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José Cleiton Sousa dos Santos obtained his degree in chemistry at the State University of Ceará‚ (Brazil) in 2008. In 2011, he received his Master’s degree in chemical engineering at the Federal University of Ceará (UFC, Brazil). In 2013, he moved to the ICPCSIC (Spain) under the supervision of Prof. R. Fernandez-Lafuente (ICP-CSIC, Spain), studying new immobilization– stabilization methods for enzymes and in 2015 he obtained his Ph.D. degree (double grade, UAM (Spain)/UFC (Brazil)). In 2015, he received postdoctoral position degree in chemical engineering at the Federal University of Ceará (UFC, Brazil). His research interests are the development of strategies to the preparation of improved biocatalysts: enzyme purification, immobilization, stabilization, and also reaction design. He has coauthored 59 papers in these research áreas, with an h-index of 32 and total citations 2371.
Chitosan is one of the most abundant natural polymer worldwide, and due to its inherent characteristics, its use in industrial processes has been extensively explored. Because it is biodegradable, biocompatible, non-toxic, hydrophilic, cheap, and has good physical-chemical stability, it is seen as an excellent alternative for the replacement of synthetic materials in the search for more sustainable production methodologies. Thus being, a possible biotechnological application of Chitosan is as a direct support for enzyme immobilization. However, its applicability is quite specific, and to overcome this issue, alternative pretreatments are required, such as chemical and physical modifications to its structure, enabling its use in a wider array of applications. This review aims to present the topic in detail, by exploring and discussing methods of employment of Chitosan in enzymatic immobilization processes with various enzymes, presenting its advantages and disadvantages, as well as listing possible chemical modifications and combinations with other compounds for formulating an ideal support for this purpose. First, we will present Chitosan emphasizing its characteristics that allow its use as enzyme support. Furthermore, we will discuss possible physicochemical modifications that can be made to Chitosan, mentioning the improvements obtained in each process. These discussions will enable a comprehensive comparison between, and an informed choice of, the best technologies concerning enzyme immobilization and the application conditions of the biocatalyst.
Yale Luck Nunes; Fernando Lima de Menezes; Isamayra Germano de Sousa; Antônio Luthierre Gama Cavalcante; Francisco Thálysson Tavares Cavalcante; Katerine da Silva Moreira; André Luiz Barros de Oliveira; Gabrielly Ferreira Mota; José Erick Da Silva Souza; Italo Rafael De Aguiar Falcão; Thales Guimaraes Rocha; Roberta Bussons Rodrigues Valério; Pierre Basílio Almeida Fechine; Maria Cristiane Martins de Souza; José C.S. dos Santos. Chemical and physical Chitosan modification for designing enzymatic industrial biocatalysts: How to choose the best strategy? International Journal of Biological Macromolecules 2021, 181, 1124 -1170.
AMA StyleYale Luck Nunes, Fernando Lima de Menezes, Isamayra Germano de Sousa, Antônio Luthierre Gama Cavalcante, Francisco Thálysson Tavares Cavalcante, Katerine da Silva Moreira, André Luiz Barros de Oliveira, Gabrielly Ferreira Mota, José Erick Da Silva Souza, Italo Rafael De Aguiar Falcão, Thales Guimaraes Rocha, Roberta Bussons Rodrigues Valério, Pierre Basílio Almeida Fechine, Maria Cristiane Martins de Souza, José C.S. dos Santos. Chemical and physical Chitosan modification for designing enzymatic industrial biocatalysts: How to choose the best strategy? International Journal of Biological Macromolecules. 2021; 181 ():1124-1170.
Chicago/Turabian StyleYale Luck Nunes; Fernando Lima de Menezes; Isamayra Germano de Sousa; Antônio Luthierre Gama Cavalcante; Francisco Thálysson Tavares Cavalcante; Katerine da Silva Moreira; André Luiz Barros de Oliveira; Gabrielly Ferreira Mota; José Erick Da Silva Souza; Italo Rafael De Aguiar Falcão; Thales Guimaraes Rocha; Roberta Bussons Rodrigues Valério; Pierre Basílio Almeida Fechine; Maria Cristiane Martins de Souza; José C.S. dos Santos. 2021. "Chemical and physical Chitosan modification for designing enzymatic industrial biocatalysts: How to choose the best strategy?" International Journal of Biological Macromolecules 181, no. : 1124-1170.
Among the many biological entities employed in the development of biosensors, enzymes have attracted the most attention. Nanotechnology has been fostering excellent prospects in the development of enzymatic biosensors, since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial in biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, and biocatalytic activity, among others. These and other advantages of nanomaterial-based enzymatic biosensors are discussed in this work via the compilation of several reports on their applications in different industrial segments. To provide detailed insights into the state of the art of this technology, all the relevant concepts around the topic are discussed, including the properties of enzymes, the mechanisms involved in their immobilization, and the application of different enzyme-derived biosensors and nanomaterials. Finally, there is a discussion around the pressing challenges in this technology, which will be useful for guiding the development of future research in the area.
Francisco Cavalcante; Italo de A. Falcão; José Da S. Souza; Thales Rocha; Isamayra de Sousa; Antônio Cavalcante; André de Oliveira; Maria de Sousa; José dos Santos. Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications. Electrochem 2021, 2, 149 -184.
