Due to modernization and urbanization, traditional medicinal herbs and their associated knoweldge are under peril of being lost. The current study not only aimed to document the trend of herbal medicinal plant uitlization in District Lahore, Punjab, Pakistan, but also raised alarming concerns about the conservation of these medicinal herbs and traditional knowledge linked to them. The data were collected from local people, herbalists, and gardeners by using standard ethnobotanical methods, i.e., questionnaires and interviews. Results reported that a lower number of informants possessed medicinal herb knowledge and reported only 50 medicinal herbs from the study area. This is probably due to the impact of speedy industrilaization and alterations in human life patterns in the study area. Not only the traditional knowledge is under threat but also the medicinal herbs themselves. Most of the reported medicinal herbs were rare (45.10%), while only few (9.80%) were ranked as abundant in the study area. The herbs with more medicinal importance are mostly rare. This is because of their over usage by the local people. Both conservation approaches (in situ and ex situ conservation and cultivation practices) and resource management (good agricultural practices and sustainable use solutions) should be adequately taken into account for the sustainable use of medicinal plant resources.

Rice (Oryza sativa L.) is a staple food for more than 50% of the world’s population. Rice cultivar improvement is critical in order to feed the world’s growing population. Improving yield is one of the main aims of rice breeders. However, yield is a complex quantitative trait controlled by many genes. The presence of genetic diversity is the key factor to improve the yield hence, the presence of diversity in any germplasm is important for yield improvement. In the current study, the rice germplasm was collected from Pakistan and the United States of America and a panel of 100 diverse genotypes was utilized to identify important yield and yield-related traits. For this, a genome-wide association study (GWAS) was performed to identify the genetic loci related to yield. The GWAS on the diverse germplasm will lead to the identification of new genes which can be utilized in the breeding program for improvement of yield. For this reason, firstly, the germplasm was phenotypically evaluated in two growing seasons for yield and yield-related traits. The analysis of variance results showed significant differences among traits which showed the presence of diversity in the current germplasm. Secondly, the germplasm was also genotypically evaluated using 10K SNP. Genetic structure analysis showed the presence of four groups which showed that enough genetic diversity was present in the rice germplasm to be used for association mapping analysis. The results of GWAS identified 201 significant marker trait associations (MTAs. 16 MTAs were identified for plant height, 49 for days to flowering, three for days to maturity, four for tillers per plant, four for panicle length, eight for grains per panicle, 20 unfilled grains per panicle, 81 for seed setting %, four for thousand-grain weight, five for yield per plot and seven for yield per hectare. Apart from this, some pleiotropic loci were also identified. The results showed that panicle length (PL) and thousand-grain weight (TGW) were controlled by a pleiotropic locus OsGRb23906 on chromosome 1 at 10,116,371 cM. The loci OsGRb25803 and OsGRb15974 on chromosomes 4 and 8 at the position of 14,321,111 cM and 6,205,816 cM respectively, showed pleiotropic effects for seed setting % (SS) and unfilled grain per panicle (UG/P). A locus OsGRb09180 on chromosome 4 at 19,850,601 cM was significantly linked with SS and yield/ha. Furthermore, gene annotation was performed, and results indicated that the 190 candidate genes or QTLs that closely linked with studied traits. These candidate genes and novel significant markers could be useful in marker-assisted gene selection and QTL pyramiding to improve rice yield and the selection of potential parents, recombinants and MTAs which could be used in rice breeding programs to develop high-yielding rice varieties for sustainable food security.

Okra a verdant vegetable, is a member of the Malvacae family. Many insect pests attack okra crop but jassid (Amrasca biguttula biguttula) is one of the most devastating sucking pests causing significant losses. Laboratory and field investigations were carried out to find out the efficacy of biopesticides against the jassid, on okra crop at the Faculty of Agricultural Sciences, University of Punjab, Lahore. A. Esculentus seeds were sown in a randomized complete block design with four replications in March 2023. Treatments applied were T0 (control), T1 Citrullus colocynthisis extract), T2 (Datura stramonium stramonium extract), T3 (Eucalyptus globulus extract), T4 (Ferula assafoetida extract), T5 (Nicotiana tabacum extract), and T6 (Detergent) as the least hazardous chemical for comparison. A 20% v/v concentration of each treatment was applied as the pest population peaked. Pre-treatment observations were made 24 hours before the application of biopesticides, while post-treatment observations were made at 24, 48, and 72-hour intervals, as well as after one week and ten days. The results showed that all the bio-pesticides reduced the jassid population by up to one-week intervals under laboratory and field conditions. Under field conditions, the N. tabacum extracts reduced the per leaf population up to 78%) followed by F. assafoetida (77%), C. colocynthisis (63%), E. globulus (51%), D. stramonium (42%) and Detergent (25%). In the contrary, no significant differences were observed among N. tabacum extract, F. assafoetida, and C. colocynthisis extracts after 72 hours. It is concluded that bio-pesticides derived from N. tabacum, F. assafoetida, and C. colocynthis are highly effective to combat the jassid infestation.

