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Dr. Meng Wang
Beijing Research Center for Agricultural Standards and Testing

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0 Detection
0 Mycotoxins
0 Prevention Control
0 mycotoxigenic fungi
0 Mycotoxin Degradation

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Research article
Published: 11 February 2021 in Journal of Agricultural and Food Chemistry
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The metabolism of chlorogenic acid (CGA) through the intestinal tract was studied. As cadmium is a well-known toxic heavy metal, this study was carried out to investigate the comparative protective effect of CGA and its representative intestinal metabolite (3-(3-hydroxyphenyl) propionic acid, HPPA) against Cd-induced erythrocyte cytotoxicity in vitro and in vivo. We found that CGA and its intestinal metabolite appreciably prevented erythrocyte hemolysis, osmotic fragility, and oxidative stress induced by Cd. Also, we found that HPPA had a stronger protective ability than CGA against Cd-induced erythrocyte injury in vivo, such as increasing the ratio of protein kinase C from 7.7% (CGA) to 12.0% (HPPA). Therefore, we hypothesized that CGA and its microbial metabolite had protective effects against Cd-induced erythrocyte damage via multiple actions including antioxidation and chelation. For humans, CGA supplementation may be favorable for avoiding Cd-induced biotoxicity.

ACS Style

Dai Cheng; Qi Song; Yixin Ding; Qianqian Yu; Yutong Liu; Xuena Tian; Meng Wang; Guangliang Wang; Shuo Wang. Comparative Study on the Protective Effect of Chlorogenic Acid and 3-(3-Hydroxyphenyl) Propionic Acid against Cadmium-Induced Erythrocyte Cytotoxicity: In Vitro and In Vivo Evaluation. Journal of Agricultural and Food Chemistry 2021, 69, 3859 -3870.

AMA Style

Dai Cheng, Qi Song, Yixin Ding, Qianqian Yu, Yutong Liu, Xuena Tian, Meng Wang, Guangliang Wang, Shuo Wang. Comparative Study on the Protective Effect of Chlorogenic Acid and 3-(3-Hydroxyphenyl) Propionic Acid against Cadmium-Induced Erythrocyte Cytotoxicity: In Vitro and In Vivo Evaluation. Journal of Agricultural and Food Chemistry. 2021; 69 (13):3859-3870.

Chicago/Turabian Style

Dai Cheng; Qi Song; Yixin Ding; Qianqian Yu; Yutong Liu; Xuena Tian; Meng Wang; Guangliang Wang; Shuo Wang. 2021. "Comparative Study on the Protective Effect of Chlorogenic Acid and 3-(3-Hydroxyphenyl) Propionic Acid against Cadmium-Induced Erythrocyte Cytotoxicity: In Vitro and In Vivo Evaluation." Journal of Agricultural and Food Chemistry 69, no. 13: 3859-3870.

Journal article
Published: 06 January 2021 in Comprehensive Reviews in Food Science and Food Safety
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Mycotoxins are toxic compounds naturally produced by certain types of fungi. The contamination of mycotoxins can occur on numerous foodstuffs, including cereals, nuts, fruits, and spices, and pose a major threat to humans and animals by causing acute and chronic toxic effects. In this regard, reliable techniques for accurate and sensitive detection of mycotoxins in agricultural products and food samples are urgently needed. As an advanced analytical tool, surface‐enhanced Raman spectroscopy (SERS), presents several major advantages, such as ultrahigh sensitivity, rapid detection, fingerprint‐type information, and miniaturized equipment. Benefiting from these merits, rapid growth has been observed under the topic of SERS‐based mycotoxin detection. This review provides a comprehensive overview of the recent achievements in this area. The progress of SERS‐based label‐free detection, aptasensor, and immunosensor, as well as SERS combined with other techniques, has been summarized, and in‐depth discussion of the remaining challenges has been provided, in order to inspire future development of translating the techniques invented in scientific laboratories into easy‐to‐operate analytic platforms for rapid detection of mycotoxins.

ACS Style

Wenlei Zhai; Tianyan You; Xihui Ouyang; Meng Wang. Recent progress in mycotoxins detection based on surface‐enhanced Raman spectroscopy. Comprehensive Reviews in Food Science and Food Safety 2021, 20, 1887 -1909.

AMA Style

Wenlei Zhai, Tianyan You, Xihui Ouyang, Meng Wang. Recent progress in mycotoxins detection based on surface‐enhanced Raman spectroscopy. Comprehensive Reviews in Food Science and Food Safety. 2021; 20 (2):1887-1909.

Chicago/Turabian Style

Wenlei Zhai; Tianyan You; Xihui Ouyang; Meng Wang. 2021. "Recent progress in mycotoxins detection based on surface‐enhanced Raman spectroscopy." Comprehensive Reviews in Food Science and Food Safety 20, no. 2: 1887-1909.

