Background: The promotion of professional well-being improves health literacy. Objective: To identify the well-being perceived by health professionals, associated factors, and change strategies. Methodology: Qualitative, exploratory-descriptive study, applying a form with open questions. Content analysis was performed. Results: 32 professionals working in the operating room were mostly satisfied with their well-being at work. Positive factors: good environment, compatible schedules. Negative factors: management of human, material, and team resources. Strategies for change: Management of material, organizational resources, and mental health promotion strategies. Conclusions: Health professionals are generally satisfied with their level of well-being; however, some aspects can be improved.