Analysis of the relationships among ecosystem services (ESs) can help ensure that benefits from ecosystems are consistent over time. This study explored the spatial and temporal patterns of water supply (WS), grain supply (GS), carbon storage (CS), water conservation (WC), soil conservation (SC), and habitat quality (HQ) in the Wujiang River Basin (WJRB) from 2000 to 2020 and the trade-off/synergy relationships of ESs. The ESs in the WJRB are downstream > midstream > upstream in space, with the greatest increase and decrease in the upstream and midstream temporal dimensions, respectively. The WS, WC, and SC underwent a trend shift in 2005 due to climatic influences, whereas GS and HQ underwent a trend shift in 2010 due to human social development. GS formed a trade-off with other ESs in the spatial pattern, whereas WS formed a trade-off with CS and WC in the temporal dimension. Adjusting the GS spatial pattern reduces the trade-off between ESs in the spatial pattern, allowing for focusing on monitoring soil and water erosion-prone areas to prevent extensive soil erosion during heavy precipitation years; this reduces the trade-off between ESs in the time dimension in the WJRB. This provides a theoretical basis for achieving high-quality WJRB development.

Systematic biodiversity and ecosystem service (ES) conservation is vital for ecological sustainability and human well-being. This study combines MaxEnt, Zonation, InVEST, and MSPA models to identify Conservation Priority Areas (CPAs) in the Wujiang River Basin (WJRB), integrating biodiversity hotspots, ESs, and landscape connectivity. Results reveal CPAs span 1.13 × 10⁴ km² (primarily downstream), but existing natural reserves (NRs) cover only 24.86% of these critical zones, leaving over 75% unprotected in this region. Current NRs occupy 0.62 × 10⁴ km², with 5.82% of the basin (mainly upstream) available for targeted expansion. Spatial analysis reveals mismatches, such as some NRs protecting low-value ecological areas, resulting in imbalanced coverage. Expanding NRs across the board is less effective than adjusting protection scope or management strategies in areas of spatial mismatch, based on identified CPAs. This can involve establishing new reserves and appropriately relaxing land-use restrictions to allow compatible activities within them. New conservation planning should prioritize large, interconnected CPA regions to enhance landscape coherence. Simultaneously, integrating ecological compensation mechanisms can align protection goals with local livelihood improvements, fostering community engagement. This approach addresses critical gaps and enhances conservation efficiency by strategically directing resources toward high-value, vulnerable ecosystems. The methodology offers a replicable framework for balancing ecological preservation and human needs in river basin management.

While genetically modified crops bring significant economic benefits, the environmental safety issues they may pose have also received increasing attention. To study the impact of planting genetically modified insect-resistant crops on soil ecosystems, this research employed methods such as 16S rDNA amplicon full-length sequencing, using transgenic Cry1Ah insect-resistant corn HGK60 and its conventional counterpart Zheng 58 as subjects for a three-year continuous survey to analyze the effects of planting transgenic Cry1Ah insect-resistant corn HGK60 on the rhizosphere bacterial community. The following results were obtained. (1) A total of 216 corn rhizosphere soil samples were annotated to 51 phyla, 119 orders, 221 families, and 549 genera. (2) Overall, there was no significant difference in the composition of the rhizosphere bacterial community between HGK60 and Zheng 58 at the phylum, class, order, or family levels (p > 0.05), and the planting of HGK60 did not significantly affect the relative abundance of rhizosphere probiotics (p > 0.05). Some differences appeared only briefly and were not reproducible. (3) Alpha and beta diversity analyses showed that overall, the planting of HGK60 had no significant impact on the structure of the rhizosphere bacterial community (p > 0.05). (4) Significant changes in the rhizosphere bacterial community were observed across different growth stages of corn. It can be concluded that the planting of HGK60 has no significant impact on the rhizosphere bacteria. This study provides valuable data support for the environmental safety assessment of genetically modified crops.