Wheat bran, abundant but underutilized, was investigated for its potential as a reinforcement in biocomposites through different pretreatment methods. Pretreatment methods included were dilute sodium hydroxide (NaOH), dilute sulfuric acid (H₂SO₄), liquid hot water (LHW), calcium hydroxide (CaOH), organosolv such as aqueous ethanol (EtOH), and methyl isobutyl ketone (MIBK). Changes in chemical composition and fiber characteristics of the treated bran were studied using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Cellulose content increased to 35.1% and 29.6% in brans treated with H₂SO₄ and NaOH, respectively. The SEM micrographs showed surface cleaning of treated bran while maintaining sufficient surface roughness for the H₂SO₄, NaOH, and MIBK treated brans. Crystallinity index increased slightly for all treatments except H₂SO₄. NaOH and H₂SO₄ pretreated brans achieved important fiber characteristics, which could be useful for making thermoplastic biocomposites. Innovative use of bran in thermoplastic will create more opportunities for growers while enhancing biodegradability.

Triticum aestivum (2n = 6x = 42, AABBDD) with Triticum longissimum (2n = 2x = 14; S¹S¹) cytoplasm ((lo) cytoplasm) has normal fertility and plant vigor. However, the nucleus of durum wheat (Triticum turgidum (2n = 4x = 28, AABB)) is incompatible with the T. longissimum cytoplasm, producing non-viable progeny. This incompatibility is alleviated by scs^ae, a species cytoplasm-specific (scs) gene, on the long arm of chromosome 1D (1DL) of common wheat. The hemizygous (lo) durum scs^aeline is male sterile and is maintained by crossing to normal durum wheat. After pollination, the seeds produced are either plump and viable (with scs^ae) or shriveled and inviable (without scs^ae). Thus, the chromosome with scs^aeis inherited as a whole without recombination. The objectives of this study were to characterize the chromosome carrying scs^aeand to determine the process through which this gene was introgressed into the (lo) durum background. Molecular marker analysis with 27 probes and primers mapped to homoeologous group 1 and genomic in situ hybridization using differentially labeled total genomic DNA of durum wheat and Aegilops tauschii suggest the presence of a 1AL segment in place of the distal region of 1DL. Owing to the absence of any detectable duplications or deletions, homoeologous recombination is the most likely mechanism by which this introgression occurred.Key words: homoeologous recombination, in situ hybridization, nuclear-cytoplasmic interaction, species cytoplasm specific gene

Multiple chronic conditions (MCCs) such as diabetes, hypertension, heart disease, arthritis, asthma, and common respiratory problems are prevalent in over one-fourth of Americans, and separate drugs are prescribed to manage each of the diseases. The nutritive crop seeds loaded with multiple drugs could be a cheap and sustainable alternative to drugs produced by pharmaceutical companies. Our long-term goal is to produce chickpea seeds containing comparable dosages of multiple drugs regularly prescribed for managing MCC. In this work, we conducted experiments to understand the uptake and translocation of metformin into the tissues of chickpea to demonstrate the applicability of LC–HR-ToF-MS in determining metformin concentration, and to investigate responses of increased dosage of metformin and it’s accumulation into the chickpea seed. We treated the chickpea plants with 100 and 500 mg/L metformin chloride and analyzed its concentration in the leaf, stem, and seeds. We observed that metformin was successfully uptaken by chickpeas plant and translocated to stem, leaf, and seeds in both treatments. We also observed that the metformin concentration is responsive and as high as 349 times increase in seed when the dosage was increased from 100 to 500 mg/L.

Biochar is a carbon-rich, porous substance produced from the thermal degradation process of carbon-based materials, like biomass and other solid waste, in an oxygen-deprived environment. The type of parent material and the conditions for processing are the principal factors in determining the properties of biochar. Because of its diverse physicochemical properties, biochar has gained growing attention over the decades as a cost-effective, sustainable, and emerging material with potential applications in energy, agriculture, and environmental sectors like wastewater treatment. Two different parent materials, such as wheat bran and maple leaf, were pyrolyzed at three different temperatures (300 °C, 500 °C, and 700 °C). The resultant biochar was analyzed for its adsorptive potential for different contaminants. All the tested physicochemical property values of the maple (Acer) leaf biochar were found to be higher than wheat (Triticum) bran biochar except bulk density and the dye absorption potential. Based on the biochar physiochemical properties, the pyrolysis temperature of 700 °C was found to be the best for pyrolyzing these biomasses. Irrespective of the biochar types, pH 2.0 with a residence time of 90 min outperformed with an initial dye concentration of 0.05 mg/mL and a biochar application rate of 50 mg/mL. Furthermore, MLBC exhibited higher oil adsorption potential in comparison with that of WBC. The addition of WBC and MLBC to the polymer beads increases their dye absorption competence; therefore, this biochar can be a potential means of water treatment.

Arabinoxylans (AX) are functional biopolymers, the main non-starch polysaccharides in cereals and other plants. AX is composed of xylose and arabinose, and the ester-linkage of ferulic acid to arabinose confers its bioactive properties. The backbone of AX resembles that of glycosaminoglycans, a major component of the human extracellular matrix. This study explores the potential of wheat bran AX-based scaffolds as a novel platform for the growth and development of triple-negative breast cancer (TNBC) cells, an aggressive form of breast cancer. Importantly, patients face the worst prognosis due to the stemness of the TNBC cells and the formation of hypoxic cell clumps. Wheat bran constitutes 15–25% of the byproducts after milling and adds limited economic value. We have extracted AX from wheat bran (WBAX) and developed soft scaffolds with Na-alginate. The scaffolds were seeded with the triple-negative breast cancer cell line MDA-MB-231. Over 21 days, cell growth and development, cell migration within the hydrogels, and the formation of hypoxic regions within cell clumps were observed. These findings suggest that WBAX-based scaffolds provide a conducive environment for TNBC cell proliferation and development, offering a promising avenue for further research into cancer cell biology and potential therapeutic applications.