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Antibiotics are considered the cornerstone of modern medicine; however, currently, antibiotic resistance has become a global health issue. Antibiotics also find new uses in the treatment of other pathologies as well as cancer. The present study aimed to verify the impact of tetracycline and ampicillin in a colorectal adenocarcinoma cell line, HT-29. The effects of the two antibiotics on cell viability and nucleus were evaluated by the means of MTT assay and the Hoechst staining method, respectively. The irritant potential at vascular level of the chorioallantoic membrane was tested by the HET-CAM assay. Treatment of HT-29 cells with the two antibiotics determined different effects: (i) tetracycline induced a dose- and time-dependent cytotoxic effect characterized by decreased cell viability, changes in cells morphology, apoptotic features (nuclear fragmentation), and inhibition of cellular migration, whereas (ii) ampicillin exerted a biphasic response—cytotoxic at low doses and proliferative at high concentrations. In terms of effect on blood vessels, both antibiotics exerted a mild irritant effect. These results are promising and could be considered as starting point for further in vitro studies to define the molecular mechanisms involved in the cytotoxic/proliferative effects.
Emil-Florin Hut; Matilda Radulescu; Nicolae Pilut; Ioana Macasoi; Delia Berceanu; Dorina Coricovac; Iulia Pinzaru; Octavian Cretu; Cristina Dehelean. Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity. Current Oncology 2021, 28, 2466 -2480.
AMA StyleEmil-Florin Hut, Matilda Radulescu, Nicolae Pilut, Ioana Macasoi, Delia Berceanu, Dorina Coricovac, Iulia Pinzaru, Octavian Cretu, Cristina Dehelean. Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity. Current Oncology. 2021; 28 (4):2466-2480.
Chicago/Turabian StyleEmil-Florin Hut; Matilda Radulescu; Nicolae Pilut; Ioana Macasoi; Delia Berceanu; Dorina Coricovac; Iulia Pinzaru; Octavian Cretu; Cristina Dehelean. 2021. "Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity." Current Oncology 28, no. 4: 2466-2480.
Melissa officinalis is a medicinal herb with an extensive pharmacological profile that has been proven to have beneficial effects in oral and gastrointestinal disorders. However, the effects of this plant in oral, pharyngeal, and colorectal malignancies, types of cancer with an increased incidence in recent years, are less investigated. The present study aims to evaluate the pharmacological profile of a Melissa officinalis total extract for potential benefits in oral, pharynx and colorectal carcinoma. The LC-MS profile of MO total extract (MOte) indicated a rich content in polyphenols, data that support the potent antioxidant capacity exhibited and the antimicrobial activity against both Gram-negative and Gram-positive bacteria. In addition, MOte triggered a dose-dependent and selective decrease in the viability of tumor cells (tongue and pharynx squamous cell carcinomas, and colorectal adenocarcinoma), with the most significant effect being recorded at 100 μg/mL. At the same concentration, MOte exhibited an antiangiogenic effect by inhibiting the process of angiogenesis in ovo. Overall, our findings support the potential benefits of Melissa officinalis leaf total extract as a valuable candidate for the prophylaxis of oral, pharyngeal and colorectal neoplasms.
Kristine Guran; Roxana Buzatu; Iulia Pinzaru; Madalina Boruga; Iasmina Marcovici; Dorina Coricovac; Stefana Avram; Marioara Poenaru; Monica Susan; Razvan Susan; Daniela Radu; Cristina Dehelean. In Vitro Pharmaco-Toxicological Characterization of Melissa officinalis Total Extract Using Oral, Pharynx and Colorectal Carcinoma Cell Lines. Processes 2021, 9, 850 .
AMA StyleKristine Guran, Roxana Buzatu, Iulia Pinzaru, Madalina Boruga, Iasmina Marcovici, Dorina Coricovac, Stefana Avram, Marioara Poenaru, Monica Susan, Razvan Susan, Daniela Radu, Cristina Dehelean. In Vitro Pharmaco-Toxicological Characterization of Melissa officinalis Total Extract Using Oral, Pharynx and Colorectal Carcinoma Cell Lines. Processes. 2021; 9 (5):850.
Chicago/Turabian StyleKristine Guran; Roxana Buzatu; Iulia Pinzaru; Madalina Boruga; Iasmina Marcovici; Dorina Coricovac; Stefana Avram; Marioara Poenaru; Monica Susan; Razvan Susan; Daniela Radu; Cristina Dehelean. 2021. "In Vitro Pharmaco-Toxicological Characterization of Melissa officinalis Total Extract Using Oral, Pharynx and Colorectal Carcinoma Cell Lines." Processes 9, no. 5: 850.
Melanoma represents one of the most aggressive and drug resistant skin cancers with poor prognosis in its advanced stages. Despite the increasing number of targeted therapies, novel approaches are needed to counteract both therapeutic resistance and the side effects of classic therapy. Betulinic acid (BA) is a bioactive phytocompound that has been reported to induce apoptosis in several types of cancers including melanomas; however, its effects on mitochondrial bioenergetics are less investigated. The present study performed in A375 human melanoma cells was aimed to characterize the effects of BA on mitochondrial bioenergetics and cellular behavior. BA demonstrated a dose-dependent inhibitory effect in both mitochondrial respiration and glycolysis in A375 melanoma cells and at sub-toxic concentrations (10 μM) induced mitochondrial dysfunction by eliciting a decrease in the mitochondrial membrane potential and changes in mitochondria morphology and localization. In addition, BA triggered a dose-dependent cytotoxic effect characterized by apoptotic features: morphological alterations (nuclear fragmentation, apoptotic bodies) and the upregulation of pro-apoptotic markers mRNA expression (Bax, Bad and Bak). BA represents a viable therapeutic option via a complex modulatory effect on mitochondrial metabolism that might be useful in advanced melanoma or as reliable strategy to counteract resistance to standard therapy.
Dorina Coricovac; Cristina Dehelean; Iulia Pinzaru; Alexandra Mioc; Oana-Maria Aburel; Ioana Macasoi; George Draghici; Crina Petean; Codruta Soica; Madalina Boruga; Brigitha Vlaicu; Mirela Muntean. Assessment of Betulinic Acid Cytotoxicity and Mitochondrial Metabolism Impairment in a Human Melanoma Cell Line. International Journal of Molecular Sciences 2021, 22, 4870 .
