Prodigiosin pigment has high medicinal value, so exploring this compound is a top priority. This report presents a prodigiosin bioactive compound isolated from Serratia marcescens JSSCPM1, a new strain. The purification process of this compound involves the application of different chromatographic methods, including UV-visible spectroscopy, high-performance liquid chromatography (HPLC), and liquid chromatography–mass spectrometry (LC/MS). Subsequent analysis was performed using nuclear magnetic resonance (NMR) to achieve a deeper understanding of the compound’s structure. Finally, through a comprehensive review of the existing literature, the structural composition of the isolated bioactive compound was found to correspond to that of the well-known compound prodigiosin. The isolated prodigiosin compound was screened for antibacterial activity against both Gram-positive and Gram-negative bacteria. The compound inhibited the growth of Gram-negative bacterial strains compared with Gram-positive bacterial strains. It showed a maximum minimum inhibitory concentration against Escherichia coli NCIM 2065 at a 15.9 ± 0.31 μg/mL concentration. The potential binding capabilities between prodigiosin and the OmpF porin proteins (4GCS, 4GCP, and 4GCQ) were determined using in silico studies, which are generally the primary targets of different antibiotics. Comparative molecular docking analysis indicated that prodigiosin exhibits a good binding affinity toward these selected drug targets.

Background: Diabetes mellitus (DM) is a prevalent chronic metabolic condition characterized by high blood sugar levels, resulting from insufficient insulin production or ineffective insulin use, posing substantial global health issues. Research on the relationship between glycemic status and the ratio of neutrophils to lymphocytes (NLR) and monocytes to lymphocytes (MLR) is limited. This study aimed to fill these knowledge gaps by examining the connection between DM and inflammatory markers within the Asir region. Methods: Data from 3545 participants were retrospectively analyzed. The dataset, gathered between 2021 and 2023, comprises 38 laboratory tests obtained from the Future Lab Pioneer database. The study's inclusion criteria focused on diabetes profile tests (glycated hemoglobin [HbA1c] and fasting blood glucose [FBG]) and manually computed inflammatory markers (NLR and MLR), which were stratified by age and sex. Results: This study demonstrated significant differences in NLR levels compared with FBG levels across all adult age groups and adult female participants (p < 0.0001), as well as among all elderly age groups ( p = 0.0006) and elderly women ( p = 0.01). MLR levels were significant in all adult age groups ( p = 0.04) and in adult women ( p = 0.02). When NLR and MLR were compared to HbA1c levels, a significant difference in the mean NLR was found in adult women ( p = 0.005). Additionally, the mean MLR levels were significant in all adult age groups ( p = 0.04) and adult women ( p = 0.02). Conclusion: Although a larger sample size is necessary for this research, the results indicate that NLR and MLR could serve as valuable indicators for evaluating inflammation in people with disrupted glucose metabolism, particularly in adult and female populations.

The increasing demand for natural food preservatives has intensified research into indigenous microorganisms with biopreservative properties. This study investigated the antimicrobial efficacy and biopreservative potential of lactic acid bacteria (LAB) isolated from traditionally fermented Dacryodes edulis seeds. From 187 initial isolates, 45 LAB strains were comprehensively characterized across three agroecological zones in Kogi State, Nigeria. Molecular identification using 16S rRNA gene sequencing revealed five species, with Lactiplantibacillus plantarum predominating (42.2%). Novel strain DE‐LAB‐23 demonstrated exceptional antimicrobial activity against Escherichia coli O157:H7 (inhibition zone: 28.4 ± 1.2 mm), Salmonella Typhimurium (26.8 ± 0.9 mm), and Listeria monocytogenes (24.6 ± 1.1 mm). Multi‐omics validation (RNA‐seq, mass spectrometry, Western blot) confirmed production of novel bacteriocins with remarkable thermal stability (85% activity at 100°C for 15 min) and broad pH tolerance (pH 3.0–9.0). Whole‐genome sequencing revealed four novel biosynthetic gene clusters encoding previously uncharacterized antimicrobial peptides. Application in a traditional African porridge model achieved 3.2‐log pathogen reduction and 72‐h shelf‐life extension at ambient temperature. These findings establish indigenous D. edulis ‐derived LAB as promising natural biopreservatives for sustainable food safety enhancement in West African traditional foods.