AMA StyleFrancisco Cavalcante, Italo de A. Falcão, José Da S. Souza, Thales Rocha, Isamayra de Sousa, Antônio Cavalcante, André de Oliveira, Maria de Sousa, José dos Santos. Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications. Electrochem. 2021; 2 (1):149-184.
Chicago/Turabian StyleFrancisco Cavalcante; Italo de A. Falcão; José Da S. Souza; Thales Rocha; Isamayra de Sousa; Antônio Cavalcante; André de Oliveira; Maria de Sousa; José dos Santos. 2021. "Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications." Electrochem 2, no. 1: 149-184.
Biofuels have been gaining prominence as alternative technologies to reduce the use of fossil fuels, which are causing major environmental problems such as increasing the greenhouse effect. The design new systems for producing clean, sustainable energy are needed. In this perspective, advances in technologies that increase production of raw materials, reduced costs, and greater efficiency are the great challenge. In this context, nanoparticles are gaining considerable prominence as a platform for designing highly efficient industrial systems. In addition, nanoparticles become quite versatile due to their properties such as small size, high surface area, surface charge, surface chemistry, solubility, and multi-functionality. Here, we review some of the successes with the nanotechnology systems to produce biofuels (as biodiesel, biogas, biohydrogen, bioethanol, algal-derived fuels, jet fuels, and others) together with recent technologies, catalysts and reactors. However, some problems that these nanotechnology systems for biofuels may cause will be discussed, for example production, feedstocks, process design, separation, and purification. Finally, some solutions are proposed, such as types of nanomaterials that have been used in these nanosystems. This chapter gives a comprehensive review of the current challenges and future research directions in the preparation of nanotechnology systems for biofuels production are discussed.
Francisco Thálysson Tavares Cavalcante; Katerine da Silva Moreira; Paula Jéssyca Morais Lima; Rodolpho Ramilton De Castro Monteiro; Bruna Bandeira Pinheiro; Carlos Alberto Chaves Girão Neto; Kimberle Paiva dos Santos; Maria Cristiane Martins de Souza; Rita Karolinny Chaves de Lima; José Cleiton Sousa dos Santos. Nanotechnology Systems for Biofuels Production. Green Organic Reactions 2021, 445 -471.
AMA StyleFrancisco Thálysson Tavares Cavalcante, Katerine da Silva Moreira, Paula Jéssyca Morais Lima, Rodolpho Ramilton De Castro Monteiro, Bruna Bandeira Pinheiro, Carlos Alberto Chaves Girão Neto, Kimberle Paiva dos Santos, Maria Cristiane Martins de Souza, Rita Karolinny Chaves de Lima, José Cleiton Sousa dos Santos. Nanotechnology Systems for Biofuels Production. Green Organic Reactions. 2021; ():445-471.
Chicago/Turabian StyleFrancisco Thálysson Tavares Cavalcante; Katerine da Silva Moreira; Paula Jéssyca Morais Lima; Rodolpho Ramilton De Castro Monteiro; Bruna Bandeira Pinheiro; Carlos Alberto Chaves Girão Neto; Kimberle Paiva dos Santos; Maria Cristiane Martins de Souza; Rita Karolinny Chaves de Lima; José Cleiton Sousa dos Santos. 2021. "Nanotechnology Systems for Biofuels Production." Green Organic Reactions , no. : 445-471.
Chitosan is a natural polysaccharide derived from chitin and extracted from agroindustrial residues such as the exoskeleton of crustaceous and other animals. Considered as one of the most abundant organic materials in nature, it has been widely used in several applications of industrial interest, mainly for its environmentally sustainable properties like biodegradability, biocompatibility, non-toxicity, and renewability. Due to the presence of amino groups in their chemical structure, chitosan has great versatility of modifications and formulations for industrial applications, such as controlled release, surface modification, and preparation of nanoparticles. Here, we review some of the successes with chitosan nanoparticles as biomedical applications and their preparation, ionic cross-linked emulsified chitosan, absorption and bioavailability, delivery systems, quality monitoring, and wastewater treatment. However, some problems that these chitosan nanoparticles may cause will be discussed, for example, mechanical resistance, dissolution, and hydrophilicity/hydrophobicity under certain conditions. Finally, some solutions are proposed, like crosslinking agents, and physicochemical modifications, to manipulate particle size and stability. This chapter gives a comprehensive review of the advantages and recent developments in the formulation of chitosan nanoparticles as an alternative for sustainable agriculture.
André Luiz Barros de Oliveira; Francisco Thálysson Tavares Cavalcante; Katerine da Silva Moreira; Paula Jéssyca Morais Lima; Rodolpho Ramilton De Castro Monteiro; Bruna Bandeira Pinheiro; Kimberle Paiva dos Santos; José Cleiton Sousa dos Santos. Chitosan Nanoparticle: Alternative for Sustainable Agriculture. Green Organic Reactions 2021, 95 -132.
AMA StyleAndré Luiz Barros de Oliveira, Francisco Thálysson Tavares Cavalcante, Katerine da Silva Moreira, Paula Jéssyca Morais Lima, Rodolpho Ramilton De Castro Monteiro, Bruna Bandeira Pinheiro, Kimberle Paiva dos Santos, José Cleiton Sousa dos Santos. Chitosan Nanoparticle: Alternative for Sustainable Agriculture. Green Organic Reactions. 2021; ():95-132.