Critical review
Published: 06 January 2021 in The Analyst
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AuNPs have been widely used in mycotoxin detection including ELISA, optical-based sensors, electrochemical-based sensors, surface-enhanced Raman scattering-based sensors, mass-based sensors and other sensors.

ACS Style

Linxia Wu; Meng Wang; Dizhe Wei. Advances in gold nanoparticles for mycotoxin analysis. The Analyst 2021, 146, 1793 -1806.

AMA Style

Linxia Wu, Meng Wang, Dizhe Wei. Advances in gold nanoparticles for mycotoxin analysis. The Analyst. 2021; 146 (6):1793-1806.

Chicago/Turabian Style

Linxia Wu; Meng Wang; Dizhe Wei. 2021. "Advances in gold nanoparticles for mycotoxin analysis." The Analyst 146, no. 6: 1793-1806.

Short communication
Published: 30 October 2020 in Journal of the Science of Food and Agriculture
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BACKGROUND Wheat is one of three major food crops in China. Alternaria species can cause spoilage of wheat with consequent mycotoxin accumulation. Alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TeA) are the most common and frequently studied mycotoxins. There are limited regulations placed on Alternaria mycotoxin concentrations worldwide due to the lack of toxicity data available. More data on the levels of mycotoxin contamination are also needed. Moreover, it is also important to safely reduce the risks of Alternaria mycotoxins. RESULTS 132 wheat samples were collected from Hebei Province, China, and analyzed for AOH, AME and TeA. TeA was found to be the predominant Alternaria mycotoxin, especially in flour samples. Studying Alternaria species that cause black point disease of wheat indicated that Alternaria alternata and Alternaria tenuissima were the dominant species. Most of the Alternaria strains studied produced more than one mycotoxin and TeA was produced at the highest concentration, which may have resulted in the high level of TeA contamination in the wheat samples. Furthermore, magnolol displayed obvious antifungal and antimycotoxigenic activities against Alternaria. This is the first report on the antimycotoxigenic activity of magnolol against Alternaria species. CONCLUSION The Alternaria mycotoxin contamination levels in wheat and wheat products from Hebei Province, China were correlated with the toxigenic capacity of the Alternaria strains colonizing the wheat. Considering its safety, magnolol could be developed as a natural fungicide in wheat, or as a natural alternative food preservative based on its strong antifungal and antimycotoxigenic activities against Alternaria strains.

ACS Style

Dongmei Jiang; Dizhe Wei; Hongtao Li; Liuqing Wang; Nan Jiang; Yunchao Li; Meng Wang. Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol. Journal of the Science of Food and Agriculture 2020, 101, 3071 -3077.

AMA Style

Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang, Yunchao Li, Meng Wang. Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol. Journal of the Science of Food and Agriculture. 2020; 101 (7):3071-3077.

Chicago/Turabian Style

Dongmei Jiang; Dizhe Wei; Hongtao Li; Liuqing Wang; Nan Jiang; Yunchao Li; Meng Wang. 2020. "Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol." Journal of the Science of Food and Agriculture 101, no. 7: 3071-3077.

Journal article
Published: 20 October 2020 in Toxins
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Alternaria alternata is an important phytopathogen causing fruit black rot and also producing a variety of mycotoxins, such as alternariol (AOH) and alternariol monomethyl ether (AME) as two main contaminants. This could lead to economic losses of agricultural products as well as human health risks. In this study, magnolol extracted from the traditional Chinese herb, Mangnoliaofficinalis, exhibited an obvious antifungal property and could completely suppress the mycelial growth at 100 μM. Morphological differences of A. alternata were observed to be significantly shrunk and wrinkled after the exposure to magnolol. Furthermore, AOH and AME were no longer produced in response to 50 μM of magnolol. To uncover the antifungal and antimycotoxigenic mechanisms, the transcriptomic profiles of A. alternata—treated with or without magnolol—were evaluated. The clustered genes responsible for AOH and AME biosynthesis were obviously less transcribed under magnolol stress and this was further confirmed by qRT-PCR. The global regulators of carbon and nitrogen utilization, such as CreA and NmrA, were significantly down-regulated and this possibly caused the reduction in mycotoxins. In addition, fatty acid β-oxidation was regarded to contribute to polyketide mycotoxin production for the supply of precursor acetyl-CoA while the expression of these related genes was inhibited. The response to magnolol led to the marked alteration of oxidative stress and the down-expression of the mitogen-activated protein kinase (MAPK) signaling pathway from the transcriptome data and the determination of peroxidase (POD), superoxide dismutase (SOD) and glutathione (GSH) assays. This above might be the very reason for the growth supression and mycotoxin production of A. alternata by magnolol. This study provides new insights into its potential as an important active ingredient for the control of A. alternata and its mycotoxins in fruits and their products.

ACS Style

Liuqing Wang; Duo Wang; Shuzhi Yuan; Xiaoyuan Feng; Meng Wang. Transcriptomic Insights into the Antifungal Effects of Magnolol on the Growth and Mycotoxin Production of Alternaria alternata. Toxins 2020, 12, 665 .

