Hematological inflammatory ratios such as the neutrophil-to-lymphocyte (NLR), monocyte-to-lymphocyte (MLR), platelet-to-lymphocyte (PLR), and systemic inflammatory index (SII) have recently demonstrated prognostic value in acute inflammatory diseases, neoplastic conditions, hepatic and gastrointestinal pathologies, as well as acting as biomarkers for the early detection of tumors and cardiorespiratory diseases. In addition, they can be easily calculated and have no additional cost. The purpose of this study was to establish reference ranges for these ratios in the canine population, thereby providing support for clinical practice. Data was collected from previous studies. The dogs included in the study had to have anamnesis and physical examination without abnormalities and within reference limits in laboratory results. A total of 156 samples were included and divided by sex and age. The following reference intervals were established: 1.3–7.1 for NLR; 0.1–0.3 for MLR; 40.4–271.1 for PLR, and 224.6–2191.7 for SII. Statistical significant difference was found between sexes and NLR, with males showing a higher ratio than females. Regarding age, statistical significance was found between juveniles and senior dogs for NLR. PLR and SII showed higher ratios in senior dogs compared to adults and juveniles. This study may serve as a guide in clinical practice for the interpretation of hematological inflammatory ratios.

The use of omeprazole as a preventive treatment for gastrointestinal ulcers in veterinary medicine has been questioned during previous years. The aim of the present study is to assess the long-term effect of omeprazole on cobalamin and serum gastrin levels in healthy dogs. Eighteen healthy dogs were included: 10 in the control group and 8 in the omeprazole group. Three samples were collected: before starting the treatment (T₀), 30 days after the start of treatment (T₁), and at 60 days (T₂). The mean cobalamin value (ng/L) in the control group was 481.4 (±293.70) at T₀, 481.4 (±170.21) at T₁, and 513.2 (±174.50) at T₂. In the omeprazole group, the values were 424.62 (±161.57) at T₀, 454.5 (±160.96) at T₁, and 414.87 (±127.90) at T₂. No statistically significant changes were detected in cobalamin levels between the three-time period in both study groups. These results agree with previous findings in felines but contrast with human medicine studies. The median gastrin values (pg/mL) in the control group were 62.45 [30.17–218.75] at T₀, 76.06 [30.67–199.87] at T₁, and 63.02 [35.81–176.06] at T₂. The median gastrin value in the omeprazole group was 67.59 [55.96–101.60] at T₀, 191.77 [75.31–1901.77] at T₁, and 128.16 [43.62–1066.46] at T₂. Statistically significant differences were detected (p = 0.008), indicating an increase in gastrin levels after initiating treatment with omeprazole. In conclusion, the increased levels of gastrin observed in this population underscore the importance of conducting a comprehensive clinical assessment to identify potential gastrointestinal disorders, particularly in consideration of the usage of omeprazole as a preventive treatment.

Many surgical techniques have been described in the literature to repair chronic tendon or ligament ruptures. Although direct approximation of the edges is the surgical technique of choice, the use of synthetic, fascia lata, semitendinosus muscle, and small intestinal submucosa grafts has been described to repair large defects or augment tenous repairs. The aim of this paper was to present the long-term outcome of two clinical cases using a common calcaneal tendon cadaver allograft with subsequent application of platelet-rich plasma for chronic ruptures diagnosed by ultrasound, with a chronic defect between both edges. Twenty-four months after common calcaneal tendon rupture and 12 mo after patellar ligament rupture, orthopedic follow-up of both patients showed complete functional recovery and ultrasound findings were consistent with correct integration of the graft in both cases.

