Acinetobacter baumannii (A. baumannii) is an example of an opportunistic pathogen that is generally harmless to healthy individuals but can cause serious infections, such as ventilator-associated pneumonia, wound infections, and bacteremia, in critically ill hospital patients. A. baumannii produces many proteins within its genome. By analyzing its structural and functional interpretations, bioinformatics techniques can make it easier to understand this organism. The protein is still unclear, though. As a result, this study developed an in-silico method for functional and structural characterization of the uncharacterized protein (accession ID: SSI32830.1). These provide many characteristics in silico viewpoints, such as the protein’s physiochemical qualities, subcellular localization, three-dimensional structure, and protein-protein interactions. Protein-protein interactions are explained using the STRING software. The projected tertiary structure evaluation was conducted using the Swiss Model. The best materials are chosen utilizing structural analyses based on Ramachandran plot analysis. This research sought to understand the function of A. baumannii. Therefore, this investigation will increase our understanding of pathophysiology and allow us to target the protein complex specifically.