AMA Style

Liuqing Wang, Duo Wang, Shuzhi Yuan, Xiaoyuan Feng, Meng Wang. Transcriptomic Insights into the Antifungal Effects of Magnolol on the Growth and Mycotoxin Production of Alternaria alternata. Toxins. 2020; 12 (10):665.

Chicago/Turabian Style

Liuqing Wang; Duo Wang; Shuzhi Yuan; Xiaoyuan Feng; Meng Wang. 2020. "Transcriptomic Insights into the Antifungal Effects of Magnolol on the Growth and Mycotoxin Production of Alternaria alternata." Toxins 12, no. 10: 665.

Journal article
Published: 23 March 2020 in Food Chemistry
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Phenolic acids have been reported to have many biological activities, but daily intake information is scarce. In this study, the phenolic acid contents of 116 commonly consumed food in five regions of China (Beijing, Hangzhou, Wuhan, Chongqing and Guangzhou) were analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS) and dietary intakes estimated. Tea had the highest total phenolic acids (TPA) content in all regions investigated. Phenolic acids were in esterified and bound forms, and hydroxycinnamic acids accounted for more than half of TPA content, except in tea and legumes. Average TPA intake was 193.50 mg/ day at the national level but ranged from 157.09 to 263.01 mg/ day among the regions. Rice, tea, and fruits were the main contributors to dietary intakes of TPA. Also, TPA intake in the period 2009–2013 increased 31.65 mg/ day compared with 2002, largely due to increased intakes of fruits, vegetables, and legumes.

ACS Style

Huaxin Song; Liuquan Zhang; Lipeng Wu; Weisu Huang; Mengmeng Wang; Liangxiao Zhang; Yafang Shao; Meng Wang; Fang Zhang; Ziyan Zhao; Xiaohong Mei; Tao Li; Donghui Wang; Ying Liang; Jing Li; Tao Xu; Yajing Zhao; Yongheng Zhong; Qi Chen; Baiyi Lu. Phenolic acid profiles of common food and estimated natural intake with different structures and forms in five regions of China. Food Chemistry 2020, 321, 126675 .

AMA Style

Huaxin Song, Liuquan Zhang, Lipeng Wu, Weisu Huang, Mengmeng Wang, Liangxiao Zhang, Yafang Shao, Meng Wang, Fang Zhang, Ziyan Zhao, Xiaohong Mei, Tao Li, Donghui Wang, Ying Liang, Jing Li, Tao Xu, Yajing Zhao, Yongheng Zhong, Qi Chen, Baiyi Lu. Phenolic acid profiles of common food and estimated natural intake with different structures and forms in five regions of China. Food Chemistry. 2020; 321 ():126675.

Chicago/Turabian Style

Huaxin Song; Liuquan Zhang; Lipeng Wu; Weisu Huang; Mengmeng Wang; Liangxiao Zhang; Yafang Shao; Meng Wang; Fang Zhang; Ziyan Zhao; Xiaohong Mei; Tao Li; Donghui Wang; Ying Liang; Jing Li; Tao Xu; Yajing Zhao; Yongheng Zhong; Qi Chen; Baiyi Lu. 2020. "Phenolic acid profiles of common food and estimated natural intake with different structures and forms in five regions of China." Food Chemistry 321, no. : 126675.

Journal article
Published: 22 February 2020 in Toxins
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Type-B aflatoxins (AFB1 and AFB2) frequently contaminate food, especially nuts and fried figs, and seriously threaten human health; hence, it is necessary for the newly rapid and sensitive detection methods to prevent the consumption of potentially contaminated food. Here, a lateral flow aptasensor for the detection of type-B aflatoxins was developed. It is based on the use of fluorescent dye Cy5 as a label for the aptamer, and on the competition between type-B aflatoxins and the complementary DNA of the aptamer. This is the first time that the complementary strand of the aptamer has been used as the test line (T-line) to detect type-B aflatoxins. In addition, the truncated aptamer was used to improve the affinity with type-B aflatoxins in our study. Therefore, the lengths of aptamer and cDNA probe were optimized as key parameters for higher sensitivity. In addition, binding buffer and organic solvent were investigated. The results showed that the best pair for achieving improved sensitivity and accuracy in detecting AFB1 was formed by a shorter aptamer (32 bases) coupled with the probe complementary to the AFB1 binding region of the aptamer. Under the optimal experimental conditions, the test strip showed an excellent linear relationship in the range from 0.2 to 20 ng/mL with a limit of detection of 0.16 ng/mL. This aptamer-based strip was successfully applied to the determination of type-B aflatoxins in spiked and commercial peanuts, almonds, and dried figs, and the recoveries of the spiked samples were from 93.3%−112.0%. The aptamer-complementary strand-based lateral flow test strip is a potential alternative tool for the rapid and sensitive detection of type-B aflatoxins in nuts and dried figs. It is of help for monitoring aflatoxins to avoid the consumption of unsafe food.