Introduction: The study of early markers to detect kidney malfunction has increased in recent years since serum markers, such as creatinine increase when there is a 75% loss of renal mass. Urinary capillary electrophoresis (UCE) is an available laboratory technique that provides an easily interpretable electrophoretic pattern. This pattern in our study has been divided into five fractions as it is done in serum: fraction 1 migrating in the albumin zone, fraction 2 in the alpha₁-globulins zone, fraction 3 in the alpha₂-globulins zone, fraction 4 in the beta-globulins zone, and fraction 5 in the gamma globulins zone. UCE can be useful in the early diagnosis of renal disease.Material and methods: In this study, UCE was performed in dogs with azotemia and proteinuria due to chronic kidney disease (CKD) not related to Leishmania infantum (L. infantum) infection (G₁, n = 11) and dogs with CKD related to L. infantum infection (G₂, n = 17) and compared with reference intervals from healthy dogs (G₀, n = 123), with the aim of comparing their phoretograms and assessing changes in the fractions of the phoretograms based on the health status of individuals.Results: Fraction 2 was statistically augmented in dogs with CKD (G₁) when compared with the healthy population (G₀) and dogs infected by L. infantum (G₂). Fraction 3 was statistically increased in dogs with CKD (G₁) and dogs infected by L. infantum (G₂) compared with G_0. Fraction 4 was found to be statistically decreased in dogs with CKD (G₁) and dogs infected by L. infantum (G₂) compared with G_0. Fraction 5 was statistically higher in dogs with L. infantum (G₂) compared with G₀ and dogs with CKD (G₁). No statistical relationship was found between the protein to creatinine ratio and different fractions from the urinary phoretogram in the study population. No statistical relationship was found between serum and urine fractions in the study population.Discussion: The results of the present study suggest that UCE is a promising non-invasive technique that might be used as a part of the diagnostic and follow-up in dogs with kidney disease due to different pathologies.

The neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), and systemic immune-inflammation index (SII) are derived hematological indices reflecting systemic inflammatory balance in dogs with different serological statuses for canine leishmaniosis (CanL). The aim of this study was to describe and compare hematological ratios (HRs) between dogs seropositive and seronegative for Leishmania infantum and to assess their distribution across LeishVet stages. A retrospective multicenter case–control study was conducted, including 305 seropositive dogs and 305 seronegative controls from referral hospitals in Spain. Hematological data were retrospectively reviewed and analyzed. Significant differences were observed in NLR and MLR between seronegative control dogs and dogs with leishmaniosis, whereas no differences were found for PLR or SII. Median NLR and MLR values were higher in dogs with leishmaniosis compared to controls. In addition, comparisons across LeishVet stages revealed statistically significant differences for all evaluated HRs. These findings indicate that NLR and MLR are increased in dogs with CanL and that HR varies across LeishVet stages, as these indices represent the relative proportions of neutrophils and monocytes in relation to lymphocytes; therefore, changes in their values reflect shifts in leukocyte distribution observed across clinical stages.

Electrophoresis of urine to evaluate different urinary proteins has been used in recent years in veterinary medicine, as it can be a useful laboratory tool in the early detection of kidney damage. However, urinary capillary electrophoresis (UCE) has not been reported in healthy cats. In healthy dogs, reference intervals have been established and can be compared with pathological samples as these provide an easily interpretable pattern. The electrophoretogram in this study is divided into five fractions (F1–F5) by serum (albumin; alpha₁-globulin; alpha₂-globulin; beta-globulin; and gamma-globulin). Urine samples from 14 healthy cats were obtained by eco-guided cystocentesis. UCE was run in all samples and compared to 123 dog electrophoretograms from a previously published study. Fraction 2 (alpha₁-globulin) was statistically decreased in cats (G₁) compared to dogs (G₂). Fraction 4 (beta-globulin) was statistically augmented in cats compared to the canine population (G₂). Fraction 5 (gamma-globulin) was statistically decreased in cats (G₁) compared to dogs (G₂). No statistical correlation was found between each cat’s serum and urinary fractions. The results of the present study suggest that UCE patterns in cats are similar to the ones described in dogs. UCE can be a non-invasive new diagnostic tool in cats as pathological patterns can be compared to normal ones.