Due to drought stress, durum wheat production in the Mediterranean basin will be severely affected in the coming years. Durum wheat cultivation relies on a few genetically uniform "modern" varieties, more productive but less tolerant to stresses, and "traditional" varieties, still representing a source of genetic biodiversity for drought tolerance. Root architecture plasticity is crucial for plant adaptation to drought stress and the relationship linking root structures to drought is complex and still largely under-explored. In this study, we examined the effect of drought stress on the roots’ characteristics of the “traditional” Saragolla cultivar and the “modern” Svevo. By means of “SmartRoot” software, we demonstrated that drought stress affected primary and lateral roots as well as root hair at different extents in Saragolla and Svevo cultivars. Indeed, we observed that under drought stress Saragolla possibly revamped its root architecture, by significantly increasing the length of lateral roots, and the length/density of root hairs compared to the Svevo cultivar. Scanning Electron Microscopy analysis of root anatomical traits demonstrated that under drought stress a greater stele area and an increase of the xylem lumen size vessel occurred in Saragolla, indicating that the Saragolla variety had a more efficient adaptive response to osmotic stress than the Svevo. Furthermore, for the analysis of root structural data, Artificial Intelligence (AI) algorithms have been used: Their application allowed to predict from root structural traits modified by the osmotic stress the type of cultivar observed and to infer the relationship stress-cultivar type, thus demonstrating that root structural traits are clear and incontrovertible indicators of the higher tolerance to osmotic stress of the Saragolla cultivar. Finally, to obtain an integrated view of root morphogenesis, phytohormone levels were investigated. According to the phenotypic effects, under drought stress,a larger increase in IAA and ABA levels, as well as a more pronounced reduction in GA levels occurred in Saragolla as compared to Svevo. In conclusion, these results show that the root growth and hormonal profile of Saragolla are less affected by osmotic stress than those of Svevo, demonstrating the great potential of ancient varieties as reservoirs of genetic variability for improving crop responses to environmental stresses.

Durum wheat is widely cultivated in the Mediterranean, where it is the basis for the production of high added-value food derivatives such as pasta. In the next few years, the detrimental effects of global climate change will represent a serious challenge to crop yields. For durum wheat, the threat of climate change is worsened by the fact that cultivation relies on a few genetically uniform, elite varieties, better suited to intensive cultivation than “traditional” ones but less resistant to environmental stress. Hence, the renewed interest in “ancient” traditional varieties are expected to be more tolerant to environmental stress as a source of genetic resources to be exploited for the selection of useful agronomic traits such as drought tolerance. The aim of this study was to perform a comparative analysis of the effect and response of roots from the seedlings of two durum wheat cultivars: Svevo, a widely cultivated elite variety, and Saragolla, a traditional variety appreciated for its organoleptic characteristics, to Polyethylene glycol-simulated drought stress. The effect of water stress on root growth was analyzed and related to biochemical data such as hydrogen peroxide production, electrolyte leakage, membrane lipid peroxidation, proline synthesis, as well as to molecular data such as qRT-PCR analysis of drought responsive genes and proteomic analysis of changes in the protein repertoire of roots from the two cultivars.

Drought stress is increasing in frequency and severity with the progression of global climate change, thereby becoming a major concern for the growth and yield of crop plants, including wheat. The current challenge is to explore different ways of developing wheat genotypes with increased tolerance to drought. Therefore, we renewed interest in ‘ancient’ varieties expected to be more tolerant to environmental stress than the few elite varieties nowadays cultivated. This study aimed to perform comparative analysis of the effect of drought-simulating polyethylene glycol (PEG-6000) treatment on morpho-anatomical and physiological foliar traits of two durum wheat seedlings cultivars, Saragolla and Svevo, as these can reflect the adaptability of the plant to the environment to a certain extent. Results demonstrated that drought-stressed Saragolla leaves exhibited a greater reduction of stomatal density, a minor reduction of stomatal pore width, a wider xylem vessel mean area, greater compactness of mesophyll cells, a minor loss of chlorophyll content, as well as better photosynthetic and growth performance compared to the other variety. From such behaviours, we consider the Saragolla cultivar more drought tolerant than Svevo and therefore probably very promising for cultivation in dry areas.