ACS Style

Zhilei Zhao; He Wang; Wenlei Zhai; Xiaoyuan Feng; Xia Fan; Ailiang Chen; Meng Wang. A Lateral Flow Strip Based on a Truncated Aptamer-Complementary Strand for Detection of Type-B Aflatoxins in Nuts and Dried Figs. Toxins 2020, 12, 136 .

AMA Style

Zhilei Zhao, He Wang, Wenlei Zhai, Xiaoyuan Feng, Xia Fan, Ailiang Chen, Meng Wang. A Lateral Flow Strip Based on a Truncated Aptamer-Complementary Strand for Detection of Type-B Aflatoxins in Nuts and Dried Figs. Toxins. 2020; 12 (2):136.

Chicago/Turabian Style

Zhilei Zhao; He Wang; Wenlei Zhai; Xiaoyuan Feng; Xia Fan; Ailiang Chen; Meng Wang. 2020. "A Lateral Flow Strip Based on a Truncated Aptamer-Complementary Strand for Detection of Type-B Aflatoxins in Nuts and Dried Figs." Toxins 12, no. 2: 136.

Journal article
Published: 20 September 2019 in Toxins
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Alternaria alternata is a critical phytopathogen that causes foodborne spoilage and produces a polyketide mycotoxin, alternariol (AOH), and its derivative, alternariol monomethyl ether (AME). In this study, the inhibitory effects of the essential oil citral on the fungal growth and mycotoxin production of A. alternata were evaluated. Our findings indicated that 0.25 μL/mL (222.5 μg/mL) of citral completely suppressed mycelial growth as the minimum inhibitory concentration (MIC). Moreover, the 1/2MIC of citral could inhibit more than 97% of the mycotoxin amount. Transcriptomic profiling was performed by comparative RNA-Seq analysis of A. alternata with or without citral treatment. Out of a total of 1334 differentially expressed genes (DEGs), 621 up-regulated and 713 down-regulated genes were identified under citral stress conditions. Numerous DEGs for cell survival, involved in ribosome and nucleolus biogenesis, RNA processing and metabolic processes, and protein processing, were highly expressed in response to citral. However, a number of DEGs responsible for the metabolism of several carbohydrates and amino acids, sulfate and glutathione metabolism, the metabolism of xenobiotics and transporter activity were significantly more likely to be down-regulated. Citral induced the disturbance of cell integrity through the disorder of gene expression, which was further confirmed by the fact that exposure to citral caused irreversibly deleterious disruption of fungal spores and the inhibition of ergosterol biosynthesis. Citral perturbed the balance of oxidative stress, which was likewise verified by a reduction of total antioxidative capacity. In addition, citral was able to modulate the down-regulation of mycotoxin biosynthetic genes, including pksI and omtI. The results provide new insights for exploring inhibitory mechanisms and indicate citral as a potential antifungal and antimytoxigenic alternative for cereal storage.

ACS Style

Liuqing Wang; Nan Jiang; Duo Wang; Meng Wang. Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata. Toxins 2019, 11, 553 .

AMA Style

Liuqing Wang, Nan Jiang, Duo Wang, Meng Wang. Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata. Toxins. 2019; 11 (10):553.

Chicago/Turabian Style

Liuqing Wang; Nan Jiang; Duo Wang; Meng Wang. 2019. "Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata." Toxins 11, no. 10: 553.

Journal article
Published: 12 September 2019 in International Journal of Food Microbiology
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Large amounts of tomato fruits and derived products are produced in China and may be contaminated by Alternaria mycotoxins, which may have the potential risks for human health. There is thus an increasing interest in reducing the mycotoxins. In the present study, 26 Alternaria strains isolated from tomato black rots were identified according to morphological and molecular grounds, and their mycotoxigenic abilities for alternariol (AOH), alternariol monomethyl-ether (AME) and tenuazonic acid (TeA) were also investigated. The results showed that A. alternate was the predominant species with incidence values of 65.4% (17/26), followed by A. brassicae (7/26) and A. tenuissima (2/26). A. alternate isolates showed the highest capacity for AOH, AME and TeA production among the studied isolates either in vitro or in vivo, suggested that A. alternata may be the most important mycotoxin-producing species in tomato fruits. Thus, UV-C irradiation was used to reduce the mycotoxin produced by A. alternata in our study. The results showed that low dose of UV-C irradiation (0.25 kJ/m2) could effectively inhibit mycotoxins production and penetration in tomatoes. Upon treatment with UV-C, there was 79.6, 76.4 and 51.4% of reduction in AOH, AME and TeA penetration when compared to untreated fruits. This may be associated with the enhanced phenolics by UV-C irradiation. In fact, the induced phenolics were including p-coumaric, ferulic and pyrocatechuic acids, of which p-coumaric acid (1.0 mM) displayed the highest reduction of TeA with 60.2%, whereas ferulic acid (1.0 mM) showed strong inhibitory effects on the AOH and AME production by 59.4 and 79.1%, respectively. Therefore, the application of UV-C irradiation seems to be a promising method for reducing the potential risk of Alternaria mycotoxins in fruits and also for enhancing phenolics of processing products.