AMA StyleDorina Coricovac, Cristina Dehelean, Iulia Pinzaru, Alexandra Mioc, Oana-Maria Aburel, Ioana Macasoi, George Draghici, Crina Petean, Codruta Soica, Madalina Boruga, Brigitha Vlaicu, Mirela Muntean. Assessment of Betulinic Acid Cytotoxicity and Mitochondrial Metabolism Impairment in a Human Melanoma Cell Line. International Journal of Molecular Sciences. 2021; 22 (9):4870.
Chicago/Turabian StyleDorina Coricovac; Cristina Dehelean; Iulia Pinzaru; Alexandra Mioc; Oana-Maria Aburel; Ioana Macasoi; George Draghici; Crina Petean; Codruta Soica; Madalina Boruga; Brigitha Vlaicu; Mirela Muntean. 2021. "Assessment of Betulinic Acid Cytotoxicity and Mitochondrial Metabolism Impairment in a Human Melanoma Cell Line." International Journal of Molecular Sciences 22, no. 9: 4870.
Females require at a certain period of life the administration or supplementation of specific hormones (estrogen, progesterone), for various needs, such as: prevention of unwanted pregnancies, decreased menstrual bleeding, dysmenorrhea and pelvic pain in endometriosis, alleviation of symptoms associated with menopause, regulation of certain skin processes related to acne or aging and others. Also, hormones could act as oncogenes being known eloquent examples of estrogens labeled both as promoters of cell specific alteration or as mutagenic agents. The use of hormones and exposure to solar radiation is expected to cause a number of adverse changes to the body, especially due to their association with malignant processes. The current study was purported as a basis for understanding certain processes that occur with the administration of hormones and exposure to ultraviolet B (UVB) radiation. The animal model was made on healthy adult female BALB∕c mice, which were separated into groups and treated with Ethinylestradiol (EES), Levonorgestrel (LNG) and their combination in the presence of UVB radiation. Changes in skin physiological parameters were analyzed by non-invasive methods, biochemical parameters related to changes in blood circulating system were evaluated by standard methods and histopathological analysis was conducted to point out the changes at the level of the internal body. Measurement of skin parameters such as erythema, melanin, skin hydration, has highlighted some changes in hormone-treated and exposed to UVB radiation groups which were significant only in the case of erythema. Biochemical parameters showed variations in terms of liver enzymes in groups treated with active substances. Histologically, aspects of internal organs revealed significant changes in the group treated with EES and LNG and exposed to UVB radiation.
Virgiliu Bogdan Şorop; Veronica Mădălina Borugă; Iulia Andreea Pînzaru; Ileana Ramona Barac; Crinela Utescu; Anca Laura Maghiari; Flavia Baderca; Lavinia Bălan; Maria Şorop-Florea; Victor Dumitraşcu; Doru Mihai Anastasiu; Sebastian Simu; Daniela Radu; Oana Suciu. Hormone treatment and UVB exposure influences on female mice regarding skin physiological parameters, biochemical parameters and organ histology. Romanian Journal of Morphology and Embryology 2021, 61, 879 -887.
AMA StyleVirgiliu Bogdan Şorop, Veronica Mădălina Borugă, Iulia Andreea Pînzaru, Ileana Ramona Barac, Crinela Utescu, Anca Laura Maghiari, Flavia Baderca, Lavinia Bălan, Maria Şorop-Florea, Victor Dumitraşcu, Doru Mihai Anastasiu, Sebastian Simu, Daniela Radu, Oana Suciu. Hormone treatment and UVB exposure influences on female mice regarding skin physiological parameters, biochemical parameters and organ histology. Romanian Journal of Morphology and Embryology. 2021; 61 (3):879-887.
Chicago/Turabian StyleVirgiliu Bogdan Şorop; Veronica Mădălina Borugă; Iulia Andreea Pînzaru; Ileana Ramona Barac; Crinela Utescu; Anca Laura Maghiari; Flavia Baderca; Lavinia Bălan; Maria Şorop-Florea; Victor Dumitraşcu; Doru Mihai Anastasiu; Sebastian Simu; Daniela Radu; Oana Suciu. 2021. "Hormone treatment and UVB exposure influences on female mice regarding skin physiological parameters, biochemical parameters and organ histology." Romanian Journal of Morphology and Embryology 61, no. 3: 879-887.
Despite the recent advances in the field of chemically synthetized pharmaceutical agents, nature remains the main supplier of bioactive molecules. The research of natural products is a valuable approach for the discovery and development of novel biologically active compounds possessing unique structures and mechanisms of action. Although their use belongs to the traditional treatment regimes, plant-derived compounds still cover a large portion of the current-day pharmaceutical agents. Their medical importance is well recognized in the field of oncology, especially as an alternative to the limitations of conventional chemotherapy (severe side effects and inefficacy due to the occurrence of multi-drug resistance). This review offers a comprehensive perspective of the first blockbuster chemotherapeutic agents of natural origin's (e.g. taxol, vincristine, doxorubicin) mechanism of action using 3D representation. In addition is portrayed the step-by-step evolution from preclinical to clinical evaluation of the most recently studied natural compounds with potent antitumor activity (e.g. resveratrol, curcumin, betulinic acid, etc.) in terms of anticancer mechanisms of action and the possible indications as chemotherapeutic or chemopreventive agents and sensitizers. Finally, this review describes several efficient platforms for the encapsulation and targeted delivery of natural compounds in cancer treatment
Cristina Dehelean; Iasmina Marcovici; Codruta Soica; Marius Mioc; Dorina Coricovac; Stela Iurciuc; Octavian Cretu; Iulia Pinzaru. Plant-Derived Anticancer Compounds as New Perspectives in Drug Discovery and Alternative Therapy. Molecules 2021, 26, 1109 .
AMA StyleCristina Dehelean, Iasmina Marcovici, Codruta Soica, Marius Mioc, Dorina Coricovac, Stela Iurciuc, Octavian Cretu, Iulia Pinzaru. Plant-Derived Anticancer Compounds as New Perspectives in Drug Discovery and Alternative Therapy. Molecules. 2021; 26 (4):1109.
