Raulí is one of the most emblematic tree species of the Chilean temperate forests. Due to the high quality wood, this tree has been used for furniture and handicrafts manufacturing, which has positioned raulí as one of the most important commercial timber species in Chile. Currently, the international market demands sustainable production system for forest production, more specifically in plantlets production. In this regard, plant growth-promoting rhizobacteria (PGPR) inoculants may enhance the growth and survival of plantlets in nurseries, which means an increase in the effectiveness of replanting operations. Therefore, the aim of the present study was to isolate, characterize and screen rhizosphere-associated bacteria with PGPR potential, isolated from raulí that growth in volcanic soils in southern Chile. A total of 1,261 bacterial strains were isolated from different volcanic soils. Out of 1,261 isolates, 100 were selected based on their high levels of indole acetic acid (IAA) production. These isolates were then subjected to screening for 1-aminocyclopropane-1-carboxylic acid deaminase activity, and their ability to fix nitrogen was determined. From the 100 selected isolates, 7 were chosen for producing the highest amount of IAA to continue with genetic characterization based on their 16S rRNA gene sequences. These 7 isolates were characterized as members of the Serratia genus and were used to develop multi-strain inoculant mixtures. Later, a nursery study followed to determine the effect of inoculation with the Serratia strains on the growth of RA88 raulí clone plantlets. The nursery experiment demonstrated that Serratia strains have the potential to increase the root collar diameter, height, relative chlorophyll content, biomass and nitrogen content of raulí plantlets. The study concluded, that Serratia strains have the potential to be used as biofertilizers to increase plant growth in nursery conditions.

Intensive agricultural management affects the physical, chemical, and biological properties of soil, potentially contributing to a decrease in soil carbon storage. In this study, the effects of soil management intensity on soil organic carbon (SOC) content and its labile fractions, i.e., water-soluble organic carbon (OC-sol) and permanganate oxidizable carbon (POXC), were evaluated in a volcanic-ash-derived soil (Andisol) with a very high soil organic matter (SOM) content (>20%). These indicators were associated with water-stable aggregates (WSAs) and biological indicators, namely, earthworm density, cellulase activity, and autoclaved-citrate-extractable (ACE) proteins, related to the decomposition of SOM and its physical protection. The conditions evaluated were secondary native forest (SF), naturalized grassland (NG), no-till (NT), and conventional tillage (CT), considering the last item to be representative of a higher agriculture management intensity. Soil samples were collected by horizon. The SF and NG soil showed higher contents of SOC, OC-sol, and POXC. When comparing the evaluated annual cropping systems, NT showed higher values than CT (p < 0.05) in the first horizon (Hz1), while similar values were found at deeper horizons. The highest cellulase activity, ACE protein levels, and earthworm densities were found in NG and SF. NT also showed significantly higher levels of the aforementioned factors than CT (p < 0.05). A positive and significant relationship was found between the SOC content and WSA (R² = 0.76; p < 0.05) in the whole profile and between POXC and WSA for Hz1 (R² = 0.67; p < 0.05). Soil C storage was affected by the intensity of agricultural management, mainly because of the effect of tillage on structural stability, considering that biological activity synthesizes compounds such as enzymes and proteins that react and adhere to the mineral fraction affecting aggregate stability. The C content stored in the soil is consequently a key indicator with which to regulate SOM and protect SOC.

Despite the global expansion of forest plantations in Chile, their effect on biology properties of soil has still been only scarcely studied. Land use change in the Chilean Coastal Cordillera (36° to 40° S) is mainly attributed to the conversion of native forest to agriculture and forest plantations (Eucalyptus globulus and Pinus radiata de Don). The aim of this paper was to evaluate the changes in microbial composition (PCR-DGGE) and enzyme activity after the substitution of a native forest (e.g., Nothofagus spp.) by fast-growing exotic species and cropping. The most important factors that influence the abundance and diversity of bacteria and the fungi community were the soil organic matter (SOM) content, phosphorous (P-Olsen), calcium (Ca), boron (B), and water-holding capacity. These variables can better predict the microbial community composition and its enzymatic activity in the surface Ah horizon. Land use change also affected chemical soil properties of biogeochemical cycles. However, to deeply understand the connection between chemical and physical soil factors and microbial community composition, more research is needed. On the other hand, the expansion of forest plantations in Chile should be subject to legislation aimed to protect the biological legacy as a strategy for forest productivity as well as the soil microbial biodiversity.