Background. Pasteurella species are frequently encountered as serious diseases in small ruminants. It is the main cause of respiratory pasteurellosis in sheep and goats of all age groups. Methods. The cross-sectional study was conducted from December 2022 to April 2023 in Haramaya district, eastern Ethiopia, to isolate and identify Pasteurella multocida and Mannheimia haemolytica and estimate their prevalence, associated risk factors, and antimicrobial sensitivity of isolates in small ruminants using a purposive sampling method. A total of 384 samples (156 nasal swabs from clinic cases and 228 lung swabs from abattoir cases) were collected. STATA 14 software was used to analyze the data. In addition, multivariable logistic regression analysis was performed to assess an association of risk factors. Results. Out of the 384 samples examined, 164 were positive for pasteurellosis, resulting in a 42.70% prevalence. Similarly, 63 (38.4%) of the 164 positive results were from nasal swabs, while 101 (61.6%) came from lung samples. M. haemolytica accounted for 126 (76.82%) of the isolates, while P. multocida accounted for 38 (23.17%). Of the 63 nasal swab isolates, 33 (37%) were from goats and 30 (42.8%) were from sheep. And 17 (10.89%) and 46 (29.58%), respectively, were P. multocida and M. haemolytica. Of the 46 (40%) of the 101 (44.3%) isolates of the pneumonic lung, samples were from goats, while 55 (48.47%) were from sheep. In this study, the risk factors (species, age, and body condition score) were found to be significant ( ). Pasteurella isolates evaluated for antibiotic susceptibility were highly resistant to oxacillin (90.90%), followed by gentamycin (72.72%), and penicillin (63.63%). However, the isolates were highly sensitive to chloramphenicol (90.90%), followed by tetracycline (63.63%), and ampicillin (54.54%). Conclusion. This study showed that M. haemolytica and P. multocida are the common causes of mannheimiosis and pasteurellosis in small ruminants, respectively, and isolates were resistant to commonly used antibiotics in the study area. Thus, an integrated vaccination strategy, antimicrobial resistance monitoring, and avoidance of stress-inducing factors are recommended.

Salmonella infections are most commonly found in animal-derived foods. From December 2021 to May 2022, the researchers conducted a cross-sectional study to determine the prevalence of Salmonella isolated from raw milk collected in and around Areka town, Boloso Sore Woreda, Wolaita Zone, Southern Ethiopia. A total of 151 direct udder milk samples were collected at random and examined using bacteriological methods. The overall prevalence of Salmonella was 9.3% (14/151). Breed, age, body condition, lactation stage, and parity were statistically significant risk factors ( ). Salmonellosis was more common and statistically significant in dairy cows with poor body condition and late lactation stage, as well as the Holstein Friesian crossbreed, accounting for 17.6%, 19.1%, and 17.3%, respectively. The farm’s husbandry hygiene and management system, on the other hand, had no significant association with salmonellosis ( ). Salmonellosis was generally considered to be moderately prevalent and was one of the diseases of dairy cows in the study area that could have an impact on dairy production and have serious health and financial repercussions. As a result, improvements in milk quality maintenance and assurance are encouraged, and the need for additional research in the study area was suggested along with other ideas.

Salmonellosis is a bacterial infection caused by salmonella, a member of the Enterobacteriaceae family. From December 2021 to May 2021, a cross-sectional study was carried out to isolate Salmonella from poultry farms in the towns of Bishoftu and Adama and to determine the antimicrobial susceptibility frequency of the isolates. A total of 384 samples were tested for the presence of Salmonella, including 259 feces, 56 eggs, and 69 types of meat, using the ISO, 2002 standard procedures. The raw data were organized, coded, and entered into an Excel spreadsheet before being analyzed with STATA via descriptive analysis with chi-square. From 384 collected samples, 62 (16.15%) isolates were obtained, with 9.9%, 3.65, and 2.6% found in feces, eggs, and meat, respectively. Statistically, there was a significant difference between breeds ( value = 0.036). Bovines had the highest prevalence (32.83%), while Saso had the lowest (30.81%). The variation within each sample type, housing condition, and age group was not statistically significant ( value >0.05). Antimicrobial resistance was found in 29 (96.77%) of the isolates. Ampicillin and sulphamethoxazole-trimethoprim were effective against all isolates. Salmonella was isolated from various locations, sample types, ages, and breeds, indicating a wider distribution. Salmonellosis detection isolates suggested that it could be an emerging poultry and public health issue. As a result, future research should concentrate on isolating and identifying salmonella from poultry in backyard systems and comparing it to that of an intensive farm, as well as molecular characterization for serotyping and genetic studies, as well as genes responsible for salmonella pathogenicity and drug resistance.