Chicago/Turabian StyleAndré Luiz Barros de Oliveira; Francisco Thálysson Tavares Cavalcante; Katerine da Silva Moreira; Paula Jéssyca Morais Lima; Rodolpho Ramilton De Castro Monteiro; Bruna Bandeira Pinheiro; Kimberle Paiva dos Santos; José Cleiton Sousa dos Santos. 2021. "Chitosan Nanoparticle: Alternative for Sustainable Agriculture." Green Organic Reactions , no. : 95-132.
Glycerol is a common by‐product of industrial biodiesel syntheses. Due to its properties, availability, and versatility, residual glycerol can be used as raw material in the production of high value‐added industrial inputs and outputs. In particular, products like hydrogen, propylene glycol, acrolein, epichlorohydrin, dioxalane and dioxane, glycerol carbonate, n‐butanol, citric acid, ethanol, butanol, propionic acid, (mono‐, di‐ and triacylglycerols), cynamoil esters, glycerol acetate, benzoic acid, and other applications. In this context, the present study presents a critical evaluation of the innovative technologies based on the use of residual glycerol in different industries, including the pharmaceutical, textile, food, cosmetic, and energy sectors. Chemical and biochemical catalysts in the transformation of residual glycerol are explored, along with the factors to be considered regarding the choice of catalyst route used in the conversion process, aiming at improving the production of these industrial products. This article is protected by copyright. All rights reserved
Paula Jéssyca Morais Lima; Rhonyele Maciel da Silva; Carlos Alberto Chaves Girão Neto; Natan Câmara Gomes e Silva; José Erick Da Silva Souza; Yale Luck Nunes; José Cleiton Sousa dos Santos. An overview on the conversion of glycerol to value‐added industrial products via chemical and biochemical routes. Biotechnology and Applied Biochemistry 2021, 1 .
AMA StylePaula Jéssyca Morais Lima, Rhonyele Maciel da Silva, Carlos Alberto Chaves Girão Neto, Natan Câmara Gomes e Silva, José Erick Da Silva Souza, Yale Luck Nunes, José Cleiton Sousa dos Santos. An overview on the conversion of glycerol to value‐added industrial products via chemical and biochemical routes. Biotechnology and Applied Biochemistry. 2021; ():1.
Chicago/Turabian StylePaula Jéssyca Morais Lima; Rhonyele Maciel da Silva; Carlos Alberto Chaves Girão Neto; Natan Câmara Gomes e Silva; José Erick Da Silva Souza; Yale Luck Nunes; José Cleiton Sousa dos Santos. 2021. "An overview on the conversion of glycerol to value‐added industrial products via chemical and biochemical routes." Biotechnology and Applied Biochemistry , no. : 1.
André L. B. de Oliveira; Francisco T. T. Cavalcante; Katerine S. Moreira; Rodolpho R. C. Monteiro; Thales G. Rocha; José E. S. Souza; Aluísio M. da Fonseca; Ada A. S. Lopes; Artemis P. Guimarães; Rita K. C. de Lima; Maria C. M. de Souza; José C. S. dos Santos. Lipases Immobilized onto Nanomaterials as Biocatalysts in Biodiesel Production: Scientific Context, Challenges, and Opportunities. Revista Virtual de Química 2021, 1 .
AMA StyleAndré L. B. de Oliveira, Francisco T. T. Cavalcante, Katerine S. Moreira, Rodolpho R. C. Monteiro, Thales G. Rocha, José E. S. Souza, Aluísio M. da Fonseca, Ada A. S. Lopes, Artemis P. Guimarães, Rita K. C. de Lima, Maria C. M. de Souza, José C. S. dos Santos. Lipases Immobilized onto Nanomaterials as Biocatalysts in Biodiesel Production: Scientific Context, Challenges, and Opportunities. Revista Virtual de Química. 2021; ():1.
Chicago/Turabian StyleAndré L. B. de Oliveira; Francisco T. T. Cavalcante; Katerine S. Moreira; Rodolpho R. C. Monteiro; Thales G. Rocha; José E. S. Souza; Aluísio M. da Fonseca; Ada A. S. Lopes; Artemis P. Guimarães; Rita K. C. de Lima; Maria C. M. de Souza; José C. S. dos Santos. 2021. "Lipases Immobilized onto Nanomaterials as Biocatalysts in Biodiesel Production: Scientific Context, Challenges, and Opportunities." Revista Virtual de Química , no. : 1.
The rising global demand for sustainable energy resources is resulting in an accelerated increase in biodiesel consumption. In this sense, studies aimed at tackling process hurdles in biodiesel production have been continuously carried out. In order to reduce energy consumption and the amount of wastewater generated, as well as to avoid the production of inefficient end products, classes of enzymes, especially lipases, are being successfully explored as substitutes to chemical catalysts. This article highlights several aspects of lipase-catalyzed biodiesel production. The recent advances and the future perspectives of mechanisms that could circumvent the well-known problems inherent in these systems are presented and discussed, such as the low-stability and the pricing of biocatalysts. According to the literature, alternative solutions include the use of low-cost, unconventional raw materials, new supports, the elucidation of mechanisms of lipase immobilization, and optimal designs and operational settings for bioreactors. Finally, there is a discussion around the necessary steps to enable an economically-viable industrial production.
Francisco Thálysson Tavares Cavalcante; Francisco Simao Neto; Italo Rafael De Aguiar Falcão; José Erick Da Silva Souza; Lourembergue Saraiva De Moura Junior; Patrick Da Silva Sousa; Thales Guimaraes Rocha; Isamayra Germano de Sousa; Pedro Henrique De Lima Gomes; Maria Cristiane Martins de Souza; José C.S. dos Santos. Opportunities for improving biodiesel production via lipase catalysis. Fuel 2020, 288, 119577 .
