebA and hence enabling survival and adaptation to the environmental niche, given that FqC is a deterrent of ameba predation.
Marina Campos Rocha; João Henrique Tadini Marilhano Fabri; Lilian Pereira da Silva; Célio Fernando Figueiredo Angolini; Maria Célia Bertolini; Anderson Ferreira da Cunha; Vito Valiante; Gustavo Henrique Goldman; Taicia Pacheco Fill; Iran Malavazi. Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection. Genetics 2021, 218, 1 .
AMA StyleMarina Campos Rocha, João Henrique Tadini Marilhano Fabri, Lilian Pereira da Silva, Célio Fernando Figueiredo Angolini, Maria Célia Bertolini, Anderson Ferreira da Cunha, Vito Valiante, Gustavo Henrique Goldman, Taicia Pacheco Fill, Iran Malavazi. Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection. Genetics. 2021; 218 (1):1.
Chicago/Turabian StyleMarina Campos Rocha; João Henrique Tadini Marilhano Fabri; Lilian Pereira da Silva; Célio Fernando Figueiredo Angolini; Maria Célia Bertolini; Anderson Ferreira da Cunha; Vito Valiante; Gustavo Henrique Goldman; Taicia Pacheco Fill; Iran Malavazi. 2021. "Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection." Genetics 218, no. 1: 1.
Penicillium digitatum is the most aggressive pathogen of citrus fruits. Tryptoquialanines are major indole alkaloids produced by P. digitatum. It is unknown if tryptoquialanines are involved in the damage of citrus fruits caused by P. digitatum. To investigate the pathogenic roles of tryptoquialanines, we initially asked if tryptoquialanines could affect the germination of Citrus sinensis seeds. Exposure of the citrus seeds to tryptoquialanine A resulted in a complete inhibition of germination and an altered metabolic response. Since this phytotoxic effect requires the extracellular export of tryptoquialanine A, we investigated the mechanisms of extracellular delivery of this alkaloid in P. digitatum. We detected extracellular vesicles (EVs) released by P. digitatum both in culture and during infection of citrus fruits. Compositional analysis of EVs produced during infection revealed the presence of a complex cargo, which included tryptoquialanines and the mycotoxin fungisporin. The EVs also presented phytotoxicity activity in vitro, and caused damage to the tissues of citrus seeds. Through molecular networking, it was observed that the metabolites present in the P. digitatum EVs are produced in all of its possible hosts. Our results reveal a novel phytopathogenic role of P. digitatum EVs and tryptoquialanine A, implying that this alkaloid is exported in EVs during plant infection. IMPORTANCE During the post-harvest period, citrus fruits can be affected by phytopathogens such as Penicillium digitatum, which causes the green mold disease and is responsible for up to 90 % of the total citrus losses. Chemical fungicides are widely used to prevent the green mold disease, leading to concerns about environmental and health risks. To develop safer alternatives to control phytopathogens, it is necessary to understand the molecular basis of infection during the host-pathogen interaction. In the P. digitatum model, the virulence strategies are poorly known. Here, we describe the production of phytotoxic extracellular vesicles (EVs) by P. digitatum during the infection of citrus fruits. We also characterized the secondary metabolites in the cargo of EVs and found in this set of molecules an inhibitor of seed germination. Since EVs and secondary metabolites have been related to virulence mechanisms in other host-pathogen interactions, our data are important for the comprehension of how P. digitatum causes damage to its primary hosts.
Jonas Henrique Costa; Jaqueline Moraes Bazioli; Luidy Darllan Barbosa; Pedro Luis Theodoro Dos Santos Júnior; Flavia C. G. Reis; Tabata Klimeck; Camila Manoel Crnkovic; Roberto G. S. Berlinck; Alessandra Sussulini; Marcio L. Rodrigues; Taícia Pacheco Fill. Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles. 2020, 1 .
AMA StyleJonas Henrique Costa, Jaqueline Moraes Bazioli, Luidy Darllan Barbosa, Pedro Luis Theodoro Dos Santos Júnior, Flavia C. G. Reis, Tabata Klimeck, Camila Manoel Crnkovic, Roberto G. S. Berlinck, Alessandra Sussulini, Marcio L. Rodrigues, Taícia Pacheco Fill. Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles. . 2020; ():1.
Chicago/Turabian StyleJonas Henrique Costa; Jaqueline Moraes Bazioli; Luidy Darllan Barbosa; Pedro Luis Theodoro Dos Santos Júnior; Flavia C. G. Reis; Tabata Klimeck; Camila Manoel Crnkovic; Roberto G. S. Berlinck; Alessandra Sussulini; Marcio L. Rodrigues; Taícia Pacheco Fill. 2020. "Phytotoxic tryptoquialanines produced in vivo by Penicillium digitatum are exported in extracellular vesicles." , no. : 1.
