The objective of this study was to verify, through statistical analysis, analysis of variance by the F test, regression analysis and multivariate analysis, the behavior of the nutrient contents of the biofertilizer in the soil. For this purpose, different levels of doses of liquid swine biofertilizer were used to identify which variables had relevant statistical significance, among the variables, pH, organic matter, phosphorus, potassium, calcium, magnesium, hydrogen + aluminum, aluminum, base saturation, boron, copper, iron, manganese and zinc. The soil used was the Red Latosol type. The option to use swine biofertilizer in this experiment is due to the fact that in Brazil swine farming activity generates large amounts of waste that must be used in agricultural production. The experiment was carried out with 30 plots (500-gram pots each) stored and treated in a climate-controlled greenhouse at a temperature between 28°C and 31°C, in the microbiology laboratory of the Universidade Prof. Edson Antônio Velano - Unifenas, Alfenas Campus, in a completely randomized design - DIC, with 5 treatments and 6 replicates. The liming and field moisture maintenance period was 70 days. The treatments were conducted with swine biofertilizer collected at the Retiro School Farm of Unifenas in Alfenas - MG. The proportion of soil used was 40 m3, 80 m3, 160 m3, 320 m3 and 640 m3 per ha-1 corresponding to 10 ml, 20 ml, 40 ml, 80 ml, and 160 ml of biofertilizer respectively, plus the control plot that had no biofertilizer added and was called 0ml. The amounts of biofertilizer were adopted after the first chemical analysis of the soil and swine biofertilizer. The Sisvar 19 software was used to collect and determine the data, perform statistical analysis, analysis of variance using the F test, regression analysis, determination of the coefficient (R2), and others. The results of the analysis of variance showed that there was a significant effect on some nutrients as the doses of biofertilizer were progressively increased. Thus, as the doses increased, the levels of the soil's nutritional load also increased. Therefore, the use of swine biofertilizer improved the structure and physical and chemical characteristics of the soil. Therefore, several types of plants could be cultivated after incorporating swine biofertilizer into the soil, with legumes, vegetables, and perennial crops, especially fruit, standing out in this case.