Desertification in semi-arid regions is currently a global environmental and societal problem. This research attempts to understand whether a 40-year-old rain-fed Artemisia sphaerocephala Krasch sand-fixing land as part of the Three North Shelterbelt Program (3NSP) of China can be developed sustainably or not using a newly designed lysimeter to monitor the precipitation-induced deep soil recharge (DSR) at 220 cm of depth. Evapotranspiration is calculated through a water balance equation when precipitation and soil moisture data are collected. A comparison of soil particle sizes and soil moisture distributions in artificial sand-fixing land and neighboring bare land is made to assess the impact of sand-fixing reforestation. Results show that such a sand-fixing reforestation results in a root system being mainly developed in the horizontal direction and a changed soil particle distribution. Specifically, the sandy soil with 50.53 % medium sand has been transformed into a sandy soil with 68.53 % fine sand. Within the Artemisia sphaerocephala Krasch sand-fixing experimental area, the DSR values in the bare sand plot and Artemisia sphaerocephala Krasch plot are respectively 283.6 and 90.6 mm in wet years, reflecting a difference of more than 3 times. The deep soil layer moisture in semi-arid sandy land is largely replenished by precipitation-induced infiltration. The DSR values of the bare sandy land plot and Artemisia sphaerocephala Krasch plot are respectively 51.6 and 2 mm in dry years, a difference of more than 25 times. The proportions of DSR reduced by Artemisia sphaerocephala Krasch are 68.06 % and 96.12 % in wet and dry years, respectively. This research shows that Artemisia sphaerocephala Krasch in semi-arid regions can continue to grow and has the capacity to fix sand. It consumes a large amount of precipitated water and reduces the amount of DSR considerably.

Background: China’s so-called Three North Shelterbelt Program (3NSP) has produced a vast area of lined forest reconstruction in the semi-arid regions. This study uses the lined rain-fed Pinus sylvestris var. mongolica (PSM) sand-fixing forest in the eastern part of Mu Us Sandy Land in Northwestern China as an example to investigate the ecohydrological process in this region. Rain gauges, newly designed lysimeters and soil moisture sensors are used to monitor precipitation, deep soil recharge (DSR) and soil water content, where DSR specifically refers to recharge that can reach a depth more than 200 cm and eventually replenish the underneath groundwater reservoir.Results: This study shows that there are two obvious moisture recharge processes in an annual base for the PSM forest soil: a snowmelt-related recharge process in the spring and a precipitation-related recharge process in the summer. The recharge depth of the first process can reach 180 cm without DSR occurring (in 2018). The second process results in noticeable DSR in 2018. Specifically, the DSR values over 2016–2018 are 1, 0.2, and 1.2 mm, respectively. To reach the recharge depths of 20, 40, 80, 120, 160, and 200 cm, the required precipitation intensities have to be 2.6, 3.2, 3.4, 8.2, 8.2, and 13.2 mm/d, respectively. The annual evapotranspiration in the PSM forest is 466.94 mm in 2016, 324.60 mm in 2017, and 183.85 mm in 2018.Conclusion: This study concludes that under the current precipitation conditions (including both dry- and wet-years such as 2016–2018), water consumption of PSM somewhat equals to the precipitation amount, and PSM has evolved over years to regulate its evapotranspiration in response to annual precipitation fluctuations in Mu Us Sandy Land of China.

The calcineurin B-like-interacting protein kinase (CIPK) protein family plays a key role in the plant calcium ion-mediated signal transduction pathway, which regulates a plant’s response to abiotic stress. Nitraria sibirica pall. (N. sibirica) is a halophyte with a strong tolerance for high salt environments, yet how it is able to deal with salt stress on a molecular level is still unknown. Due to their function as described in other plant species, CIPK genes are prime candidates for a role in salt stress signaling in N. sibirica. In this study, we identified and analyzed the phylogenetic makeup and gene expression of the N. sibirica CIPK gene family. A total of 14 CIPKs were identified from the N. sibirica genome and were clustered into seven groups based on their phylogeny. The promoters of NsCIPK genes contained multiple elements involved in hormonal and stress response. Synteny analysis identified a total of three pairs of synteny relationships between NsCIPK genes. Each gene showed its own specific expression pattern across different tissues, with the overall expression of CIPK6 being the lowest, and that of CIPK20 being the highest. Almost all CIPK genes tended to respond to salt, drought, and cold stress, but with different sensitivity levels. In this study, we have provided a general description of the NsCIPK gene family and its expression, which will be of great significance for further understanding of the NsCIPK gene family function.

