Bee pollens are potential functional food ingredients as they contain essential nutrients and a wide range of bioactive compounds. The aim of this study was to investigate the effects of enrichment with monofloral bee pollens on the nutritional properties, techno-functional parameters, sensory profile, and consumer preference of biscuits. Biscuits were prepared according to the AACC-approved method by substituting wheat flour with pollens of rapeseed (Brassica napus L.), phacelia (Phacelia tanacetifolia Benth.) and sunflower (Helianthus annuus L.) at 2%, 5% and 10% levels. The macronutrient composition of the biscuits was determined: crude protein content (Kjeldahl method), crude fat content (Soxhlet extraction), ash content (carbonization), moisture content (drying), carbohydrate content (formula). Their total phenolic content (TPC) and in vitro antioxidant capacity (FRAP, TEAC, DPPH) were determined spectrophotometrically. The colour of the biscuits was measured using a tristimulus-based instrument, and their texture was characterized by using a texture analyser. Sensory profile of biscuits was determined by qualitative descriptive analysis (QDA). The consumer acceptance and purchase intention of the biscuits were also evaluated, based on the responses of 100 consumers. Additionally, an external preference map was created to illustrate the relationship between consumer preference and the sensory profile of the biscuits, and penalty analysis was conducted to identify directions for product development. Phacelia pollen appeared to be the most effective for improving the nutritional quality of biscuits. The addition of phacelia pollen at the 10% substitution level increased the protein content and TPC of the control biscuit by 21% and 145%, respectively. Significant changes (p < 0.05) were also observed regarding the colour and texture of biscuits. The results of the QDA revealed that biscuits containing pollens of different botanical sources have heterogeneous sensory attributes. The biscuit containing sunflower pollen at the 2% substitution level was preferred the most (overall liking = 6.9 ± 1.6), and purchase intentions were also the highest for this product. Based on the results of the present study, it is recommended to use sunflower pollen for developing pollen-enriched foods in the future.

Scientific evidence suggests that apicultural products accumulate pollutants present in the hive environment, thus, they can be used as bioindicators. However, our understanding on the food safety implications of the presence of potentially toxic trace elements in these products remains incomplete. In our study, available data on the trace metal content of bee bread, propolis, beeswax and royal jelly, as well as their possible sources are reviewed. Furthermore, dietary risk assessments were conducted for elements that do not have any biological role in humans by comparing the estimated exposures with official reference values. In the case of elements with genotoxic carcinogen potential, the margin of exposure (MoE) approach was applied. The observed concentration ranges vary over a wide range for Fe (0.94-2125.20 mg/kg), Zn (<LOQ - 2790.00 mg/kg), Cu (<LOQ - 40.93 mg/kg), Mn (<LOQ - 204.80 mg/kg), Ni (<LOQ - 75.90 mg/kg), Cr (<LOQ - 56.28 mg/kg), Pb (<LOQ - 160.10 mg/kg), As (<LOQ - 8.47 mg/kg), Cd (<LOQ - 76.69 mg/kg) and Hg (<LOQ - 1.7 mg/kg) in beehive products from different geographical origins. These variances can be attributed to the diversity of soil types, climatic conditions, floral sources, beekeeping practices and anthropogenic activities in the environment. Available data suggest that Pb can be present in apicultural products at concentrations exceeding a thousand μg/kg, which poses a significant food safety threat to long-term consumers.