AMA StyleFrancisco Thálysson Tavares Cavalcante, Francisco Simao Neto, Italo Rafael De Aguiar Falcão, José Erick Da Silva Souza, Lourembergue Saraiva De Moura Junior, Patrick Da Silva Sousa, Thales Guimaraes Rocha, Isamayra Germano de Sousa, Pedro Henrique De Lima Gomes, Maria Cristiane Martins de Souza, José C.S. dos Santos. Opportunities for improving biodiesel production via lipase catalysis. Fuel. 2020; 288 ():119577.
Chicago/Turabian StyleFrancisco Thálysson Tavares Cavalcante; Francisco Simao Neto; Italo Rafael De Aguiar Falcão; José Erick Da Silva Souza; Lourembergue Saraiva De Moura Junior; Patrick Da Silva Sousa; Thales Guimaraes Rocha; Isamayra Germano de Sousa; Pedro Henrique De Lima Gomes; Maria Cristiane Martins de Souza; José C.S. dos Santos. 2020. "Opportunities for improving biodiesel production via lipase catalysis." Fuel 288, no. : 119577.
There are growing demands by the biodiesel companies to get cheap lipase formulation, preferably in liquid forms, which can give an answer to their needs. Thus, many commercial or home-produced enzymes are being used in this process in free form. Among these enzymes, Eversa is a liquid lipase formulation derived from the lipase from Thermomyces lanuginosus designed for the enzymatic biodiesel production. The potential of this promising enzyme is hereby reported. However, the specificity of lipases is a problem in biodiesel production. That way the concept of an optimal lipase for biodiesel production is not appropriate, being preferable the use of concept of combi-lipases, using Eversa as a component of the lipase mixture. The application of Eversa for the production of biodiesel through the transesterification, esterification and hydroesterification of oils and fats with different free fatty acids content is thoroughly discussed. Finally, even though Eversa was launched to be used as liquid biocatalyst in the production of biodiesel, this work will discuss how the immobilization of this enzyme through different strategies enhances its performance. That is, a properly designed immobilized Eversa biocatalyst may be more expensive, but it may have some advantages that overcome this drawback.
Rodolpho R.C. Monteiro; Sara Arana-Peña; Thays N. da Rocha; Letícia P. Miranda; Ángel Berenguer-Murcia; Paulo W. Tardioli; José C.S. dos Santos; Roberto Fernandez-Lafuente. Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution? Renewable Energy 2020, 164, 1566 -1587.
AMA StyleRodolpho R.C. Monteiro, Sara Arana-Peña, Thays N. da Rocha, Letícia P. Miranda, Ángel Berenguer-Murcia, Paulo W. Tardioli, José C.S. dos Santos, Roberto Fernandez-Lafuente. Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution? Renewable Energy. 2020; 164 ():1566-1587.
Chicago/Turabian StyleRodolpho R.C. Monteiro; Sara Arana-Peña; Thays N. da Rocha; Letícia P. Miranda; Ángel Berenguer-Murcia; Paulo W. Tardioli; José C.S. dos Santos; Roberto Fernandez-Lafuente. 2020. "Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?" Renewable Energy 164, no. : 1566-1587.
In this work, the combination of different lipases was employed for the production of biodiesel, using the Free Fatty Acids (FFAs) from Residual Chicken Oil (RCO) as a substrate. As biocatalysts, lipases A and B from Candida antarctica (CALA and CALB, respectively) and lipase from Rhizomucor miehei (RML) were used in different combinations; as a result, the best lipase cocktail was composed of 67% CALB and 33% RML, which was used to optimize the esterification reaction parameters (molar ratio (FFAs/ ethyl alcohol), biocatalyst content, temperature and time) by the Taguchi method. Under optimized conditions (1:5, 15% of lipase cocktail, 30 °C and 3 h), it was possible to achieve 89.95 ± 0.3% of conversion.
Thales G. Rocha; Pedro H. De L. Gomes; Maria C. M. de Souza; Rodolpho R. C. Monteiro; José C. S. dos Santos. Lipase Cocktail for Optimized Biodiesel Production of Free Fatty Acids from Residual Chicken Oil. Catalysis Letters 2020, 151, 1155 -1166.
AMA StyleThales G. Rocha, Pedro H. De L. Gomes, Maria C. M. de Souza, Rodolpho R. C. Monteiro, José C. S. dos Santos. Lipase Cocktail for Optimized Biodiesel Production of Free Fatty Acids from Residual Chicken Oil. Catalysis Letters. 2020; 151 (4):1155-1166.
Chicago/Turabian StyleThales G. Rocha; Pedro H. De L. Gomes; Maria C. M. de Souza; Rodolpho R. C. Monteiro; José C. S. dos Santos. 2020. "Lipase Cocktail for Optimized Biodiesel Production of Free Fatty Acids from Residual Chicken Oil." Catalysis Letters 151, no. 4: 1155-1166.
