Host-virus associations have co-evolved under ecological and evolutionary selection pressures that shape cross-species transmission and spillover to humans. Observed virus-host associations provide relevant context for newly discovered wildlife viruses to assess knowledge gaps in host-range and estimate pathways for potential human infection. Using models to predict virus-host networks, we predicted the likelihood of humans as hosts for 513 newly discovered viruses detected by large-scale wildlife surveillance at high-risk animal-human interfaces in Africa, Asia, and Latin America. Predictions indicated that novel coronaviruses are likely to infect a greater number of host species than viruses from other families. Our models further characterize novel viruses through prioritization scores and directly inform surveillance targets to identify host ranges for newly discovered viruses.

Species of the genus Dipetalonema are parasitic nematodes of the family Onchocercidae (Nematoda; Filarioidea) which infect the peritoneal cavity of Neotropical primates. Of these, six species have been taxonomically described, two of these have been reported infecting the black-faced spider monkey (Ateles chamek): Dipetalonema gracile and Dipetalonema yatesi. Description of Dipetalonema species have been based on morphological characteristics, and their phylogenetic relationships remain unresolved. A few molecular studies have been carried out in Dipetalonema spp. infecting Neotropical primates. Seven filarioid nematodes (6 females and one male) recovered from one A. chamek in the Peruvian Amazon rainforest were morphologically identified as D. yatesi and molecularly characterized. A multi-locus genetic analysis of nuclear ribosomal region (18S) and mitochondrial (cox1, 12S, and nad5) gene sequences supported D. yatesi as a distinct lineage and yielded a highly resolved phylogenetic lineage tree for this filarioid genus of Neotropical primates. Our results highlighted that Dipetalonema species are divided in two well-supported clades, one containing D. yatesi and D. caudispina, and the second containing D. robini, D. gracile, and D. graciliformis. Due to sequence ambiguities from GenBank entries, relationships among isolates of D. gracile and D. graciliformis cannot be fully resolved, which requires further investigation. However, this suggests that these could represent a species complex. Our study confirms that D. yatesi is a valid species and constitutes the first molecular phylogenetic analysis of this parasite in black-faced spider monkeys.

Peru has 55 primate taxa (including all species and subspecies), a third of which are threatened. The major drivers of habitat loss, degradation and fragmentation are grazing, forestry, agriculture and transport infrastructure. Other activities such as hunting exacerbate these threats. We assessed the threats from degradation and fragmentation facing Peruvian primates to aid in the design and implementation of mitigation strategies. Through GIS-based mapping, statistical modeling and specialist assessments, we evaluated all primate taxa using the IUCN Conservation Measures Partnership Unified Classifications of Direct Threats across five categories (direct threats to primates, threats to habitat, causes of fragmentation, factors exacerbating fragmentation and threats to primates and habitats as a consequence of fragmentation), highlighting which were most common and most severe. Our results showed that all primate taxa were affected by degradation and fragmentation in Peru. The most common and severe direct threat was hunting, whereas housing and urban development, smallholder crop farming, smallholder grazing and large-scale logging were the most common and severe threats across the other categories. The families Cebidae and Atelidae face the highest overall threat. Our analysis showed that the current IUCN listing of Leontocebus leucogenys [LC] underestimates the true threat level this species faces and that Lagothrix lagothricha tschudii [DD] should be listed under one of the threat categories. In Peru, the need for mitigating the threat of habitat fragmentation is clear. To ensure the survival of Peru’s diverse primate taxa, forest connectivity needs to be maintained or recovered through the protection and restoration of key areas considering their biological and social needs.

Wildlife trafficking creates favorable scenarios for intra- and inter-specific interactions that can lead to parasite spread and disease emergence. Among the fauna affected by this activity, primates are relevant due to their potential to acquire and share zoonoses - infections caused by parasites that can spread between humans and other animals. Though it is known that most primate parasites can affect multiple hosts and that many are zoonotic, comparative studies across different contexts for animal-human interactions are scarce. We conducted a multi-parasite screening targeting the detection of zoonotic infections in wild-caught monkeys in nine Peruvian cities across three contexts: captivity (zoos and rescue centers, n = 187); pet (households, n = 69); and trade (trafficked or recently confiscated, n = 132). We detected 32 parasite taxa including mycobacteria, simian foamyvirus, bacteria, helminths, and protozoa. Monkeys in the trade context had the highest prevalence of hemoparasites (including Plasmodium malariae/brasilianum, Trypanosoma cruzi, and microfilaria) and enteric helminths and protozoa were less common in pet monkeys. However, parasite communities showed overall low variation between the three contexts. Parasite richness (PR) was best explained by host genus and the city where the animal was sampled. Squirrel (genus Saimiri) and wooly (genus Lagothrix) monkeys had the highest PR, which was ~2.2 times the PR found in tufted capuchins (genus Sapajus) and tamarins (genus Saguinus/Leontocebus) in a multivariable model adjusted for context, sex, and age. Our findings illustrate that the threats of wildlife trafficking to One Health encompass exposure to multiple zoonotic parasites well-known to cause disease in humans, monkeys, and other species. We demonstrate these threats continue beyond the markets where wildlife is initially sold; monkeys trafficked for the pet market remain a reservoir for and contribute to the translocation of zoonotic parasites to households and other captive facilities where contact with humans is frequent. Our results have practical applications for the healthcare of rescued monkeys and call for urgent action against wildlife trafficking and ownership of monkeys as pets.

Wildlife trafficking and human–wildlife conflict are major causes of species decline. The illegal nature of wildlife trafficking makes it hard, and sometimes dangerous, to study. ‘Mansión Mascota’ is a veterinary clinic in Guayaquil, Ecuador, which, through agreement with the Ecuadorian Ministry of the Environment, receives confiscated wildlife for treatment. Mansión Mascota also receives injured and abandoned wildlife brought in by the authorities and the public. Between January 2018 and September 2022, the clinic received 3212 wild animals from ≥171 taxa, including mammals, birds, reptiles, and amphibians. Through the classification of records based on reported use, native distribution, and evidence of capture/pet keeping, we were able to classify 1127 animals of 68 species as subject to wildlife trafficking. Turtles were the most abundant group (69%). The majority of turtles (91%) and primates (80%) were of Amazonian origin, whereas 90% of psittacines and 97% of carnivores had potential coastal origins. Threatened and conservation-dependent species were common in the traffic. Furthermore, ≥955 animals of 106 species were brought to the clinic due to anthropogenic wildlife conflict. Trafficking, combined with the high number of injured animals, highlights the synergistic threats facing wildlife in Ecuador.