The real-time nondestructive monitoring of plant water content can enable operators to understand the water demands of crops in a timely manner and provide a reliable basis for precise irrigation. In this study, a method for rapid estimation of water content in Aquilaria sinensis using multispectral imaging was proposed. First, image registration and segmentation were performed using the Fourier–Mellin transform (FFT) and the fuzzy local information c-means clustering algorithm (FLICM). Second, the spectral features (SFs), texture features (TFs), and comprehensive features (CFs) of the image were extracted. Third, using the eigenvectors of the SFs, TFs, and CFs as input, a random forest regression model for estimating the water content of A. sinensis was constructed, respectively. Finally, the monarch butterfly optimization (MBO), Harris hawks optimization (HHO), and sparrow search algorithm (SSA) were used to optimize all models to determine the best estimation model. The results showed that: (1) 60%–80% soil water content is the most suitable for A. sinensis growth. Compared with waterlogging, drought inhibited A. sinensis growth more significantly. (2) FMT + FLICM could achieve rapid segmentation of discrete A. sinensis multispectral images on the basis of guaranteed accuracy. (3) The prediction effect of TFs was basically the same as that of SFs, and the prediction effect of CFs was higher than that of SFs and TFs, but this difference would decrease with the optimization of the RFR model. (4) Among all models, SSA-RFR_CFs had the highest accuracy, with an R² of 0.8282. These results confirmed the feasibility and accuracy of applying multispectral imaging technology to estimate the water content of A. sinensis and provide a reference for the protection and cultivation of endangered precious tree species.

Shade management, which is widely adopted in cultivation and understory regeneration, alters plant light environments, thereby degrading the trait inversion performance and posing a key challenge in plant phenotyping. To address this issue, this study reframed chlorophyll retrieval of Hopea hainanensis under shade management as an illumination-regime-dependent conditional domain shift problem, and developed a condition-aware domain adaptation framework (CAI-DAI) tailored to this setting. The results showed that chlorophyll content increased with shading intensity, accompanied by clear differences in canopy spectral distributions among shading levels, supporting the presence of condition-dependent variation under shade management. Model comparisons showed that CA-IE and CAI-DAI, which integrate conditional encoding and conditional alignment, performed better than the comparative models across fine-tuning ratios from 30% to 70%. Among them, CAI-DAI achieved the best and most stable performance, with test MAE ranging from 4.355 to 4.774 μg·cm⁻² and nRMSE ranging from 16.4% to 18.2%, and R² ranging from 0.456 to 0.585. Further evaluation at individual shading levels (S1–S4) showed that CAI-DAI produced narrower error ranges than CA-IE. It also showed smaller error fluctuations under most fine-tuning ratios. These results demonstrate that the proposed framework effectively improves robustness under heterogeneous shading conditions and limited labeled samples, providing methodological support for chlorophyll monitoring and decision-making related to shade management.