In our study, Central and Eastern European bee pollens of different botanical origins were compared, based on their antioxidant and color properties. Total phenolic content (TPC), total flavonoid content (TFC), and in vitro antioxidant capacity (by FRAP, CUPRAC, ABTS⋅⁺ and DPPH⋅ assays) were determined spectrophotometrically. Besides, Relative Antioxidant Capacity Indexes (RACI) were calculated. CIELAB color parameters (L*, a*, b*, chroma) were determined by using a tristimulus‐based instrument. Potential correlations between the investigated parameters were also identified. Based on the results of the preliminary study, ethanol:distilled water (60 : 40) was chosen as an extraction solvent. The total phenolic content of our samples ranged between 9.41 and 27.49 mg GAE/g dw. Pollens showed TFC:TPC ratios between 9 and 44 %. RACI values indicate that rapeseed (Brassica napus), traveller's joy (Clematis vitalba) and phacelia (Phacelia tanacetifolia) pollens have relatively high, while pollens of certain plants of the Asteraceae family possess low antioxidant potential. Antioxidant properties correlated significantly in most cases. RACI values showed strong positive correlation with each of the other antioxidant capacity parameters, suggesting that this approach is well applicable for comparing the antioxidant potential of bee pollens. No clear correlation was found between the antioxidant and color parameters.

The volatile profile of bee pollen samples from Central and Eastern Europe was investigated by headspace solid phase microextraction (HS‐SPME) combined with gas chromatography–mass spectrometry‐olfactometry (GC–MS‐O). Sampling conditions were optimized for the extraction of volatiles. Pollen odorants were extracted with six different fiber coatings, five various extraction times, three diverse extraction temperatures and three differing desorption times. The most effective combination was the application of divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber coating used at 60°C for 30 min for extraction and 1 min for desorption. The optimized method was applied to investigate the volatile profile of 14 pollen samples (three rapeseed, musk thistle, rock‐rose, traveler's joy, dropwort, honey locust, sunflower, red poppy, phacelia, sweet cherry, wild blackberry, and dandelion). The volatile profiles of bee pollens were different and were crucially depended on botanical origin. The aroma activity of the samples was generated by 31.0%–48.3% of total volatiles. The number of the identified odorants were between 75 and 101 in the pollen samples by GC–MS, of which 26–42 were aroma‐active. The volatile organic compounds (VOCs) were classified into 13 different chemical classes. In most pollen, fatty acids were the predominant volatiles (14.87%–50.58%), while in some samples esters were the most abundant odorants (4.09%–45.46%). Panelists confirmed the presence of six main sensory characteristics described as “green/sour”, “fruity”, “spicy/herbal”, “earthy/mushroom”, “sweet/baked/caramel/honey”, and “floral” compounds. These results establish the flavor wheel suitable for the comprehensive sensory description of pollen pellets from individual plant species. All samples contained characteristic odorants that may help in their botanical identification.

In recent decades, there has been an increasing consumer demand for the so-called superfoods, accompanied by a growing scientific interest. In our work, sixteen commercial nutraceuticals of different product categories, including powders of cereal grasses, vegetables, fruits, microalgae, an aquatic plant, and herbs, were analyzed to compare their nutritional properties and volatile profile. Some samples, especially spirulina, chlorella, and duckweed powders, were rich in protein; however, their amino acid composition was imbalanced. Spinach, duckweed, and moringa powders exhibited very high ash contents, while sea buckthorn powder was a relatively rich source of lipids. Acerola powder had the highest total phenolic content by far and showed exceptional antioxidant and antibacterial properties in in vitro analyses as well. Aronia and rosehip powders also had high antioxidant potential, while moringa powder exhibited remarkable antibacterial activity. The headspace volatiles of the samples showed significant variations. Terpenes were the major volatiles in herbs, especially in turmeric and ginger powders. High levels of acids were detected in fruit powders. Some compounds were only characteristic of certain products, for example, dimethyl sulfide in beetroot powder, trans-3-penten-2-one in aronia powder, hexadecane in spirulina powder, and turmerones in turmeric powder. These volatiles can be considered botanical markers, which potentially help detect the adulteration of specific nutraceuticals. Overall, this study presents the nutritional and aromatic diversity among various “superfood” powders, highlights their potential application as ingredients in functional foods, and emphasizes the need for regulatory standards to ensure product safety, quality, and consistency in the growing nutraceutical market.