The immobilization of enzymes using protein coated micro-crystals (PCMCs) was reported for the first time in 2001 by Kreiner and coworkers. The strategy is very simple. First, an enzyme solution must be prepared in a concentrated solution of one compound (salt, sugar, amino acid) very soluble in water and poorly soluble in a water-soluble solvent. Then, the enzyme solution is added dropwise to the water soluble solvent under rapid stirring. The components accompanying the enzyme are called the crystal growing agents, the solvent being the dehydrating agent. This strategy permits the rapid dehydration of the enzyme solution drops, resulting in a crystallization of the crystal formation agent, and the enzyme is deposited on this crystal surface. The reaction medium where these biocatalysts can be used is marked by the solubility of the PCMC components, and usually these biocatalysts may be employed in water soluble organic solvents with a maximum of 20% water. The evolution of these PCMC was to chemically crosslink them and further improve their stabilities. Moreover, the PCMC strategy has been used to coimmobilize enzymes or enzymes and cofactors. The immobilization may permit the use of buffers as crystal growth agents, enabling control of the reaction pH in the enzyme environments. Usually, the PCMC biocatalysts are very stable and more active than other biocatalysts of the same enzyme. However, this simple (at least at laboratory scale) immobilization strategy is underutilized even when the publications using it systematically presented a better performance of them in organic solvents than that of many other immobilized biocatalysts. In fact, many possibilities and studies using this technique are lacking. This review tried to outline the possibilities of this useful immobilization strategy.
Rodolpho Monteiro; José Dos Santos; Andrés Alcántara; Roberto Fernandez-Lafuente. Enzyme-Coated Micro-Crystals: An Almost Forgotten but Very Simple and Elegant Immobilization Strategy. Catalysts 2020, 10, 891 .
AMA StyleRodolpho Monteiro, José Dos Santos, Andrés Alcántara, Roberto Fernandez-Lafuente. Enzyme-Coated Micro-Crystals: An Almost Forgotten but Very Simple and Elegant Immobilization Strategy. Catalysts. 2020; 10 (8):891.
Chicago/Turabian StyleRodolpho Monteiro; José Dos Santos; Andrés Alcántara; Roberto Fernandez-Lafuente. 2020. "Enzyme-Coated Micro-Crystals: An Almost Forgotten but Very Simple and Elegant Immobilization Strategy." Catalysts 10, no. 8: 891.
In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol–ethylenediamine–glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic strength and in absence or presence of 0.5% (v/v) Triton X-100). The biocatalysts were characterized for thermal and organic solvents stabilities and compared with the soluble enzyme. The biocatalysts were then applied to the hydrolysis of the rac-indanyl acetate (2:1 ratio enzyme/substrate) at pH 7.0 and 30 °C for 24 h. For all the strategies evaluated, GEG promoted kinetic resolution of rac-indanyl acetate with maximum conversion (50%) and led to (R)-indanol with excellent enantiomeric excess (97%), maintaining the maximum conversion for five consecutive cycles of hydrolysis. Therefore, the use of cashew apple bagasse has proved to be a promising eco-friendly support for enzyme immobilization, since it resulted in stable biocatalysts for enzymatic kinetic resolution.
Ticiane C. De Souza; Thiago De Sousa Fonseca; Jouciane De Sousa Silva; Paula J. M. Lima; Carlos A. C. G. Neto; Rodolpho R. C. Monteiro; Maria Valderez P. Rocha; Marcos C. De Mattos; José C. S. Dos Santos; Luciana R. B. Gonçalves. Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate. Bioprocess and Biosystems Engineering 2020, 43, 2253 -2268.
AMA StyleTiciane C. De Souza, Thiago De Sousa Fonseca, Jouciane De Sousa Silva, Paula J. M. Lima, Carlos A. C. G. Neto, Rodolpho R. C. Monteiro, Maria Valderez P. Rocha, Marcos C. De Mattos, José C. S. Dos Santos, Luciana R. B. Gonçalves. Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate. Bioprocess and Biosystems Engineering. 2020; 43 (12):2253-2268.
Chicago/Turabian StyleTiciane C. De Souza; Thiago De Sousa Fonseca; Jouciane De Sousa Silva; Paula J. M. Lima; Carlos A. C. G. Neto; Rodolpho R. C. Monteiro; Maria Valderez P. Rocha; Marcos C. De Mattos; José C. S. Dos Santos; Luciana R. B. Gonçalves. 2020. "Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate." Bioprocess and Biosystems Engineering 43, no. 12: 2253-2268.
In this work, the concept of lipase cocktail has been proposed in the ultrasound-assisted hydrolysis of coconut oil. Lipase from Thermomyces lanuginosus (TLL), lipase from Rhizomucor miehei (RML), and lipase B from Candida antarctica (CALB) were evaluated as biocatalysts in different combinations. The best conversion (33.66%) was achieved using only RML; however, the best lipase cocktail (75% RML and 25% CALB) proposed by the triangular response surface was used to achieve higher conversions. At the best lipase cocktail, reaction parameters [temperature, biocatalyst content and molar ratio (water/oil)] were optimized by a Central Composite Design, allowing to obtain more than 98% of conversion in the hydrolysis of coconut oil in 3 h of incubation at 37 kHz, 300 W and 45 °C by using 20% of the lipase cocktail (w/w) and a molar ratio of 7.5:1 (water/oil). The lipase cocktail retained about 50% of its initial activity after three consecutive cycles of hydrolysis. To the authors’ knowledge, up to date, this communication is the first report in the literature for the ultrasound-assisted hydrolysis of coconut oil catalyzed by a cocktail of lipases. Under ultrasound irradiation, the concept of lipase cocktail was successfully applied, and this strategy could be useful for the other types of reactions using heterogeneous substrates.