ACS Style

Nan Jiang; Zuliang Li; Liuqing Wang; He Li; Xuan Zhu; Xiaoyuan Feng; Meng Wang. Effects of ultraviolet-c treatment on growth and mycotoxin production by Alternaria strains isolated from tomato fruits. International Journal of Food Microbiology 2019, 311, 108333 .

AMA Style

Nan Jiang, Zuliang Li, Liuqing Wang, He Li, Xuan Zhu, Xiaoyuan Feng, Meng Wang. Effects of ultraviolet-c treatment on growth and mycotoxin production by Alternaria strains isolated from tomato fruits. International Journal of Food Microbiology. 2019; 311 ():108333.

Chicago/Turabian Style

Nan Jiang; Zuliang Li; Liuqing Wang; He Li; Xuan Zhu; Xiaoyuan Feng; Meng Wang. 2019. "Effects of ultraviolet-c treatment on growth and mycotoxin production by Alternaria strains isolated from tomato fruits." International Journal of Food Microbiology 311, no. : 108333.

Journal article
Published: 05 July 2019 in Viruses
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Characterization of viroid-derived small RNAs (vd-sRNAs) is important to understand viroid–host interactions; however, vd-sRNAs belonging to the genus Coleviroid are yet to be identified and characterized. Herein, we used coleus plants singly infected with coleus blumei viroid (CbVd)-1, -5, or -6 and doubly infected with CbVd-1 and -5 to identify and analyze their vd-sRNAs. We found sense and antisense vd-sRNAs for CbVd-1, -5 and -6, and 22-nt vd-sRNAs were the most abundant; moreover, the 5′-terminal nucleotides (nts) of CbVd-1, -5, and -6 were biased toward U and C, and sRNAs derived from these three viroids were unevenly distributed along their genomes. We also noted that CbVd-5 and -6 share a fragment that forms the right half of the rod-like secondary structure of these viroids, which implied that they generated almost the same type of vd-sRNAs. This finding indicated that vd-sRNA biogenesis is mainly determined by the primary sequence of their substrates. More importantly, we found two complementary vd-sRNAs (22 nt) that were generated from the central conserved region (CCR) of these three viroids, suggesting an important role of CCR in vd-sRNA biogenesis. In conclusion, our results provide novel insight into the biogenesis of vd-sRNAs and the biological roles of CCR.

ACS Style

Dong-Mei Jiang; Meng Wang; Shi-Fang Li; Zhi-Xiang Zhang. High-Throughput Sequencing Analysis of Small RNAs Derived from Coleus Blumei Viroids. Viruses 2019, 11, 619 .

AMA Style

Dong-Mei Jiang, Meng Wang, Shi-Fang Li, Zhi-Xiang Zhang. High-Throughput Sequencing Analysis of Small RNAs Derived from Coleus Blumei Viroids. Viruses. 2019; 11 (7):619.

Chicago/Turabian Style

Dong-Mei Jiang; Meng Wang; Shi-Fang Li; Zhi-Xiang Zhang. 2019. "High-Throughput Sequencing Analysis of Small RNAs Derived from Coleus Blumei Viroids." Viruses 11, no. 7: 619.

Evaluation study
Published: 24 June 2019 in Journal of Agricultural and Food Chemistry
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The simultaneous detection of multiple mycotoxins is important due to the increased toxic effects of combined mycotoxins in grains. In this research, a combination of modified QuEChERS with ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) was used for simultaneous detection of 20 mycotoxins in grains. A series of different types of magnetic (Fe3O4) nanoparticles modified with multiwalled carbon nanotubes (Fe3O4-MWCNTs) were designed as modified QuEChERS adsorbents for facile and efficient purification and the target interferences removing in the matrices. When there is an external magnetic field, the proposed modified QuEChERS method uses a shorter pretreatment time compared with the traditional QuEChERS method, which makes it possible to conduct high-throughput analyses. To optimize the QuEChERS process, the extraction solvent and the type and amount of the Fe3O4-MWCNTs were investigated. Under optimal conditions, the method was validated and showed satisfactory linearity (r2≥0.9965), good recovery (73.5–112.9%), good precision (1.3–12.7%), and excellent sensitivity (ranging from 0.0021 to 5.4457 ng‧g-1), which indicates that this method can be used for detecting multiple mycotoxins in real samples.