A. fumigatus is the main etiological agent of invasive pulmonary aspergillosis, a life-threatening fungal disease that occurs in severely immunocompromised humans. Withstanding the host environment is essential for A. fumigatus virulence, and sensing of extracellular cues occurs primarily through G-protein coupled receptors (GPCRs) that activate signal transduction pathways, which, in turn, regulate fungal development, metabolism, virulence, and mycotoxin biosynthesis. The A. fumigatus genome encodes 15 putative classical GPCRs, with only three having been functionally characterized to date. In this work, we show that the two GPCRs GprM and GprJ regulate the phosphorylation of the mitogen-activated protein kinase MpkA and thus control the regulation of the cell wall integrity pathway. GprM and GprJ are also involved in the regulation of the production of the secondary metabolites fumagillin, pyripyropene, fumigaclavine C, fumiquinazoline, melanin, and fumitremorgin, and this regulation partially occurs through the activation of MpkA. Furthermore, GprM and GprJ are important for virulence in the insect model Galleria mellonella . This work therefore functionally characterizes two GPCRs and shows how they regulate several intracellular pathways that have been shown to be crucial for A. fumigatus virulence.
Aílton Pereira Da Costa Filho; Guilherme Thomaz Pereira Brancini; Patrícia Alves de Castro; Clara Valero; Jaire Alves Ferreira Filho; Lilian Pereira Silva; Marina Campos Rocha; Iran Malavazi; João Guilherme De Moraes Pontes; Taícia Fill; Roberto Nascimento Silva; Fausto Almeida; Jacob L. Steenwyk; Antonis Rokas; Thaila F. dos Reis; Laure N. A. Ries; Gustavo H. Goldman. Aspergillus fumigatus G-Protein Coupled Receptors GprM and GprJ Are Important for the Regulation of the Cell Wall Integrity Pathway, Secondary Metabolite Production, and Virulence. mBio 2020, 11, 1 .
AMA StyleAílton Pereira Da Costa Filho, Guilherme Thomaz Pereira Brancini, Patrícia Alves de Castro, Clara Valero, Jaire Alves Ferreira Filho, Lilian Pereira Silva, Marina Campos Rocha, Iran Malavazi, João Guilherme De Moraes Pontes, Taícia Fill, Roberto Nascimento Silva, Fausto Almeida, Jacob L. Steenwyk, Antonis Rokas, Thaila F. dos Reis, Laure N. A. Ries, Gustavo H. Goldman. Aspergillus fumigatus G-Protein Coupled Receptors GprM and GprJ Are Important for the Regulation of the Cell Wall Integrity Pathway, Secondary Metabolite Production, and Virulence. mBio. 2020; 11 (5):1.
Chicago/Turabian StyleAílton Pereira Da Costa Filho; Guilherme Thomaz Pereira Brancini; Patrícia Alves de Castro; Clara Valero; Jaire Alves Ferreira Filho; Lilian Pereira Silva; Marina Campos Rocha; Iran Malavazi; João Guilherme De Moraes Pontes; Taícia Fill; Roberto Nascimento Silva; Fausto Almeida; Jacob L. Steenwyk; Antonis Rokas; Thaila F. dos Reis; Laure N. A. Ries; Gustavo H. Goldman. 2020. "Aspergillus fumigatus G-Protein Coupled Receptors GprM and GprJ Are Important for the Regulation of the Cell Wall Integrity Pathway, Secondary Metabolite Production, and Virulence." mBio 11, no. 5: 1.
The unexplored saline lagoons of the north of Peru harbor a rich microbiome, due to reported studies of different extreme environments around the world. In these regions, there are several ecosystems and microhabitats not yet explored, and little is known about the diversity of actinobacteria and other microorganisms. We suggest that the endemic bacteria present in this extreme environment could be source of active molecules with anticancer, antimicrobial, antiparasitic properties. Using phenotypic and genotypic characterization techniques including the 16S rRNA were identified into the genera Streptomyces 39 (78%), Pseudonocardia 3 (6%), Staphylococcus 4 (8%), Bacillus 2 (4%), and Pseudomonas 2 (4%). All isolated bacteria for the genotypic data were preliminarily identified. Actinobacteria strains were found dominantly in both sites (Lagoon1-3 = 16 isolates and lagoon 4 = 12 isolates). Phylogenetic analysis revealed that 28 isolates were exclusively affiliated to eleven different clusters of Actinobacteria of the major genus Streptomyces. Three Streptomyces sp. strains M-92, B-146, and B-81, were tested for antibacterial and antiproliferative activities. The results showed antiproliferative activities against three tumor cell lines, U251 glioma; MCF7 breast; NCI-H460 lung non-small type of cells, and the antibacterial activity to Staphylococcus aureus ATCC 6538, E. coli ATCC 10536, and Acinetobacter baumanni AC-972 which is resistant to multiple drugs. The promising results belong to Streptomyces sp. B-81 strain in the R2A medium using a doxorubicin with control positive, the best result was from the latter (TGI = 0,57 µg/mL) for glioma; NCI-H460 lung of type non-small cells (TGI = 0,61 µg/mL), and breast cancer (TGI =0,80 µg/mL), this strain was selected to be fractionated because it had better antiproliferative and antibacterial activity, and its fractions were evaluated concerning antiproliferative activity against nine types of tumor cells and one non-tumor. The methanolic fraction showed a better result in the antiproliferative activity and was able to inhibit U251 (glioma) (TGI = 38.3 µg/mL), OVCAR-03 (ovary) (TGI = 62.1 µg/mL), and K562 (leukemia) (TGI = 81.5 µg/mL). The methanol 50% - acetate 50% fraction (Fraction 4) inhibited U251 (glioma) (TGI = 73.5 µg/mL) and UACC-62 (melanoma) (TGI = 89.4 µg/mL). Moreover, the UHPLC-MS/MS data and molecular networking of Streptomyces sp. B-81 isolate extract revealed the production cholic acid, Lobophorin A, Lobophorin B, Lobophorin E, Lobophorin K and compound 6. Extremophilic environments such as the Mórrope and Bayovar Salt Flats are promising sources of new bacteria with promising pharmaceutical potential; These compounds could be useful to treat various infectious diseases or even some type of cancer.
