Using the internal transcribed spacer (ITS) region for identification, three strains of Aspergillus terreus were identified and designated AUMC 15760, AUMC 15762, and AUMC 15763 for the Assiut University Mycological Centre culture collection. The ability of the three strains to manufacture lovastatin in solid-state fermentation (SSF) using wheat bran was assessed using gas chromatography-mass spectroscopy (GC-MS). The most potent strain was strain AUMC 15760, which was chosen to ferment nine types of lignocellulosic waste (barley bran, bean hay, date palm leaves, flax seeds, orange peels, rice straw, soy bean, sugarcane bagasse, and wheat bran), with sugarcane bagasse turning out to be the best substrate. After 10 days at pH 6.0 at 25 °C using sodium nitrate as the nitrogen source and a moisture content of 70%, the lovastatin output reached its maximum quantity (18.2 mg/g substrate). The medication was produced in lactone form as a white powder in its purest form using column chromatography. In-depth spectroscopy examination, including ¹H, ¹³C-NMR, HR-ESI-MS, optical density, and LC-MS/MS analysis, as well as a comparison of the physical and spectroscopic data with published data, were used to identify the medication. At an IC₅₀ of 69.536 ± 5.73 µM, the purified lovastatin displayed DPPH activity. Staphylococcus aureus and Staphylococcus epidermidis had MICs of 1.25 mg/mL, whereas Candida albicans and Candida glabrata had MICs of 2.5 mg/mL and 5.0 mg/mL, respectively, against pure lovastatin. As a component of sustainable development, this study offers a green (environmentally friendly) method for using sugarcane bagasse waste to produce valuable chemicals and value-added commodities.

Black aspergilli are excellent candidates for a variety of secondary metabolites. In this investigation, Aspergillus tubingensis AUMC 15759 produced certain bioactive compounds in solid-state fermentation (SSF) utilizing some agricultural wastes, namely bagasse, barley bran, bean hay, date palm leaves, flax seeds, orange peels, rice straw, soybean, and wheat bran. Rice straw extract yielded the highest inhibition zones of 14.1±0.2, 13.6±0.85, 12.0±0.9, and 15.0±0.9 mm, against Candida albicans, Candida glabrata, Candida krusei, and Saccharomyces cerevisiae. The rice straw extract demonstrated impressive antioxidant activity (%DPPH) that was nearly similar to that of ascorbic acid exhibiting IC50 of 1.2±0.2 mg/ml. The liquid/liquid fractionation approach of the rice straw crude extract's liquid yielded five fractions, with the dichloromethane fraction being the most effective. GC-MS analysis disclosed the identification of nine substances linked to phenolic chemicals (3,4,5-trimethoxy phenol, 4-((1E)-3-Hydroxy-1-propenyl)-2-methoxyphenol, Phi. -Aspidinol, m-Aminophenylacetylene, and 1-Phenyl-2-propene), fatty acids (Palamitic acid and cis,cis-Linoleic acid), quinone derivative (2,6-Dimethyl-3-(methoxymethyl)-p-benzoquinone), and alkaloid (N-Didehydrohexacarboxyl-2,4,5-trimethylpiperazine). The dichloromethane fraction displayed potent antifungal activity. The MIC was 6.25 mg/ml, with inhibition zones of 14.0±0.4, 15.5±0.7, 14.0±0.4, and 14.2±0.2 mm against the investigated fungi, respectively. It also had the lowest IC50 (1.4±0.2) when compared to the remaining fractions of n- butanol (1.8±0.4), ethyl acetate (1.5±0.6), n-hexane (11.5±0.2), and water (13.5±1.1), or ascorbic acid (10.64±0.84) mg/ml. Finally, this fungus displayed antifungal and antioxidant activities when grown on agricultural wastes. It can be concluded that the tested strain of A. tubingensis displayed antifungal and antioxidant activities when grown on agricultural wastes, particularly rice straw.