ACS Style

Shuai Ma; Meng Wang; Tianyan You; Kun Wang. Using Magnetic Multiwalled Carbon Nanotubes as Modified QuEChERS Adsorbent for Simultaneous Determination of Multiple Mycotoxins in Grains by UPLC-MS/MS. Journal of Agricultural and Food Chemistry 2019, 67, 8035 -8044.

AMA Style

Shuai Ma, Meng Wang, Tianyan You, Kun Wang. Using Magnetic Multiwalled Carbon Nanotubes as Modified QuEChERS Adsorbent for Simultaneous Determination of Multiple Mycotoxins in Grains by UPLC-MS/MS. Journal of Agricultural and Food Chemistry. 2019; 67 (28):8035-8044.

Chicago/Turabian Style

Shuai Ma; Meng Wang; Tianyan You; Kun Wang. 2019. "Using Magnetic Multiwalled Carbon Nanotubes as Modified QuEChERS Adsorbent for Simultaneous Determination of Multiple Mycotoxins in Grains by UPLC-MS/MS." Journal of Agricultural and Food Chemistry 67, no. 28: 8035-8044.

Journal article
Published: 10 October 2018 in Toxins
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One-step solid-phase extraction (SPE) using a multiwalled carbon nanotube (MWCNT) for simultaneous analysis of 21 mycotoxins, including nine trichothecenes, zearalenone (ZEN) and its derivatives, four aflatoxins, and two ochratoxins, in corn and wheat was developed. Several key parameters affecting the performance of the one-step SPE procedure—types of MWCNT, combinations with five sorbents (octadecylsilyl (C18), hydrophilic–lipophilic balance (HLB), mixed-mode cationic exchange (MCX), silica gel, and amino-propyl (NH2)), and filling amounts of the MWCNTs—were thoroughly investigated. The combination of 20 mg carboxylic MWCNT and 200 mg C18 was proven to be the most effective, allowing the quantification of all analyzed mycotoxins in corn and wheat. Under the optimized cleanup procedure prior to ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) analysis, the method was validated by analyzing samples spiked at the limit of quantification (LOQ), two-times LOQ, and 10-times LOQ. Satisfactory linearity (r2 ≥ 0.9910), high sensitivity (LOQ in different ranges of 0.5–25 μg L−1), good recovery (75.6–110.3%), and acceptable precision (relative standard deviation (RSD), 0.3–10.7%) were obtained. The applicability of the method was further confirmed using raw samples of corn and wheat. In conclusion, the established method was rapid, simple and reliable for simultaneous analysis of 21 mycotoxins in corn and wheat.

ACS Style

Dongmei Jiang; Dizhe Wei; Liuqing Wang; Shuai Ma; Yuanfang Du; Meng Wang. Multiwalled Carbon Nanotube for One-Step Cleanup of 21 Mycotoxins in Corn and Wheat Prior to Ultraperformance Liquid Chromatography–Tandem Mass Spectrometry Analysis. Toxins 2018, 10, 409 .

AMA Style

Dongmei Jiang, Dizhe Wei, Liuqing Wang, Shuai Ma, Yuanfang Du, Meng Wang. Multiwalled Carbon Nanotube for One-Step Cleanup of 21 Mycotoxins in Corn and Wheat Prior to Ultraperformance Liquid Chromatography–Tandem Mass Spectrometry Analysis. Toxins. 2018; 10 (10):409.

Chicago/Turabian Style

Dongmei Jiang; Dizhe Wei; Liuqing Wang; Shuai Ma; Yuanfang Du; Meng Wang. 2018. "Multiwalled Carbon Nanotube for One-Step Cleanup of 21 Mycotoxins in Corn and Wheat Prior to Ultraperformance Liquid Chromatography–Tandem Mass Spectrometry Analysis." Toxins 10, no. 10: 409.

Research article
Published: 03 February 2018 in Journal of Agricultural and Food Chemistry
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Phytosterols are well-known for their cholesterol-lowering effects, and the structures and forms of phytosterols affect their bioactivity. We aimed to illustrate the phytosterol profiles in common foods and estimate their natural intake in five geographical regions and among different age groups in China. In total, 12 phytosterols in free and esterified forms of 119 foods from five regions across China were examined using gas chromatography–mass spectrometry. Then, the dietary intake of phytosterols was calculated combined with the dietary foods intake data of Chinese people. The total phytosterol content was highest in vegetable oils (150.4–1230.9 mg/100 g), followed by legumes (129.6–275.6 mg/100 g), nuts (18.9–255.2 mg/100 g), and cereals (11.9–93.8 mg/100 g). Vegetables and fruits contained lower contents of total phytosterols. Phytosterols were mainly esterified in most common foods except in nuts. The predominant phytosterols were β-sitosterol, campesterol, and stigmasterol, all of which belonged to plant sterols and 4-desmethylsterols. Total phytosterol intake varied across different regions, ranging between 257.7 and 473.7 mg/standard-person (sp)/day, with the highest intake in Beijing, followed by Hangzhou, Wuhan, Chongqing, and Guangzhou. However, phytosterol proportion was similar across regions, with β-sitosterol accounting for 46.5–50.3% of the natural intake. Phytosterol intake was mainly constituted by plant sterols and 4-desmethylsterols in esterified form (61.9–74.6%). At the age of 2–70 years, phytosterol intake ranged from 154.3 mg/day to 348.0 mg/day in the national scale.

