Juliana Milheiro

Dr. Juliana Milheiro

orcid

Research Focus & Metrics

Academic Career Length

10Years (2017-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

View All Publications
28 May 2024 in Food Research International
Verified
Journal Article
Open access
LMLuís Miguel de Aquino Moreira
Luís Miguel de Aquino Moreira
JMJuliana Milheiro
Juliana Milheiro
LFLuís Filipe-Ribeiro
Luís Filipe-Ribeiro
FCFernanda Cosme
Fernanda Cosme
Fernando M. NunesFernando M. Nunes
Fernando M. Nunes
Fermented beverages, including wine, can accumulate high concentrations of biogenic amines (BAs), which can pose potential health risks. BAs are produced by various yeasts and lactic acid bacteria (LAB) during winemaking. LAB are the main contributors to the formation of histamine and tyramine, the most toxic and food safety relevant biogenic amines. Numerous factors, ranging from agricultural and oenological practices to sanitation conditions, can contribute to the formation of BAs in wines. Moreover, organic and biodynamic wines impose limitations on the use of common food additives employed to control the proliferation of native and spoilage microorganisms during vinification and storage. To mitigate histamine production, commercial starter cultures incapable of synthesising histamine have been effectively utilised to reduce wine histamine content. Alternative fermentative microorganisms are currently under investigation to enhance the safety, quality, and typicity of wines, including indigenous LAB, non-Saccharomyces yeasts, and BAs degrading strains. Furthermore, exploration of extracts from BAs-degrading microorganisms and their purified enzymes has been undertaken to reduce BAs levels in wines. This review highlights microbial contributors to BAs in wines, factors affecting their growth and BA production, and alternative microorganisms that can degrade or avoid BAs. The aim is to lessen reliance on additives, providing consumers with safer wine choices.

8 April 2017 in Data in Brief
Verified
Journal Article
Open access
PDF file
Luís Filipe-RibeiroLuís Filipe-Ribeiro
Luís Filipe-Ribeiro
JMJuliana Milheiro
Juliana Milheiro
CMCarlos Matos
Carlos Matos
Fernanda CosmeFernanda Cosme
Fernanda Cosme
Fernando M. NunesFernando M. Nunes
Fernando M. Nunes
Data in this article presents the changes on phenolic compounds, headspace aroma composition and sensory profile of a red wine spiked with 4-ethylphenol and 4-ethylguaiacol and treated with seven activated carbons with different physicochemical characteristics, namely surface area, micropore volume and mesopore volume (“Reduction of 4-ethylphenol and 4-ethylguaiacol in red wine by activated carbons with different physicochemical characteristics: impact on wine quality” Filipe-Ribeiro et al. (2017) [1]). Data on the physicochemical characteristics of the activated carbons are shown. Statistical data on the sensory expert panel consistency by General Procrustes Analysis is shown. Statistical data is also shown, which correlates the changes in chemical composition of red wines with the physicochemical characteristics of activated carbons used.

2 September 2023 in Foods
Verified
Journal Article
Open access
PDF file
LDLara Dias
Lara Dias
JMJuliana Milheiro
Juliana Milheiro
MRMiguel Ribeiro
Miguel Ribeiro
CFCristina Fernandes
Cristina Fernandes
NNNuno Neves
Nuno Neves
Fernando M. NunesFernando M. Nunes
Fernando M. Nunes
Wine phenolic compounds, particularly proanthocyanidins (PAs), play a significant role in wine sensory characteristics, specifically bitterness and astringency. Although not consensual, flavan-3-ols and oligomeric PAs are generally considered the primary contributors to wine bitterness. Patatin, a vegetable protein fining agent, has been explored as an alternative to animal and synthetic fining agents for reducing wine bitterness. However, contradictory results exist regarding its effectiveness in removing flavan-3-ols and oligomeric PAs in red wines. In this work, a UPLC–Q-TOF MS/MS method was optimized and validated for accurately measuring flavan-3-ols, as well as dimeric and trimeric PAs, in red wines. The MS/MS analysis of flavan-3-ols, in addition to the typical fragmentation described in the literature, revealed an intense mass fragment resulting from the loss of C3O2 and C3O2 + H2O from the parent ion. It was observed that flavan-3-ols and PAs undergo oxidation during sample preparation, which was reversed by the addition of 5 g/L of ascorbic acid. The method demonstrated good linearity range (2 mg/L to 20 mg/L), detection limit (0.3 mg/L to 0.7 mg/L), quantification limit (0.8 mg/L to 2.2 mg/L), precision (repeatability 2.2% to 7.3%), and accuracy (recovery 98.5% to 100.5%). The application of patatin at different doses (5 g/L to 30 g/L) in two different red wine matrices did not reduce the levels of monomeric, dimeric, and trimeric PAs in red wines. However, similar behaviors were observed for pea protein and gelatin. Therefore, wine fining trials and efficiency measurements of the treatments in each matrix are strongly advised.