Chicago/Turabian StyleCristina Dehelean; Iasmina Marcovici; Codruta Soica; Marius Mioc; Dorina Coricovac; Stela Iurciuc; Octavian Cretu; Iulia Pinzaru. 2021. "Plant-Derived Anticancer Compounds as New Perspectives in Drug Discovery and Alternative Therapy." Molecules 26, no. 4: 1109.
Rutin (Rut) is a natural flavonol, well-known for its broad-spectrum of therapeutic effects, including antioxidant and antitumoral activities; still, it has a reduced clinical outcome due to its limited solubility in aqueous solutions. To overcome this drawback, this study proposes a novel formulation for rutin as a proniosomal gel for cutaneous applications. The gel was prepared by coacervation phase-separation method and complies with the standard requirements in terms of particle size (140.5 ± 2.56 nm), zeta potential (−27.33 ± 0.09 mV), encapsulation capacity (> 50%), pH (7.002 ± 0.18) and rheological properties. The results showed high biocompatibility of the gel on the 3D reconstructed human epidermis model characterized by increased viability of the cells and a lack of irritant and phototoxic potential. The evaluations on 2D cells confirm the preferential cytotoxic effect of Rut on melanoma cells (IC50 value = 8.601 µM, nuclear fragmentation) compared to normal keratinocytes. Our data suggest that the proniosomal gel is a promising drug carrier for Rut in the management and prevention of skin disorders.
Iulia Pinzaru; Alina Tanase; Virgil Enatescu; Dorina Coricovac; Flavia Bociort; Iasmina Marcovici; Claudia Watz; Lavinia Vlaia; Codruta Soica; Cristina Dehelean. Proniosomal Gel for Topical Delivery of Rutin: Preparation, Physicochemical Characterization and In Vitro Toxicological Profile Using 3D Reconstructed Human Epidermis Tissue and 2D Cells. Antioxidants 2021, 10, 85 .
AMA StyleIulia Pinzaru, Alina Tanase, Virgil Enatescu, Dorina Coricovac, Flavia Bociort, Iasmina Marcovici, Claudia Watz, Lavinia Vlaia, Codruta Soica, Cristina Dehelean. Proniosomal Gel for Topical Delivery of Rutin: Preparation, Physicochemical Characterization and In Vitro Toxicological Profile Using 3D Reconstructed Human Epidermis Tissue and 2D Cells. Antioxidants. 2021; 10 (1):85.
Chicago/Turabian StyleIulia Pinzaru; Alina Tanase; Virgil Enatescu; Dorina Coricovac; Flavia Bociort; Iasmina Marcovici; Claudia Watz; Lavinia Vlaia; Codruta Soica; Cristina Dehelean. 2021. "Proniosomal Gel for Topical Delivery of Rutin: Preparation, Physicochemical Characterization and In Vitro Toxicological Profile Using 3D Reconstructed Human Epidermis Tissue and 2D Cells." Antioxidants 10, no. 1: 85.
Betulinic acid (BA), a natural compound with various health benefits including selective antitumor activity, has a limited applicability in vivo due to its poor water solubility and bioavailability. Thus, this study focused on obtaining a BA nano-sized formulation with improved solubility and enhanced antitumor activity using silver nanocolloids (SilCo and PEG_SilCo) as drug carriers. The synthesis was performed using a chemical method and the physicochemical characterization was achieved applying UV-Vis absorption, transmission electron microscopy (TEM), Raman and photon correlation spectroscopy (PCS). The biological evaluation was conducted on two in vitro experimental models—hepatocellular carcinoma (HepG2) and lung cancer (A549) cell lines. The physicochemical characterization showed the following results: an average hydrodynamic diameter of 32 nm for SilCo_BA and 71 nm for PEG_SilCo_BA, a spherical shape, and a loading capacity of 54.1% for SilCo_BA and 61.9% for PEG_SilCo_BA, respectively. The in vitro assessment revealed a cell type- and time-dependent cytotoxic effect characterized by a decrease in cell viability as follows: (i) SilCo_BA (66.44%) < PEG_SilCo_BA (72.05%) < BA_DMSO (75.30%) in HepG2 cells, and (ii) SilCo_BA (75.28%) < PEG_SilCo_BA (86.80%) < BA_DMSO (87.99%) in A549 cells. The novel silver nanocolloids loaded with BA induced an augmented anticancer effect as compared to BA alone.
Iulia Pinzaru; Cristian Sarau; Dorina Coricovac; Iasmina Marcovici; Crinela Utescu; Sergiu Tofan; Ramona Amina Popovici; Horatiu Cristian Manea; Ioana E. Pavel; Codruta Soica; Cristina Dehelean. Silver Nanocolloids Loaded with Betulinic Acid with Enhanced Antitumor Potential: Physicochemical Characterization and In Vitro Evaluation. Nanomaterials 2021, 11, 152 .
AMA StyleIulia Pinzaru, Cristian Sarau, Dorina Coricovac, Iasmina Marcovici, Crinela Utescu, Sergiu Tofan, Ramona Amina Popovici, Horatiu Cristian Manea, Ioana E. Pavel, Codruta Soica, Cristina Dehelean. Silver Nanocolloids Loaded with Betulinic Acid with Enhanced Antitumor Potential: Physicochemical Characterization and In Vitro Evaluation. Nanomaterials. 2021; 11 (1):152.
Chicago/Turabian StyleIulia Pinzaru; Cristian Sarau; Dorina Coricovac; Iasmina Marcovici; Crinela Utescu; Sergiu Tofan; Ramona Amina Popovici; Horatiu Cristian Manea; Ioana E. Pavel; Codruta Soica; Cristina Dehelean. 2021. "Silver Nanocolloids Loaded with Betulinic Acid with Enhanced Antitumor Potential: Physicochemical Characterization and In Vitro Evaluation." Nanomaterials 11, no. 1: 152.
Aspartame (ASP), an artificial sweetener abundantly consumed in recent years in an array of dietary products, has raised some concerns in terms of toxicity, and it was even suggested a link with the risk of carcinogenesis (colorectal cancer), though the present scientific data are rather inconclusive. This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. These data suggest new directions of research regarding aspartame’s role in colorectal cancer.