Desert farmland provides food for desert areas, but water is the main limiting factor of this region, thus desert farmland has an extremely fragile ecological environment. This study investigated the temporal and spatial variations of vegetation NDVI (Normalized Difference Vegetation Index) in the Ulan Buh Desert, China, from 1990 to 2022, using long-term Landsat satellite data obtained from the Google Earth Engine platform and local statistical data. The results showed that from 1990 to 2022, the NDVI exhibited relatively small fluctuations and a steady increase. Furthermore, the study analyzed the impact of climate factors, namely precipitation and temperature, on NDVI, and collected the groundwater lever changes under irrigation and farmland development. The results demonstrated a positive correlation between NDVI and both precipitation and temperature from 1990 to 2006. The study area experienced an overall trend of increasing humidity. Specifically, from 1990 to 2006, significant positive correlations with precipitation and temperature were observed in 4.4% and 5.5% of the region, respectively. From 2007 to 2022, significant positive correlations were observed in 5.4% and 72.8% of the region for precipitation and temperature, respectively. These findings suggest that temperature has become increasingly influential on vegetation NDVI, while the impact of precipitation remains relatively stable. Moreover, the study assessed the impact of human activities on vegetation NDVI. The results revealed that from 1990 to 2006, human activities contributed to 43.1% of the promotion of local vegetation NDVI, which increased to 90.9% from 2007 to 2022. This study provides valuable insights into the dynamics of vegetation in the Ulan Buh Desert and its response to climatic changes and human activities. The findings highlight the significance of climate conditions and human interventions in shaping the vegetation dynamics in the region, offering essential information for ecological restoration and conservation efforts.

Desert ecosystem CO₂ exchange may play an important role in global carbon cycling. However, it is still not clear how the CO₂ fluxes of shrub-dominated desert ecosystems respond to precipitation changes. We performed a 10-year long-term rain addition experiment in a Nitraria tangutorum desert ecosystem in northwestern China. In the growing seasons of 2016 and 2017, with three rain addition treatments (natural precipitation +0%, +50%, and +100% of annual average precipitation), gross ecosystem photosynthesis (GEP), ecosystem respiration (ER), and net ecosystem CO₂ exchange (NEE) were measured. The GEP responded nonlinearly and the ER linearly to rain addition. The NEE presented a nonlinear response along the rain addition gradient, with a saturation threshold by rain addition between +50% and +100%. The growing season mean NEE ranged from −2.25 to −5.38 μmol CO₂ m⁻² s⁻¹, showing net CO₂ uptake effect, with significant enhancement (more negative) under the rain addition treatments. Although natural rainfall fluctuated greatly in the growing seasons of 2016 and 2017, reaching 134.8% and 44.0% of the historical average, the NEE values remained stable. Our findings highlight that growing season CO₂ sequestration in desert ecosystems will increase against the background of increasing precipitation levels. The different responses of GEP and ER of desert ecosystems under changing precipitation regimes should be considered in global change models.

Alfalfa (Medicago satiua L.) is a major forage legume in semi-arid regions such as North China Plain and is the material foundation for the development of herbivorous animal husbandry. How to improve the yield of alfalfa per unit area from a technical perspective and achieve high-yield cultivation of alfalfa is the focus of research by scientific researchers and producers. To evaluate the effects of irrigation and P fertilization as well as the P residual effect on alfalfa yield, we conducted a six-year (2008–2013) field experiment in loamy sand soil. There were four irrigation levels (W0: 0 mm, W1: 25 mm, W2: 50 mm, W3: 75 mm per time, four times a year) and three P fertilization levels (F0: 0 kg P₂O₅ ha⁻¹, F1: 52.5 kg P₂O₅ ha⁻¹, F2: 105 kg P₂O₅ ha⁻¹ per time, twice a year). The highest dry matter yield (DMY) was obtained in the W2F2 treatment, with an annual mean of 13,961.1 kg ha⁻¹. During 2009–2013, the DMY of first and second-cut alfalfa increased significantly with increasing irrigation levels, whereas the opposite pattern was observed in fourth-cut alfalfa. Regression analysis revealed that the optimal amount of water supply (sum of seasonal irrigation and rainfall during the growing season) to obtain maximum DMY was between 725 and 755 mm. Increasing P fertilization contributed to significantly higher DMY in each cut of alfalfa during 2010–2013 but not in the first two growing seasons. The mean annual DMY of W0F2, W1F2, W2F2, and W3F2 treatments was 19.7%, 25.6%, 30.7%, and 24.1% higher than that of W0F0 treatment, respectively. When no P fertilizer was applied in F2 plots in 2013, soil availability and total P concentrations, annual alfalfa DMY, and plant nutrient contents did not differ significantly compared with those in fertilized F2 plots. Results of this study suggest that moderate irrigation with lower annual P fertilization is a more environmentally sound management practice while maintaining alfalfa productivity in the semi-arid study area.