Multi-story, reinforced-concrete (RC) building structures with soft stories are highly vulnerable to damage due to earthquake loads. The soft story causes a significant stiffness irregularity, which has led to numerous buildings collapsing in previous seismic events. In addition to the structural collapse, the failure of non-structural components (NSCs) has also been observed during past earthquakes. In light of this, this study investigates the effect of a soft story and its location on the seismic behavior of a supporting building and NSCs. The soft story is assumed to be located on the bottom (ground), middle, and top-story levels of the considered building models. Story displacements and inter-story drift ratios are evaluated to assess structural behavior. The floor response spectra and the amplification effects of NSC on the floor acceleration responses are studied to understand the behavior of NSCs. The analysis results revealed that the bottom soft story exhibits a considerable vertical stiffness irregularity, and its position substantially affects the floor response spectra. The amplification in the floor acceleration response was found to be greater at the soft-story level. This study reported that middle soft-story buildings exhibit the most remarkable amplification in the component’s acceleration. Finally, peak floor response demands are compared with the code-based formulation, and it is found that the code-based formulation’s linear assumption may lead peak floor response demands to be underestimated or overestimated.