Anca Laura Maghiari; Dorina Coricovac; Iulia Andreea Pinzaru; Ioana Gabriela Macașoi; Iasmina Marcovici; Sebastian Simu; Dan Navolan; Cristina Dehelean. High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells. Nutrients 2020, 12, 3600 .
AMA StyleAnca Laura Maghiari, Dorina Coricovac, Iulia Andreea Pinzaru, Ioana Gabriela Macașoi, Iasmina Marcovici, Sebastian Simu, Dan Navolan, Cristina Dehelean. High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells. Nutrients. 2020; 12 (12):3600.
Chicago/Turabian StyleAnca Laura Maghiari; Dorina Coricovac; Iulia Andreea Pinzaru; Ioana Gabriela Macașoi; Iasmina Marcovici; Sebastian Simu; Dan Navolan; Cristina Dehelean. 2020. "High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells." Nutrients 12, no. 12: 3600.
The current study was aimed to evaluate the phenolic composition parameters of two hydro-alcoholic extracts of Ocimum basilicum L. (OB) obtained from the aerial part (without leaves) and leaves, in order to determine their contribution to the antioxidant activity (AOA). Both hydro-alcoholic extracts have proven to be rich in polyphenolic compounds, flavonoids, flavonols and tannins. Therefore, the leaves’ extracts reveal an inhibition percentage of 89%, almost comparable with the standard reference (95%). To complete the toxicological profile, the study also assessed the potential cytotoxicity of basil hydro-alcoholic extracts on immortalized human keratinocytes (HaCaT), skin human fibroblasts (1BR3), mice epidermis (JB6Cl41-5a) and primary human melanocytes (HEMa) cells, correlated to A375 antitumor in vitro activity. The extracts did not induce significant cytotoxic effect on any of the selected normal cell lines but showed relevant activity on A375 cells. Considering the low values obtained regarding the irritative effects in the chorionallantoic membrane of the egg on blood vessels, we can emphasize that both extracts can be considered as biocompatible ingredients. Regarding the potential activity of hydro-alcoholic extracts on human skin, the decrease of erythema values after the application of extracts was a relevant observation which indicates the anti-inflammatory potential of Ocimum basilicum L.
Alin Faur; Claudia Watz; Elena-Alina Moacă; Ştefana Avram; Florin Borcan; Iulia Pinzaru; Andrada Iftode; Mirela Nicolov; Ramona Amina Popovici; Marius Raica; Camelia A. Szuhanek; Cristina Dehelean. Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient. Molecules 2020, 25, 5442 .
AMA StyleAlin Faur, Claudia Watz, Elena-Alina Moacă, Ştefana Avram, Florin Borcan, Iulia Pinzaru, Andrada Iftode, Mirela Nicolov, Ramona Amina Popovici, Marius Raica, Camelia A. Szuhanek, Cristina Dehelean. Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient. Molecules. 2020; 25 (22):5442.
Chicago/Turabian StyleAlin Faur; Claudia Watz; Elena-Alina Moacă; Ştefana Avram; Florin Borcan; Iulia Pinzaru; Andrada Iftode; Mirela Nicolov; Ramona Amina Popovici; Marius Raica; Camelia A. Szuhanek; Cristina Dehelean. 2020. "Correlations on Phenolic Screening Related to In Vitro and In Ovo Assessment of Ocimum basilicum L. Hydro-Alcoholic Extracts Used as Skin Active Ingredient." Molecules 25, no. 22: 5442.
Although it recorded a breakthrough for the treatment of metastatic melanoma in the last decade, this disease remains a challenge in terms of finding an efficient treatment, reducing secondary resistance to treatment, and understanding the complexity of the molecular mechanisms involved in its development, progression and metastasis. This study aims to verify the multitarget effect of a natural compound, betulinic acid (BA), a pentacyclic triterpene, as antimelanoma agent by applying two experimental models: a human melanoma cell line—A375 and the chick chorioallantoic membrane model. The methods applied in this study were: MTT cell viability assay for cytotoxicity assessment and the chorioallantoic membrane assay (CAM) for antiangiogenic evaluation. The results indicated a significant decrease of A375 cells viability after a 72 h BA treatment even at the lowest concentration tested—1 µM (61.95% viable cells), with a calculated IC50 of 9.437 µM. In addition, BA inhibited not only the in ovo A375-induced tumor growth but also the angiogenesis on the primary site at 72 h post application. These data highlight the potential antimelanoma effect of BA by targeting the tumor cells via multiples pathways as inducing cell death and suppressing the angiogenic process, a must have for tumor development.
Dorina Coricovac; Iulia Pînzaru; Ștefana Avram; Ioana Macașoi; Codruța Șoica; Cristina Dehelean. In Vitro and In Ovo Assessment of Betulinic Acid Antimelanoma Effect. Timisoara Medical Journal 2020, 2020, 1 .
AMA StyleDorina Coricovac, Iulia Pînzaru, Ștefana Avram, Ioana Macașoi, Codruța Șoica, Cristina Dehelean. In Vitro and In Ovo Assessment of Betulinic Acid Antimelanoma Effect. Timisoara Medical Journal. 2020; 2020 (1):1.
Chicago/Turabian StyleDorina Coricovac; Iulia Pînzaru; Ștefana Avram; Ioana Macașoi; Codruța Șoica; Cristina Dehelean. 2020. "In Vitro and In Ovo Assessment of Betulinic Acid Antimelanoma Effect." Timisoara Medical Journal 2020, no. 1: 1.