30 December 2025 in Food Control
Verified
Journal Article
Open access
ARANA JACINTA ESCRIVÃES RIBEIRO
ANA JACINTA ESCRIVÃES RIBEIRO
JMJuliana Milheiro
Juliana Milheiro
Fernando M. NunesFernando M. Nunes
Fernando M. Nunes
TCTeresa B. de Carvalho
Teresa B. de Carvalho
Joana BarbosaJoana Barbosa
Joana Barbosa
CSCristina M. Saraiva
Cristina M. Saraiva

10 February 2021
Verified
Book Chapter
Open access
PDF file
JMJuliana Milheiro
Juliana Milheiro
Fernanda CosmeFernanda Cosme
Fernanda Cosme
Luís Filipe-RibeiroLuís Filipe-Ribeiro
Luís Filipe-Ribeiro
Fernando M. NunesFernando M. Nunes
Fernando M. Nunes
Port wine is a traditional and worldwide renowned fortified wine produced in the Douro Demarcated Region (DDR) Northeast of Portugal by specific and traditional winemaking practices. The final quality and uniqueness of the different Port wine styles are highly dependent on the ageing process, namely time, temperature and oxygen levels that will ultimately dictate the type and extension of the chemical changes that occur during this process. These chemical changes occurring during the Port wine ageing process results in significant changes in colour and aroma according to the different ageing conditions employed for the different Port wine styles. This chapter intends to give a broad and generic overview of the known and potential chemical changes occurring during ageing of Port wine that are responsible for the changes in the sensory profile observed during the ageing process. Also, the known chromatic and aromatic characteristics of the different Port wine styles and the specific ageing processes, reductive or oxidative, are reviewed.

28 January 2026 in Polymers
Verified
Journal Article
Open access
PDF file
LMLuís Miguel de Aquino Moreira
Luís Miguel de Aquino Moreira
JMJuliana Milheiro
Juliana Milheiro
Fernanda CosmeFernanda Cosme
Fernanda Cosme
Fernando M. NunesFernando M. Nunes
Fernando M. Nunes
Global chestnut production is rising. However, the Portuguese chestnut industry still experiences annual post-harvest losses, largely due to microbial spoilage. Recovering high-value starch from spoiled chestnuts offers a promising strategy to reduce waste and increase economic returns. Yet, starch extracted from spoiled kernels is typically dark brown, limiting its industrial applications. This study aimed to enhance the sustainability of the chestnut sector by converting industrial byproducts into useful ingredients. We evaluated whether hypochlorite-mediated oxidative extraction at pHs around 8 and 12 could produce starch with functional properties suitable for industrial applications. Both native and bleached starches showed similar lightness (L* 84–91), though a slight yellow hue remained (ΔE* 12–19). The degree of crystallinity was higher in bleached starches (13–16%) while preserving the characteristic CB-type crystalline pattern of native chestnut starch. The degree of oxidation was 0.88% and 0.43% for bleached starches isolated at pHs 8 and 12, respectively. Starch bleached at pH 8 exhibited moderate viscosity (breakdown 0.103) and greater swelling capacity at 50 °C than corn starch. In contrast, extraction under alkaline conditions markedly reduced gelatinization and retrogradation performance. Therefore, oxidative extraction at middle pH proved to be the most effective method for recovering functional starch from spoiled chestnuts.