Food-deceptive flowers primarily use visual signals (such as color) to mimic model plants and deceive insects into achieving pollination. Paphiopedilum micranthum is a food-deceptive orchid that has a pink labellum and two purple petals with a yellow base and has been proven to be pollinated by bumblebees. However, the chemical and molecular bases of the floral color are not well understood. We conducted targeted metabolite profiling and transcriptomic analysis to determine the color signal and its genetic basis in P. micranthum. We found that both anthocyanins and carotenoids contribute significantly to the formation of floral color that determines the color signal. Higher concentrations of anthocyanins (cyanidin and peonidin) and carotenoids (primarily lutein and zeaxanthin) were detected in the petal compared to the labellum. The upregulation of structural genes of CHS, F3′H, DFR and ANS on the anthocyanin biosynthesis pathway in petals was identified, as well as three genes of LCYE, BCH, and CCD4 on the carotenoid biosynthesis pathway. Furthermore, we discovered that three R2R3-MYBs and one bHLH transcription factors were co-expressed with the expression of different genes. These genes and transcription factors may be responsible for the spatial color difference of P. micranthum. Our study emphasizes that the color of this food-deceptive orchids is achieved through specific genes and transcription factors associated with the pigment biosynthesis pathway.

The two species with lobed leaves, viz., Petrocosmea henryi and P. weiyigangii are very distinctive from other species of Petrocosmea. Here, the third species with lobed leaves, named Petrocosmea panzhouensis, from western Guizhou, China, is described. The new species is most similar to P. henryi on shape of leaf blade margin and straight filaments, but it is distinguished from the latter by shape of bracts, and shape and length of anthers. The new taxon is assessed as “Data Deficient” (DD), according to the IUCN standards.

Recently, pollution caused by the heavy metal Cd has seriously affected the environment and agricultural crops. While Sinapis alba is known for its edible and medicinal value, its tolerance to Cd and molecular response mechanism remain unknown. This study aimed to analyze the tolerance of S. alba to Cd and investigate its molecular response mechanism through transcriptomic and physiological indicators. To achieve this, S. alba seedlings were treated with different concentrations of CdCl₂ (0.25 mmol/L, 0.5 mmol/L, and 1.0 mmol/L) for three days. Based on seedling performance, S. alba exhibited some tolerance to a low concentration of Cd stress (0.25 mmol/L CdCl₂) and a strong Cd accumulation ability in its roots. The activities and contents of several antioxidant enzymes generally exhibited an increase under the treatment of 0.25 mmol/L CdCl₂ but decreased under the treatment of higher CdCl₂ concentrations. In particular, the proline (Pro) content was extremely elevated under the 0.25 and 0.5 mmol/L CdCl₂ treatments but sharply declined under the 1.0 mmol/L CdCl₂ treatment, suggesting that Pro is involved in the tolerance of S. alba to low concentration of Cd stress. In addition, RNA sequencing was utilized to analyze the gene expression profiles of S. alba exposed to Cd (under the treatment of 0.25 mmol/L CdCl₂). The results indicate that roots were more susceptible to disturbance from Cd stress, as evidenced by the detection of 542 differentially expressed genes (DEGs) in roots compared to only 37 DEGs in leaves. GO and KEGG analyses found that the DEGs induced by Cd stress were primarily enriched in metabolic pathways, plant hormone signal transduction, and the biosynthesis of secondary metabolites. The key pathway hub genes were mainly associated with intracellular ion transport and cell wall synthesis. These findings suggest that S. alba is tolerant to a degree of Cd stress, but is also susceptible to the toxic effects of Cd. Furthermore, these results provide a theoretical basis for understanding Cd tolerance in S. alba.

The polar regions host a diverse array of moss species that have evolved to thrive in extreme environments. These mosses exhibit remarkable adaptations, including tolerance to freezing temperatures, desiccation, and ultraviolet radiation. Despite their ecological significance, genomic data on these organisms are still limited, impeding our understanding of their evolutionary history and adaptive mechanisms in the context of climate change. In this study, we present the first chromosome-scale genome assembly and annotation of the Arctic moss Ptychostomum knowltonii. The assembled genome is 408.8 Mb in size, anchored to 12 pseudochromosomes, with a scaffold N50 of 32.61 Mb. Repetitive elements account for 56.24% of the genome. The genome contains 28,014 protein-coding genes, with a BUSCO completeness of 96.20%. This genomic resource will enable future comparative genomic studies, enhancing our understanding of how polar mosses may respond to a warming climate and shedding light on their evolutionary trajectories in persistently extreme environments.