Soil degradation has been reported worldwide. To better understand this degradation, we selected Pinus armandii and Quercus aliena var. acuteserrata forests, and a mixed forest of Q. aliena var. acuteserrata and P. armandii in the Qinling Mountains in China for our permanent plots and conducted three investigations over a 20-year period. We determined the amounts of available nitrogen (N) and phosphorus (P) in the soil to track the trajectory of soil quality and compared these with stand characteristics, topographic and climatic attributes to analyze the strength of each factor in influencing the available N and P in the soil. We found that the soil experienced a severe drop in quality, and that degradation is continuing. Temperature is the most critical factor controlling the soil available N, and species composition is the main factor regulating the soil available P. Given the huge gap in content and the increasing rate of nutrients loss, this reduction in soil quality will likely negatively affect ecosystem sustainability.

The transcription factor WRKY is widely distributed in the plant kingdom, playing a significant role in plant growth, development and response to stresses. Walnut is an economically important temperate tree species valued for both its edible nuts and high-quality wood, and its response to various stresses is an important factor that determines the quality of its fruit. However, in walnut trees themselves, information about the WRKY gene family remains scarce. In this paper, we perform a comprehensive study of the WRKY gene family in walnut. In total, we identified 103 WRKY genes in the common walnut that are clustered into 4 groups and distributed on 14 chromosomes. The conserved domains all contained a WRKY domain, and motif 2 was observed in most WRKYs, suggesting a high degree of conservation and similar functions within each subfamily. However, gene structure was significantly differentiated between different subfamilies. Synteny analysis indicates that there were 56 gene pairs in J. regia and A. thaliana, 76 in J. regia and J. mandshurica, 75 in J. regia and J. microcarpa, 76 in J. regia and P. trichocarpa, and 33 in J. regia and Q. robur, indicating that the WRKY gene family may come from a common ancestor. GO and KEGG enrichment analysis showed that the WRKY gene family was involved in resistance traits and the plant-pathogen interaction pathway. In anthracnose-resistant F26 fruits (AR) and anthracnose-susceptible F423 fruits (AS), transcriptome and qPCR analysis results showed that JrWRKY83, JrWRKY73 and JrWRKY74 were expressed significantly more highly in resistant cultivars, indicating that these three genes may be important contributors to stress resistance in walnut trees. Furthermore, we investigate how these three genes potentially target miRNAs and interact with proteins. JrWRKY73 was target by the miR156 family, including 12 miRNAs; this miRNA family targets WRKY genes to enhance plant defense. JrWRKY73 also interacted with the resistance gene AtMPK6, showing that it may play a crucial role in walnut defense.

The rapid urbanization process means that even moderate-sized cities can quickly become part of larger urban agglomerations, creating new urban zones. Urban Green Infrastructure (UGI) plays a crucial role in these clusters, acting as precious green spaces essential for maintaining ecological safety. This study combines fluctuation analysis based on Morphological Spatial Pattern with traditional landscape pattern analysis, comprehensively addressing the evolution of UGI in terms of quantity, characteristics, and morphology. We selected the Taiyuan-Jinzhong agglomeration as our study area, which is currently in an agglomeration process. The results demonstrated the critical role of surrounding mountains as natural ecological barrier zones. During urban agglomeration, management strategies focused on large-scale afforestation to ensure the quantity of UGI. However, this approach also led to a more clustered landscape with reduced connectivity. Additionally, linear or small-scale UGI types such as branch and islet have seen reductions over the past decade. Changes in internal morphological and complex fluctuations within UGI can harm the formation of ecological networks and potentially negatively affect biodiversity and ecological safety. The research highlights how ecological protection and urban planning policies can influence UGI fluctuations. Therefore, urban managers should not just concentrate on maintaining the quantity of UGI, but also give consideration to changes in its internal features and morphology. Before cities further agglomerate into larger urban clusters, it is crucial to address deficiencies in UGI, continuously improving type configurations and functional structures at the landscape scale. Through strategic planning of UGI, cities can mitigate ecological risks and foster sustainable urban development.

Fatty acid desaturase (FAD) is a core catalytic enzyme in plants for the synthesis of unsaturated fatty acids, profoundly affecting plant growth, development, and adaptability to various environmental stresses. The walnut (Juglans regia L.) is an important woody oil tree species, and its kernel is rich in unsaturated fatty acids. Systematic identification of the walnut FAD gene family and analysis of its function are of great significance for revealing the molecular mechanisms underlying unsaturated fatty acid metabolism in the walnut. Based on walnut whole-genome data, this study used homology alignment and hidden Markov model search methods to identify the JrFAD gene family members. Subsequently, a variety of bioinformatics tools were used to systematically analyze their structural characteristics, evolutionary expansion mechanism, expression regulation, and function. A total of 21 JrFAD gene family members were identified and classified into five subfamilies. The family genes were unevenly distributed on nine chromosomes. WGD/segmental duplication was the main expansion method, and the duplicated gene pairs experienced strong purification selection. The family gene promoter sequence is rich in regulatory elements that respond to light, plant hormones, and various stresses. The expression pattern analysis showed that JrFAD3.1 and JrFAD2.3 showed high expression specifically during the rapid accumulation of walnut kernel oil. This study clarified the composition and evolutionary characteristics of the FAD gene family in the walnut, which provides useful information for in-depth analyses of its functional mechanism in the regulation of lipid metabolism, and also identified potential candidate gene resources for the genetic improvement of walnut varieties with high amounts of unsaturated fatty acids.

Leaf C:N:P stoichiometry provides a functional link between plant nutrient composition and environmental adaptation. However, whether widely distributed tree species maintain consistent leaf stoichiometric patterns across contrasting vegetation zones remains unclear. Here, we investigated the leaf C:N:P stoichiometry of Quercus variabilis, a widely distributed tree species in China, across the subtropical evergreen broad-leaved forest zone (SEF) and the warm-temperate deciduous broad-leaved forest zone (WDF). We aimed to clarify between-zone differences in leaf stoichiometric traits and the role of climate and soil constraints in shaping vegetation-zone-dependent stoichiometric variation. Our results showed that leaf C:N:P stoichiometry of Q. variabilis differed between the SEF and WDF, with significant differences detected only for leaf N and leaf C:N. Overall, leaf C varied little across zones (CV = 3.13–3.42%), leaf N and leaf C:N showed moderate variation (CV = 10.07–13.26%), whereas leaf P, leaf C:P, and leaf N:P showed greater variability (CV = 33.28–39.93%). Environmental analyses further indicated contrasting patterns between zones: leaf stoichiometry in the SEF was jointly associated with climate and soil factors and showed a positive leaf C–N relationship, whereas that in the WDF showed a stronger soil association and a negative leaf N–P relationship. These findings suggest that Q. variabilis may adjust its leaf stoichiometry in a zone-dependent manner in response to contrasting climate and soil conditions. This study provides a theoretical basis for understanding nutrient-use strategies and environmental adaptation of widely distributed tree species under heterogeneous climate and soil conditions.