ACS Style

Mengmeng Wang; Weisu Huang; Yinzhou Hu; Liangxiao Zhang; Ya-Fang Shao; Meng Wang; Fang Zhang; Ziyan Zhao; Xiaohong Mei; Tao Li; Donghui Wang; Ying Liang; Jing Li; Yining Huang; Liuquan Zhang; Tao Xu; Huaxin Song; Yongheng Zhong; Baiyi Lu. Phytosterol Profiles of Common Foods and Estimated Natural Intake of Different Structures and Forms in China. Journal of Agricultural and Food Chemistry 2018, 66, 2669 -2676.

AMA Style

Mengmeng Wang, Weisu Huang, Yinzhou Hu, Liangxiao Zhang, Ya-Fang Shao, Meng Wang, Fang Zhang, Ziyan Zhao, Xiaohong Mei, Tao Li, Donghui Wang, Ying Liang, Jing Li, Yining Huang, Liuquan Zhang, Tao Xu, Huaxin Song, Yongheng Zhong, Baiyi Lu. Phytosterol Profiles of Common Foods and Estimated Natural Intake of Different Structures and Forms in China. Journal of Agricultural and Food Chemistry. 2018; 66 (11):2669-2676.

Chicago/Turabian Style

Mengmeng Wang; Weisu Huang; Yinzhou Hu; Liangxiao Zhang; Ya-Fang Shao; Meng Wang; Fang Zhang; Ziyan Zhao; Xiaohong Mei; Tao Li; Donghui Wang; Ying Liang; Jing Li; Yining Huang; Liuquan Zhang; Tao Xu; Huaxin Song; Yongheng Zhong; Baiyi Lu. 2018. "Phytosterol Profiles of Common Foods and Estimated Natural Intake of Different Structures and Forms in China." Journal of Agricultural and Food Chemistry 66, no. 11: 2669-2676.

Journal article
Published: 01 November 2017 in Molecules
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Phenolic acids are thought to be beneficial for human health and responsible for vegetables’ health-promoting properties. Free, conjugated, and bound phenolic acids of seven commonly consumed vegetables, including kidney bean, cow pea, snow pea, hyacinth bean, green soy bean, soybean sprouts and daylily, from the regions of Beijing, Hangzhou, and Guangzhou, were identified and quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Three vegetables, namely green soy bean, soybean sprouts, and daylily (Hemerocallis fulva L.), from the Beijing region contained higher concentrations of total phenolic acids than those from the Hangzhou and Guangzhou regions. The results indicated that the phenolic acid content in the seven vegetables appeared to be species-dependent. The highest content of phenolic acids was found in daylily, followed by green soy bean, while the least amounts were identified in kidney bean and hyacinth bean. Typically, phenolic acids are predominantly found in conjugated forms. Principle component analysis (PCA) revealed some key compounds that differentiated the seven vegetables. Green soy bean, compared to the other six vegetables, was characterized by higher levels of syringic acid, ferulic acid, vanillic acid, and sinapic acid. Other compounds, particularly p-coumaric acid, neochlorogenic acid, and caffeic acid, exhibited significantly higher concentrations in daylily. In addition, p-coumaric acid was the characteristic substance in cow pea. Results from this study can contribute to the development of vegetables with specific phytochemicals and health benefits.

ACS Style

Yuan Gao; Shuai Ma; Meng Wang; Xiao-Yuan Feng. Characterization of Free, Conjugated, and Bound Phenolic Acids in Seven Commonly Consumed Vegetables. Molecules 2017, 22, 1878 .

AMA Style

Yuan Gao, Shuai Ma, Meng Wang, Xiao-Yuan Feng. Characterization of Free, Conjugated, and Bound Phenolic Acids in Seven Commonly Consumed Vegetables. Molecules. 2017; 22 (11):1878.

Chicago/Turabian Style

Yuan Gao; Shuai Ma; Meng Wang; Xiao-Yuan Feng. 2017. "Characterization of Free, Conjugated, and Bound Phenolic Acids in Seven Commonly Consumed Vegetables." Molecules 22, no. 11: 1878.