Climate change is leading to an increase in the intensity, duration, and frequency of severe droughts, especially in southern and southeastern Europe, thus aggravating water scarcity problems. Water deficit stress harms the growth, physiology, and yield of crops like durum wheat. Hence, studying ancient wheat varieties’ stress responses could help identify genetic traits to enhance crop tolerance to environmental stresses. In this background, this study aimed to investigate the effects of PEG 6000-stimulated drought stress in the ancient wheat variety Saragolla and the modern one Svevo by analyzing various biochemical and molecular parameters that can especially condition the stomatal movement. Our data revealed that drought stress caused a significant increase in the levels of total soluble sugars, ABA, and IAA in both selected cultivars to a greater extent in the Saragolla than in the Svevo. We demonstrated that, under water deficit stress, calcium dynamics as well as the expression of ERF109, MAPK3/6, MYB60, and TaTPC1, involved in the activation of drought-related calcium-sensitive pathways, display significant differences between the two varieties. Therefore, our study provided further evidence regarding the ability of the ancient wheat variety Saragolla to better cope with drought stress compared to the modern variety Svevo.

Recent studies have highlighted the potential of plant-based and agro-industrial by-products as valuable sources of bioactive compounds for animal feed formulation. This study aims to evaluate the impact of dietary supplementation with Olea europaea L. leaf extract on the fatty acid composition of pig muscle. Thirty commercial hybrid pigs (Large White × (Landrace × Duroc)), with an initial body weight of 169 ± 7.90 kg and an average age of 10 ± 1 months, were randomly assigned to two experimental groups (n = 15 per group): one fed a standard diet containing Sulla (C) and the other receiving the same diet enriched with olive leaf extract (OL). Over 90 days, the OL group was supplemented with 300 mg/head/day of olive leaf extract, primarily containing oleuropein diglucoside, luteolin-7-glucoside, and verbascoside. The phenolic content, antioxidant activity, bioactive compounds, and fatty acid profiles of both meats and diets were analyzed. Results showed that OL supplementation did not significantly affect the chemical composition of the meat or pig performance, but it did influence the lipid profile. Specifically, OL supplementation led to a significant reduction in saturated fatty acids (SFAs) and an increase in oleic acid, thus enhancing the proportion of monounsaturated fatty acids (MUFAs). Moreover, the n-6/n-3 PUFA ratio in the meat was significantly reduced, suggesting potential improvements in the nutritional and functional quality of pig meat, enhancing the nutritional and functional quality of the meat by improving its fatty acid profile.

The escalating global population has heightened the demand for food and feed, making the effects of abiotic stresses on crop yield and quality a significant challenge for global food security. Environmental stressors, such as drought, salinity, heat, disturb plant metabolic functions, redirecting energy from growth processes toward stress adaptation, which ultimately reduces plant productivity. The development of crop varieties resilient to these abiotic stresses is imperative to address the urgent need for sustainable food production. Recent advancements highlight the potential of crops with improved root systems, supported by phytohormone regulation, to enhance water and nutrient uptake, sequester more carbon, and demonstrate greater resilience to climate change. In turn, these increase the potential for crops to stabilize yields under fluctuating environmental conditions. This chapter examines the physiological, molecular, and genetic mechanisms that govern root system architecture (RSA), focusing on the regulatory pathways that influence root growth, branching, and overall adaptability to stress. Furthermore, the chapter discusses the application of advanced phenotyping that has revolutionized our ability to study root traits linked to stress resilience. The integration of this technology with molecular genetics and breeding strategies is accelerating the development of climate-resilient crops.