Cocoa (Theobroma cacao L.) is one of the most widely consumed edible seeds in the world affected by on-farm processing. This study investigated the effect of different drying techniques, namely oven drying (OD), sun drying (SD), and a modification of sun drying using black plastic sheeting (SBPD), on the volatile profile of fine-flavor and bulk cocoa varieties analyzed by HS-SPME-GC-MS. A total of sixty-four volatile compounds were identified in fresh and dried cocoa. As expected, the volatile profile was clearly modified after the drying step, showing strong differences among cocoa varieties, this factor and its interaction with the drying technique having greater influence according to the ANOVA simultaneous component analysis. A principal component analysis revealed a close relationship between the volatile content of bulk cocoa samples dried by the OD and SD techniques, whereas slight volatile modifications were perceived among fine-flavor samples dried using the three different techniques under study. Overall, the results provide the basis for the potential application of the simplest inexpensive SBPD technique in order to accelerate the drying process of sun drying and produce cocoa with similar (fine-flavor cocoa) or improved (bulk cocoa) aromatic quality to that formed using the traditional SD or the small-scale OD.

The banana is a tropical fruit characterized by its composition of healthy and nutritional compounds. This fruit is part of traditional Ecuadorian gastronomy, being consumed in a wide variety of ways. In this context, unripe Red Dacca banana samples and those submitted to different traditional Ecuadorian heating treatments (boiling, roasting, and baking) were evaluated to profile their phenolic content by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) and the antioxidant activity by ORAC, ABTS, and DPPH assays. A total of sixty-eight phenolic compounds were identified or tentatively identified in raw banana and treated samples, highlighting the content in flavonoids (flavan-3-ols with 88.33% and flavonols with 3.24%) followed by the hydroxybenzoic acid family (5.44%) in raw banana samples. The total phenolic compound content significantly decreased for all the elaborations evaluated, specifically from 442.12 mg/100 g DW in fresh bananas to 338.60 mg/100 g DW in boiled (23.41%), 243.63 mg/100 g DW in roasted (44.90%), and 109.85 mg/100 g DW in baked samples (75.15%). Flavan-3-ols and flavonols were the phenolic groups most affected by the heating treatments, while flavanones and hydroxybenzoic acids showed higher stability against the heating treatments, especially the boiled and roasted samples. In general, the decrease in phenolic compounds corresponded with a decline in antioxidant activity, evaluated by different methods, especially in baked samples. The results obtained from PCA studies confirmed that the impact of heating on the composition of some phenolic compounds was different depending on the technique used. In general, the heating processes applied to the banana samples induced phytochemical modifications. Even so, they remain an important source of bioactive compounds for consumers.

La investigación se llevó a cabo en la Universidad Técnica Estatal de Quevedo. Se valoró la influencia de fermentadores de cacao elaborados de maderas (Guayacán Blanco, Laurel, Pino) sobre la calidad físico-químicas de las almendras de cacao variedad Trinitario y Nacional; análisis sensorial y bromatológica al licor de cacao. Se aplicó un diseño completamente al azar (DCA) con arreglo factorial (3x2), se determinó el porcentaje de fermentaciónen las dos variedades visualizando el 60 % mínimo de almendras bien fermentadas, granos violetas 21 %, granos pizarrosos superó el 12 %, en cuanto al perfil de sabores específicos logró determinarse que el T1 (C. Nacional-C. Laurel) olor a Cacao, T6 (C. Trinitario-Pino) perfil Floral, T2 (C. Trinitario-Guayacán Blanco) Dulce y presenta los mejores atributos; en cuanto al perfil de sabores básicos amargor el T3 (C. Nacional-Pino) tiene promedio alto; el T6 y T5 (Nacional-Guayacán Blanco) obtiene una media en acidez y astringencia; en sabores Verde y Moho el T6 y T3 presentan promedios altos en el licor de cacao. Referente a los análisis físicos químicos, el T6 presenta humedad (2,44%), el T5 (3,37 %) en ceniza, (51,11 %) en grasa el T4 (Trinitario-Laurel), el T6 con un pH de (5,82) y T5 presenta una acidez de (1,34 %) en el licor de cacao. La temperatura máxima alcanzada en la fermentación del cacao Nacional (44,52 °C) en cajones de madera Pino, mientras que la temperatura mínima fue en el cacao Trinitario (35,66 °C) en el fermentador elaborado de madera Guayacán Blanco.

The present work was conducted to evaluate the volatile profile of Ecuadorian Forastero, CCN-51, ETT103 and LR14 cocoa beans during traditional fermentation in laurel wood boxes followed by a sun-drying process. Fifty-six volatiles were identified with HS-SPME-GC–MS. Aldehydes, alcohols and ketones were the compounds that mainly characterized the fresh cocoa. The main compounds formed during the anaerobic fermentation step were esters and acids, while in the aerobic fermentation step, an increase in ester-, aldehyde- and acid-type compounds was observed. Finally, after the drying step, a notable increase in the acid (i.e., acetic acid) content was the predominant trend. According to the genotypes, ETT103 presented high contents of terpenes, alcohols, aldehydes and ketones and low contents of unfavorable acid compounds. The CCN-51 and LR14 (Trinitarian) varieties stood out for their highest amounts in acids (i.e., acetic acid) at the end of primary processing. Finally, the Forastero cocoa beans were highlighted for their low acid and high trimethylpyrazine contents. According to the chemometric and Venn diagram analyses, ETT-103 was an interestingly high-aromatic-quality variety for cocoa gourmet preparations. The results also showed the need for good control of the processing steps (using prefermentative treatments, starter cultures, etc.) on Ecuadorian genotypes of Trinitarian origin.

Pollination is one of the most decisive practices in cocoa cultivation; therefore, to determine the self-compatibility and evaluate the morphology of the flower, it was carried out in the experimental farm "La Represa", Quevedo, Ecuador. For this purpose, a completely randomized DCA design was used, with forty treatments, considering treatments the cocoa hybrids used, with three repetitions. Tukey's multiple range test (P≤0.05) was applied to determine the differences between the means. Manual pollination tests were performed to determine the percentage of fertilization with fifteen flowers per treatment, whose pollination rate stood out in the T26 and T38 treatments with 100% self-compatibility, followed by T15 and T35 with 80% anti-incompatibility. Variables such as the length and width of sepal and petal, peduncle length, length of staminoid and pistil, and the weight of the flower were also recorded where different hybrids stood out, such as T1 and T11 for sepal length and width with 2.70 and 8.20 mm respectively. In the height and width of the petal, the T10 and T26 treatments stood out with 2.50 mm. In the size of the peduncle, I highlight the T20 with 1.81 mm and T6 with 6.20 mm for the length of the staminoid. The length of the pistil, the T10, obtained the highest index at 2.50 mm. However, the weight of the flower highlighted the T13 and T20 treatments, registering the highest average with 0.065 g, concerning the other hybrids studied. Keywords: Autocompatibility, cocoa , hybrids , pollination ; flowers .