Purpose: Breast cancer presents one of the highest rates of prevalence around the world. Despite this, the current breast cancer therapy is characterized by significant side effects and high risk of recurrence. The present work aimed to develop a new therapeutic strategy that may improve the current breast cancer therapy by developing a heat-sensitive liposomal nano-platform suitable to incorporate both anti-tumor betulinic acid (BA) compound and magnetic iron nanoparticles (MIONPs), in order to address both remote drug release and hyperthermia-inducing features. To address the above-mentioned biomedical purposes, the nanocarrier must possess specific features such as specific phase transition temperature, diameter below 200 nm, superparamagnetic properties and heating capacity. Moreover, the anti-tumor activity of the developed nanocarrier should significantly affect human breast adenocarcinoma cells. Methods: BA-loaded magnetoliposomes and corresponding controls (BA-free liposomes and liposomes containing no magnetic payload) were obtained through the thin-layer hydration method. The quality and stability of the multifunctional platforms were physico-chemically analysed by the means of RAMAN, scanning electron microscopy-EDAX, dynamic light scattering, zeta potential and DSC analysis. Besides this, the magnetic characterization of magnetoliposomes was performed in terms of superparamagnetic behaviour and heating capacity. The biological profile of the platforms and controls was screened through multiple in vitro methods, such as MTT, LDH and scratch assays, together with immunofluorescence staining. In addition, CAM assay was performed in order to assess a possible anti-angiogenic activity induced by the test samples. Results: The physico-chemical analysis revealed that BA-loaded magnetoliposomes present suitable characteristics for the purpose of this study, showing biocompatible phase transition temperature, a diameter of 198 nm, superparamagnetic features and heating capacity. In vitro results showed that hyperthermia induces enhanced anti-tumor activity when breast adenocarcinoma MDA-MB-231 cells were exposed to BA-loaded magnetoliposomes, while a low cytotoxic rate was exhibited by the non-tumorigenic breast epithelial MCF 10A cells. Moreover, the in ovo angiogenesis assay endorsed the efficacy of this multifunctional platform as a good strategy for breast cancer therapy, under hyperthermal conditions. Regarding the possible mechanism of action of this multifunctional nano-platform, the immunocytochemistry of the MCF7 and MDA-MB-231 breast carcinoma cells revealed a microtubule assembly modulatory activity, under hyperthermal conditions. Conclusion: Collectively, these findings indicate that BA-loaded magnetoliposomes, under hyperthermal conditions, might serve as a promising strategy for breast adenocarcinoma treatment.
Claudia Geanina Farcas; Cristina Dehelean; Iulia Andreea Pinzaru; Marius Mioc; Vlad Socoliuc; Elena-Alina Moaca; Stefana Avram; Roxana Ghiulai; Dorina Coricovac; Ioana Pavel; Praveen Kumar Alla; Octavian Marius Cretu; Codruta Soica; Felicia Loghin. Thermosensitive Betulinic Acid-Loaded Magnetoliposomes: A Promising Antitumor Potential for Highly Aggressive Human Breast Adenocarcinoma Cells Under Hyperthermic Conditions. International Journal of Nanomedicine 2020, ume 15, 8175 -8200.
AMA StyleClaudia Geanina Farcas, Cristina Dehelean, Iulia Andreea Pinzaru, Marius Mioc, Vlad Socoliuc, Elena-Alina Moaca, Stefana Avram, Roxana Ghiulai, Dorina Coricovac, Ioana Pavel, Praveen Kumar Alla, Octavian Marius Cretu, Codruta Soica, Felicia Loghin. Thermosensitive Betulinic Acid-Loaded Magnetoliposomes: A Promising Antitumor Potential for Highly Aggressive Human Breast Adenocarcinoma Cells Under Hyperthermic Conditions. International Journal of Nanomedicine. 2020; ume 15 ():8175-8200.
Chicago/Turabian StyleClaudia Geanina Farcas; Cristina Dehelean; Iulia Andreea Pinzaru; Marius Mioc; Vlad Socoliuc; Elena-Alina Moaca; Stefana Avram; Roxana Ghiulai; Dorina Coricovac; Ioana Pavel; Praveen Kumar Alla; Octavian Marius Cretu; Codruta Soica; Felicia Loghin. 2020. "Thermosensitive Betulinic Acid-Loaded Magnetoliposomes: A Promising Antitumor Potential for Highly Aggressive Human Breast Adenocarcinoma Cells Under Hyperthermic Conditions." International Journal of Nanomedicine ume 15, no. : 8175-8200.
SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) pandemic represents the primary public health concern nowadays, and great efforts are made worldwide for efficient management of this crisis. Considerable scientific progress was recorded regarding SARS-CoV-2 infection in terms of genomic structure, diagnostic tools, viral transmission, mechanism of viral infection, symptomatology, clinical impact, and complications, but these data evolve constantly. Up to date, neither an effective vaccine nor SARS-CoV-2 specific antiviral agents have been approved, but significant advances were enlisted in this direction by investigating repurposed approved drugs (ongoing clinical trials) or developing innovative antiviral drugs (preclinical and clinical studies). This review presents a thorough analysis of repurposed drug admitted for compassionate use from a chemical structure—biological activity perspective highlighting the ADME (absorption, distribution, metabolism, and excretion) properties and the toxicophore groups linked to potential adverse effects. A detailed pharmacological description of the novel potential anti-COVID-19 therapeutics was also included. In addition, a comprehensible overview of SARS-CoV-2 infection in terms of general description and structure, mechanism of viral infection, and clinical impact was portrayed.
Cristina Adriana Dehelean; Voichita Lazureanu; Dorina Coricovac; Marius Mioc; Roxana Oancea; Iasmina Marcovici; Iulia Pinzaru; Codruta Soica; Aristidis M. Tsatsakis; Octavian Cretu. SARS-CoV-2: Repurposed Drugs and Novel Therapeutic Approaches—Insights into Chemical Structure—Biological Activity and Toxicological Screening. Journal of Clinical Medicine 2020, 9, 2084 .
AMA StyleCristina Adriana Dehelean, Voichita Lazureanu, Dorina Coricovac, Marius Mioc, Roxana Oancea, Iasmina Marcovici, Iulia Pinzaru, Codruta Soica, Aristidis M. Tsatsakis, Octavian Cretu. SARS-CoV-2: Repurposed Drugs and Novel Therapeutic Approaches—Insights into Chemical Structure—Biological Activity and Toxicological Screening. Journal of Clinical Medicine. 2020; 9 (7):2084.
Chicago/Turabian StyleCristina Adriana Dehelean; Voichita Lazureanu; Dorina Coricovac; Marius Mioc; Roxana Oancea; Iasmina Marcovici; Iulia Pinzaru; Codruta Soica; Aristidis M. Tsatsakis; Octavian Cretu. 2020. "SARS-CoV-2: Repurposed Drugs and Novel Therapeutic Approaches—Insights into Chemical Structure—Biological Activity and Toxicological Screening." Journal of Clinical Medicine 9, no. 7: 2084.
