Inulin is a natural polysaccharide classified as a soluble fiber with demonstrated prebiotic activity. Prebiotics can reduce intestinal and systemic inflammation through modulation of the gut microflora and their metabolites. Additionally, extensive research is illuminating the role of macrophages in the interaction between gut microbiota and many systemic inflammatory diseases. In this study, the anti-inflammatory properties of inulin were evaluated using a murine macrophage cell model (RAW 264.7) of inflammation, and the immunomodulatory mechanism was investigated using omics technologies. The cells underwent comprehensive transcriptomic and proteomic analyses to identify the mechanisms responsible for the observed anti-inflammatory phenotype. Functional analyses of these omics results revealed two potential mechanisms that may lead to an overall reduction in cytokine and chemokine transcription: the inhibition of the NF-κB signaling pathway, leading to the downregulation of proinflammatory factors such as COX2, and the promotion of the phase II defense protein Hmox1 via the Nrf2 pathway. This study provides promising targets for research on immune modulation by dietary fibers and offers new strategies for the design of functional ingredients, foods, and nutraceutical products, which could ultimately lead to personalized nutrition and improved consumer health.

Grapevine trunk diseases (GTDs) are one of the most important groups of fungal diseases affecting grapevine plants worldwide. One of the main causes of GTDs infection occur during nursery plant production processes. The phytosanitary status was determined for 150 young grapevine plants (two varieties grafted onto different rootstocks) that were produced in three European nurseries. Some plants were analyzed upon submission, while others were assessed after up to 12 months growth in a greenhouse. Fungal species associated with GTDs were identified and characterized from the scions, graft unions, rootstocks, or roots. A total of 449 fungal isolates associated with GTDs were obtained, and 20 species were identified by morphological characteristics and DNA analyses. Five species were involved in Botryosphaeria dieback, six in black foot disease, six in Diaporthe dieback, and three in Petri disease. Incidence of GTDs on grapevine plants was between 81 and 100%, with different diseases varying between rootstocks and grapevine varieties. Isolates of other fungal genera not involved in the GTD complex were also detected, including Colletotrichum, Fusarium, and Rhizoctonia. The high presence of GTDs during nursery production of grapevine plants raises the need to implement effective control methods that could prevent the spread of these diseases to vineyards.

Over the past decade, there has been a growing interest in common octopus aquaculture, prompted by several factors such as the increase in market demand, the decline in overall fisheries and the search for more sustainable food resources. Nevertheless, this interest has raised concerns about the potential impact of large-scale production and intensified farming practices in the future. This study aims to establish a baseline understanding of the natural microbial communities that inhabit skin mucus of the common octopus, describe its core microbiota, and assess the impact of captive rearing on these communities and animal welfare. The skin microbiome of wild-captured octopuses was compared with that of reared in aquaculture using 16S ribosomal RNA metabarcoding. The findings show that the core microbiota of octopus mucosal skin is dominated by the phyla Bacteroidota and Pseudomonadota, with Aurantivirga, Pseudofulvibacter, and Rubritalea being the most abundant genera. Despite differences in abundance, the microbiota composition and diversity between wild and aquaculture octopuses were similar. Wild samples had an enrichment of Gammaproteobacteria, including potentially pathogenic species such as Vibrio spp., Photobacterium swingsii, and Lactococcus garvieae, which were less prevalent or absent in aquaculture samples. KEGG functional pathways predictions indicated a higher enrichment in functional pathways related to xenobiotic remediation in wild samples, reflecting their need to adapt to a more variable and potentially contaminated environment. This is the first study to characterize the mucosal skin microbiome of the common octopus and to compare wild and aquaculture specimens. The results suggest that current aquaculture practices support animal welfare through the use of controlled hatchery environments and high-quality water conditions. These insights are valuable for the development of sustainable and responsible aquaculture practices, with the octopus microbiome serving as a potential biomarker for health status and animal welfare.

Over the past decade, there has been a growing interest in common octopus aquaculture, prompted by the increasing market demand, the decline in overall fisheries and the search for a more sustainable food resource. Nevertheless, this interest has raised concerns about the potential impact of large-scale production and intensified farming practices in the future. Given the growing interest in octopus aquaculture and society's increasing concern for animal welfare, this study investigates the mucosal skin microbiota of wild and captive-bred common octopuses. The primary aim is to determine if breeding in captivity affects these communities and consequently animal welfare, using 16S ribosomal RNA metabarcoding. The core microbiota of common octopus mucosal skin is composed of the phyla Bacteroidota, Proteobacteria, Campylobacterota and Verrucomicrobiota, according to our findings. Although variations in abundance were observed, wild and aquaculture octopuses had comparable microbiota composition and diversity. Gammaproteobacteria were found to be enriched in wild mucosal skin samples, and some of the species were described as potentially pathogenic. However, these species were absent or in lower abundance in aquaculture mucosal skin samples. Additionally, analysis of KEGGs predictions showed that wild mucosal skin samples had a higher overall enrichment in functional pathways, primarily associated with xenobiotic remediation pathways, compared to the aquaculture mucosal skin samples. This is the first study to characterize the mucosal skin microbiome of the common octopus and compare the microbiome of wild and aquaculture individuals of this species. The results indicate that current aquaculture practices align with animal welfare through the use of controlled hatchery environments and high-quality water conditions. This study offers valuable insights into the microbiome of the common octopus, which can serve as a biomarker for evaluating animal welfare. It also explores the implications of these findings for the development of sustainable and responsible aquaculture practices.