Rene Flores Clavo; Nataly Ruiz Quiñones; Alvaro Tasca Hernandez; Ana Lucia Tasca Gois Ruiz; Lucia Elaine De Oliveira Braga; Zhandra Lizeth Arce Gil; Luis Miguel Serquen Lopez; Jonas Henrique Costa; Taícia Pacheco Fill; Marcos José Salvador; Fabiana Fantinatti Garboggini. EVALUATION OF ANTIMICROBIAL AND ANTIPROLIFERATIVE ACTIVITIES OF ACTINOBACTERIA ISOLATED FROM THE SALINE LAGOONS OF NORTHWEST PERU. 2020, 1 .
AMA StyleRene Flores Clavo, Nataly Ruiz Quiñones, Alvaro Tasca Hernandez, Ana Lucia Tasca Gois Ruiz, Lucia Elaine De Oliveira Braga, Zhandra Lizeth Arce Gil, Luis Miguel Serquen Lopez, Jonas Henrique Costa, Taícia Pacheco Fill, Marcos José Salvador, Fabiana Fantinatti Garboggini. EVALUATION OF ANTIMICROBIAL AND ANTIPROLIFERATIVE ACTIVITIES OF ACTINOBACTERIA ISOLATED FROM THE SALINE LAGOONS OF NORTHWEST PERU. . 2020; ():1.
Chicago/Turabian StyleRene Flores Clavo; Nataly Ruiz Quiñones; Alvaro Tasca Hernandez; Ana Lucia Tasca Gois Ruiz; Lucia Elaine De Oliveira Braga; Zhandra Lizeth Arce Gil; Luis Miguel Serquen Lopez; Jonas Henrique Costa; Taícia Pacheco Fill; Marcos José Salvador; Fabiana Fantinatti Garboggini. 2020. "EVALUATION OF ANTIMICROBIAL AND ANTIPROLIFERATIVE ACTIVITIES OF ACTINOBACTERIA ISOLATED FROM THE SALINE LAGOONS OF NORTHWEST PERU." , no. : 1.
Guanidine metabolites isolated from microorganisms, marine invertebrates, terrestrial animals and plants are reviewed, as well as the biosynthesis, total synthesis, biological activities and ecological roles of guanidine natural products.
Roberto G. S. Berlinck; Darlon I. Bernardi; Taicia Fill; Alessandra A. G. Fernandes; Igor D. Jurberg. The chemistry and biology of guanidine secondary metabolites. Natural Product Reports 2020, 38, 586 -667.
AMA StyleRoberto G. S. Berlinck, Darlon I. Bernardi, Taicia Fill, Alessandra A. G. Fernandes, Igor D. Jurberg. The chemistry and biology of guanidine secondary metabolites. Natural Product Reports. 2020; 38 (3):586-667.
Chicago/Turabian StyleRoberto G. S. Berlinck; Darlon I. Bernardi; Taicia Fill; Alessandra A. G. Fernandes; Igor D. Jurberg. 2020. "The chemistry and biology of guanidine secondary metabolites." Natural Product Reports 38, no. 3: 586-667.