In the last decade, the incidence of skin cancer has increased significantly and there is data that rank it as the most common form of malignancy in humans. Among the classified skin cancers, melanoma is the most severe with a significant mortality rate. The determining factor in the occurrence of skin cancer is generally recognised to be ultraviolet radiation. Skin pigmentation has been shown to provide a high degree of protection and this is due to melanin. In order to be able to consider certain hypotheses and to develop new approaches to melanoma, in this study it was studied the melanin content in certain cell types that have a direct connection with melanoma. The cell lines used in the study were: (i) human melanoma cell lines: A375, SK-MEL-1, SK-MEL-3, SK-MEL-5, SK-MEL-28, SH-4, and COLO 829; (ii) mouse melanoma cell lines: B164A5, B16F0, and B16F10; and (iii) healthy cell lines of human origin: HEMa (primary melanocytes), HaCaT (immortalised keratinocytes) and 1BR3 (human fibroblasts). The results obtained pointed out that A375, SK-MEL-28 and SK-MEL-5 cells do not produce melanin, SK-MEL-1, SH-4 and SK-MEL-3 cells produce melanin while COLO 829 cells � only in small passages produce melanin. Of the 3 lines of murine melanoma tested, the one that produces melanin in a higher concentration is B16F10, followed by B16F0 and B164A5.
Tiberiu Bratu; Mircea Berceanu; Dorina Coricovac; Zorin Crainiceanu; Zsolt Gyori; Andreea Roman; Iasmina Marcovici; Cristina Dehelean; Iulia Andreea Pinzaru. Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma. Revista de Chimie 2020, 71, 430 -437.
AMA StyleTiberiu Bratu, Mircea Berceanu, Dorina Coricovac, Zorin Crainiceanu, Zsolt Gyori, Andreea Roman, Iasmina Marcovici, Cristina Dehelean, Iulia Andreea Pinzaru. Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma. Revista de Chimie. 2020; 71 (4):430-437.
Chicago/Turabian StyleTiberiu Bratu; Mircea Berceanu; Dorina Coricovac; Zorin Crainiceanu; Zsolt Gyori; Andreea Roman; Iasmina Marcovici; Cristina Dehelean; Iulia Andreea Pinzaru. 2020. "Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma." Revista de Chimie 71, no. 4: 430-437.
A new class of magnetite (Fe3O4) particles, coined as “Single Crystalline Micrometric Iron Oxide Particles” (SCMIOPs), were obtained by hydrothermal synthesis. Both the single Fe3O4 phase content and the particle sizes range, from 1 µm to 30 µm, can be controlled by synthesis. The notable finding states that these particles exhibit vanishing remanent magnetization (σr=0.28 emu/g) and coercive force (Hc=1.5 Oe), which indicate a superparamagnetic-like behavior (unexpected at micrometric particles size), and remarkably high saturation magnetization (σs=95.5 emu/g), what ensures strong magnetic response, and the lack of agglomeration after the magnetic field removal. These qualities make such particles candidates for biomedical applications, to be used instead of magnetic nanoparticles which inevitably involve some drawbacks like aglommeration and insufficient magnetic response. In this sense, cytocompatibility/cytotoxicity tests were performed on human cells, and the results have clearly indicated that SCMIOPs are cytocompatible for healthy cell lines HaCaT (human keratinocytes) and HEMa (primary epidermal melanocytes) and cytotoxic for neoplastic cell lines A375 (human melanoma) and B164A5 (murine melanoma) in a dose-dependent manner.
Claudia Geanina Farcas; Ioana Macasoi; Iulia Pinzaru; Marius Chirita; Marius Constantin Chirita Mihaila; Cristina Dehelean; Stefana Avram; Felicia Loghin; Liviu Mocanu; Virgil Rotaru; Adrian Ieta; Aurel Ercuta; Dorina Coricovac. Controlled Synthesis and Characterization of Micrometric Single Crystalline Magnetite With Superparamagnetic Behavior and Cytocompatibility/Cytotoxicity Assessments. Frontiers in Pharmacology 2020, 11, 410 .
AMA StyleClaudia Geanina Farcas, Ioana Macasoi, Iulia Pinzaru, Marius Chirita, Marius Constantin Chirita Mihaila, Cristina Dehelean, Stefana Avram, Felicia Loghin, Liviu Mocanu, Virgil Rotaru, Adrian Ieta, Aurel Ercuta, Dorina Coricovac. Controlled Synthesis and Characterization of Micrometric Single Crystalline Magnetite With Superparamagnetic Behavior and Cytocompatibility/Cytotoxicity Assessments. Frontiers in Pharmacology. 2020; 11 ():410.
Chicago/Turabian StyleClaudia Geanina Farcas; Ioana Macasoi; Iulia Pinzaru; Marius Chirita; Marius Constantin Chirita Mihaila; Cristina Dehelean; Stefana Avram; Felicia Loghin; Liviu Mocanu; Virgil Rotaru; Adrian Ieta; Aurel Ercuta; Dorina Coricovac. 2020. "Controlled Synthesis and Characterization of Micrometric Single Crystalline Magnetite With Superparamagnetic Behavior and Cytocompatibility/Cytotoxicity Assessments." Frontiers in Pharmacology 11, no. : 410.
There is currently limited scientific evidence linking soil copper and land snails, although these invertebrates are important players in terrestrial ecosystems. In the present study, Cantareus aspersus juveniles, were exposed in two successive phases of 30 days each, to soil spiked with increasing concentrations of copper sulfate. Copper concentrated preferentially and in a dose-dependent manner in the hepatopancreas. In the case of specimens previously exposed to Cu-spiked soils, Cu retention kinetics were independent from the effects of a new exposure event. There was no effect on shell growth, but significant mortality was observed at 60 days. The no observed effect concentration and the lowest observed effect concentration for mortality in snails, were ˜ 41 and 54 mg, respectively, per grams dry weight in the hepatopancreas. The results demonstrate, for the first time, that terrestrial gastropods can accumulate soil Cu autonomously from dietary uptake.