Honglian type cytoplasmic male sterility (CMS) is one of the three known major CMS types of rice (Oryza sativa L.) commercially used in hybrid rice seed production. Hybrid rice generated by the Honglian type CMS is a special group of hybrid rice, having distinct agricultural characteristics. The main objective of the study was to screen out the Honglian hybrid rice adapted for growing in Pakistan based on desirable traits. Different Honglian-type hybrid rice varietieswere tested locally in different locations in Pakistan based on various desirabletraits. Three Honglian types of hybrids (HP1, HP2, HP3) performed well, had better agricultural traits and showed high yield potential over the check variety. Different qualitative and quantitative traits were studied to conclude the advantages of these varieties for Pakistani local adaptation evaluations. Forty-eight SSR markers were used to study the genetic diversities of the hybrids. Nine selected polymorphic SSR markers (RM-219, RM-236, RM-274, RM-253, RM-424, RM-567, RM-258, RM-481, RM-493) showed genetic variations among Honglian hybrid rice varieties through PCR analysis. In 2019 and 2020, the increment of the yield potential of HP1, HP2 and HP3 was better (+43.90%, +35.44%, +37.13% and +30.91%, +33.37%, +33.62%, respectively, in both years)than the check variety KSK-133. All the desirable traits were analyzed through Principal Component Analysis (PCA). The principal components with more than one eigenvalue showed more variability. The average variability of 74.78% was observed among genotypes and their desirable traits in both years. National Uniform Yield Trial (NUYT) and Distinctness, Uniformity, Stability (DUS) trials are being conducted under the supervision of National Coordinated Rice (NCR) and Federal Seed Certification and Registration Department (FSCRD), Government of Pakistan. In the 2020 trial, the average yield of 104 rice varieties/hybrids was 8608 kg/ha; HP1, HP2 and HP3 (8709 kg/ha, 8833 kg/ha, and 9338 kg/ha, respectively) were all higher than the average yield, and HP3 yield was higher than over check varieties (D-121, Guard-53). In the 2021 trial, the average yield of 137 varieties was 7616 kg/ha; the HP1 yield (7863 kg/ha) was higher than the average overcheck varieties/hybrids. Various qualitative and quantitative traits showed desirable genetic diversity among the rice hybrids. It was also observed that, under higher temperatures, the seeds setting rate of Honglian-type hybrid rice was stable, which is the guarantee for stable yield and rice production in Pakistan. Moreover, it was considerably better, suggesting that Honglian-type hybrid rice varieties can be grown in Pakistan because they are less risky under climate change, especially the global warming challenges.

Oryza sativa L. is a staple diet for billions of people in the world. Different rice varieties' drought stress responses and their effects on both qualitative and quantitative variables. Rice is the second main exportable product of Pakistan and the agriculture sector has shown remarkable growth, with the crop sector posting a growth of 6.58% in Fiscal year 2022. Drought have a bad effect on the vegetative and reproductive stages of crops. Drought stress can reduce crop yield production. The RCBD design, two-way ANOVA factorial analysis, Tukey test, Genetic advance and Heritability were used for this experiment. This study specifically focuses on the response of rice genotypes to drought stress and its effect on morphological parameters such as plant height, panicle length, stem diameter, flag leaf length and width, number of panicles per plant, and 1000-grain weight. Drought tolerance genotypes were screened out and used to the standard Index of IRRI to identify morphological processing in Rice Research Institute Kala Shah Kaku, Pakistan. Total 35 rice genotypes were sown under drought stress with checked variety. Six varieties perform better production under drought stress conditions. These varieties like Pokkoli, Vehari, Nonabokra, Kalomonk, PK10683, and Basmati 375 show better perform under drought stress. The findings of this research can contribute to the development of drought-tolerant rice varieties and enhance food security in drought-prone areas. The importance of qualitative parameters, such as panicle curvature and awning, is also explored, with potential implications for the visual appearance and market preferences of rice grains. Overall, this research provides valuable insights into the genetic basis and potential breeding strategies for improving drought tolerance in rice.

Dengue fever, caused by the Aedes mosquito-borne dengue virus, is still a major public health concern in many parts of the world, including Lahore, Pakistan. Lahore region is regarded as a dengue fever hotspot due to the large number of cases reported each year. The purpose of this research is to give an in-depth analysis of dengue mosquito dynamics in Lahore, with a particular emphasis on determining the influence of dengue epidemics and environmental factors. The current study was based on survey comprising 240 individuals from Lahore. In the study findings, 82.08% were positive cases and 17.91% were negative cases. The majority of respondents, were aware of the symptoms of dengue infection (62.33%), its transmission (90.83%), the cause of dengue (61%), safety precautions (73.33%), and the organ infected by the dengue virus (66.25). However, the majority of people are still clueless. 70% of participants indicate that television is their primary information source. Dengue fever cases have been found to have a seasonal pattern, peaking during the monsoon season (45.41%) when mosquito breeding conditions are perfect. The study's findings revealed that Aedes mosquitos, particularly Aedes aegypti, were widespread and identified as the primary vectors (61.66%) of dengue transmission in Lahore. It was revealed that temperature (49.58%), and rainfall all affected the number of Aedes mosquitoes. Overall, 81.6% were satisfied with Governmental efforts against the spread of dengue fever. Improved public knowledge is necessary to reduce dengue virus incidence. The findings of this study can assist public health professionals and legislators in developing targeted measures to mitigate the consequences of dengue epidemics and protect the public from this terrible infection.