One of the new methods of protecting and supporting plant growth is the use of low-temperature plasma. The aim of this study is to evaluate the feasibility of using plasma activated water produced in an atmospheric pressure gliding arc reactor for germination of beetroot (Beta vulgaris) and carrot (Daucus carota) seeds. The study was carried out for different plasma treatment times of water (5, 10 and 20 min) and with fixed geometry and power of the discharge system, using air as the working gas. The effect on germination was evaluated based on the fraction of germinated seeds and their length at 7 and 14 days after treatment. Analysis of fungi present on the seed surface and imaging of the seed surface using scanning electron microscopy (SEM) were auxiliary methods to evaluate the type of treatment effect. In the case of beetroot, a positive effect on the number and length of germinated seeds was observed, which increased with increasing treatment time. This effect can be attributed, among other things, to the surface changes observed on microscopic photographs. In the case of carrot seeds, a more significant positive effect on germination was observed. Fungal decontamination effect was relatively weaker than with the use of the chemical method with sodium hypochlorite.

Low-temperature atmospheric pressure plasma was demonstrated to have an ability to generate different reactive oxygen and nitrogen species (RONS), showing wide biological actions. Within this study, mesoporous silica nanoparticles (NPs) and Fe_xO_y/NPs catalysts were produced and embedded in the polysaccharide matrix of chitosan/curdlan/hydroxyapatite biomaterial. Then, basic physicochemical and structural characterization of the NPs and biomaterials was performed. The primary aim of this work was to evaluate the impact of the combined action of cold nitrogen plasma and the materials produced on proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (ADSCs), which were seeded onto the bone scaffolds containing NPs or Fe_xO_y/NPs catalysts. Incorporation of catalysts into the structure of the biomaterial was expected to enhance the formation of plasma-induced RONS, thereby improving stem cell behavior. The results obtained clearly demonstrated that short-time (16s) exposure of ADSCs to nitrogen plasma accelerated proliferation of cells grown on the biomaterial containing Fe_xO_y/NPs catalysts and increased osteocalcin production by the cells cultured on the scaffold containing pure NPs. Plasma activation of Fe_xO_y/NPs-loaded biomaterial resulted in the formation of appropriate amounts of oxygen-based reactive species that had positive impact on stem cell proliferation and at the same time did not negatively affect their osteogenic differentiation. Therefore, plasma-activated Fe_xO_y/NPs-loaded biomaterial is characterized by improved biocompatibility and has great clinical potential to be used in regenerative medicine applications to improve bone healing process.

This paper presents an experiment using a radio frequency atmospheric pressure plasma jet to generate cold plasma for pre-sowing stimulation of Wolska onion seeds. Impact of the He + N₂ afterglow plasma on germination was investigated. Eight groups of seeds characterized by different exposure times (2, 5, 10, 15, 60, 120, 240, and 480 s) and distance from the electrodes (20 mm and 50 mm) were used. Pre-sowing plasma stimulation of the seeds improved the germination capacity and germination energy for all tested groups, relative to control. The impact of radio frequency plasma on the onion seed germination parameters was statistically significant. The highest germination parameters were obtained for seeds stimulated for 240 s at a distance of 50 mm. No significant differences in physical and morphological properties of onion seeds were found.

This paper presents the results of an experiment based on using a Glide-arc type plasma reactor operating at atmospheric pressure for the quality of fresh pressed tomato juice, variety Bekas. The impact of after-glow plasma gas (air) on the physicochemical, microbiological properties and morphology of the product’s samples was investigated. Five groups of juices characterized by different exposure times (30, 60, 120, 300 and 600 s), as well as untreated juice (as control) were used. The juice quality was assessed on days 1, 3, 5, and 10 of refrigerated storage. Significant increases were observed when Cold Atmospheric Plasma (CAP)-treated tomato juice was tested against total soluble solids, pH, lycopene, and vitamin C in comparison to the control treatments. Moreover, changes in the tested physicochemical values during the storage of juice subjected to the action of cold plasma did not progress as quickly as in the case of the control juice. A significant decrease was observed in total plate count, yeast, and mold after 300–600 s CAP treatment. The findings of the current study suggested that CAP treatment is a promising technique that could provide improved quality and stability during the processing of tomato juice with better physicochemical properties and bioavailable nutrients.

In the technological processes requiring mild treatment, such as soft materials processing or medical applications, an important role is played by non-equilibrium plasma reactors with dielectric barrier discharge (DBD), that when generated in noble gases allows for the effective treatment of biological material at a low temperature. The aim of this study is to determine the operating parameters of an atmospheric pressure, radio-frequency DBD plasma jet reactor for the precise treatment of biological materials. The tested parameters were the shape of the discharge (its length and volume), current and voltage signals, as well as the power consumed by the reactor for various composition and flow rates of the working gas. To determine the applicability in medicine, the temperature, pH, concentrations of H₂O₂, NO₂⁻ and NO₃⁻ and Escherichia coli log reduction in the plasma treated liquids were determined. The obtained results show that for certain operating parameters, a narrow shape of plasma stream can generate significant amounts of H₂O₂, allowing for the mild decontamination of bacteria at a relatively low power of the system, safe for the treatment of biological materials.

Sterilization of municipal waste for a raw material for the production of refuse-derived fuel and to protect surface and ground waters against biological contamination during transfer and storage creates a lot of problems. This paper evaluates the antimicrobial potential of non-equilibrium plasma in relation to the selected groups of microorganisms found in humid waste. The proposed research is to determine whether mixed municipal waste used for the production of alternative fuels can be sterilized effectively using low-temperature plasma generated in a gliding arc discharge reactor in order to prevent water contamination and health risk for working staff. This work assesses whether plasma treatment of raw materials in several process variants effectively eliminates or reduces the number of selected groups of microorganisms living in mixed municipal waste. The presence of vegetative bacteria and endospores, mold fungi, actinobacteria Escherichia coli, and facultative pathogens, i.e., Staphylococcus spp., Salmonella spp., Shigella spp., Enterococcus faecalis and Clostridium perfringens in the tested material was microbiologically analyzed. It was found that the plasma treatment differently contributes to the elimination of various kinds of microorganisms in the analyzed raw materials. The effectiveness of sterilization depended mainly on the time of raw materials contact with low-temperature plasma. The results are very promising and require further research to optimize the proposed hygienization process.