Microbial carbonate mineralization is widespread in nature and among microorganisms, and of vast ecological and geological importance. However, our understanding of the mechanisms that trigger and control processes such as calcification, i.e., mineralization of CO₂ to calcium carbonate (CaCO₃), is limited and literature on cyanobacterial calcification is oftentimes bewildering and occasionally controversial. In cyanobacteria, calcification may be intimately associated with the carbon dioxide-(CO₂) concentrating mechanism (CCM), a biochemical system that allows the cells to raise the concentration of CO₂ at the site of the carboxylating enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) up to 1000-fold over that in the surrounding medium. A comprehensive understanding of biologically induced carbonate mineralization is important for our ability to assess its role in past, present, and future carbon cycling, interpret paleontological data, and for evaluating the process as a means for biological carbon capture and storage (CCS). In this review we summarize and discuss the metabolic, physiological and structural features of cyanobacteria that may be involved in the reactions leading to mineral formation and precipitation, present a conceptual model of cyanobacterial calcification, and, finally, suggest practical applications for cyanobacterial carbonate mineralization.

Cyanobacteria of the genera Synechococcus and Prochlorococcus are the most abundant photosynthetic organisms on earth, occupying a key position at the base of marine food webs. The cynS gene that encodes cyanase was identified among bacterial, fungal, and plant sequences in public databases, and the gene was particularly prevalent among cyanobacteria, including numerous Prochlorococcus and Synechococcus strains. Phylogenetic analysis of cynS sequences retrieved from the Global Ocean Survey database identified >60% as belonging to unicellular marine cyanobacteria, suggesting an important role for cyanase in their nitrogen metabolism. We demonstrate here that marine cyanobacteria have a functionally active cyanase, the transcriptional regulation of which varies among strains and reflects the genomic context of cynS . In Prochlorococcus sp. strain MED4, cynS was presumably transcribed as part of the cynABDS operon, implying cyanase involvement in cyanate utilization. In Synechococcus sp. strain WH8102, expression was not related to nitrogen stress responses and here cyanase presumably serves in the detoxification of cyanate resulting from intracellular urea and/or carbamoyl phosphate decomposition. Lastly, we report on a cyanase activity encoded by cynH , a novel gene found in marine cyanobacteria only. The presence of dual cyanase genes in the genomes of seven marine Synechococcus strains and their respective roles in nitrogen metabolism remain to be clarified.

Bacteria acquire phosphate (P_i) by maintaining a periplasmic concentration below environmental levels. We recently described an extracellular P_i buffer which appears to counteract the gradient required for P_i diffusion. Here, we demonstrate that various treatments to outer membrane (OM) constituents do not affect the buffered P_i because bacteria accumulate P_i in the periplasm, from which it can be removed hypo-osmotically. The periplasmic P_i can be gradually imported into the cytoplasm by ATP-powered transport, however, the proton motive force (PMF) is not required to keep P_i in the periplasm. In contrast, the accumulation of P_i into the periplasm across the OM is PMF-dependent and can be enhanced by light energy. Because the conventional mechanism of P_i-specific transport cannot explain P_i accumulation in the periplasm we propose that periplasmic P_i anions pair with chemiosmotic cations of the PMF and millions of accumulated P_i pairs could influence the periplasmic osmolarity of marine bacteria.