The growth and productivity of maize are severely affected by soil salinity. The crucial determinants for the future performance of plants are productive for seed germination and seedling establishment; however, both stages are liable to soil salinity. For grain, maize is an economically significant crop sensitive to abiotic stresses. However, little is known about defense responses by the salinity-induced antioxidant and oxidative stress in maize. In our work, the commercially available maize variety Raka-Poshi was grown in pots for 30 days under greenhouse conditions. To evaluate the salt-induced oxidative/antioxidant responses in maize for salt stress 0, 25, 50, 75, 100 and 150 mM concentrations, treatments were provided using sodium chloride (NaCl). All the biochemical indices were calculated under all NaCl concentrations, while drought was induced by up to 50% irrigation water. After 30 days of seed germination, the maize leaves were collected for the measurement of lipid peroxidase or malondialdehyde (MDA), glutathione reductase (GR), guaiacol peroxidase (GPOD), hydrogen peroxide (H₂O₂), superoxide dismutase (SOD), lipoxygenase (LOX), catalase (CAT), ascorbate peroxidase (APOD) and glutathione-S-transferase (GST). The results revealed a 47% reduction under 150 mM NaCl and 50% drought stress conditions. The results have shown that the successive increase of NaCl concentrations and drought caused an increase in catalase production. With successive increase in NaCl concentration and drought stress, lower levels of H₂O₂, SOD, and MDA were detected in maize leaves. The results regarding the morphology of maize seedlings indicated a successive reduction in the root length and shoot length under applications of salt and drought stress, while root-to-shoot weights were found to be increased under drought stress and decreased under salt stress conditions During gene expression analysis collectively indicate that, under drought stress conditions, the expression levels of all nine mentioned enzyme-related genes were consistently downregulated.

YABBY is among the specific transcription factor (TF) gene family in plants and plays an important role in the development of the leaves and floral organs. Its specific roles include lateral organ development, the establishment of dorsoventral polarity, and response to abiotic stress. Potato is an important crop worldwide and YABBY genes are not still identified and characterized in potato. So, little has been known about YABBY genes in potato until now. This study was carried out to perform genome-wide analysis, which will provide an in-depth analysis about the role of YABBY genes in potato. There have been seven StYAB genes identified, which are found to be located on seven different chromosomes. Through multiple sequence analyses, it has been predicted that the YABBY domain was present in all seven genes while the C2-C2 domain was found to be absent only in StYAB2. With the help of cis-element analysis, the involvement of StYAB genes in light, stress developmental, and hormonal responsiveness has been found. Furthermore, expression analysis from RNA-seq data of different potato organs indicated that all StYAB genes have a role in the vegetative growth of the potato plant. In addition to this, RNA-seq data also identified StYAB3, StYAB5, and StYAB7 genes showing expression during cadmium, and drought stress, while StYAB6 was highly expressed during a viral attack. Moreover, during the attack of Phytophthora infestans on a potato plant StYAB3, StYAB5, StYAB6, and StYAB7 showed high expression. This study provides significant knowledge about the StYAB gene structures and functions, which can later be used for gene cloning, and functional analysis; this information may be utilized by molecular biologists and plant breeders for the development of new potato lines.

