Microplastics are transferred to humans through the food chain by consuming food contaminated with microplastics. However, the knowledge about the risks of dietary exposure for humans to these particles is very limited. Moreover, only a few studies on microplastic pollution in fruit and vegetables have been carried on. Thus, this study aims to investigate the presence of microplastics in some of the most consumed fruits and vegetables (pear (Pyrus communis), apple (Malus domestica), tomato (Solanum lycopersicum), onion (Allium cepa), potatoes (Solanum tuberosum), and cucumber (Cucumis sativus)) from Turkey and to evaluate the potential risk for consumers. Fruits and vegetable samples were purchased from different markets and fruiterer (two of each) in Muğla province, Southwest of Turkey. Microplastic extraction processes were carried out on the edible parts of the samples. According to the results obtained, a total of 210 particles (2.9 ± 1.6 particle g⁻¹) were detected in all samples. Any significant difference occurred among the different markets. The maximum average amount of microplastic was determined in tomato samples (3.63 ± 1.39 particle g⁻¹). The highest microplastic intake was with tomato (398,520 particles individual⁻¹ year⁻¹ for Estimated Annual Intake (EAI) and Estimated Daily Intake (EDI) for children 68.24 particles kg⁻¹ day⁻¹). The occurrence of microplastics of big size, that are not allowed to pass by plant xylem transport, suggests that fresh vegetables and fruits can be contaminated with plastic, especially during the production phase, during agricultural activities and during the marketing process (transport to the market and purchasing process).

Aquafeed’s contamination by microplastics can pose a risk to fish health and quality since they can be absorbed by the gastrointestinal tract and translocate to different tissues. The liver acts as a retaining organ with the consequent triggering of oxidative stress response. The present study aimed to combine the use of natural astaxanthin with natural-based microcapsules to counteract these negative side effects. European seabass juveniles were fed diets containing commercially available fluorescent microplastic microbeads (1–5 μm; 50 mg/kg feed) alone or combined with microencapsulated astaxanthin (AX) (7 g/kg feed; tested for half or whole feeding trial—30 or 60 days, respectively). Fish from the different dietary treatments did not evidence variations in survival and growth performance and did not show pathological alterations at the intestinal level. However, the microplastics were absorbed at the intestinal level with a consequent translocation to the liver, leading, when provided solely, to sod1, sod2, and cat upregulation. Interestingly, the dietary implementation of microencapsulated AX led to a mitigation of oxidative stress. In addition, the microcapsules, due to their composition, promoted microplastic coagulation in the fish gut, limiting their absorption and accumulation in all the tissues analyzed. These results were supported by in vitro tests, which demonstrated that the microcapsules promoted microplastic coagula formation too large to be absorbed at the intestinal level and by the fact that the coagulated microplastics were released through the fish feces.

Dietary microplastics (MPs) can be consumed by fish, crossing through the gastrointestinal tract. MPs smaller than 20 μm can easily translocate to other organs, such as liver, commonly triggering oxidative stress in fish. Given the current unlikelihood of their short-term elimination, strategies to mitigate MPs-related issues on fish are of considerable interest to the scientific community. In the present study, to reduce both the dietary MPs-induced oxidative stress and the accumulation of MPs, the effectiveness of microencapsulated astaxanthin (ASX) was evaluated in zebrafish (Danio rerio). Specifically, zebrafish were reared from larvae to adults (6 months) and fed diets containing MPs different in range-size (polymer A: 1-5 μm; polymer B: 40-47 μm) at different concentrations (50 or 500 mg/kg). After this period, fish from each experimental group were divided in two sub-groups that were fed, for an additional month, with the previous diets or with the same diets containing implemented with microencapsulated ASX (7 g/kg), respectively. Results showed that microencapsulated ASX was able to counteract the negative effects caused by MPs different in size. Particularly, in zebrafish fed diets containing polymer B microbeads, microencapsulated astaxanthin was able to restore the intestinal epithelium, affected by the abrasive role of MPs during gut transit. Differently, in zebrafish fed diets containing polymer A microbeads, absorbed at intestinal level and translocated mainly to the liver, the microencapsulated ASX decreased the oxidative stress response and reduced the MPs accumulation in target organs due to the antioxidant and the coagulant properties of the ASX and microcapsules wall, respectively. Taken together, the results highlighted that the aquafeeds' implementation with microencapsulated astaxanthin is a prospective tool to prevent MPs-related issues in fish.

Microplastic (MP) contamination in aquafeed poses a significant risk to fish health and safety. This study evaluated the effectiveness of a microencapsulated natural antioxidant, astaxanthin (AX), in mitigating the adverse effects of dietary MPs in rainbow trout fry. The microcapsules were composed of an organic wall matrix designed to preserve AX while limiting MP absorption in the intestine. During a 60-day feeding trial, fish were fed diets containing amino formaldehyde polymer fluorescent MP microbeads (1–5 µm; 50 mg/kg), either alone or in combination with microencapsulated AX. MP localization in tissues was assessed via confocal microscopy, and quantification was performed following chemical tissue digestion. Fish welfare was evaluated using histological and molecular analyses. No significant effects on growth or gut morphology were observed across experimental groups. However, MPs were mainly translocated to the liver, where they induced oxidative stress, as evidenced by the upregulation of sod1, sod2, and cat gene expression. The inclusion of microencapsulated AX significantly mitigated the oxidative stress response, and the microcapsules facilitated MP coagulation in the gut, reducing intestinal absorption. These findings highlight the potential of microencapsulated antioxidants to counteract MP-induced oxidative stress and reduce MP bioavailability in aquaculture species, contributing to improved fish welfare and product quality.