AmelanchierMedik. belonging to the family Rosaceae includes about 30 species with a high content of biologically active substances. The aim of the study was to determine the morpho-anatomical features of leaves at the adaptation and ontogenesis stages of Saskatoon berry variety ‘Krasnoyarskaya’ (A. alnifolia) and variety ‘Prince William’ (A. canadensis). Saskatoon berry leaves are characterized by hypostomatic and anomocytic, less often anisocytic stomata at all stages of ontogenesis. The study revealed in the average length of the polar axis and the stomata equatorial diameter, depending on the cultivation conditions and genotype. A. canadensis had a larger average stomatal area, a thicker epidermis, a greater height of the palisade chlorenchyma cells, and a higher coefficient of palisade, which made it more resistant to changes in atmospheric precipitation, at all stages of ontogenesis, in comparison A. alnifolia. Bangladesh J. Plant Taxon. 30(2): 185-193, 2023 (December)
Cymbidium erythrostylum Rolfe is one of the most beautiful species of the genus cymbidium which is used as a cut flower or indoor plant. However, it was registered as a rare species even in its original habitat. This study was carried out to develop a protocol for in vitro propagation of C. erythrostilum. We used protosomes obtained by the asymbiotic germination of seed on 1/2 of Murashige and Skoog nutrient medium (MS) supplemented with 1 mg/L 2-Isopentenyl adenine (2iP) as objects of study. During the multiplication stage, the number of formed protosomes on a culture medium containing 0.8 mg/L 6-Benzylaminopurine (6-BAP) was higher (3.8 ± 0.2 protosomes). During the subsequent cultivation on ½ MS medium supplemented with 2 mg/L 6-BAP and 0.5 mg/L a-naphthaleneacetic acid (NAA), the highest numbers of shoots (4.00 ± 0.19 shoots/plantlet) and leaves (4.50 ± 0.14 leaves/plantlet) were obtained. At the rooting stage under in vitro conditions, the most effective was the use of ½ MS nutrient medium with the addition of 0.5 mg/L of indolyl-3-butyric acid IBA, 1 g/l charcoal, and 50 g/l banana puree. The obtained plants were successfully adapted to a substrate consisting of bark, perlite, and peat in a ratio of 1:1:1.