José E. S. Souza; Rodolpho R. C. Monteiro; Thales G. Rocha; Katerine S. Moreira; Francisco T. T. Cavalcante; Ana K. De Sousa Braz; Maria C. M. de Souza; José C. S. dos Santos. Sonohydrolysis using an enzymatic cocktail in the preparation of free fatty acid. 3 Biotech 2020, 10, 1 -10.
AMA StyleJosé E. S. Souza, Rodolpho R. C. Monteiro, Thales G. Rocha, Katerine S. Moreira, Francisco T. T. Cavalcante, Ana K. De Sousa Braz, Maria C. M. de Souza, José C. S. dos Santos. Sonohydrolysis using an enzymatic cocktail in the preparation of free fatty acid. 3 Biotech. 2020; 10 (6):1-10.
Chicago/Turabian StyleJosé E. S. Souza; Rodolpho R. C. Monteiro; Thales G. Rocha; Katerine S. Moreira; Francisco T. T. Cavalcante; Ana K. De Sousa Braz; Maria C. M. de Souza; José C. S. dos Santos. 2020. "Sonohydrolysis using an enzymatic cocktail in the preparation of free fatty acid." 3 Biotech 10, no. 6: 1-10.
Lipase from Thermomyces lanuginosus (TLL) was immobilized onto a novel heterofunctional support, divinyl sulfone (DVS) superparamagnetic nanoparticles (SPMNs) functionalized with polyethyleneimine (PEI). Particle size and zeta potential measurements, elemental analysis, X-ray powder diffraction, magnetic measurements, and infrared spectroscopy analysis were used to characterize the TLL preparations. At pH 10, it was possible to achieve 100 % of immobilization yield in 1 h. The immobilization pH gives TLL preparations with different stabilities; indeed the TLL preparation immobilized at pH 5.0 was the most stable during the thermal inactivation at all pH values. For the hydrolysis of racemic methyl mandelate, the nanobiocatalysts immobilized at pH 5.0 and blocked with ethylenediamine (EDA) and ethanolamine (ETA) obtained good enantioselectivities (68 % and 72 %, respectively) with high catalytic activities in the reaction medium at pH 7.0. The operational stability of the systems was evaluated in the esterification reaction of benzyl alcohol, obtaining up to 61 % conversion after the seventh reaction cycle. These results show that [email protected] support is a robust strategy for the easy and rapid recovery of the nanobiocatalyst by applying a magnetic field, showing great potential for industrial applications.
Rayanne M. Bezerra; Rodolpho R. C. Monteiro; Davino Machado Andrade Neto; Francisco F.M. da Silva; Regina de Paula; Telma L.G. de Lemos; Pierre B.A. Fechine; Marcio Correa; Felipe Bohn; Luciana R.B. Gonçalves; José C.S. dos Santos. A new heterofunctional support for enzyme immobilization: PEI functionalized Fe3O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus. Enzyme and Microbial Technology 2020, 138, 109560 .
AMA StyleRayanne M. Bezerra, Rodolpho R. C. Monteiro, Davino Machado Andrade Neto, Francisco F.M. da Silva, Regina de Paula, Telma L.G. de Lemos, Pierre B.A. Fechine, Marcio Correa, Felipe Bohn, Luciana R.B. Gonçalves, José C.S. dos Santos. A new heterofunctional support for enzyme immobilization: PEI functionalized Fe3O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus. Enzyme and Microbial Technology. 2020; 138 ():109560.
Chicago/Turabian StyleRayanne M. Bezerra; Rodolpho R. C. Monteiro; Davino Machado Andrade Neto; Francisco F.M. da Silva; Regina de Paula; Telma L.G. de Lemos; Pierre B.A. Fechine; Marcio Correa; Felipe Bohn; Luciana R.B. Gonçalves; José C.S. dos Santos. 2020. "A new heterofunctional support for enzyme immobilization: PEI functionalized Fe3O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus." Enzyme and Microbial Technology 138, no. : 109560.
Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) and a central composite design (CCD) were used to optimize the esterification and study the effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature (30–50 °C), biocatalyst content (0.05–0.15 g) and reaction time (2–6 h)) in the conversion into fatty acid ethyl esters. Under optimized conditions (1:18 molar ratio (FFAs/alcohol), 0.14 g of Novozym® 435, 48 °C and 4 h), the conversion into ethyl esters was 96.8%. It was found that after 10 consecutive cycles of esterification under optimal conditions, Novozym® 435 showed a maximum loss of activity of 5.8%, suggesting a very small change in the support/enzyme ratio proved by Fourier Transform Infrared (FTIR) spectroscopy and insignificant changes in the surface of Novozym® 435 proved by scanning electron microscopy (SEM) after the 10 consecutive cycles of esterification.
Katerine S. Moreira; Lourembergue S. Moura Júnior; Rodolpho R. C. Monteiro; André L. B. De Oliveira; Camila P. Valle; Tiago Melo Freire; Pierre B. A. Fechine; Maria C. M. De Souza; Gloria Fernandez-Lorente; José M. Guisan; José C. S. Dos Santos. Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM. Catalysts 2020, 10, 414 .
AMA StyleKaterine S. Moreira, Lourembergue S. Moura Júnior, Rodolpho R. C. Monteiro, André L. B. De Oliveira, Camila P. Valle, Tiago Melo Freire, Pierre B. A. Fechine, Maria C. M. De Souza, Gloria Fernandez-Lorente, José M. Guisan, José C. S. Dos Santos. Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM. Catalysts. 2020; 10 (4):414.