George Andrei Drăghici; Cristina Dehelean; Iulia Pinzaru; Despina Maria Bordean; Aurica Borozan; Aristides Michael Tsatsakis; Leda Kovatsi; Dragos Nica. Soil copper uptake by land snails: A semi-field experiment with juvenile Cantareus aspersus snails. Environmental Toxicology and Pharmacology 2019, 72, 103243 .
AMA StyleGeorge Andrei Drăghici, Cristina Dehelean, Iulia Pinzaru, Despina Maria Bordean, Aurica Borozan, Aristides Michael Tsatsakis, Leda Kovatsi, Dragos Nica. Soil copper uptake by land snails: A semi-field experiment with juvenile Cantareus aspersus snails. Environmental Toxicology and Pharmacology. 2019; 72 ():103243.
Chicago/Turabian StyleGeorge Andrei Drăghici; Cristina Dehelean; Iulia Pinzaru; Despina Maria Bordean; Aurica Borozan; Aristides Michael Tsatsakis; Leda Kovatsi; Dragos Nica. 2019. "Soil copper uptake by land snails: A semi-field experiment with juvenile Cantareus aspersus snails." Environmental Toxicology and Pharmacology 72, no. : 103243.
Bisphosphonates are effective antiresorptive agents frequently used in the treatment of different bone disorders, as osteoporosis, Paget�s disease and tumours that cause osteolysis. A major concern related to bisphosphonates therapy is represented by osteonecrosis of jaw, a serious, debilitating, and mostly, a therapy-resistant disease, reported as a frequent side effect of bisphosphonates. The present study was aimed to assess and compare the effect of four commercially available bisphosphonates, very frequently used as oral (Fosamax - F and Actonel - A) and injectable (Ossica - O and Zoledronic acid - Z) therapy on primary human gingival fibroblasts - HGF viability. Alamar blue cell viability assay was performed to assess the effect of test compounds (1.5; 2.5; 5 and 10 mM) on gingival fibroblasts viability after a 24 h interval. In these experimental conditions, injectable bisphosphonates (O and Z) proved to be safe for HGF cells, whereas oral compounds (F and A) were cytotoxic even at low concentrations.
Octavia Iulia Balean; Alin Daniel Floare; Roxanne Focht; Daniela Jumanca; Dorina Coricovac; Iulia Andreea Pinzaru; Atena Galuscan; Doina Chioran; Adelina Cheveresan; Angela Codruta Podariu. Comparative Effects of Oral and Injectable Bisphosphonates in Primary Human Gingival Fibroblasts. Revista de Chimie 2019, 70, 3325 -3329.
AMA StyleOctavia Iulia Balean, Alin Daniel Floare, Roxanne Focht, Daniela Jumanca, Dorina Coricovac, Iulia Andreea Pinzaru, Atena Galuscan, Doina Chioran, Adelina Cheveresan, Angela Codruta Podariu. Comparative Effects of Oral and Injectable Bisphosphonates in Primary Human Gingival Fibroblasts. Revista de Chimie. 2019; 70 (9):3325-3329.
Chicago/Turabian StyleOctavia Iulia Balean; Alin Daniel Floare; Roxanne Focht; Daniela Jumanca; Dorina Coricovac; Iulia Andreea Pinzaru; Atena Galuscan; Doina Chioran; Adelina Cheveresan; Angela Codruta Podariu. 2019. "Comparative Effects of Oral and Injectable Bisphosphonates in Primary Human Gingival Fibroblasts." Revista de Chimie 70, no. 9: 3325-3329.
Maslinic acid is a pentacyclic triterpene with a plethora of biological activities, including anti-inflammatory, antioxidant, antimicrobial, cardioprotective, and antitumor effects. New derivatives with improved properties and broad-spectrum activity can be obtained following structural changes of the compound. The present study was aimed to characterize a benzylamide derivative of maslinic acid—benzyl (2α, 3β) 2,3-diacetoxy-olean−12-en-28-amide (EM2)—with respect to the anti-angiogenic and anti-inflammatory effects in two in vivo experimental models. Consequently, the compound showed good tolerability and lack of irritation in the chorioallantoic membrane assay with no impairment of the normal angiogenic process during the tested stages of development. In the acute ear inflammation murine model, application of EM2 induced a mild anti-inflammatory effect that was potentiated by the association with zinc chloride (ZnCl2). A decrease in dermal thickness of mice ears was observed when EM2 and ZnCl2 were applied separately or in combination. Moreover, hyalinization of the dermis appeared only when EM2 was associated with ZnCl2, strongly suggesting the role of their combination in wound healing.
Ioana Zinuca Pavel; Rene Csuk; Corina Danciu; Stefana Avram; Flavia Baderca; Andreea Cioca; Elena-Alina Moacă; Ciprian-Valentin Mihali; Iulia Pinzaru; Danina Mirela Muntean; Cristina Adriana Dehelean. Assessment of the Antiangiogenic and Anti-Inflammatory Properties of a Maslinic Acid Derivative and its Potentiation using Zinc Chloride. International Journal of Molecular Sciences 2019, 20, 2828 .
AMA StyleIoana Zinuca Pavel, Rene Csuk, Corina Danciu, Stefana Avram, Flavia Baderca, Andreea Cioca, Elena-Alina Moacă, Ciprian-Valentin Mihali, Iulia Pinzaru, Danina Mirela Muntean, Cristina Adriana Dehelean. Assessment of the Antiangiogenic and Anti-Inflammatory Properties of a Maslinic Acid Derivative and its Potentiation using Zinc Chloride. International Journal of Molecular Sciences. 2019; 20 (11):2828.
Chicago/Turabian StyleIoana Zinuca Pavel; Rene Csuk; Corina Danciu; Stefana Avram; Flavia Baderca; Andreea Cioca; Elena-Alina Moacă; Ciprian-Valentin Mihali; Iulia Pinzaru; Danina Mirela Muntean; Cristina Adriana Dehelean. 2019. "Assessment of the Antiangiogenic and Anti-Inflammatory Properties of a Maslinic Acid Derivative and its Potentiation using Zinc Chloride." International Journal of Molecular Sciences 20, no. 11: 2828.