Rice (Oryza sativa L.) is a staple food for more than 50% of the world’s population. Rice cultivar improvement is critical in order to feed the world’s growing population. Improving yield is one of the main aims of rice breeders. However, yield is a complex quantitative trait controlled by many genes. The presence of genetic diversity is the key factor to improve the yield hence, the presence of diversity in any germplasm is important for yield improvement. In the current study, the rice germplasm was collected from Pakistan and the United States of America and a panel of 100 diverse genotypes was utilized to identify important yield and yield-related traits. For this, a genome-wide association study (GWAS) was performed to identify the genetic loci related to yield. The GWAS on the diverse germplasm will lead to the identification of new genes which can be utilized in the breeding program for improvement of yield. For this reason, firstly, the germplasm was phenotypically evaluated in two growing seasons for yield and yield-related traits. The analysis of variance results showed significant differences among traits which showed the presence of diversity in the current germplasm. Secondly, the germplasm was also genotypically evaluated using 10K SNP. Genetic structure analysis showed the presence of four groups which showed that enough genetic diversity was present in the rice germplasm to be used for association mapping analysis. The results of GWAS identified 201 significant marker trait associations (MTAs. 16 MTAs were identified for plant height, 49 for days to flowering, three for days to maturity, four for tillers per plant, four for panicle length, eight for grains per panicle, 20 unfilled grains per panicle, 81 for seed setting %, four for thousand-grain weight, five for yield per plot and seven for yield per hectare. Apart from this, some pleiotropic loci were also identified. The results showed that panicle length (PL) and thousand-grain weight (TGW) were controlled by a pleiotropic locus OsGRb23906 on chromosome 1 at 10,116,371 cM. The loci OsGRb25803 and OsGRb15974 on chromosomes 4 and 8 at the position of 14,321,111 cM and 6,205,816 cM respectively, showed pleiotropic effects for seed setting % (SS) and unfilled grain per panicle (UG/P). A locus OsGRb09180 on chromosome 4 at 19,850,601 cM was significantly linked with SS and yield/ha. Furthermore, gene annotation was performed, and results indicated that the 190 candidate genes or QTLs that closely linked with studied traits. These candidate genes and novel significant markers could be useful in marker-assisted gene selection and QTL pyramiding to improve rice yield and the selection of potential parents, recombinants and MTAs which could be used in rice breeding programs to develop high-yielding rice varieties for sustainable food security.

The emergence of drug-resistant bacteria poses a substantial threat to global healthcare, necessitating the development of novel therapeutic approaches. Seriphidium kurramense, a well-known aromatic, medicinal, and culinary herb, has garnered attention due to its potential medicinal properties. In this study, we investigated the chemical composition, antibacterial, and antibiofilm activities of Seriphidium kurramense plant extract. The coupled analysis of GC/MS (Gas chromatography/ mass spectrometry) has identified seventeen components in the extract, with major constituents including α-santonin (42.2%), eucalyptol (3.04%), and bicyclo[3.1.0]hexan-3-one (10.1%). The research encompassed four bacterial strains (E. coli, Bacillus, Staphylococcus aureus, and Salmonella), all involved in biofilm formation. The results indicated that the plant extract exhibited varying efficacy against the tested bacterial strains. Additionally, the ADME profiling of the identified phytocompounds supported their potential as drug candidates with promising pharmacokinetic properties. In-silico molecular docking studies revealed that among the 17 identified phytocompounds, lumisantonin displayed the highest effectiveness as an antibiofilm and antibacterial agent. It was closely followed by n-Hexadecanoic acid, ß-Copaene, and other compounds examined in the docking studies. These compounds exhibited high binding scores with target proteins, suggesting their potential as therapeutic agents. This comprehensive investigation not only sheds light on the chemical composition and pharmacological properties of Seriphidium kurramense compound but also presents a promising avenue for developing innovative drugs against drug-resistant bacterial strains. The results of this investigation provide valuable insights to the ongoing efforts in addressing the pressing issue of drug-resistant bacteria in global healthcare.

The consumption of tomatoes has been associated with diminishing the risk of several lethal diseases, e.g., heart attack and cancer. This is because tomato contains high antioxidants that have been shown to protect against oxidative damage in numerous empirical and epidemiological studies. Considering the health benefits, more emphasis should be given to produce organic tomatoes. Tomatoes have been ranked as the most important fruit and vegetable in Western diets as essential source of antioxidants such as lycopene, β-carotene, phenols, vitamin E, and vitamin C. Environmental conditions and agricultural practices are key factors that affect the quantities of these compounds available in tomato. Therefore, controlling the environmental conditions, such as water availability, temperature, light, saline soil, and agricultural practices (fertilization practices, harvesting, and food storage) are valuable tools to enhance the nutritional value of tomato fruits organically. Although, the quantitative and qualitative contents of health-promoting compounds in vegetables and fruits depend on their genetic predispositions. Agricultural practices and different environmental condition have broad effects on the nutraceutical compounds. Thus, this present study emphasizes on enhancing tomato nutrition through improved agricultural practices and optimized farming, especially in saline and water-deficit conditions. This organic-oriented strategy may counteract the scepticism caused by genetically modified tomatoes (GMOs) and will prompt further exploration in future studies.Keywords: Organic grown tomato; Antioxidants; Nutritional values; Salinity; Water deficit; Temperature; Agricultural practices; Fertilization