Background: Klebsiella pneumoniae is an opportunistic infection that causes production losses and death in the chicken industry. A cross-sectional study was conducted on exotic chicken breeds reared at the Jigjiga poultry farm from November 2022 to May 2023 to estimate the occurrence, associated risk factors, and antimicrobial susceptibility profiles of Klebsiella pneumoniae. The chickens were selected using systematic random sampling techniques. A total of 384 cloacal swabs were collected aseptically and transported to the laboratory for analysis. For statistical analysis, STATA® version 14.0 statistical software was used. Results: From 384 examined faecal samples, 258 (67.2%) prevalences of Klebsiella pneumoniae were found. Furthermore, the association of the study’s risk factors with the prevalence of Klebsiella pneumoniae was explored, and no statistically significant association was identified between sex and age. Nonetheless, relative prevalence at the age level was higher in chickens aged 12 months (67.6%) and Sasso breeds (90.0%). Similarly, male chickens and those raised for meat and egg production had a high prevalence rate of 72.5 and 75.8%, respectively. A total of 30 isolated Klebsiella pneumoniae colonies were tested in vitro for antibiotic sensitivity for six drugs, and it was shown that Klebsiella pneumoniae is moderately sensitive to Penicillin G (43.3%) while having higher resistance to Oxytetracycline (80.0%). Conclusions: The current findings revealed that the research area had the highest prevalence of Klebsiella pneumoniae, and the isolates were resistant to commonly used drugs in the study area. Thus, a long-term intervention plan, thorough research to determine a nationwide status, as well as further multi-drug resistance patterns and molecular characterization, were urged.

Trematodes are chronic, debilitating diseases in livestock, causing significant economic losses worldwide. From mid-December 2021 to May 2022, a cross-sectional study was carried out in the Damot Sore District to estimate the prevalence of trematode infections in cattle and associated risk factors. Trematode eggs were found in 100 of the 384 faecal samples tested, with an overall prevalence of 26.04% (95% CI: 21.88-30.69%). The prevalence for Fasciola, Paramphistome, and Schistosoma species were 12.50%, 5.21%, and 0, respectively. Moreover, the infection rate with two parasites was 8.33%. The data were then examined further using univariable and multivariable logistic regression analysis. As a result, age was the only potential predictor identified to influence trematode infections in cattle among the potential predictors considered. Furthermore, old cattle were more likely to be infected with trematodiasis nearly 12 times ( ) that of young cattle, and this difference was statistically significant ( ), whereas other risk factors considered were statistically insignificant ( ). According to the findings of this study, cattle trematodiasis is a moderately common disease in the study area. As a result, additional research on the meteorological conditions of snail infection was forwarded, along with other points to reduce the disease problem in livestock production.

Background: Cysticercus bovis has zoonotic implications with economic losses from organ contamination and treatment costs. In developing countries such as Ethiopia, where hygienic standards are low and residents customarily eat raw or undercooked meat, the disease is prevalent. Methods: A cross-sectional study was carried out from December 2021 to June 2022 to determine the prevalence of Cysticercus bovis in cattle slaughtered at the Bishoftu municipal abattoir, in central Ethiopia. Study populations for the active abattoir survey were cattle presented to the abattoir for slaughtering and routine meat inspection conditions. The organ namely the tongue, masseter muscle, liver, and triceps muscle were inspected for the presence of C. bovis. Results: From a total of 330 cattle, 14 were found to be positive for C. bovis, with an overall prevalence of 4.24%. Based on the origin of the animals, the highest prevalence was recorded at Adama (7.27%), followed by Bishoftu (5.45%), Mojo (5.45%), Borana (3.63%), Dukem (3.63%), and Kaliti (0.00%). Similarly, out of 111 adults and 219 old-aged cattle slaughtered and examined at the abattoir, 4.5%, and 4.11% were positive for C. bovis, respectively. Among tested independent variables, sex, body condition score, age, and origin of animals had no relationships with the prevalence of C. bovis. From the detected organ, the tongue ranks first for the highest number of cysts, followed by the masseter muscle, liver, and triceps muscle, with total cysts of 6, 4, 3, and 1, respectively. Conclusion: C. bovis is a prevalent zoonotic parasite disease that causes carcass condemnation; an enhanced understanding of the health consequences of teniasis is required to protect the community.