G-protein coupled receptors (GPCRs) are extracellular signalling receptors that sense environmental cues to coordinate a biological response. Fungi sense their environment primarily through GPCR-mediated signalling pathways, which in turn regulate fungal development, metabolism, virulence and mycotoxin biosynthesis. A. fumigatus is an important human pathogen that causes aspergillosis, a heterogeneous group of diseases that presents a wide range of clinical manifestations. Here, we investigate in detail the role of the GPCRs GprM and GprJ in growth and gene expression. GprM and GprJ are important for melanin production and the regulation of the cell wall integrity (CWI) pathway. Overexpression of gprM and gprJ causes a 20 and 50% reduction in growth rate when compared to the wild-type (WT) strain, and increases sensitivity to cell wall-damaging agents. Phosphorylation of the CWI protein kinase MpkA is increased in the ΔgprM and ΔgprJ strains and decreased in the overexpression mutants when compared to the WT strain. Furthermore, differences in cell wall polysaccharide concentrations and organization were observed in these strains. RNA-sequencing suggests that GprM and GprJ negatively regulate genes encoding secondary metabolites (SMs). Mass spectrometry analysis confirmed that the production of fumagillin, pyripyropene, fumigaclavine C, fumiquinazoline, and fumitremorgin is reduced in the ΔgprM and ΔgprJ strains, and that this regulation occurs, at least partially, through the activation of MpkA. Overexpression of grpM also resulted in the regulation of many transcription factors, with AsgA predicted to function downstream of GprM and MpkA signalling. Finally, we show that the ΔgprM and ΔgprJ mutants are reduced in virulence in the Galleria mellonella insect model of invasive aspergillosis. This work further contributes to unravelling functions of A. fumigatus GPCRs and shows that GprM and GprJ are essential for CWI, secondary metabolite production and virulence. Author summary A. fumigatus is the main ethiological agent of invasive pulmonary aspergillosis, a life-threatening fungal disease that occurs in severely immuno-compromised humans. Withstanding the host environment is essential for A. fumigatus virulence and sensing of extracellular cues occurs primarily through G-protein coupled receptors (GPCRs) that activate signal transduction pathways, which in turn regulate fungal development, metabolism, virulence and mycotoxin biosynthesis. The A. fumigatus genome encodes 15 putative classical GPCRs, with only three having been functionally characterized to date. In this work, we show that the two GPCRs GprM and GprJ regulate the phosphorylation of the mitogen-activated protein kinase MpkA and thus control the regulation of the cell wall integrity pathway. GprM and GprJ are also involved in the regulation of the production of the secondary metabolites fumagillin, pyripyropene, fumigaclavine C, fumiquinazoline, melanin, and fumitremorgin and this regulation partially occurs through the activation of MpkA. Furthermore, GprM and GprJ are important for virulence in the insect model Galleria mellonella. This work therefore functionally characterizes two GPCRs and shows how they regulate several intracellular pathways that have been shown to be crucial for A. fumigatus virulence.
Aílton Pereira Da Costa Filho; Guilherme Thomaz Pereira Brancini; Patrícia Alves de Castro; Jaire Alves Ferreira; Lilian Pereira Silva; Marina Campos Rocha; Iran Malavazi; João Guilherme De Moraes Pontes; Taícia Fill; Roberto Nascimento Silva; Fausto Almeida; Jacob L. Steenwyk; Antonis Rokas; Thaila F. dos Reis; Laure N.A. Ries; Gustavo H. Goldman. Aspergillus fumigatus G-protein coupled receptors GprM and GprJ are important for the regulation of the cell wall integrity pathway, secondary metabolite production, and virulence. 2020, 1 .
AMA StyleAílton Pereira Da Costa Filho, Guilherme Thomaz Pereira Brancini, Patrícia Alves de Castro, Jaire Alves Ferreira, Lilian Pereira Silva, Marina Campos Rocha, Iran Malavazi, João Guilherme De Moraes Pontes, Taícia Fill, Roberto Nascimento Silva, Fausto Almeida, Jacob L. Steenwyk, Antonis Rokas, Thaila F. dos Reis, Laure N.A. Ries, Gustavo H. Goldman. Aspergillus fumigatus G-protein coupled receptors GprM and GprJ are important for the regulation of the cell wall integrity pathway, secondary metabolite production, and virulence. . 2020; ():1.
Chicago/Turabian StyleAílton Pereira Da Costa Filho; Guilherme Thomaz Pereira Brancini; Patrícia Alves de Castro; Jaire Alves Ferreira; Lilian Pereira Silva; Marina Campos Rocha; Iran Malavazi; João Guilherme De Moraes Pontes; Taícia Fill; Roberto Nascimento Silva; Fausto Almeida; Jacob L. Steenwyk; Antonis Rokas; Thaila F. dos Reis; Laure N.A. Ries; Gustavo H. Goldman. 2020. "Aspergillus fumigatus G-protein coupled receptors GprM and GprJ are important for the regulation of the cell wall integrity pathway, secondary metabolite production, and virulence." , no. : 1.
Phytopathogens are responsible for great losses in agriculture, once they are able to subvert or elude the host defense mechanisms through virulence factors secretion for their dissemination. Herein, it is reviewed phytotoxins that act as virulence factors and are produced by bacterial phytopathogens (Candidatus Liberibacter spp., Erwinia amylovora, Pseudomonas syringae pvs and Xanthomonas spp.) and fungi (Alternaria alternata, Botrytis cinerea, Cochliobolus spp., Fusarium spp., Magnaporthe spp., and Penicillium spp.), which were selected in accordance to their worldwide importance due to the biochemical and economical aspects. In the current review, it is sought to understand the role of virulence factors in the pathogen–host interactions that result in plant diseases.
João Guilherme De Moraes Pontes; Laura Soler Fernandes; Roney Vander dos Santos; Ljubica Tasic; Taicia Pacheco Fill. Virulence Factors in the Phytopathogen–Host Interactions: An Overview. Journal of Agricultural and Food Chemistry 2020, 68, 7555 -7570.
