Dr. Sergey Alferov

Research Center BioChemTech, Tula State University, Tula, Tul’skaya Oblast’, Russia

Dr. Sergey Alferov

Research Center BioChemTech, Tula State University, Tula, Tul’skaya Oblast’, Russia

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Academic Career Length

21Years (2006-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 May 2015 in Biosensors and Bioelectronics

Journal Article

Yeast-based self-organized hybrid bio-silica sol–gels for the design of biosensors

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V A Alferov

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Andrey Machulin

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T V Rogova

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8 January 2011 in Applied Biochemistry and Microbiology

Journal Article

Microbial biosensors for detection of biological oxygen demand (a Review)

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Vyacheslav Arlyapov

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Anatoly Reshetilov

The review briefs recent advances in application of biosensors for determining biological oxygen demand (BOD) in water. Special attention is focused on the principles of operation of microbial BOD...

1 September 2013 in Enzyme and Microbial Technology

Journal Article

BOD biosensor based on the yeast Debaryomyces hansenii immobilized in poly(vinyl alcohol) modified by N-vinylpyrrolidone

Vyacheslav Arlyapov

NY

N Yu Yudina

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L D Asulyan

SA

VA

V A Alferov

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Anatoly Reshetilov

An amperometric biosensor for assessing the biochemical oxygen demand (BOD) was formed by immobilizing Debaryomyces hansenii VKM Y-2482 yeast cells in poly(vinyl alcohol) modified by N-vinylpyrrolidone. Modification provided for a high sensitivity and stability of the bioreceptor. A high oxidative activity of the receptor element and the absence of any toxic effect of assayed compounds were shown for 34 substrates (alcohols, carbohydrates, carboxylic acids, amino acids, nitrophenols and surfactants) that may occur in wastewaters. Estimates of the measurement range and region of the linear dependence of signals on the BOD level, pH and temperature sensitivities, dependences of signals on concentrations of salts, stability, Michaelis kinetic constants and assay rates were obtained. The BOD values determined by the biosensor in assayed wastewater samples were shown to have a high correlation with those obtained by the standard dilution method.

1 October 2015 in Enzyme and Microbial Technology

Journal Article

A yeast co-culture-based biosensor for determination of waste water contamination levels

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N.YU. Yudina

Vyacheslav Arlyapov

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M.A. Chepurnova

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AR

Anatoly Reshetilov

1 March 2017 in Enzyme and Microbial Technology

Journal Article

Use of one- and two-mediator systems for developing a BOD biosensor based on the yeast Debaryomyces hansenii

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A.S. Zaitseva

Vyacheslav Arlyapov

NY

N.YU. Yudina

SA

AR

Anatoly Reshetilov

8 August 2022 in Microorganisms

Journal Article

Hydrocarbons Biodegradation by Rhodococcus: Assimilation of Hexadecane in Different Aggregate States

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Lenar I Akhmetov

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Ponamoreva Olga

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The aim of our study was to reveal the peculiarities of the adaptation of rhodococci to hydrophobic hydrocarbon degradation at low temperatures when the substrate was in solid states. The ability of actinobacteria Rhodococcus erythropolis (strains X5 and S67) to degrade hexadecane at 10 °C (solid hydrophobic substrate) and 26 °C (liquid hydrophobic substrate) is described. Despite the solid state of the hydrophobic substrate at 10 °C, bacteria demonstrate a high level of its degradation (30–40%) within 18 days. For the first time, we show that specialized cellular structures are formed during the degradation of solid hexadecane by Rhodococcus at low temperatures: intracellular multimembrane structures and surface vesicles connected to the cell by fibers. The formation of specialized cellular structures when Rhodococcus bacteria are grown on solid hexadecane is an important adaptive trait, thereby contributing to the enlargement of a contact area between membrane-bound enzymes and a hydrophobic substrate.

About Me

Sergey Valerievich Alferov holds a PhD in biotechnology. He is the head of the Laboratory of Ecological and Medical Biotechnology. From 2021 to the present, he has been the head of the Laboratory of Ecological and Medical Biotechnology, Tula State University, Tula, Russia. From 2011 to 2021, he was an associate professor at the Department of Biotechnology, Tula State University, Tula, Russia. From 2007 to 2008, he was a project assistant at the Biochemistry Department, Lund University, Sweden. From 2006 to 2011, he was an assistant at the Chemistry Department, Tula State University, Tula, Russia. From 2004 to 2007, he was a laboratory assistant at the Laboratory of Biosensors, G.K. Skriabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow region. From 2006 to 2010, he earned a PhD from the Pushchino State University Department of Microbiology and Biotechnology. From 2004 to 2006, he completed two years of study in post-university courses (magistracy) at the Pushchino State University Department of Microbiology and Biotechnology, Pushchino, Moscow Region, Russia. He holds a master's degree in biology. From 1999 to 2004, he completed five years of study at the Tula State University Chemistry Department, Tula, Russia. He is a specialist in chemistry. Email: s.v.alferov@gmail.com and s.v.alferov@tsu.tula.ru. ORCID: 0000-0002-5217-7815. Scopus author ID: 56585795000. Researcher ID: E-7049-2014.

Sergey Valerievich Alferov holds a PhD in biotechnology. He is the head of the Laboratory of Ecological and Medical Biotechnology. From 2021 to the present, he has been the head of the Laboratory of Ecological and Medical Biotechnology, Tula State University, Tula, Russia. From 2011 to 2021, he was an associate professor at the Department of Biotechnology, Tula State University, Tula, Russia. From 2007 to 2008, he was a project assistant at the Biochemistry Department, Lund University, Sweden. From 2006 to 2011, he was an assistant at the Chemistry Department, Tula State University, Tula, Russia. From 2004 to 2007, he was a laboratory assistant at the Laboratory of Biosensors, G.K. Skriabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow region. From 2006 to 2010, he earned a PhD from the Pushchino State University Department of Microbiology and Biotechnology. From 2004 to 2006, he completed two years of study in post-university courses (magistracy) at the Pushchino State University Department of Microbiology and Biotechnology, Pushchino, Moscow Region, Russia. He holds a master's degree in biology. From 1999 to 2004, he completed five years of study at the Tula State University Chemistry Department, Tula, Russia. He is a specialist in chemistry. Email: s.v.alferov@gmail.com and s.v.alferov@tsu.tula.ru. ORCID: 0000-0002-5217-7815. Scopus author ID: 56585795000. Researcher ID: E-7049-2014.