Background: Capnophyllum peregrinum (L.) Lange (Apiaceae) is the unique taxon of capnophyllum genus in Algerian flora. It has never been investigated in regards to its total phenolic and flavonoid contents and antioxidant and photoprotective activities. Methods: C. peregrinum aerial parts extracted with absolute methanol. The total flavonoid and phenolic contents of the extract were evaluated to determine their correlation with the antioxidant and photoprotective activities of the extract. Results: The methanolic extract demonstrated a significant amount of phenolics and flavonoids (74.06 ± 1.23 mg GAE/g, 44.09 ± 2.13 mg QE/g, respectively) and exhibited good antioxidant activity in different systems, especially in 1,1-Diphenyl-2-picrylhydrazyl (DPPH), reducing power and total antioxidant capacity assays. Furthermore the extract showed high photoprotective activity with the sun protection factor (SPF) value = 35.21 ± 0.18. Conclusions: The results of the present study show, that the methanolic extract could be used as a natural sunscreen in pharmaceutics or cosmetic formulations and as a valuable source of natural antioxidants.

The current study first describes the extraction of phytoantioxidant polyphenols from Carob byproducts (pods) using maceration and heating-assisted extraction as traditional methods and ultrasonic-assisted extraction (UAE) as an innovative method to determine the most efficient extraction process in terms of four targeted responses: total phenolic content (TPC), antioxidant activities (TAC and DPPH), and photoprotective properties as measured by the sun protection factor (SPF). Second, we used response surface methodology (RSM) with a central composite rotatable design (CCDR) approach to investigate the influence of process variables (extraction time, extraction temperature, and solvent concentration) on UAE, which was found to be the most effective extraction technique in our study. Carob byproduct extracts had a TPC ranging from 6.21 to 21.92 mg GAE/g dw, a TAC ranging from 22.00 to 49.30 mg AAE/g dw, DPPH scavenging activity ranging from 56.35 to 90.50%, and SPF values ranging from 8.62 to 22.37. The optimal UAE conditions for maximum TPC, TAC, DPPH, and SPF responses were determined to be 38.90% ethanol, 53.90 °C, and 50.92 min. Using Carob as a source for sustainable and bioactive products in conjunction with optimized UAE is a promising contribution to the cosmetic industry that will help to strengthen the concept of environmentally-friendly “green chemistry”. Given that Carob pulp or seeds are considered food byproducts, the research presented here encourages the use of these agri-food waste materials in cosmetics.

The purpose of this work is to develop two types of dietary supplements for celiac (energy-rich and fiber-rich bars) as well as to optimize the formulations of bars made from puffed and non-puffed cereals. To optimize the combination of components, a mixture design was created. Based on sensory evaluation, optimal bars were selected, which were then evaluated in terms of biochemical properties, color and antioxidant properties. The main results indicate that the combination of 37.5 g of cereals, 22.5 g of seeds, and 40 g of binder is optimal for the energy bars with non-puffed cereals, followed by 54.57 g of cereals, 10.43 g of seeds, and 35 g of a binder for fiber-rich bars with non-puffed cereals. In contrast, the optimal recipe for energy bars with puffed cereals consisted of 35.42 g of cereals, 20.07 g of seeds, and 44.51 g of binder, and for fiber-rich bars with puffed cereals, it consisted of 50 g of cereals, 15 g of seeds, and 35 g. The biochemical composition indicates that fiber-rich bars are also energetic, with more than 300 kcal/100 g. All bars are rich in antioxidants, with total polyphenol values exceeding 4.97 mg GAE/g d.w. Customers prefer the bars with puffed cereal the most.

Purpose: To assess the polyphenolic composition of various solvent extracts of Jurinea humilis DC. as well as their in vitro biological effects. Methods: The crude extracts of the aerial parts of Jurinea humilis were obtained by maceration method with dichloromethane (DCM), methanol (MeOH) and ethyl acetate (EtoAC), separately. Folin-Ciocalteu and aluminum chloride procedures were used to quantify the total phenolic and flavonoid contents, respectively, while antioxidant properties were determined by two methods: phosphomolybdenum and DPPH assays. Antimicrobial activity was also evaluated by disc diffusion method. Results: The ethyl acetate extract exhibited the highest amount of total phenolic content (TPC, 169.14 ± 7.22 mg gallic acid equivalent/g sample) and total flavoniod contents (TFC, 104.91±0.22 mg rutin equivalent/g sample). The extract (EtoAC) also showed the strongest antioxidant activity of the three extracts, especially DPPH assay (IC₅₀ = 0.16 ± 0.00 mg/mL), as well as demonstrated the highest activity against the pathogenic strains tested. Conclusion: The results suggest that J. humilis DC. is rich in phenolic and flavonoid contents which may be responsible for good antioxidant and antimicrobial properties. Thus, J. humilis may be a promising source of new antioxidant agents and pharmaceuticals. Keywords: Jurinea humilis, Phenolic, Flavonoid, Antioxidant, Antimicrobial

Graphical abstract Abstract During the current study, the phenolic and flavonoid contents were measured in Physalis acutifolia extracts with Folin–Ciocalteu and AlCl₃ methods, respectively. Various antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl, ABTS, reducing power, Fe²⁺-phenanthroline reduction, and silver nanoparticle assays, were also conducted, along with anti-enzymatic assays. The cytotoxicity of the ethanolic extract was assessed on cancer cell lines (i.e., CAPAN-1 and dld-1) and a healthy cell line (i.e., L929). Optimal parameters for polyphenol extraction were determined: extraction time of 72 h, solvent-to-plant ratio of 10 mL/1 g, and plant concentration of 90%. The n-butanol extract showed the highest phenolic and flavonoid contents (i.e., 263.84 mg GAE/g dw and 72.03 QE/g dw, respectively), along with superior antioxidant and anti-enzymatic activities with IC₅₀ values of 49.77 and 187.12 µg/mL with acetylcholinesterase and butyrylcholinesterase assays, respectively. LC-ESI-MS/MS analysis revealed 12 components, with hesperidin being the most abundant (i.e., 1829.0001 µg/g). The ethanolic extract exhibited cytotoxic effects on cancer cell lines, with an IC₅₀ value of 0.959 mg/mL for dld-1, but with no effect on healthy cells. The bioavailability scores of the identified components support the in vitro findings and confirm the different assessed therapeutic effects.