AMA StyleJoão Guilherme De Moraes Pontes, Laura Soler Fernandes, Roney Vander dos Santos, Ljubica Tasic, Taicia Pacheco Fill. Virulence Factors in the Phytopathogen–Host Interactions: An Overview. Journal of Agricultural and Food Chemistry. 2020; 68 (29):7555-7570.
Chicago/Turabian StyleJoão Guilherme De Moraes Pontes; Laura Soler Fernandes; Roney Vander dos Santos; Ljubica Tasic; Taicia Pacheco Fill. 2020. "Virulence Factors in the Phytopathogen–Host Interactions: An Overview." Journal of Agricultural and Food Chemistry 68, no. 29: 7555-7570.
Most of the biosynthetic gene clusters (BGCs) found in filamentous fungi are silent under standard laboratory cultivation conditions due to the lack of expression triggering stimuli, representing a considerable drawback in drug discovery. To access the full biosynthetic potential of these microbes, studies towards the activation of cryptic BGCs are essential. Histone acetylation status is an important regulator of chromatin structure which impacts in cell physiology and, therefore, expression of biosynthetic gene clusters in filamentous fungi. Histone deacetylases (HDACs) and histone acetyl-transferases (HATs) are responsible for maintaining and controlling this process under different cell conditions. In this study, clr3, a gene encoding a histone deacetylase in Penicillium brasilianum was deleted and associated phenotypic and metabolic changes evaluated. Results indicate reduced growth under oxidative stress conditions in the Δclr3 knockout strain. Also, the production of several secondary metabolites including austin-related meroterpenoids, brasiliamides, mycotoxins such as verruculogen and penicillic acid, as well as cyclodepsipeptides was reduced in the Δclr3 strain when compared to wild-type strain. Accordingly, addition of epigenetic modulators responsible for HDAC inhibition such as suberoylanilide hydroxamic acid (SAHA) and nicotinamide (NAA) to P. brasilianum growth media also culminated in reduction of secondary metabolite production. Mass Spectrometry Imaging (MSI) was applied to compare metabolite production and spatial distribution on the colony. Results suggest that Clr3 plays an important role in secondary metabolite biosynthesis in P. brasilianum, thus offering new strategies for regulation of natural product synthesis by assessing chromatin modification in P. brasilianum.
Daniel Yuri Akiyama; Marina Campos Rocha; Jonas Henrique Costa; Iran Malavazi; Taicia Pacheco Fill. The histone deacetylase clr3 regulates secondary metabolite production and growth under oxidative stress conditions in Penicillium brasilianum. 2020, 1 .
AMA StyleDaniel Yuri Akiyama, Marina Campos Rocha, Jonas Henrique Costa, Iran Malavazi, Taicia Pacheco Fill. The histone deacetylase clr3 regulates secondary metabolite production and growth under oxidative stress conditions in Penicillium brasilianum. . 2020; ():1.
Chicago/Turabian StyleDaniel Yuri Akiyama; Marina Campos Rocha; Jonas Henrique Costa; Iran Malavazi; Taicia Pacheco Fill. 2020. "The histone deacetylase clr3 regulates secondary metabolite production and growth under oxidative stress conditions in Penicillium brasilianum." , no. : 1.
Citrus are vulnerable to the postharvest decay caused by Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii, which are responsible for the green mold, blue mold, and sour rot post-harvest disease, respectively. The widespread economic losses in citriculture caused by these phytopathogens are minimized with the use of synthetic fungicides such as imazalil, thiabendazole, pyrimethanil, and fludioxonil, which are mainly employed as control agents and may have harmful effects on human health and environment. To date, numerous non-chemical postharvest treatments have been investigated for the control of these pathogens. Several studies demonstrated that biological control using microbial antagonists and natural products can be effective in controlling postharvest diseases in citrus, as well as the most used commercial fungicides. Therefore, microbial agents represent a considerably safer and low toxicity alternative to synthetic fungicides. In the present review, these biological control strategies as alternative to the chemical fungicides are summarized here and new challenges regarding the development of shelf-stable formulated biocontrol products are also discussed.
Jaqueline Moraes Bazioli; João Raul Belinato; Jonas Henrique Costa; Daniel Yuri Akiyama; João Guilherme De Moraes Pontes; Katia Cristina Kupper; Fabio Augusto; João Ernesto De Carvalho; Taícia Pacheco Fill. Biological Control of Citrus Postharvest Phytopathogens. Toxins 2019, 11, 460 .
AMA StyleJaqueline Moraes Bazioli, João Raul Belinato, Jonas Henrique Costa, Daniel Yuri Akiyama, João Guilherme De Moraes Pontes, Katia Cristina Kupper, Fabio Augusto, João Ernesto De Carvalho, Taícia Pacheco Fill. Biological Control of Citrus Postharvest Phytopathogens. Toxins. 2019; 11 (8):460.
Chicago/Turabian StyleJaqueline Moraes Bazioli; João Raul Belinato; Jonas Henrique Costa; Daniel Yuri Akiyama; João Guilherme De Moraes Pontes; Katia Cristina Kupper; Fabio Augusto; João Ernesto De Carvalho; Taícia Pacheco Fill. 2019. "Biological Control of Citrus Postharvest Phytopathogens." Toxins 11, no. 8: 460.