Journal article
Published: 26 June 2017 in Toxins
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Occurrence of toxigenic molds and mycotoxins on dried fruits is a worldwide problem, but limited information is available in China. A total of 220 dried fruits (raisins, dried apricots, dates and wolfberries) purchased from China were analyzed for 17 mycotoxins (i.e., Alternaria toxins, ochratoxin A (OTA), patulin (PAT) and trichothecenes) by UPLC-MS/MS, combined with a single-step cleanup. The result showed that at least one mycotoxin was detected in 142 samples (64.6%). The lowest incidence of contaminated samples was observed in dried apricots (48.2%), and the highest incidence in dried wolfberries (83.3%). The Alternaria toxins seemed to be the major problem in dried fruits, rather than OTA or PAT. Tenuazonic acid (TeA) was the predominant mycotoxin, in both frequency and concentration, ranging from 6.9 to 5665.3 μg kg−1, followed by tentoxin (TEN; 20.5%), and mycophenolic acid (MPA; 19.5%). Moreover, raisins are more likely to be contaminated with OTA than the other dried fruits. Penicillic acid (PA) was detected only in dried dates, and PAT was detected only in one apricot sample. In addition, our results also showed that the simultaneous presence of 2–4 mycotoxins was observed in 31.4% of dried fruits. TeA and TEN were the most frequent combination, detected in 29 (13.2%) samples, followed by TeA and MPA with a prevalence of 11.4%. Therefore, the results of this survey suggest the need for wider monitoring on the contamination of these mycotoxins, especially Alternaria toxins in agro-products, and indicate the importance of setting a maximum limit for Alternaria toxins in China.

ACS Style

Dizhe Wei; Yao Wang; Dongmei Jiang; Xiaoyuan Feng; Jun Li; Meng Wang. Survey of Alternaria Toxins and Other Mycotoxins in Dried Fruits in China. Toxins 2017, 9, 200 .

AMA Style

Dizhe Wei, Yao Wang, Dongmei Jiang, Xiaoyuan Feng, Jun Li, Meng Wang. Survey of Alternaria Toxins and Other Mycotoxins in Dried Fruits in China. Toxins. 2017; 9 (7):200.

Chicago/Turabian Style

Dizhe Wei; Yao Wang; Dongmei Jiang; Xiaoyuan Feng; Jun Li; Meng Wang. 2017. "Survey of Alternaria Toxins and Other Mycotoxins in Dried Fruits in China." Toxins 9, no. 7: 200.

Research article
Published: 23 June 2017 in Journal of Agricultural and Food Chemistry
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Early and late ripening sweet cherries were characterized for phenolic acids, and also their antioxidant capacity and potential antifungal effects were investigated. Free, conjugated, and bound phenolics were identified and quantified using ultra performance liquid chromatography–tandem mass spectrometry. Our results indicated that the early ripening cultivars contained higher free phenolic acids, which was positively related to remarkable antioxidant properties and the inhibition effects on Alternaria alternata and tenuazonic acid (TeA) accumulation. However, conjugated phenolics of the late ripening cultivars, mainly including caffeic, 2,3,4-trihydroxybenzoic, p-coumaric, and pyrocatechuic acids, achieved the highest antifungal effects and almost completely inhibited the A. alternata and TeA production. 2,2-Diphenyl-1-picrylhydrazyl testing and ferric ion reducing antioxidant power assay showed strong positive correlation with total phenolics and specific phenolics such as free epicatechin and conjugated 2,3,4-trihydroxybenzoic acids and also with antifungal activity. Results from this study provide further insights into the health-promoting phenolic compounds in sweet cherries.

ACS Style

Meng Wang; Nan Jiang; Yao Wang; Dongmei Jiang; Xiaoyuan Feng. Characterization of Phenolic Compounds from Early and Late Ripening Sweet Cherries and Their Antioxidant and Antifungal Activities. Journal of Agricultural and Food Chemistry 2017, 65, 5413 -5420.

AMA Style

Meng Wang, Nan Jiang, Yao Wang, Dongmei Jiang, Xiaoyuan Feng. Characterization of Phenolic Compounds from Early and Late Ripening Sweet Cherries and Their Antioxidant and Antifungal Activities. Journal of Agricultural and Food Chemistry. 2017; 65 (26):5413-5420.

Chicago/Turabian Style

Meng Wang; Nan Jiang; Yao Wang; Dongmei Jiang; Xiaoyuan Feng. 2017. "Characterization of Phenolic Compounds from Early and Late Ripening Sweet Cherries and Their Antioxidant and Antifungal Activities." Journal of Agricultural and Food Chemistry 65, no. 26: 5413-5420.

Journal article
Published: 29 April 2017 in Toxins
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A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1–10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit.

ACS Style

Yan Man; Gang Liang; Fuchao Jia; An Li; Hailong Fu; Meng Wang; Ligang Pan. Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit. Toxins 2017, 9, 152 .

AMA Style

Yan Man, Gang Liang, Fuchao Jia, An Li, Hailong Fu, Meng Wang, Ligang Pan. Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit. Toxins. 2017; 9 (5):152.

Chicago/Turabian Style

Yan Man; Gang Liang; Fuchao Jia; An Li; Hailong Fu; Meng Wang; Ligang Pan. 2017. "Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit." Toxins 9, no. 5: 152.