Radiation transform models such as PROSAIL are widely used for crop canopy reflectance simulation and biophysical parameter inversion. The PROSAIL model basically assumes that the canopy is turbid homogenous media with a bare soil background. However, the canopy structure changes when crop growth stages develop, which is more or less a departure from this assumption. In addition, a paddy rice field is inundated most of the time with flooded soil background. In this study, field-scale paddy rice leaf area index (LAI), leaf cholorphyll content (LCC), and canopy chlorophyll content (CCC) were retrieved from unmanned-aerial-vehicle-based hyperspectral images by the PROSAIL radiation transform model using a lookup table (LUT) strategy, with a special focus on the effects of growth-stage development and soil-background signature selection. Results show that involving flooded soil reflectance as background reflectance for PROSAIL could improve estimation accuracy. When using a LUT with the flooded soil reflectance signature ($LU{T}_{flooded}$) the coefficients of determination ($R^2$) between observed and estimation variables are 0.70, 0.11, and 0.79 for LAI, LCC, and CCC, respectively, for the entire growing season (from tillering to heading growth stages), and the corresponding mean absolute errors ($MAEs$) are 21.87%, 16.27%, and 12.52%. For LAI and LCC, high model bias mainly occurred in tillering growth stages. There is an obvious overestimation of LAI and underestimation of LCC for in the tillering growth stage. The estimation accuracy of CCC is relatively consistent from tillering to heading growth stages.

Flooding intensity significantly alters the availability of iron (Fe), zinc (Zn), and cadmium (Cd) in paddy soil. However, the influence of arbuscular mycorrhizal fungi (AMF) on the uptake and transfer of Cd and micronutrients (Fe and Zn) under Cd stress in varying flooding conditions is not well understood. A pot experiment was conducted to investigate the micronutrient homeostasis and Cd uptake and transfer in rice cultivated in Cd-contaminated soil with AMF inoculation under continuous and intermittent flooding conditions. Compared to non-inoculation controls, mycorrhizal inoculation decreased Cd concentration in rice plants under continuous and intermittent flooding, and improved grain yield by 39.2 % for early season rice and 21.1 % for late season rice under continuous flooding. Mycorrhizal inoculation balanced the availability of Zn and Fe and decreased the availability of Cd in soil, lowering the ratios of soil-available Cd to both soil-available Zn and soil Fe²⁺. These changes led to a redistribution of Zn and Fe concentrations in rice, thereby reducing Cd acquisition in a soil-rice system. Structural equation model (SEM) analysis revealed that mycorrhizal inoculation had a strong direct negative effect on the expression of Zn and Fe-related genes OsNRAMP1, OsIRT1, and OsIRT2 in the roots of rice, which in turn directly affected root Cd concentration. Furthermore, mycorrhizal colonization decreased Cd transfer coefficients from leaves to grains under continuous flooding and from nodes and leaves to grains under intermittent flooding. In the nodes, the Fe concentration and the expression of genes OsIRT1 and OsHMA2 were associated with Cd transfer from the nodes to grains. Similarly, in the leaves, the expression of genes OsZIP1 and OsMTP1 corresponded with Cd transfer from leaves to grains. This study provides insights into the role of AMF in affecting micronutrient concentrations and Cd uptake in rice under varying flooding conditions.

Flooding is a critical factor that limits the establishment of a symbiosis between rice and arbuscular mycorrhizal fungi (AMF) in wetland ecosystems. The distribution of carbon resources in roots and the acclimation strategies of rice to flooding stress in the presence of AMF are poorly understood. We conducted a root box experiment, employing nylon sheets or nylon meshes to create separate fungal chambers that either prevented or allowed the roots and any molecules to pass through. We found that the mycorrhizal colonization rate and the expression of genes OsD14L and OsCERK1, which are involved in fungal perception during symbiosis, both increased in mycorrhizal rice roots following intermittent flooding compared to continuous flooding. Furthermore, AMF inoculation affected root morphological traits, facilitating both shallower and deeper soil exploration. Increased submergence intensity led to carbohydrate deprivation in roots, while high mycorrhizal colonization increased soil oxygen consumption and decreased the neutral lipid concentration in roots. However, mycorrhizal inoculation increased the rice photosynthesis rate and facilitated acclimation to submergence by mediating the expression of the genes OsCIPK15 and OsSUB1A to enhance rice shoot elongation and the sugar concentration in roots as a result of reduced competition for carbon between rice and AMF under different flooding conditions.