Penicillium digitatum is the major source of postharvest decay in citrus fruits worldwide. This fungus shows a notably limited host range, being able to infect mainly mature fruit belonging to the Rutaceae family. This highly specific host interaction has attracted the interest of the scientific community. Researchers have investigated the chemical interactions and specialized virulence strategies that facilitate this fungus’s fruit colonization, thereby leading to a successful citrus infection. There are several factors that mediate and affect the interaction between Penicillium digitatum and its host citrus, including hydrogen peroxide modulation by the phytopathogen, secretion of organic acids and consequently pH control, and other strategies described in this review. The recently achieved sequencing of the complete Penicillium digitatum genome opened up new possibilities for exploration of the virulence factors related to the host-pathogen interaction. Through such techniques as RNAseq, RT-PCR and targeted gene knockout mediated by Agrobacterium tumefaciens, important genes involved in the fungal infection process in citrus have been reported, helping to elucidate the molecular mechanisms, metabolites and genetic components that are involved in the pathogenicity of P. digitatum. Understanding the infection process and fungal strategies represents an important step in developing ways to protect citrus from Penicillium digitatum infection, possibly leading to more productive citriculture worldwide.
Jonas Henrique Costa; Jaqueline Moraes Bazioli; João Guilherme De Moraes Pontes; Taícia Pacheco Fill. Penicillium digitatum infection mechanisms in citrus: What do we know so far? Fungal Biology 2019, 123, 584 -593.
AMA StyleJonas Henrique Costa, Jaqueline Moraes Bazioli, João Guilherme De Moraes Pontes, Taícia Pacheco Fill. Penicillium digitatum infection mechanisms in citrus: What do we know so far? Fungal Biology. 2019; 123 (8):584-593.
Chicago/Turabian StyleJonas Henrique Costa; Jaqueline Moraes Bazioli; João Guilherme De Moraes Pontes; Taícia Pacheco Fill. 2019. "Penicillium digitatum infection mechanisms in citrus: What do we know so far?" Fungal Biology 123, no. 8: 584-593.
Penicillium brasilianum (strain LaBioMMi 136) has been reported to be a great producer of secondary metabolites and a source of enzymes of biotechnological interest. Here, we report the draft genome sequence of P. brasilianum (strain LaBioMMi 136), isolated as an endophyte from the plant Melia azedarach (family Meliaceae).
Taícia Pacheco Fill; Jéssica Fernanda Baretta; Luiz Almeida; Iran Malavazi; Louise Cerdeira; Markiyan Samborskyy; Ana Tereza Ribeiro de Vasconcelos; Peter Leadlay; Edson Rodrigues-Filho. Draft Genome Sequence of the Fungus Penicillium brasilianum (Strain LaBioMMi 136), a Plant Endophyte from Melia azedarach. Microbiology Resource Announcements 2018, 7, e01235-18 .
AMA StyleTaícia Pacheco Fill, Jéssica Fernanda Baretta, Luiz Almeida, Iran Malavazi, Louise Cerdeira, Markiyan Samborskyy, Ana Tereza Ribeiro de Vasconcelos, Peter Leadlay, Edson Rodrigues-Filho. Draft Genome Sequence of the Fungus Penicillium brasilianum (Strain LaBioMMi 136), a Plant Endophyte from Melia azedarach. Microbiology Resource Announcements. 2018; 7 (21):e01235-18.
Chicago/Turabian StyleTaícia Pacheco Fill; Jéssica Fernanda Baretta; Luiz Almeida; Iran Malavazi; Louise Cerdeira; Markiyan Samborskyy; Ana Tereza Ribeiro de Vasconcelos; Peter Leadlay; Edson Rodrigues-Filho. 2018. "Draft Genome Sequence of the Fungus Penicillium brasilianum (Strain LaBioMMi 136), a Plant Endophyte from Melia azedarach." Microbiology Resource Announcements 7, no. 21: e01235-18.
In this study, the consumption of 4-bromobenzoic acid and 4-chlorobenzoic acid by the fungus Penicillium brasilianum, an endophyte from Melia azedarach is evaluated. This fungus metabolizes these halobenzoic acids to produce three new brominated compounds, which have been isolated and characterized, and three new chlorinated derivatives identified by HRMS. Among these products, (4-bromobenzoyl)proline has been also chemically synthesized and employed in biological assays, thus providing insights for the elucidation of the defense mechanism of P. brasilianum towards these halobenzoic acids.
Taícia Pacheco Fill; Heloisa Fassina Pallini; Zia Ud Din; Igor Dias Jurberg; José Vinicius da Silva; Edson Rodrigues-Filho. Conjugation of antifungal benzoic acid derivatives as a path for detoxification in Penicillium brasilianum, an endophyte from Melia azedarach. Bioorganic Chemistry 2018, 81, 367 -372.
AMA StyleTaícia Pacheco Fill, Heloisa Fassina Pallini, Zia Ud Din, Igor Dias Jurberg, José Vinicius da Silva, Edson Rodrigues-Filho. Conjugation of antifungal benzoic acid derivatives as a path for detoxification in Penicillium brasilianum, an endophyte from Melia azedarach. Bioorganic Chemistry. 2018; 81 ():367-372.
