Artificial reefs (ARs) are one of the key anthropogenic constructs used to restore offshore fishery resources and recover the ecological environment. However, many ARs lose their stability and function due to scour. To ensure the functional effect of ARs, it is of great significance to study the factors causing AR instability, such as burying caused by scour under different flow conditions. In present study, a three-dimensional numerical model is established in FLOW-3D to study the local scour characteristics around an AR in steady currents. Reynolds-averaged Navier–Stokes (RANS) equations, closed with the renormalization group (RNG) k–ɛ turbulence model, are established to simulate a stable flow field around one AR. The simulation results are compared with previous experimental results, exhibiting good agreement. The effects of the opening number and the incident angles of ARs on the scour characteristics, equilibrium scour depth, and maximum scour volume were also investigated. The results indicate that the scour depth and scour volume decreased as the opening number increased. Furthermore, empirical equations are proposed herein based on the numerical results derived for the effects of the AR opening number on the equilibrium scour depth and maximum scour volume. A change in the incident angle affected the bed shear stress at the most-upstream corner of the AR. The greater the bed shear stress was, the more intense the scour was. In this study, we provide theoretical support and practical guidance for the optimized engineering design and construction of ARs.

Beta diversity is an important way to analyze community assembly mechanisms in different habitats or along environmental gradients. However, research on marine fish assemblages around islands has lagged, especially for functional beta diversity. In this study, we evaluated taxonomic and functional beta diversity change of island fish assemblages along the coast in two seasons and revealed its relationship with environmental factors and geographical distance. Taxonomic and functional beta diversity were both dominated by turnover (over 80% and 60%), while the contribution of nestedness on functional beta diversity was significantly increased. Environmental factors such as temperature and dissolved oxygen were important drivers of beta diversity rather than geographical distance. Fish assemblages around islands that are far away from mainlands or affected greatly by anthropogenic activities usually have higher beta diversity. These results indicated that environmental filtration is the primary factor driving the mechanism of fish community assembly. Our study revealed the importance of the integrated application of two facets of biodiversity to investigate beta diversity. The findings can provide theoretical support for the protection of marine fish and the planning of marine protected areas in the future.

Pacific oysters (Crassostrea gigas) are widely cultured in Chinese marine ranching with high economic value. However, mass death of farmed oysters has occurred frequently in recent years because of diseases and environmental disturbance (e.g., high temperatures). In order to analyze the potential relationships between microorganisms and the death of farmed oysters, we compared the dynamics of bacterial and protist communities in oysters at different growth phases using high-throughput sequencing. The results showed that the microbial communities in farmed oysters significantly changed and were markedly different from microbes in natural oysters and the surrounding environments. The number of biomarker taxa among farmed oysters and their surrounding environments decreased gradually with the growth of oysters. During the mass death of farmed oysters, the microbial communities’ abundance of ecological function genes changed, and the correlations among microorganisms disappeared. These results enrich our understanding of the dynamics of microbial communities in farmed oysters at different growth phases, illustrating the characteristics of interactions among microorganisms during the mass death of farmed oysters. Our study is beneficial to promote the healthy aquaculture of oysters.

Due to overfishing and marine pollution, the status of fishery resources and the ecological environment in the sea areas have considerably changed. The proliferation and release of fishery resources have become the main means of human intervention to maintain and conserve the resource populations. The annual output of Sepiella japonica (S. japonica), once one of the four major seafood species in the East China Sea, has now recovered to a level of 4000 t. However, do the traditional spawning grounds of S. japonica still exist? Have the spawning grounds shifted? These scientific questions are worthy of attention. Based on the survey data of fishery resources and environment at 120 stations in coastal Zhejiang waters from 2015 to 2022, the spatial and temporal distribution and resource center of gravity of S. japonica in springtime were analyzed. Random forest (RF) was used to explain the importance of six environmental variables, including water depth, sea surface temperature, sea surface salinity, chlorophyll a, pH, and dissolved oxygen. The generalized additive model (GAM) nonparametric smoothing function was used to analyze the relationship between environmental factors and the distribution of S. japonica inhabiting the offshore areas of Zhejiang province, and the effects of environmental factors on spawning habitat selection of S. japonica were revealed. This study found that there was a significant interannual variation in S. japonica resources, with an overall increasing trend in the resource. The spawning grounds were mainly distributed in the Jiushan Islands Marine Reserve, the Dachen Islands Marine Reserve, and their nearby sea areas. The resource peaked at latitude 28.3° N. Additionally, the most important variables affecting the distribution of S. japonica were depth of water, followed by sea surface salinity, pH, dissolved oxygen, sea surface temperature, and chlorophyll a. S. japonica mainly inhabited sea areas with a depth of 15~25 m and a sea surface salinity of 26~32. When the pH ranged from 7.6 to 8.3, dissolved oxygen ranged from 6 to 9 mg/L, sea surface temperature ranged from 14 to 17 °C, and chlorophyll a ranged from 2.5 to 5 µg/L, S. japonica was more likely to be present. This study provides insights into the spatial distribution of S. japonica in offshore Zhejiang province, offering a reference for the rational utilization and scientific protection of this resource.

The coastal waters of Zhejiang feature a complex aquatic environment and abundant biological resources, creating an ideal habitat for various fish species. However, the systematic monitoring of fish larvae in these offshore waters is limited. This study collected 24,232 fish larvae using large plankton nets during April and November 2022, as well as February and July 2023, and identified 93 species, primarily warm-temperate and warm-water species, with a peak occurrence in summer. The dominant species include Larimichthys croceus, Sebastiscus marmoratus, Lateolabrax japonicus, and Odontamblyopus lacepedii, among others, and these species exhibit frequent seasonal changes. Fish larvae are typically found to be aggregated along estuaries and bays in spring, autumn, and summer, while in winter, they tend to shift towards areas near the boundaries of motor trawler fisheries areas. Our cluster analysis revealed spatial heterogeneity in the community structure, driven by an abundance of dominant and important species. Our Mantel tests and canonical correspondence analysis (CCA) identified seawater temperature and salinity as core drivers of the aggregation and distribution of fish larvae, interacting with factors such as the chlorophyll-a concentration, water turbidity, water depth, and dissolved oxygen. This research provides a scientific basis for the dynamic monitoring of spawning grounds and effective management of fishery resources in Zhejiang’s coastal waters.

Biodiversity is the cornerstone of marine fisheries. To ensure the prosperity of stow net fishery in Haizhou Bay, regular investigations of fishery resources are essential. However, most studies have primarily focused on taxonomic diversity while overlooking functional diversity. In this study, we examined both the taxonomic and functional diversity of fish assemblages based on abundance and functional traits from 2013 to 2018. Significant differences in taxonomic diversity were observed only between two seasons, whereas functional diversity showed significant differences across years, seasons and groups. Diversity indices exhibited negative linear relationships with catch per unit effort, except for functional evenness (FEve) and functional divergence. Twenty-seven out of 30 correlations between the two types of diversity indices were predominantly positive and synclastic, whether linear or nonlinear. Functional dispersion showed the most positive relationships with taxonomic diversities, while FEve exhibited gentle slopes. The functional redundancy curves indicated that the ecological stability and resilience of fish assemblages were vulnerable. The non-target fish group demonstrated a higher overlap in functions compared to the target fish group and the total fish group. In summary, the taxonomic and functional diversities revealed inconsistent statuses and trends of fish assemblages, with an evident decreasing trend in the non-target fish group requiring special attention. This study highlights that both taxonomic and functional diversity should be considered simultaneously in fish biodiversity investigations, which is crucial for establishing effective fish conservation systems.