Chicago/Turabian StyleKaterine S. Moreira; Lourembergue S. Moura Júnior; Rodolpho R. C. Monteiro; André L. B. De Oliveira; Camila P. Valle; Tiago Melo Freire; Pierre B. A. Fechine; Maria C. M. De Souza; Gloria Fernandez-Lorente; José M. Guisan; José C. S. Dos Santos. 2020. "Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM." Catalysts 10, no. 4: 414.
This review intends to present some of the latest studies on the lipase A from Candida antarctica (CALA). This lipase is among the most stable ones and has some capability to attack the sn-2 position of triglycerides. This makes it a very interesting lipase, especially considering that it is commercially available. The cloning and production of the enzyme together with some structural facts and applications will be discussed in this review. Special focus will be put on the immobilization of the enzyme. The use of the commercially available crosslinked enzyme aggregates of this enzyme will be explained, together with the use of the enzyme in some new trends in enzyme immobilization, such as bio-imprinting of the open form of CALA by detergents and the fixation of the open structure, the design of heterofunctional supports able to take full advantage of the immobilization via interfacial activation but preventing enzyme release, or the design of strategies for the preparation of multiple layers of lipase enzymes (using just CALA or combining CALA with other lipases).
Rodolpho R.C. Monteiro; Jose Juan Virgen-Ortiz; Ángel Berenguer-Murcia; Thays N. da Rocha; José C.S. dos Santos; Andrés R. Alcántara; Roberto Fernandez-Lafuente. Biotechnological relevance of the lipase A from Candida antarctica. Catalysis Today 2020, 362, 141 -154.
AMA StyleRodolpho R.C. Monteiro, Jose Juan Virgen-Ortiz, Ángel Berenguer-Murcia, Thays N. da Rocha, José C.S. dos Santos, Andrés R. Alcántara, Roberto Fernandez-Lafuente. Biotechnological relevance of the lipase A from Candida antarctica. Catalysis Today. 2020; 362 ():141-154.
Chicago/Turabian StyleRodolpho R.C. Monteiro; Jose Juan Virgen-Ortiz; Ángel Berenguer-Murcia; Thays N. da Rocha; José C.S. dos Santos; Andrés R. Alcántara; Roberto Fernandez-Lafuente. 2020. "Biotechnological relevance of the lipase A from Candida antarctica." Catalysis Today 362, no. : 141-154.
The emission of gases from the fleet of vehicles is one of the main sources of the environmental impact of the planet. Reversing this scenario, environmental awareness and a search for alternative sources of energy has been growing exponentially in recent years. In this context, the production of biofuels presents itself as an alternative for the lowest environmental impact, energy, and economy, with emphasis on biodiesel. The scale of production of this biofuel, the options related to the raw materials and their conditions are intertwined with the energy and exergetic efficiencies of the process. Therefore, no work was constructed as an analysis of the biodiesel production processes of soybeans and babassu, in which they were conducted on a laboratory scale. According to the results, the production of soybean biodiesel arose in exergetic efficiency of 73.25% and the production of biodiesel from babassu of 74.72%.
Antônio Alison Da Silva Mamede; Ada Amélia Sanders Lopes; Rosali Barbosa Marques; Jackson Queiroz Malveira; Maria Alexsandra De Sousa Rios. Soybean and babassu biodiesel production: a laboratory scale study and an exergy analysis approach. Matéria (Rio de Janeiro) 2020, 25, 1 .
AMA StyleAntônio Alison Da Silva Mamede, Ada Amélia Sanders Lopes, Rosali Barbosa Marques, Jackson Queiroz Malveira, Maria Alexsandra De Sousa Rios. Soybean and babassu biodiesel production: a laboratory scale study and an exergy analysis approach. Matéria (Rio de Janeiro). 2020; 25 (4):1.
Chicago/Turabian StyleAntônio Alison Da Silva Mamede; Ada Amélia Sanders Lopes; Rosali Barbosa Marques; Jackson Queiroz Malveira; Maria Alexsandra De Sousa Rios. 2020. "Soybean and babassu biodiesel production: a laboratory scale study and an exergy analysis approach." Matéria (Rio de Janeiro) 25, no. 4: 1.
Elizabete Carneiro; Ana Bastos; Ulisses De Oliveira; Leonardo De Matos; Wellington Adriano; Rodolpho Monteiro; José Dos Santos; Luciana Gonçalves. IMPROVING THE CATALYTIC FEATURES OF THE LIPASE FROM Rhizomucor miehei IMMOBILIZED ON CHITOSAN-BASED HYBRID MATRICES BY ALTERING THE CHEMICAL ACTIVATION CONDITIONS. Química Nova 2020, 1 .
AMA StyleElizabete Carneiro, Ana Bastos, Ulisses De Oliveira, Leonardo De Matos, Wellington Adriano, Rodolpho Monteiro, José Dos Santos, Luciana Gonçalves. IMPROVING THE CATALYTIC FEATURES OF THE LIPASE FROM Rhizomucor miehei IMMOBILIZED ON CHITOSAN-BASED HYBRID MATRICES BY ALTERING THE CHEMICAL ACTIVATION CONDITIONS. Química Nova. 2020; ():1.
