Zinc and its derivatives requirement increased to enhance human immunity against the different pandemics, including covid-19. Green synthesis is an emerging field of research. Zinc oxide (ZnO) nanoparticles have been prepared from Anoectochilus elatus and characterized using absorption, vibrational and electron microscope analysis. They were carried for antibacterial, inflammatory control tendency, and potential antioxidant activities. The brine shrimp lethal assay tested the biologically derived nanomaterial toxicity and the lethal concentration (LC₅₀) is 599.79 µg/ml. The inhibition against the important disease-causing pathogens was measured against four-gram negative, gram-positive bacteria and two fungus pathogens. The nanomaterial exposed inhibition zone for gram-positive bacteria between 17 mm and 25 mm. The inhibition zone against gram-negative bacteria exists between 19 mm and 24 mm. The anti-inflammatory activity was assessed by inhibition of protein denaturation and protease inhibitory activity using nanomaterial. The antioxidant activity was examined using four assays for the therapeutic activities. The average size range of 60–80 nm nanoparticles has prepared and exposed the good biological activity between 50 µg/ml and 100 µg/ml. The comparative results of anti-inflammatory and antioxidant assay results with standards such as Aspirin and vitamin C exposed that two to three times higher concentrations are required for the fifty percent of inhibitions. The prepared low-cost nanoparticle has exhibited excellent biological activity without any side effects and may enhance immunity.

This current work revealed a single-step fabrication of tungsten oxide nanoflakes (WO₃ NFs) with the help of Terminalia arjuna bark extract. Bioactive phytoconstituents of T. arjuna bark extract were involved in the nucleation process and promoted the material crystalline growth in a particular direction. The as-prepared sample thermal decomposition was analyzed by TG/DTG. The as-prepared sample was annealed at 300 °C for 2 h, and the annealed sample was characterized by UV-Vis-DRS, FTIR, Raman, XRD, SEM, EDX, and TEM. Synthesized WO₃ samples showed a monoclinic phase of the flake-like structure with lengths of 25~230 nm and diameters of 25~120 nm. The WO₃ NFs were evaluated against S. aureus and E. coli. Over 3 mg concentrations of WO₃ NFs outperform the positive control in antibacterial activity. The pseudo-first-order kinetics of the WO₃ NFs enhanced the photocatalytic performance of methylene blue (MB). These results prove that WO₃ NFs have sustainable performance in antibacterial and MB degradation applications.