Background: The face is the area most exposed to the normal course of skin aging, both intrinsically and extrinsically. The aim of the study was to evaluate the cellular and clinical response of a therapeutic protocol aimed at countering facial skin aging. Materials and Methods: Twenty female patients with facial skin laxity and photodamage underwent combined therapy including mesotherapy using non-cross-linked hyaluronic acid with calcium hydroxyapatite and an infrared energy-based device treatment with subsequent implementation of PEG-cross-linked hyaluronic acid soft tissue fillers. To evaluate the benefits, patients underwent histological, immunological, and biomechanical evaluations before the treatment and at 21 and 150 days after the treatment. Results: The histological results at 21 days and 150 days after the procedure showed an increase in the number of fibroblasts and angiogenesis. As for the immunological aspect, it was shown that the treatment has an immunomodulating action, avoiding the activation of CD4 and CD8 cells. Biomechanical data showed that, at 150 days after treatment, the average changes in skin elasticity increased by 72% and the skin hydration increased by 49%. Conclusions: A combination of an infrared energy-based device treatment with both non-cross-linked hyaluronic acid and novel PEG-cross-linked hyaluronic acid leads to numerous positive cutaneous changes after histological, immunological, and biomechanical evaluations.

The method of suicide attempt is related to motivational processes and the psycho(patho)logical mechanisms and traits of an individual. Indirect self-destructiveness is related to direct self-destructiveness. It is presumed that it can transform to the latter thus leading to suicide attempts or death by suicide. The study objective was to examine the relationship between individual manifestations of indirect self-destructiveness and the methods of suicide attempt as well as to explore the indirect predictors of particular suicide methods. The study was conducted among 147 persons (114 females, 33 males) who attempted suicide. The research instrument was the Polish version of the “Chronic Self-Destructiveness Scale” (CS-DS), including Transgression and Risk, Poor Health Maintenance, Personal and Social Neglects, Lack of Planfulness, and Helplessness and Passiveness in the face of problems. Correlation and regression analyses were applied. A number of statistically significant correlations were found between indirect self-destructiveness, or its manifestations, and the methods of suicide attempt. Moreover, the particular categories of indirect self-destructive behaviour were found to largely determine the choice of the method of suicide attempt. Among these categories, the strongest predictor appeared to be Helplessness and Passiveness in the face of problems. The method of suicide attempt is a variable related to psychosocial determinants of suicidal behaviour. The findings of this study may prove useful in the design and implementation of therapeutic activities focused on persons who attempted suicide. Recognising the particular manifestations of indirect self-destructive behaviours of an attempter can guide implementation of therapeutic measures, for him/her e.g. via strengthening coping skills and eliminating risk factors for self-harm.

The aim of this study was to test the effect of hyaluronic acid cross-linked with polyethylene glycol containing micronized portions of calcium hydroxyapatite (Neauvia Stimulate) on both local tissue and systemic consequences, which are crucial from the perspective of long-term safety, in patients suffering from Hashimoto’s disease. This most common autoimmune disease is a frequently mentioned contraindication to the use of fillers based on hyaluronic acid as well as biostimulants based on calcium hydroxyapatite. Broad-spectrum aspects of histopathology were analyzed to identify key features of inflammatory infiltration before the procedure and 5, 21, and 150 days after the procedure. A statistically significant effect on the reduction of the intensity of the inflammatory infiltration in the tissue in relation to the state before the procedure was demonstrated, combined with a reduction in the occurrence of both antigen-recognizing (CD4) and cytotoxic (CD8) T lymphocytes. With complete statistical certainty, it was demonstrated that the treatment with Neauvia Stimulate had no effect on the levels of these antibodies. All this corresponds with the risk analysis that showed no alarming symptoms during the time of observation. The choice of hyaluronic acid fillers cross-linked with polyethylene glycol should be considered justified and safe in the case of patients suffering from Hashimoto’s disease.

