Spodoptera frugiperda Smith (fall armyworm (FAW)) has invaded many countries in Africa and Asia in recent years, considerably restricting global agricultural production. In this study, we assessed the rearing performance of four artificial diets (D₁: an artificial FAW diet based on wheat bran and soybean, maize, and yeast powders; D₂: an artificial diet developed for Helicoverpa armigera (Hübner), based on wheat bran and soybean and yeast powders; D₃: an artificial diet based on soybean powder; D₄: an artificial diet based on wheat bran) for FAWs. We designed D₄ based on a traditional diet (D₂) but substituted the wheat bran for soybean and yeast powders. At 25 ± 1 °C, 75% ± 5% RH, and a 16:8 h L:D photoperiod, the larval stage of FAWs fed on D₄ lasted 15.88 d, the pupal stage lasted 9.48 d, the pupal mass was 270.45 mg, the number of eggs deposited was 1364.78, and the mating rate was 89.53%. Most biological indicators of the larvae that were fed D₄ were basically consistent with those of the larvae fed on the traditional diet (D₂), but the intrinsic rate of increase (r), finite rate of increase (λ), and net reproduction rate (R₀) of the D₄ FAWs were lower than those of the D₂ FAWs. The flight capacity (flight distance, duration, and velocity were 19.73 km, 6.91 h, and 2.90 km/h, respectively) of the D₄ FAWs was comparable to that of the FAWs fed a traditional diet and maize leaves. Compared with the three other formulas, the cost of using D₄ was lower by 26.42% on average. These results show that using cheap wheat bran instead of soybean flour and yeast powder as the basic material for an artificial diet for FAWs is feasible, which will substantially reduce rearing costs and promote the development of new controlling measures for FAWs. In addition, this study also has a reference value for reducing the cost of artificial diets for other insects.

Spodoptera frugiperda, an invasive pest, has a huge impact on food production in Asia and Africa. The potential and advantages of sterile insect techniques for the permanent control of S. frugiperda have been demonstrated, but the methods for their field application are still unavailable. For the purposes of this study, male pupae of S. frugiperda were irradiated with an X-ray dose of 250 Gy to examine the effects of both the release ratio and the age of the irradiated males on the sterility of their offspring. The control effect of the irradiated male release ratio on S. frugiperda was evaluated using field-cage experiments in a cornfield. The results showed that when the ratio of irradiated males to non-irradiated males reached 12:1, the egg-hatching rate of the offspring of S. frugiperda decreased to less than 26%, and there was also no significant difference in mating competitiveness among the different ages. Field-cage testing showed that when irradiated males were released at ratios of 12:1–20:1 to normal males, the leaf protection effect for the corn reached 48–69% and the reduction in the insect population reached 58–83%. In this study, an appropriate release ratio is suggested, and the mating competitiveness of irradiated and non-irradiated males of S. frugiperda is investigated, thus providing a theoretical basis for the use of sterile insect techniques to control S. frugiperda.

As a migratory invasive pest, Spodoptera frugiperda (fall armyworm, FAW) has recently posed a serious threat to food security in newly invaded areas (especially in Africa and Asia). Understanding its migration (or dispersal) patterns in newly invaded areas is crucial for regional forecasting and management efforts. By screening an appropriate marking technique to conduct mark–release–recapture (MRR) experiments, the migration patterns of the FAW can be effectively studied. In this study, we added different concentrations of Calco Oil Red N-1700 (an oil-soluble marker) to a self-made artificial diet and assessed the rearing and marking efficacy. The results indicated that a concentration of 0.2% of Calco Oil Red N-1700 in the diet was optimal for marking adult FAWs. The biological indicators (e.g., developmental duration, reproductive parameters, and flight ability) of FAWs fed this diet were basically consistent with those of FAWs fed a normal diet, with a larval stage of 15.46 days, a pupal stage of 9.81 days, a pupal mass of 278.18 mg, an adult longevity of 15.41 days, and an egg deposition count of 1503.51. Meanwhile, the flight distance, duration, and velocity were 24.91 km, 7.16 h, and 3.40 km/h, respectively (12 h tethered-flight tests), without difference with the control. Females and males exhibited distinctive marking colors (red or pink) that persisted for at least 5 and 9 days, respectively. This study developed an economically effective internal marking method for the adult FAW, laying the foundation for conducting MRR experiments. This will help clarify the migration behavior and routes of the FAW, providing a scientific basis for formulating effective pest management strategies.

The clover cutworm, Anarta trifolii (Lepidoptera: Noctuidae), constitutes a polyphagous pest known for causing sporadic, local outbreaks that significantly damage Beta vulgaris, Gossypium hirsutum, Brassica oleracea and others. Evidence supports the occurrence of seasonal migration in this species, but the determinants of A. trifolii flight performance remain unexplored. Understanding the species’ flight performance is essential for predicting its long-distance dispersal, identifying source and sink populations, and improving regional pest forecasting. We characterized flight performance and its influencing factors via computer-monitored flight mills. Maximum flight performance was achieved in A. trifolii adults at two days, followed by a significant decline with increasing age. At 24 °C and 80% relative humidity (RH), in a 12 h test, males and females aged two days achieved total flight distances of 38.90 ± 1.21 km and 31.70 ± 1.56 km, respectively. In a 24 h test, three-day-old adults reached a maximum flight speed of 19.68 km/h, a sustained flight duration of 17.38 h, a total flight duration of 23.89 h, a sustained flight distance of 69.64 km, and a total flight distance of 96.56 km. The flight performance of A. trifolii was significantly affected by both temperature and RH, with the maximum flight capacity achieved at 18–28 °C and 35–80% RH. Flight performance was significantly enhanced when A. trifolii were fed honey or sucrose. Moreover, the wingbeat frequency of A. trifolii adults varied among age groups, ranging from 31.90 to 57.65 Hz. In females, the wingbeat frequency peaked at 2 days old (46.72 ± 0.25 Hz), whereas in males it peaked at 10 days old (47.18 ± 0.66 Hz). These results advance the fundamental understanding of A. trifolii migration and offer practical applications, including improved pest management strategies, optimized use of chemical insecticides and biological control agents, and enhanced decision-making in integrated pest management programs.