Chicago/Turabian StyleTaícia Pacheco Fill; Heloisa Fassina Pallini; Zia Ud Din; Igor Dias Jurberg; José Vinicius da Silva; Edson Rodrigues-Filho. 2018. "Conjugation of antifungal benzoic acid derivatives as a path for detoxification in Penicillium brasilianum, an endophyte from Melia azedarach." Bioorganic Chemistry 81, no. : 367-372.
Over the past few years Penicillium brasilianum has been isolated from many different environmental sources as soil isolates, plant endophytes and onion pathogen. All investigated strains share a great ability to produce bioactive secondary metabolites. Different authors have investigated this great capability and here we summarize the metabolic potential and the biological activities related to P. brasilianum’s metabolites with diverse structures. They include secondary metabolites of an alkaloid nature, i.e., 2,5-diketopiperazines, cyclodepsipeptides, meroterpenoids and polyketides. Penicillium brasilianum is also described as a great source of enzymes with biotechnological application potential, which is also highlighted in this review. Additionally, this review will focus on several aspects of Penicillium brasilianum and interesting genomic insights.
Jaqueline Moraes Bazioli; Luciana Da Silva Amaral; Taícia Pacheco Fill; Edson Rodrigues-Filho. Insights into Penicillium brasilianum Secondary Metabolism and Its Biotechnological Potential. Molecules 2017, 22, 858 .
AMA StyleJaqueline Moraes Bazioli, Luciana Da Silva Amaral, Taícia Pacheco Fill, Edson Rodrigues-Filho. Insights into Penicillium brasilianum Secondary Metabolism and Its Biotechnological Potential. Molecules. 2017; 22 (6):858.
Chicago/Turabian StyleJaqueline Moraes Bazioli; Luciana Da Silva Amaral; Taícia Pacheco Fill; Edson Rodrigues-Filho. 2017. "Insights into Penicillium brasilianum Secondary Metabolism and Its Biotechnological Potential." Molecules 22, no. 6: 858.
The fungus Xylaria arbuscula was isolated as an endophyte from Cupressus lusitanica and has shown to be a prominent producer of cytochalasans, mainly cytochalasin C, D and Q. Cytochalasans comprise an important class of fungal secondary metabolites that have aroused attention due to their uncommon molecular structures and pronounced biological activities. Due to the few published studies on the ESI-MS/MS fragmentation of this important class of secondary metabolites, in the first part of our work we studied the cytochalasin D fragmentation pathways using an ESI-Q-ToF mass spectrometer coupled with liquid chromatography. We verified that the mainly fragmentation routes were generated by hydrogen and McLafferty rearrangements which provided more ions than just the ones related to the losses of H2O and CO as reported in previous studies. We also confirmed the diagnostic ions at m/z 146 and 120 as direct precursor derived from phenylalanine. The present work also aimed the production of structurally diverse cytochalasans by varying the culture conditions used to grow the fungus X. arbuscula and further insights into the biosynthesis of cytochalasins. HPLC-MS analysis revealed no significant changes in the metabolic profile of the microorganism with the supplementation of different nitrogen sources, but indicated the ability of X. arbuscula to have access to inorganic and organic nitrogen, such as nitrate, ammonium and amino acids as a primary source of nitrogen. The administration of 2-13C glycine showed the direct correlation of this amino acid catabolism and the biosynthesis of cytochalasin D by X. arbuscula, due to the incorporation of three labeled carbons in cytochalasin chemical structure.
Luciana Silva Amaral; Taicia Pacheco Fill; L. F. A. Santos; E. Rodrigues-Filho. Biosynthesis and mass spectral fragmentation pathways of13C and15N labeled cytochalasin D produced byXylaria arbuscula. Journal of Mass Spectrometry 2017, 52, 239 -247.
AMA StyleLuciana Silva Amaral, Taicia Pacheco Fill, L. F. A. Santos, E. Rodrigues-Filho. Biosynthesis and mass spectral fragmentation pathways of13C and15N labeled cytochalasin D produced byXylaria arbuscula. Journal of Mass Spectrometry. 2017; 52 (4):239-247.
Chicago/Turabian StyleLuciana Silva Amaral; Taicia Pacheco Fill; L. F. A. Santos; E. Rodrigues-Filho. 2017. "Biosynthesis and mass spectral fragmentation pathways of13C and15N labeled cytochalasin D produced byXylaria arbuscula." Journal of Mass Spectrometry 52, no. 4: 239-247.
Francisco De Assis Avelino De F. Sobrinho; Francisco Willian De Souza Lucas; Taicia Pacheco Fill; Edson Rodrigues-Filho; Lucia Helena Mascaro; Paulo Naftali Da Silva Casciano; Pedro De Lima-Neto; Adriana Nunes Correia. Insights into electrodegradation mechanism of tebuconazole pesticide on Bi-doped PbO 2 electrodes. Electrochimica Acta 2015, 154, 278 -286.