Purpose: This prospective, single-center, open-label study aimed to evaluate the efficacy and safety of 1470 nm non-ablative laser therapy in treating facial acne scars. The primary objective was to assess improvements in skin texture, elasticity, and scar depth and diameter, while confirming the absence of significant adverse effects. Patients and Methods: 40 healthy female volunteers aged 18 to 42 years with facial acne scars underwent three sessions of 1470 nm laser therapy at two-week intervals. Outcome measures included cutometric assessments, high-frequency ultrasound evaluations, and clinical photographic documentation, conducted at baseline and on days 21, 42, 70, and 130 post-treatment. Results: Significant improvements were observed across all evaluated parameters. Skin elasticity increased progressively from baseline (mean: 62.95, SD=4.0) to day 21 (63.72, SD=3.8, +4%), day 42 (67.11, SD=4.0, +6%), day 70 (69.08, SD=4.2, +11%), and day 130 (70.80, SD=4.4, +14%; p< 0.001). High-frequency ultrasound measurements revealed substantial reductions in scar depth, which decreased by 5% at day 21 (mean: 0.18 mm, SD=0.05, p< 0.001), 20% at day 42 (0.15 mm, SD=0.055, p< 0.001), 48% at day 70 (0.10 mm, SD=0.06, p< 0.001), and 63% at day 130 (0.07 mm, SD=0.065, p< 0.001). Scar diameter followed a similar trend, with reductions of 16% at day 21 (3.08 mm, SD=0.3, p< 0.001), 26% at day 42 (2.71 mm, SD=0.34, p< 0.001), 42% at day 70 (2.13 mm, SD=0.36, p< 0.001), and 62% at day 130 (1.39 mm, SD=0.38, p< 0.001). Clinical photographic evaluations corroborated these quantitative findings, showing visible improvements in scar appearance and overall skin texture. No adverse events were reported throughout the study. Conclusion: The 1470 nm non-ablative laser therapy demonstrated high efficacy and safety in the treatment of acne scars, delivering substantial therapeutic benefits and high patient satisfaction, offering a valuable therapeutic option for addressing both pigmentation and textural issues associated with acne scars.

Objective: This study aimed to evaluate the reconstitution and solubility characteristics of two injectable poly L-lactic acid (PLLA) formulations, the next-generation injectable PLLA-LASYNPRO™ and a previous-generation PLLA (PLLA-SCA), through in vitro analysis. Methods: The study, conducted from 20 November to 11 December 2024, involved reconstituting PLLA-LASYNPRO™ in 5 mL saline solution and PLLA-SCA in 8 mL sterile water or saline (as specified in the instructions for use). Reconstitution processes were recorded using a 4K camera, and microscopic and sieve-based analyses were performed at various time points (1–120 min). The study used standardized methods for reconstitution, observation, and sample collection, including a light microscope and micrometric sieves. Results: PLLA-LASYNPRO™ exhibited rapid, uniform reconstitution, resulting in a homogeneous milky-white solution with no foam formation. In contrast, PLLA-SCA showed a two-phase mixture with foam formation, requiring more vigorous shaking. Over time, PLLA-LASYNPRO™ displayed gradual sedimentation, with no foam, while PLLA-SCA showed a progressive increase in foam volume and sedimentation. Microscopic analysis revealed consistent particle morphology, with PLLA-LASYNPRO™ particles being smaller and homogeneous, and PLLA-SCA exhibiting larger, irregular particles prone to aggregation. Sieve analysis showed that PLLA-LASYNPRO™ produced minimal sediment, whereas PLLA-SCA consistently produced sediment on both 71 µm and 200 µm sieves. Conclusions: PLLA-LASYNPRO™ and PLLA-SCA display distinct reconstitution behaviors, with PLLA-LASYNPRO™ showing more consistent characteristics and fewer issues with foam and sedimentation. These differences may impact clinical applications and require consideration in treatment planning.

Objectives: To evaluate the cellular and clinical responses to facial treatment using a wide-spectrum, long-pulse infrared medical device. Methods: Thirty patients with facial skin laxity and photodamage underwent a single treatment session with the infrared device. Skin biopsies were collected before treatment and on days 5 and 21 for histological and immunohistochemical assessments of inflammatory and regenerative changes. Long-term outcomes were analyzed by biomechanical measurements (cutometry) and collagen autofluorescence quantification from histological samples. Results: Histological analysis demonstrated a 40% increase in fibroblast count, angiogenesis, and mild inflammatory activity, though these changes did not reach statistical significance. Cutometric evaluation revealed a significant improvement in skin elasticity at day 21 (+15.97%) and day 150 (+24.49%) post-treatment, accompanied by a modest increase in hydration. Collagen autofluorescence showed a statistically significant enhancement, consistent with ECM remodeling and fibroblast activation. Conclusions: A single session of treatment with a wide-spectrum, long-pulse infrared device produced measurable improvements in dermal elasticity and collagen organization with minimal inflammatory response. These findings support the efficacy and safety of infrared energy-based therapy as a noninvasive modality for facial skin rejuvenation.