Chicago/Turabian StyleElizabete Carneiro; Ana Bastos; Ulisses De Oliveira; Leonardo De Matos; Wellington Adriano; Rodolpho Monteiro; José Dos Santos; Luciana Gonçalves. 2020. "IMPROVING THE CATALYTIC FEATURES OF THE LIPASE FROM Rhizomucor miehei IMMOBILIZED ON CHITOSAN-BASED HYBRID MATRICES BY ALTERING THE CHEMICAL ACTIVATION CONDITIONS." Química Nova , no. : 1.
The use of biological methods for industrial manufacturing is an excellent starting point to create a green process since biocatalysts naturally follows the Principles of Green Chemistry. Biocatalysts are highly biodegradable and are considered natural products. Nevertheless, they also have some drawbacks, since enzyme have high molecular complexity, high production costs and intrinsic fragility. Several strategies have been developed in order to stabilize enzymes under process conditions: chemical modification, enzyme immobilization, aggregation, modern techniques of protein engineering and so on. Enzyme improvement via immobilization is a useful technique of enzyme engineering, which allows not only the re-use of enzymes but also the enhancement of enzyme properties. Some reasons to engineer enzymes include better accommodate unnatural substrates, increase their stability under the reaction conditions and create new reactions or new biochemical pathways. Among enzymes, lipases appear as important tools in the application of the principles of Green Chemistry, offering a convenient way to prepare derivatives of natural compounds with great potential in food and pharmaceutical industries. With this in mind, some strategies to improve enzyme performance at industrial scale and ways of conducting processes will be discussed.
Bruna B. Pinheiro; Kímberle P. Dos Santos; Nathalia S. Rios; André Casimiro De Macedo; José C.S. Dos Santos; Luciana R.B. Gonçalves. Enzymatic Reactions and Biocatalytic Processes. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering 2019, 1 .
AMA StyleBruna B. Pinheiro, Kímberle P. Dos Santos, Nathalia S. Rios, André Casimiro De Macedo, José C.S. Dos Santos, Luciana R.B. Gonçalves. Enzymatic Reactions and Biocatalytic Processes. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. 2019; ():1.
Chicago/Turabian StyleBruna B. Pinheiro; Kímberle P. Dos Santos; Nathalia S. Rios; André Casimiro De Macedo; José C.S. Dos Santos; Luciana R.B. Gonçalves. 2019. "Enzymatic Reactions and Biocatalytic Processes." Reference Module in Chemistry, Molecular Sciences and Chemical Engineering , no. : 1.
The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters.
Rodolpho R. C. Monteiro; Davino M. Andrade Neto; Pierre B. A. Fechine; Ada A. S. Lopes; Luciana R. B. Gonçalves; José C. S. Dos Santos; Maria C. M. De Souza; Roberto Fernandez-Lafuente. Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation. International Journal of Molecular Sciences 2019, 20, 5807 .
AMA StyleRodolpho R. C. Monteiro, Davino M. Andrade Neto, Pierre B. A. Fechine, Ada A. S. Lopes, Luciana R. B. Gonçalves, José C. S. Dos Santos, Maria C. M. De Souza, Roberto Fernandez-Lafuente. Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation. International Journal of Molecular Sciences. 2019; 20 (22):5807.
Chicago/Turabian StyleRodolpho R. C. Monteiro; Davino M. Andrade Neto; Pierre B. A. Fechine; Ada A. S. Lopes; Luciana R. B. Gonçalves; José C. S. Dos Santos; Maria C. M. De Souza; Roberto Fernandez-Lafuente. 2019. "Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation." International Journal of Molecular Sciences 20, no. 22: 5807.
Coconut (Cocos nucifera L.) juice has been immobilized onto hydrogel microcapsules, and its performance in the bio-acylation of quinine has been evaluated. Under optimized conditions, the biocatalyst has been used for acetylation reactions with several advantages. The protein encapsulation percentages obtained were 98.1%, with an unimodal distribution with a medium size of around 760 μm. The morphology of lyophilized microcapsules was observed by Scanning Electronic Microscopy (SEM), which partially retained their structure. The catalyst microcapsules were reactive with acetylation reactions and by some alcohols.
Aluísio Marques Da Fonseca; José Cleiton Sousa Dos Santos; Maria Cristiane Martins De Souza; Mauro Macedo de Oliveira; Regilany Paulo Colares; Telma Leda Gomes De Lemos; Raimundo Braz Filho. The use of new hydrogel microcapsules in coconut juice as biocatalyst system for the reaction of quinine. Industrial Crops and Products 2019, 145, 111890 .
AMA StyleAluísio Marques Da Fonseca, José Cleiton Sousa Dos Santos, Maria Cristiane Martins De Souza, Mauro Macedo de Oliveira, Regilany Paulo Colares, Telma Leda Gomes De Lemos, Raimundo Braz Filho. The use of new hydrogel microcapsules in coconut juice as biocatalyst system for the reaction of quinine. Industrial Crops and Products. 2019; 145 ():111890.
Chicago/Turabian StyleAluísio Marques Da Fonseca; José Cleiton Sousa Dos Santos; Maria Cristiane Martins De Souza; Mauro Macedo de Oliveira; Regilany Paulo Colares; Telma Leda Gomes De Lemos; Raimundo Braz Filho. 2019. "The use of new hydrogel microcapsules in coconut juice as biocatalyst system for the reaction of quinine." Industrial Crops and Products 145, no. : 111890.