AMA StyleFrancisco De Assis Avelino De F. Sobrinho, Francisco Willian De Souza Lucas, Taicia Pacheco Fill, Edson Rodrigues-Filho, Lucia Helena Mascaro, Paulo Naftali Da Silva Casciano, Pedro De Lima-Neto, Adriana Nunes Correia. Insights into electrodegradation mechanism of tebuconazole pesticide on Bi-doped PbO 2 electrodes. Electrochimica Acta. 2015; 154 ():278-286.
Chicago/Turabian StyleFrancisco De Assis Avelino De F. Sobrinho; Francisco Willian De Souza Lucas; Taicia Pacheco Fill; Edson Rodrigues-Filho; Lucia Helena Mascaro; Paulo Naftali Da Silva Casciano; Pedro De Lima-Neto; Adriana Nunes Correia. 2015. "Insights into electrodegradation mechanism of tebuconazole pesticide on Bi-doped PbO 2 electrodes." Electrochimica Acta 154, no. : 278-286.
In this work the synthesis and antiparasitical activity of new 1,5-diaryl-3-oxo-1,4-pentadienyl derivatives are described. First, compounds 1a, 1b, 1c and 1d were prepared by acid-catalyzed aldol reaction between 2-butanone and benzaldehyde, anisaldehyde, p-N,N-dimethylaminobenzaldehyde and p-nitrobenzaldehyde. Reacting each of the methyl ketones 1a, 1b, 1c and 1d with the p-substituted benzaldehydes under basic-catalyzed aldol reaction, we further prepared compounds 2a–2p. All twenty compounds were evaluated for antiproliferative activity, particularly for promastigote of Leishmania amazonensis and epimastigote of Trypanosoma cruzi. All compounds showed good activity while nitro compounds 2i and 2k showed inhibition activity at a few μM
Zia Ud Din; Taicia Pacheco Fill; Francisco Favaro de Assis; Danielle Lazarin-Bidóia; Vanessa Kaplum; Francielle Pelegrin Garcia; Celso Vataru Nakamura; Kleber Thiago de Oliveira; Edson Rodrigues-Filho. Unsymmetrical 1,5-diaryl-3-oxo-1,4-pentadienyls and their evaluation as antiparasitic agents. Bioorganic & Medicinal Chemistry 2014, 22, 1121 -1127.
AMA StyleZia Ud Din, Taicia Pacheco Fill, Francisco Favaro de Assis, Danielle Lazarin-Bidóia, Vanessa Kaplum, Francielle Pelegrin Garcia, Celso Vataru Nakamura, Kleber Thiago de Oliveira, Edson Rodrigues-Filho. Unsymmetrical 1,5-diaryl-3-oxo-1,4-pentadienyls and their evaluation as antiparasitic agents. Bioorganic & Medicinal Chemistry. 2014; 22 (3):1121-1127.
Chicago/Turabian StyleZia Ud Din; Taicia Pacheco Fill; Francisco Favaro de Assis; Danielle Lazarin-Bidóia; Vanessa Kaplum; Francielle Pelegrin Garcia; Celso Vataru Nakamura; Kleber Thiago de Oliveira; Edson Rodrigues-Filho. 2014. "Unsymmetrical 1,5-diaryl-3-oxo-1,4-pentadienyls and their evaluation as antiparasitic agents." Bioorganic & Medicinal Chemistry 22, no. 3: 1121-1127.
During our studies concerning endophytic fungi, two indole alkaloids were co-produced with verruculogen by Penicillium brasilianum isolated from Melia azedarach (Meliaceae). The compounds were isolated by the use of combined chromatographic procedures and identified by physical methods, mainly 1D- and 2D-NMR experiments. This article also describes the production of verruculogen TR-2, first described for this species of Penicillium, and a verruculogen TR-2C-11 epimer, that is a novel fungal natural product. The kinetic production of verruculogen and verruculogen TR-2 produced by P. brasilianum were evaluated in order to understand the involvement of verruculogen TR-2 in verruculogen biosynthesis.
Taicia Pacheco Fill; Heloísa Briganti Rodrigues Asenha; Anna Silvia Marques; Antonio Gilberto Ferreira; Edson Rodrigues-Fo. Time course production of indole alkaloids by an endophytic strain ofPenicillium brasilianumcultivated in rice. Natural Product Research 2013, 27, 967 -974.
AMA StyleTaicia Pacheco Fill, Heloísa Briganti Rodrigues Asenha, Anna Silvia Marques, Antonio Gilberto Ferreira, Edson Rodrigues-Fo. Time course production of indole alkaloids by an endophytic strain ofPenicillium brasilianumcultivated in rice. Natural Product Research. 2013; 27 (11):967-974.
Chicago/Turabian StyleTaicia Pacheco Fill; Heloísa Briganti Rodrigues Asenha; Anna Silvia Marques; Antonio Gilberto Ferreira; Edson Rodrigues-Fo. 2013. "Time course production of indole alkaloids by an endophytic strain ofPenicillium brasilianumcultivated in rice." Natural Product Research 27, no. 11: 967-974.