Satureja hortensis L. presents an increased interest due to its chemical composition, abundant in monoterpenes, aglyconic and glycosylates flavonoids, and phenolic acids, leading to important biological activity. The present study compared the biological activity of volatile oil (VO) and total hydro-alcoholic extract (TE) of Satureja hortensis L. in terms of: i) antioxidant activity; ii) antimicrobial activity; and iii) viability, migration and proliferation on two healthy cell lines (keratinocytes-HaCaT and fibroblasts-1BR3) and two melanoma cell lines (human-A375 and murine-B164A5). Antioxidant activity of VO and TE showed maximal values around 72%. Antimicrobial screening highlighted the inhibitory capacity of VO against all seven tested bacteria strains, with the most pronounced effect against S. aureus and C. albicans, while TE exerted only a slight activity against three bacteria strains. VO showed greater efficacy than TE on both tumor cell lines (A375 and B164A5), the activity of the compounds was higher when low concentrations were used (5, 10 and 25 µM) while at high concentrations (50 and 100 µM) the percentages of viability were increased.
Ramona A. Popovici; Delia Vaduva; Iulia Pinzaru; Cristina A. Dehelean; Claudia G. Farcas; Dorina Coricovac; Corina Danciu; Iuliana Popescu; Ersilia Alexa; Voichita Lazureanu; Horia T. Stanca. A comparative study on the biological activity of essential oil and total hydro-alcoholic extract of Satureja hortensis L. Experimental and Therapeutic Medicine 2019, 18, 932 -942.
AMA StyleRamona A. Popovici, Delia Vaduva, Iulia Pinzaru, Cristina A. Dehelean, Claudia G. Farcas, Dorina Coricovac, Corina Danciu, Iuliana Popescu, Ersilia Alexa, Voichita Lazureanu, Horia T. Stanca. A comparative study on the biological activity of essential oil and total hydro-alcoholic extract of Satureja hortensis L. Experimental and Therapeutic Medicine. 2019; 18 (2):932-942.
Chicago/Turabian StyleRamona A. Popovici; Delia Vaduva; Iulia Pinzaru; Cristina A. Dehelean; Claudia G. Farcas; Dorina Coricovac; Corina Danciu; Iuliana Popescu; Ersilia Alexa; Voichita Lazureanu; Horia T. Stanca. 2019. "A comparative study on the biological activity of essential oil and total hydro-alcoholic extract of Satureja hortensis L." Experimental and Therapeutic Medicine 18, no. 2: 932-942.
Introduction Many experimental and clinical trials have suggested that flaxseed might be a potent antihypertensive, but the evidence concerning the effects of flaxseed supplements on plasma C-reactive protein (CRP) concentrations has not been fully conclusive. We assessed the impact of the...
Sorin Ursoniu; Amirhossein Sahebkar; Maria-Corina Serban; Iulia Andreea Pinzaru; Cristina Dehelean; Lavinia Noveanu; Jacek Rysz; Maciej Banach; Lipid and Blood Pressure Meta-analysis Collaboration (LBPMC) Group. A systematic review and meta-analysis of clinical trials investigating the effects of flaxseed supplementation on plasma C-reactive protein concentrations. Archives of Medical Science 2019, 15, 12 -22.
AMA StyleSorin Ursoniu, Amirhossein Sahebkar, Maria-Corina Serban, Iulia Andreea Pinzaru, Cristina Dehelean, Lavinia Noveanu, Jacek Rysz, Maciej Banach, Lipid and Blood Pressure Meta-analysis Collaboration (LBPMC) Group. A systematic review and meta-analysis of clinical trials investigating the effects of flaxseed supplementation on plasma C-reactive protein concentrations. Archives of Medical Science. 2019; 15 (1):12-22.
Chicago/Turabian StyleSorin Ursoniu; Amirhossein Sahebkar; Maria-Corina Serban; Iulia Andreea Pinzaru; Cristina Dehelean; Lavinia Noveanu; Jacek Rysz; Maciej Banach; Lipid and Blood Pressure Meta-analysis Collaboration (LBPMC) Group. 2019. "A systematic review and meta-analysis of clinical trials investigating the effects of flaxseed supplementation on plasma C-reactive protein concentrations." Archives of Medical Science 15, no. 1: 12-22.
The drug carriers represent a very important and modern pathway used to increase the therapeutic efficacy of the natural active substances. Nano-sized assemblies of noble metals and herbal extracts represent very simple, cheap, biologically and ecologically friendly synthesis. The main aims of this study were to obtain polyurethane nanovesicles containing silver nanoparticles and to evaluate their release rate. The vesicles� sizes, homogeneity and surface charge were studied using a Cordouan Technol. Zetasizer, while the thermal behaviour was assessed by DSC. UV-Vis measurements were done to evaluate the release rate. Toxicological effects were studied by non-invasive techniques on human skin. The results suggest the obtaining of homogenous polyurethane nanovesicles between 80 and 95 nm, with positive surface charge, very good thermal stability and a prolonged release. The non-irritation potential recommends these vesicles as a safe carrier for different herbal extracts.
Maria Proks; Florin Borcan; Adelina Cheveresan; Iulia Andreea Pinzaru; Bogdan Almajan Guta; Dorina Coricovac; Virgil Paunescu; Voichita Lazureanu. Study on the Release and Bioevaluations of Green Silver Nanoparticles Entrapped Inside Polymer-based Nanovesicles. Materiale Plastice 2018, 55, 696 -699.
AMA StyleMaria Proks, Florin Borcan, Adelina Cheveresan, Iulia Andreea Pinzaru, Bogdan Almajan Guta, Dorina Coricovac, Virgil Paunescu, Voichita Lazureanu. Study on the Release and Bioevaluations of Green Silver Nanoparticles Entrapped Inside Polymer-based Nanovesicles. Materiale Plastice. 2018; 55 (4):696-699.
Chicago/Turabian StyleMaria Proks; Florin Borcan; Adelina Cheveresan; Iulia Andreea Pinzaru; Bogdan Almajan Guta; Dorina Coricovac; Virgil Paunescu; Voichita Lazureanu. 2018. "Study on the Release and Bioevaluations of Green Silver Nanoparticles Entrapped Inside Polymer-based Nanovesicles." Materiale Plastice 55, no. 4: 696-699.