The wilting process is required for the preparation of traditional low-moisture silage, which is not only subject to the interference of rainfall during the harvest season in many areas, but also increases labor, economic, and time costs. Therefore, in this experiment, newly harvested alfalfa was supplemented with a complex additive consisting of lactic acid bacteria (LAB), sucrose, and corn flour, and then ensiled directly in order to explore a suitable high-moisture silage processing method. There were four groups: control (CK), 0.01 g/kg LAB plus 2% sugar (S), 0.01 g/kg LAB plus 5% corn flour (C5), and 0.01 g/kg LAB plus 5% corn flour (C10). The results show that all four types of silage had significantly reduced acid detergent fiber, acetic acid content, and ammonia/total nitrogen (p < 0.05). The relative abundance of Lactobacillus spp. was significantly higher (p < 0.05), and that of Weissella, Clostridium sensu stricto 12, and Pantoea genera was significantly lower (p < 0.05) in all four treatments. Group S had significantly increased crude protein content, and groups C5 and C10 had significantly reduced neutral detergent fiber. Overall, group C10 had the highest LA concentration, the highest relative abundance of Lactobacillus spp., and the lowest pH, ammonia/total nitrogen, neutral detergent fiber, and acid detergent fiber. Silage with a complex additive consisting of LAB + 10% corn flour is recommended to achieve good fermentation with high nutritional quality.

In order to explore the effects of different silage inoculants on the silage quality of alfalfa (Medicago sativa L.), this study utilized six groups of experimental treatments and five kinds of additive treatments: Xinlaiwang I straw silage (group A), Xinlaiwang I alfalfa silage (group B), Zhuanglemei silage starter culture (group C), Baoshiqing (group D), Kangfuqing S lactic acid bacteria silage (group E), and another blank control group (CK group, distilled water). The effect of silage on fermentation characteristics and nutritional value of Adina alfalfa silage was studied by membership function analysis. The main study variable was inoculant strains. Alfalfa silage was packed into polyethylene plastic vacuum bags in the laboratory and sealed for 60 days. The silage was divided into six treatment groups with three replicates per group. The fermentation performance and nutrient composition of the silage were determined. The results showed that compared with the control group, adding Xinlaiwang I alfalfa silage (group B) could significantly increase the contents of crude protein (CP) and lactic acid (LA) in alfalfa silage (p < 0.05), decrease the contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF), and decrease the pH and ammoniacal nitrogen/total nitrogen (AN/TN). The results showed that different inoculants could improve the silage quality of alfalfa to different extent, and Xinlaiwang I alfalfa silage had the best effect.